Restriction map for
pFN21AE3544
(Linear) (Six-base) MAPSORT of: KIEE3544.seq Check: 2748 from: 1 to: 1236
KIEE3544.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 553 1236
Size: 262 291 683
Fragments arranged by size:
683 291 262
AlwNI CAG_nnn'CTG
Cuts at: 0 1003 1236
Size: 1003 233
ApaI G_GGCC'C
Cuts at: 0 206 255 769 1236
Size: 206 49 514 467
Fragments arranged by size:
514 467 206 49
ApaLI G'TGCA_C
Cuts at: 0 346 1236
Size: 346 890
ApoI r'AATT_y
Cuts at: 0 1150 1236
Size: 1150 86
AvaI C'yCGr_G
Cuts at: 0 130 762 889 955 1057 1078 1236
Size: 130 632 127 66 102 21 158
Fragments arranged by size:
632 158 130 127 102 66 21
BamHI G'GATC_C
Cuts at: 0 59 1236
Size: 59 1177
BanI G'GyrC_C
Cuts at: 0 565 688 981 1014 1042 1073 1198 1236
Size: 565 123 293 33 28 31 125 38
Fragments arranged by size:
565 293 125 123 38 33 31 28
BanII G_rGCy'C
Cuts at: 0 131 206 255 506 769 904 1037 1058
Size: 131 75 49 251 263 135 133 21
Cuts at: 1058 1236
Size: 178
Fragments arranged by size:
263 251 178 135 133 131 75 49
21
BbeI G_GCGC'C
Cuts at: 0 569 692 1077 1202 1236
Size: 569 123 385 125 34
Fragments arranged by size:
569 385 125 123 34
BbsI GAAGACnn'nnnn_
Cuts at: 0 21 1236
Size: 21 1215
BbvCI CC'TCA_GC
Cuts at: 0 749 756 1236
Size: 749 7 480
Fragments arranged by size:
749 480 7
BglI GCCn_nnn'nGGC
Cuts at: 0 1012 1236
Size: 1012 224
Bme1580I G_kGCm'C
Cuts at: 0 206 255 350 769 984 1236
Size: 206 49 95 419 215 252
Fragments arranged by size:
419 252 215 206 95 49
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1236
Size: 318 918
Bpu10I CC'TnA_GC
Cuts at: 0 749 756 1031 1236
Size: 749 7 275 205
Fragments arranged by size:
749 275 205 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1236
Size: 340 896
BsaI GGTCTCn'nnnn_
Cuts at: 0 886 1236
Size: 886 350
BsaHI Gr'CG_yC
Cuts at: 0 566 689 806 1074 1199 1236
Size: 566 123 117 268 125 37
Fragments arranged by size:
566 268 125 123 117 37
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 306 813 874 1038 1236
Size: 148 158 507 61 164 198
Fragments arranged by size:
507 198 164 158 148 61
BseYI C'CCAG_C
Cuts at: 0 913 1063 1186 1204 1236
Size: 913 150 123 18 32
Fragments arranged by size:
913 150 123 32 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 1236
Size: 313 923
BsiEI CG_ry'CG
Cuts at: 0 38 1236
Size: 38 1198
BsiHKAI G_wGCw'C
Cuts at: 0 350 938 1236
Size: 350 588 298
Fragments arranged by size:
588 350 298
BsmBI CGTCTCn'nnnn_
Cuts at: 0 457 1236
Size: 457 779
Bsp1286I G_dGCh'C
Cuts at: 0 131 206 255 350 506 769 904 938
Size: 131 75 49 95 156 263 135 34
Cuts at: 938 984 1037 1058 1236
Size: 46 53 21 178
Fragments arranged by size:
263 178 156 135 131 95 75 53
49 46 34 21
BsrBI CCG'CTC
Cuts at: 0 24 1236
Size: 24 1212
BsrDI GCAATG_nn'
Cuts at: 0 158 1236
Size: 158 1078
BsrFI r'CCGG_y
Cuts at: 0 350 1006 1039 1236
Size: 350 656 33 197
Fragments arranged by size:
656 350 197 33
BsrGI T'GTAC_A
Cuts at: 0 701 1236
Size: 701 535
BstBI TT'CG_AA
Cuts at: 0 170 1236
Size: 170 1066
BstYI r'GATC_y
Cuts at: 0 59 668 1236
Size: 59 609 568
Fragments arranged by size:
609 568 59
Bsu36I CC'TnA_GG
Cuts at: 0 989 1163 1236
Size: 989 174 73
Fragments arranged by size:
989 174 73
BtgI C'CryG_G
Cuts at: 0 496 1022 1236
Size: 496 526 214
Fragments arranged by size:
526 496 214
BtsI GCAGTG_nn'
Cuts at: 0 20 1236
Size: 20 1216
EaeI y'GGCC_r
Cuts at: 0 35 782 1236
Size: 35 747 454
Fragments arranged by size:
747 454 35
EagI C'GGCC_G
Cuts at: 0 35 1236
Size: 35 1201
EarI CTCTTCn'nnn_
Cuts at: 0 205 1236
Size: 205 1031
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 72 358 661 701 897 1236
Size: 72 286 303 40 196 339
Fragments arranged by size:
339 303 286 196 72 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 318 553 1236
Size: 262 56 235 683
Fragments arranged by size:
683 262 235 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 1236
Size: 241 995
EcoO109I rG'GnC_Cy
Cuts at: 0 217 264 766 1236
Size: 217 47 502 470
Fragments arranged by size:
502 470 217 47
EcoRI G'AATT_C
Cuts at: 0 1150 1236
Size: 1150 86
FspI TGC'GCA
Cuts at: 0 472 1236
Size: 472 764
FspAI rTGC'GCAy
Cuts at: 0 472 1236
Size: 472 764
HaeII r_GCGC'y
Cuts at: 0 308 554 569 589 692 1077 1202 1236
Size: 308 246 15 20 103 385 125 34
Fragments arranged by size:
385 308 246 125 103 34 20 15
KasI G'GCGC_C
Cuts at: 0 565 688 1073 1198 1236
Size: 565 123 385 125 38
Fragments arranged by size:
565 385 125 123 38
MspA1I CmG'CkG
Cuts at: 0 374 498 917 1000 1204 1236
Size: 374 124 419 83 204 32
Fragments arranged by size:
419 374 204 124 83 32
NaeI GCC'GGC
Cuts at: 0 1008 1236
Size: 1008 228
NarI GG'CG_CC
Cuts at: 0 566 689 1074 1199 1236
Size: 566 123 385 125 37
Fragments arranged by size:
566 385 125 123 37
NgoMIV G'CCGG_C
Cuts at: 0 1006 1236
Size: 1006 230
PasI CC'CwG_GG
Cuts at: 0 769 1236
Size: 769 467
PfoI T'CCnGG_A
Cuts at: 0 143 1236
Size: 143 1093
PpuMI rG'GwC_Cy
Cuts at: 0 217 1236
Size: 217 1019
PspOMI G'GGCC_C
Cuts at: 0 202 251 765 1236
Size: 202 49 514 471
Fragments arranged by size:
514 471 202 49
PstI C_TGCA'G
Cuts at: 0 15 1236
Size: 15 1221
PvuII CAG'CTG
Cuts at: 0 917 1000 1236
Size: 917 83 236
Fragments arranged by size:
917 236 83
SacII CC_GC'GG
Cuts at: 0 499 1236
Size: 499 737
SanDI GG'GwC_CC
Cuts at: 0 217 1236
Size: 217 1019
SexAI A'CCwGG_T
Cuts at: 0 83 1236
Size: 83 1153
SfcI C'TryA_G
Cuts at: 0 11 1236
Size: 11 1225
SfoI GGC'GCC
Cuts at: 0 567 690 1075 1200 1236
Size: 567 123 385 125 36
Fragments arranged by size:
567 385 125 123 36
SgrAI Cr'CCGG_yG
Cuts at: 0 1039 1236
Size: 1039 197
SmaI CCC'GGG
Cuts at: 0 764 1236
Size: 764 472
SmlI C'TyrA_G
Cuts at: 0 355 1236
Size: 355 881
StuI AGG'CCT
Cuts at: 0 754 1236
Size: 754 482
TatI w'GTAC_w
Cuts at: 0 113 701 1236
Size: 113 588 535
Fragments arranged by size:
588 535 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 1236
Size: 370 32 834
Fragments arranged by size:
834 370 32
XmaI C'CCGG_G
Cuts at: 0 762 1236
Size: 762 474
Enzymes that do cut:
AcuI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BstBI BstYI
Bsu36I BtgI BtsI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI FspAI HaeII KasI MspA1I
NaeI NarI NgoMIV PasI PfoI PpuMI PspOMI PstI
PvuII SacII SanDI SexAI SfcI SfoI SgrAI SmaI
SmlI StuI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AscI AseI AsiSI
AvrII BaeI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BspMI BssHII BssSI BstAPI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoRV FalI FseI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SalI SapI SbfI ScaI SfiI
SgfI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII Tth111I XbaI XcmI XhoI XmnI ZraI