Restriction map for
pFN21AE3538
(Linear) (Six-base) MAPSORT of: KIEE3538.seq Check: 1917 from: 1 to: 2457
KIEE3538.orf
With 182 enzymes: SgfI *
July 3, 2012 18:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 725 2457
Size: 725 1732
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 1202 1430 2026 2457
Size: 327 875 228 596 431
Fragments arranged by size:
875 596 431 327 228
AflIII A'CryG_T
Cuts at: 0 2081 2457
Size: 2081 376
AleI CACnn'nnGTG
Cuts at: 0 733 980 2457
Size: 733 247 1477
Fragments arranged by size:
1477 733 247
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 323 563 1400 2457
Size: 323 240 837 1057
Fragments arranged by size:
1057 837 323 240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 668 700 2457
Size: 668 32 1757
Fragments arranged by size:
1757 668 32
AlwNI CAG_nnn'CTG
Cuts at: 0 117 922 1099 2292 2457
Size: 117 805 177 1193 165
Fragments arranged by size:
1193 805 177 165 117
ApaI G_GGCC'C
Cuts at: 0 223 1123 1326 2457
Size: 223 900 203 1131
Fragments arranged by size:
1131 900 223 203
ApaLI G'TGCA_C
Cuts at: 0 1593 2457
Size: 1593 864
ApoI r'AATT_y
Cuts at: 0 2163 2440 2457
Size: 2163 277 17
Fragments arranged by size:
2163 277 17
AvaI C'yCGr_G
Cuts at: 0 209 222 271 825 1225 1994 2457
Size: 209 13 49 554 400 769 463
Fragments arranged by size:
769 554 463 400 209 49 13
BanI G'GyrC_C
Cuts at: 0 213 322 1435 2457
Size: 213 109 1113 1022
Fragments arranged by size:
1113 1022 213 109
BanII G_rGCy'C
Cuts at: 0 223 890 1123 1166 1199 1326 1398 1790
Size: 223 667 233 43 33 127 72 392
Cuts at: 1790 2216 2457
Size: 426 241
Fragments arranged by size:
667 426 392 241 233 223 127 72
43 33
BbeI G_GCGC'C
Cuts at: 0 1439 2457
Size: 1439 1018
BciVI GTATCCnnnnn_n'
Cuts at: 0 1020 2457
Size: 1020 1437
BglI GCCn_nnn'nGGC
Cuts at: 0 211 948 2457
Size: 211 737 1509
Fragments arranged by size:
1509 737 211
BglII A'GATC_T
Cuts at: 0 1856 2457
Size: 1856 601
BlpI GC'TnA_GC
Cuts at: 0 523 1699 2457
Size: 523 1176 758
Fragments arranged by size:
1176 758 523
Bme1580I G_kGCm'C
Cuts at: 0 216 223 440 937 1123 1326 1597 2457
Size: 216 7 217 497 186 203 271 860
Fragments arranged by size:
860 497 271 217 216 203 186 7
BmgBI CAC'GTC
Cuts at: 0 585 1668 2457
Size: 585 1083 789
Fragments arranged by size:
1083 789 585
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 929 2082 2457
Size: 929 1153 375
Fragments arranged by size:
1153 929 375
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 220 301 352 462 610 779 853
Size: 199 21 81 51 110 148 169 74
Cuts at: 853 899 929 1076 2457
Size: 46 30 147 1381
Fragments arranged by size:
1381 199 169 148 147 110 81 74
51 46 30 21
Bpu10I CC'TnA_GC
Cuts at: 0 884 1367 1373 2457
Size: 884 483 6 1084
Fragments arranged by size:
1084 884 483 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1894 2457
Size: 1894 563
BsaI GGTCTCn'nnnn_
Cuts at: 0 666 825 1272 1510 2149 2457
Size: 666 159 447 238 639 308
Fragments arranged by size:
666 639 447 308 238 159
BsaAI yAC'GTr
Cuts at: 0 1707 1773 2457
Size: 1707 66 684
Fragments arranged by size:
1707 684 66
BsaBI GATnn'nnATC
Cuts at: 0 1006 1684 2457
Size: 1006 678 773
Fragments arranged by size:
1006 773 678
BsaHI Gr'CG_yC
Cuts at: 0 1436 1730 2457
Size: 1436 294 727
Fragments arranged by size:
1436 727 294
BsaWI w'CCGG_w
Cuts at: 0 1398 1796 2457
Size: 1398 398 661
Fragments arranged by size:
1398 661 398
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 668 698 1074 1104 2457
Size: 668 30 376 30 1353
Fragments arranged by size:
1353 668 376 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 459 487 1258 1261 2236 2457
Size: 459 28 771 3 975 221
Fragments arranged by size:
975 771 459 221 28 3
BseYI C'CCAG_C
Cuts at: 0 262 314 633 693 760 1103 1258 2183
Size: 262 52 319 60 67 343 155 925
Cuts at: 2183 2457
Size: 274
Fragments arranged by size:
925 343 319 274 262 155 67 60
52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 175 869 1406 1737 1864 1882 2457
Size: 103 72 694 537 331 127 18 575
Fragments arranged by size:
694 575 537 331 127 103 72 18
BsiEI CG_ry'CG
Cuts at: 0 1797 2457
Size: 1797 660
BsiHKAI G_wGCw'C
Cuts at: 0 529 1199 1597 1790 2457
Size: 529 670 398 193 667
Fragments arranged by size:
670 667 529 398 193
BsmI GAATG_Cn'
Cuts at: 0 703 2457
Size: 703 1754
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2353 2457
Size: 2353 104
Bsp1286I G_dGCh'C
Cuts at: 0 216 223 440 529 890 937 1123 1166
Size: 216 7 217 89 361 47 186 43
Cuts at: 1166 1199 1326 1398 1597 1790 2216 2457
Size: 33 127 72 199 193 426 241
Fragments arranged by size:
426 361 241 217 216 199 193 186
127 89 72 47 43 33 7
BspHI T'CATG_A
Cuts at: 0 1688 2457
Size: 1688 769
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 725 1886 2457
Size: 67 658 1161 571
Fragments arranged by size:
1161 658 571 67
BsrBI CCG'CTC
Cuts at: 0 183 2457
Size: 183 2274
BssSI C'ACGA_G
Cuts at: 0 903 1275 1312 2299 2457
Size: 903 372 37 987 158
Fragments arranged by size:
987 903 372 158 37
BstAPI GCAn_nnn'nTGC
Cuts at: 0 59 847 1878 2457
Size: 59 788 1031 579
Fragments arranged by size:
1031 788 579 59
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 679 735 982 1153 1318 2457
Size: 378 301 56 247 171 165 1139
Fragments arranged by size:
1139 378 301 247 171 165 56
BstYI r'GATC_y
Cuts at: 0 1001 1856 2335 2457
Size: 1001 855 479 122
Fragments arranged by size:
1001 855 479 122
Bsu36I CC'TnA_GG
Cuts at: 0 470 772 2457
Size: 470 302 1685
Fragments arranged by size:
1685 470 302
BtgI C'CryG_G
Cuts at: 0 71 2457
Size: 71 2386
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 2457
Size: 357 2100
BtsI GCAGTG_nn'
Cuts at: 0 662 2457
Size: 662 1795
DraIII CAC_nnn'GTG
Cuts at: 0 847 2288 2457
Size: 847 1441 169
Fragments arranged by size:
1441 847 169
EaeI y'GGCC_r
Cuts at: 0 711 1289 1578 1976 2457
Size: 711 578 289 398 481
Fragments arranged by size:
711 578 481 398 289
EarI CTCTTCn'nnn_
Cuts at: 0 346 491 560 1639 1795 2257 2457
Size: 346 145 69 1079 156 462 200
Fragments arranged by size:
1079 462 346 200 156 145 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 427 595 2457
Size: 427 168 1862
Fragments arranged by size:
1862 427 168
EcoICRI GAG'CTC
Cuts at: 0 1197 1788 2457
Size: 1197 591 669
Fragments arranged by size:
1197 669 591
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 220 301 327 352 462 610 779
Size: 199 21 81 26 25 110 148 169
Cuts at: 779 853 899 929 1076 1202 1430 2026 2457
Size: 74 46 30 147 126 228 596 431
Fragments arranged by size:
596 431 228 199 169 148 147 126
110 81 74 46 30 26 25 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 1336 2457
Size: 424 912 1121
Fragments arranged by size:
1121 912 424
EcoO109I rG'GnC_Cy
Cuts at: 0 186 276 336 919 940 1119 1322 1323
Size: 186 90 60 583 21 179 203 1
Cuts at: 1323 2457
Size: 1134
Fragments arranged by size:
1134 583 203 186 179 90 60 21
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 78 2031 2457
Size: 78 1953 426
Fragments arranged by size:
1953 426 78
HaeII r_GCGC'y
Cuts at: 0 1140 1439 1652 2457
Size: 1140 299 213 805
Fragments arranged by size:
1140 805 299 213
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1014 1046 1061 1093 1932 1964 2457
Size: 1014 32 15 32 839 32 493
Fragments arranged by size:
1014 839 493 32 32 32 15
HindIII A'AGCT_T
Cuts at: 0 1284 2327 2457
Size: 1284 1043 130
Fragments arranged by size:
1284 1043 130
KasI G'GCGC_C
Cuts at: 0 1435 2457
Size: 1435 1022
MscI TGG'CCA
Cuts at: 0 713 1291 1580 1978 2457
Size: 713 578 289 398 479
Fragments arranged by size:
713 578 479 398 289
MslI CAynn'nnrTG
Cuts at: 0 733 980 1639 2080 2107 2408 2457
Size: 733 247 659 441 27 301 49
Fragments arranged by size:
733 659 441 301 247 49 27
MspA1I CmG'CkG
Cuts at: 0 601 1099 1206 1751 2457
Size: 601 498 107 545 706
Fragments arranged by size:
706 601 545 498 107
NarI GG'CG_CC
Cuts at: 0 1436 2457
Size: 1436 1021
NcoI C'CATG_G
Cuts at: 0 71 2457
Size: 71 2386
NspI r_CATG'y
Cuts at: 0 93 159 520 2085 2112 2457
Size: 93 66 361 1565 27 345
Fragments arranged by size:
1565 361 345 93 66 27
PasI CC'CwG_GG
Cuts at: 0 937 1153 1326 1351 2457
Size: 937 216 173 25 1106
Fragments arranged by size:
1106 937 216 173 25
PciI A'CATG_T
Cuts at: 0 2081 2457
Size: 2081 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 657 922 2457
Size: 657 265 1535
Fragments arranged by size:
1535 657 265
PfoI T'CCnGG_A
Cuts at: 0 278 2457
Size: 278 2179
PmlI CAC'GTG
Cuts at: 0 1707 2457
Size: 1707 750
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 668 700 2457
Size: 668 32 1757
Fragments arranged by size:
1757 668 32
PpuMI rG'GwC_Cy
Cuts at: 0 276 2457
Size: 276 2181
PspOMI G'GGCC_C
Cuts at: 0 219 1119 1322 2457
Size: 219 900 203 1135
Fragments arranged by size:
1135 900 219 203
PstI C_TGCA'G
Cuts at: 0 1211 1337 2272 2372 2457
Size: 1211 126 935 100 85
Fragments arranged by size:
1211 935 126 100 85
PvuII CAG'CTG
Cuts at: 0 1099 1751 2457
Size: 1099 652 706
Fragments arranged by size:
1099 706 652
SacI G_AGCT'C
Cuts at: 0 1199 1790 2457
Size: 1199 591 667
Fragments arranged by size:
1199 667 591
SapI GCTCTTCn'nnn_
Cuts at: 0 1639 1795 2257 2457
Size: 1639 156 462 200
Fragments arranged by size:
1639 462 200 156
SexAI A'CCwGG_T
Cuts at: 0 843 2457
Size: 843 1614
SfcI C'TryA_G
Cuts at: 0 1207 1333 2178 2268 2368 2457
Size: 1207 126 845 90 100 89
Fragments arranged by size:
1207 845 126 100 90 89
SfoI GGC'GCC
Cuts at: 0 1437 2457
Size: 1437 1020
SmaI CCC'GGG
Cuts at: 0 211 224 1227 2457
Size: 211 13 1003 1230
Fragments arranged by size:
1230 1003 211 13
SmlI C'TyrA_G
Cuts at: 0 1909 1994 2457
Size: 1909 85 463
Fragments arranged by size:
1909 463 85
SrfI GCCC'GGGC
Cuts at: 0 224 2457
Size: 224 2233
StuI AGG'CCT
Cuts at: 0 205 876 1613 2457
Size: 205 671 737 844
Fragments arranged by size:
844 737 671 205
StyI C'CwwG_G
Cuts at: 0 14 71 743 997 1065 1652 2228 2457
Size: 14 57 672 254 68 587 576 229
Fragments arranged by size:
672 587 576 254 229 68 57 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 254 271 774 2457
Size: 254 17 503 1683
Fragments arranged by size:
1683 503 254 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1074 1106 2457
Size: 1074 32 1351
Fragments arranged by size:
1351 1074 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 21 378 706 2457
Size: 21 357 328 1751
Fragments arranged by size:
1751 357 328 21
XhoI C'TCGA_G
Cuts at: 0 1994 2457
Size: 1994 463
XmaI C'CCGG_G
Cuts at: 0 209 222 1225 2457
Size: 209 13 1003 1232
Fragments arranged by size:
1232 1003 209 13
XmnI GAAnn'nnTTC
Cuts at: 0 2404 2457
Size: 2404 53
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BciVI BglI
BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HindIII KasI MscI MslI
MspA1I NarI NcoI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SapI
SexAI SfcI SfoI SmaI SmlI SrfI StuI StyI
TaqII TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI
BcgI BclI BfrBI BmrI BmtI BsiWI BspEI BsrDI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI
DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TatI Tth111I XbaI ZraI