Restriction map for
pFN21AE3537
(Linear) (Six-base) MAPSORT of: KIEE3537.seq Check: 9983 from: 1 to: 1518
KIEE3537.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 210 1518
Size: 210 1308
AccI GT'mk_AC
Cuts at: 0 934 1518
Size: 934 584
AlwNI CAG_nnn'CTG
Cuts at: 0 657 1518
Size: 657 861
ApaLI G'TGCA_C
Cuts at: 0 10 1518
Size: 10 1508
ApoI r'AATT_y
Cuts at: 0 70 339 1518
Size: 70 269 1179
Fragments arranged by size:
1179 269 70
AseI AT'TA_AT
Cuts at: 0 869 1518
Size: 869 649
AvaI C'yCGr_G
Cuts at: 0 695 979 1382 1518
Size: 695 284 403 136
Fragments arranged by size:
695 403 284 136
AvrII C'CTAG_G
Cuts at: 0 450 1518
Size: 450 1068
BamHI G'GATC_C
Cuts at: 0 1210 1518
Size: 1210 308
BanI G'GyrC_C
Cuts at: 0 633 1430 1518
Size: 633 797 88
Fragments arranged by size:
797 633 88
BanII G_rGCy'C
Cuts at: 0 617 680 995 1040 1461 1518
Size: 617 63 315 45 421 57
Fragments arranged by size:
617 421 315 63 57 45
BbeI G_GCGC'C
Cuts at: 0 1434 1518
Size: 1434 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 197 553 1518
Size: 197 356 965
Fragments arranged by size:
965 356 197
BbvCI CC'TCA_GC
Cuts at: 0 1475 1518
Size: 1475 43
BlpI GC'TnA_GC
Cuts at: 0 1063 1518
Size: 1063 455
Bme1580I G_kGCm'C
Cuts at: 0 14 638 1169 1518
Size: 14 624 531 349
Fragments arranged by size:
624 531 349 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 1131 1242 1399 1518
Size: 1131 111 157 119
Fragments arranged by size:
1131 157 119 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1475 1518
Size: 1475 43
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1092 1518
Size: 199 893 426
Fragments arranged by size:
893 426 199
Bpu10I CC'TnA_GC
Cuts at: 0 1475 1518
Size: 1475 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 1445 1518
Size: 736 709 73
Fragments arranged by size:
736 709 73
BsaAI yAC'GTr
Cuts at: 0 630 1518
Size: 630 888
BsaHI Gr'CG_yC
Cuts at: 0 1431 1518
Size: 1431 87
BsaWI w'CCGG_w
Cuts at: 0 335 1518
Size: 335 1183
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1414 1518
Size: 1414 104
BseYI C'CCAG_C
Cuts at: 0 793 1301 1518
Size: 793 508 217
Fragments arranged by size:
793 508 217
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1518
Size: 586 932
BsiHKAI G_wGCw'C
Cuts at: 0 14 680 995 1461 1518
Size: 14 666 315 466 57
Fragments arranged by size:
666 466 315 57 14
BsmI GAATG_Cn'
Cuts at: 0 1216 1518
Size: 1216 302
Bsp1286I G_dGCh'C
Cuts at: 0 14 617 638 680 995 1040 1169 1461
Size: 14 603 21 42 315 45 129 292
Cuts at: 1461 1518
Size: 57
Fragments arranged by size:
603 315 292 129 57 45 42 21
14
BspHI T'CATG_A
Cuts at: 0 44 1518
Size: 44 1474
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 210 608 1518
Size: 210 398 910
Fragments arranged by size:
910 398 210
BssSI C'ACGA_G
Cuts at: 0 30 1518
Size: 30 1488
BstEII G'GTnAC_C
Cuts at: 0 222 807 1518
Size: 222 585 711
Fragments arranged by size:
711 585 222
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 1518
Size: 282 1236
BstYI r'GATC_y
Cuts at: 0 1210 1518
Size: 1210 308
Bsu36I CC'TnA_GG
Cuts at: 0 1111 1161 1518
Size: 1111 50 357
Fragments arranged by size:
1111 357 50
BtgI C'CryG_G
Cuts at: 0 62 950 1518
Size: 62 888 568
Fragments arranged by size:
888 568 62
DraIII CAC_nnn'GTG
Cuts at: 0 726 1518
Size: 726 792
DrdI GACnn_nn'nnGTC
Cuts at: 0 1509 1518
Size: 1509 9
EaeI y'GGCC_r
Cuts at: 0 668 1016 1518
Size: 668 348 502
Fragments arranged by size:
668 502 348
EarI CTCTTCn'nnn_
Cuts at: 0 364 685 1089 1518
Size: 364 321 404 429
Fragments arranged by size:
429 404 364 321
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 317 1518
Size: 317 1201
EcoICRI GAG'CTC
Cuts at: 0 678 993 1459 1518
Size: 678 315 466 59
Fragments arranged by size:
678 466 315 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 1092 1518
Size: 199 893 426
Fragments arranged by size:
893 426 199
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 217 1518
Size: 136 81 1301
Fragments arranged by size:
1301 136 81
EcoRV GAT'ATC
Cuts at: 0 186 408 1518
Size: 186 222 1110
Fragments arranged by size:
1110 222 186
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 148 1518
Size: 148 1370
FspI TGC'GCA
Cuts at: 0 858 967 1518
Size: 858 109 551
Fragments arranged by size:
858 551 109
FspAI rTGC'GCAy
Cuts at: 0 967 1518
Size: 967 551
HaeII r_GCGC'y
Cuts at: 0 1434 1518
Size: 1434 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1491 1518
Size: 1491 27
HincII GTy'rAC
Cuts at: 0 516 935 1518
Size: 516 419 583
Fragments arranged by size:
583 516 419
KasI G'GCGC_C
Cuts at: 0 1430 1518
Size: 1430 88
MfeI C'AATT_G
Cuts at: 0 774 1518
Size: 774 744
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 1518
Size: 461 1057
MscI TGG'CCA
Cuts at: 0 670 1018 1518
Size: 670 348 500
Fragments arranged by size:
670 500 348
MslI CAynn'nnrTG
Cuts at: 0 280 1518
Size: 280 1238
MspA1I CmG'CkG
Cuts at: 0 413 1518
Size: 413 1105
NarI GG'CG_CC
Cuts at: 0 1431 1518
Size: 1431 87
NcoI C'CATG_G
Cuts at: 0 62 950 1518
Size: 62 888 568
Fragments arranged by size:
888 568 62
NdeI CA'TA_TG
Cuts at: 0 90 1518
Size: 90 1428
NspI r_CATG'y
Cuts at: 0 543 1518
Size: 543 975
PasI CC'CwG_GG
Cuts at: 0 1040 1260 1518
Size: 1040 220 258
Fragments arranged by size:
1040 258 220
PflMI CCAn_nnn'nTGG
Cuts at: 0 158 397 623 646 1518
Size: 158 239 226 23 872
Fragments arranged by size:
872 239 226 158 23
SacI G_AGCT'C
Cuts at: 0 680 995 1461 1518
Size: 680 315 466 57
Fragments arranged by size:
680 466 315 57
SalI G'TCGA_C
Cuts at: 0 933 1518
Size: 933 585
SapI GCTCTTCn'nnn_
Cuts at: 0 364 685 1089 1518
Size: 364 321 404 429
Fragments arranged by size:
429 404 364 321
ScaI AGT'ACT
Cuts at: 0 1088 1518
Size: 1088 430
SfcI C'TryA_G
Cuts at: 0 743 1518
Size: 743 775
SfoI GGC'GCC
Cuts at: 0 1432 1518
Size: 1432 86
SmlI C'TyrA_G
Cuts at: 0 751 1382 1460 1518
Size: 751 631 78 58
Fragments arranged by size:
751 631 78 58
StuI AGG'CCT
Cuts at: 0 709 1473 1518
Size: 709 764 45
Fragments arranged by size:
764 709 45
StyI C'CwwG_G
Cuts at: 0 62 111 137 152 450 950 1518
Size: 62 49 26 15 298 500 568
Fragments arranged by size:
568 500 298 62 49 26 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 1518
Size: 165 1353
TatI w'GTAC_w
Cuts at: 0 530 1086 1378 1518
Size: 530 556 292 140
Fragments arranged by size:
556 530 292 140
Tth111I GACn'n_nGTC
Cuts at: 0 1248 1518
Size: 1248 270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 155 1518
Size: 155 1363
XhoI C'TCGA_G
Cuts at: 0 1382 1518
Size: 1382 136
Enzymes that do cut:
AarI AccI AlwNI ApaLI ApoI AseI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BssSI BstEII BstXI BstYI Bsu36I BtgI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoRV FalI
FspI FspAI HaeII Hin4I HincII KasI MfeI MmeI
MscI MslI MspA1I NarI NcoI NdeI NspI PasI
PflMI SacI SalI SapI ScaI SfcI SfoI SmlI
StuI StyI TaqII TatI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AsiSI BaeI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BsaI BsaBI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI
BtsI ClaI CspCI DraI EagI EcoO109I EcoRI FseI
HindIII HpaI KpnI MluI NaeI NgoMIV NheI NotI
NruI NsiI PacI PciI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SanDI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XmaI XmnI ZraI