Restriction map for
pFN21AE3534
(Linear) (Six-base) MAPSORT of: KIEE3534.seq Check: 7386 from: 1 to: 1923
KIEE3534.orf
With 182 enzymes: SgfI *
July 3, 2012 18:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 501 1923
Size: 501 1422
AccI GT'mk_AC
Cuts at: 0 208 1076 1923
Size: 208 868 847
Fragments arranged by size:
868 847 208
AclI AA'CG_TT
Cuts at: 0 977 1923
Size: 977 946
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 1636 1923
Size: 1113 523 287
Fragments arranged by size:
1113 523 287
AfeI AGC'GCT
Cuts at: 0 155 1923
Size: 155 1768
AhdI GACnn_n'nnGTC
Cuts at: 0 1857 1923
Size: 1857 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1025 1793 1923
Size: 1025 768 130
Fragments arranged by size:
1025 768 130
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 754 786 1923
Size: 754 32 1137
Fragments arranged by size:
1137 754 32
AlwNI CAG_nnn'CTG
Cuts at: 0 171 1923
Size: 171 1752
ApaI G_GGCC'C
Cuts at: 0 305 489 1392 1620 1923
Size: 305 184 903 228 303
Fragments arranged by size:
903 305 303 228 184
ApaLI G'TGCA_C
Cuts at: 0 447 1923
Size: 447 1476
AscI GG'CGCG_CC
Cuts at: 0 358 1923
Size: 358 1565
AvaI C'yCGr_G
Cuts at: 0 1085 1199 1364 1923
Size: 1085 114 165 559
Fragments arranged by size:
1085 559 165 114
BamHI G'GATC_C
Cuts at: 0 1410 1923
Size: 1410 513
BanI G'GyrC_C
Cuts at: 0 50 366 402 659 1923
Size: 50 316 36 257 1264
Fragments arranged by size:
1264 316 257 50 36
BanII G_rGCy'C
Cuts at: 0 11 305 489 1092 1392 1620 1923
Size: 11 294 184 603 300 228 303
Fragments arranged by size:
603 303 300 294 228 184 11
BbeI G_GCGC'C
Cuts at: 0 54 663 1923
Size: 54 609 1260
Fragments arranged by size:
1260 609 54
BbvCI CC'TCA_GC
Cuts at: 0 157 1923
Size: 157 1766
BciVI GTATCCnnnnn_n'
Cuts at: 0 150 1923
Size: 150 1773
BglI GCCn_nnn'nGGC
Cuts at: 0 33 1923
Size: 33 1890
Bme1580I G_kGCm'C
Cuts at: 0 305 371 407 451 489 1392 1560 1620
Size: 305 66 36 44 38 903 168 60
Cuts at: 1620 1923
Size: 303
Fragments arranged by size:
903 305 303 168 66 60 44 38
36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1614 1923
Size: 1614 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1199 1616 1923
Size: 337 862 417 307
Fragments arranged by size:
862 417 337 307
Bpu10I CC'TnA_GC
Cuts at: 0 157 1923
Size: 157 1766
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 1923
Size: 120 1803
BsaI GGTCTCn'nnnn_
Cuts at: 0 424 811 1923
Size: 424 387 1112
Fragments arranged by size:
1112 424 387
BsaAI yAC'GTr
Cuts at: 0 1265 1923
Size: 1265 658
BsaHI Gr'CG_yC
Cuts at: 0 51 499 660 788 1923
Size: 51 448 161 128 1135
Fragments arranged by size:
1135 448 161 128 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 754 784 1589 1619 1923
Size: 754 30 805 30 304
Fragments arranged by size:
805 754 304 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 883 1923
Size: 775 108 1040
Fragments arranged by size:
1040 775 108
BseYI C'CCAG_C
Cuts at: 0 65 586 1391 1586 1766 1923
Size: 65 521 805 195 180 157
Fragments arranged by size:
805 521 195 180 157 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 787 1231 1339 1923
Size: 41 746 444 108 584
Fragments arranged by size:
746 584 444 108 41
BsiEI CG_ry'CG
Cuts at: 0 46 215 260 470 837 1923
Size: 46 169 45 210 367 1086
Fragments arranged by size:
1086 367 210 169 46 45
BsiHKAI G_wGCw'C
Cuts at: 0 451 1923
Size: 451 1472
BsmI GAATG_Cn'
Cuts at: 0 1228 1395 1923
Size: 1228 167 528
Fragments arranged by size:
1228 528 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 895 1923
Size: 895 1028
Bsp1286I G_dGCh'C
Cuts at: 0 11 305 371 407 451 489 1092 1392
Size: 11 294 66 36 44 38 603 300
Cuts at: 1392 1560 1620 1923
Size: 168 60 303
Fragments arranged by size:
603 303 300 294 168 66 60 44
38 36 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 501 1838 1923
Size: 501 1337 85
Fragments arranged by size:
1337 501 85
BsrBI CCG'CTC
Cuts at: 0 604 793 1923
Size: 604 189 1130
Fragments arranged by size:
1130 604 189
BsrDI GCAATG_nn'
Cuts at: 0 1334 1923
Size: 1334 589
BsrFI r'CCGG_y
Cuts at: 0 53 414 534 593 656 874 1152 1923
Size: 53 361 120 59 63 218 278 771
Fragments arranged by size:
771 361 278 218 120 63 59 53
BssHII G'CGCG_C
Cuts at: 0 334 358 388 839 1923
Size: 334 24 30 451 1084
Fragments arranged by size:
1084 451 334 30 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 171 1167 1923
Size: 171 996 756
Fragments arranged by size:
996 756 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1586 1752 1923
Size: 1586 166 171
Fragments arranged by size:
1586 171 166
BstYI r'GATC_y
Cuts at: 0 1410 1923
Size: 1410 513
BstZ17I GTA'TAC
Cuts at: 0 1077 1923
Size: 1077 846
BtgI C'CryG_G
Cuts at: 0 1781 1923
Size: 1781 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 612 1923
Size: 612 1311
BtsI GCAGTG_nn'
Cuts at: 0 1322 1923
Size: 1322 601
DraIII CAC_nnn'GTG
Cuts at: 0 1564 1923
Size: 1564 359
EaeI y'GGCC_r
Cuts at: 0 43 212 257 291 467 566 774 834
Size: 43 169 45 34 176 99 208 60
Cuts at: 834 1490 1923
Size: 656 433
Fragments arranged by size:
656 433 208 176 169 99 60 45
43 34
EagI C'GGCC_G
Cuts at: 0 43 212 257 467 834 1923
Size: 43 169 45 210 367 1089
Fragments arranged by size:
1089 367 210 169 45 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1113 1199 1616 1636 1923
Size: 337 776 86 417 20 287
Fragments arranged by size:
776 417 337 287 86 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 868 1923
Size: 397 471 1055
Fragments arranged by size:
1055 471 397
EcoO109I rG'GnC_Cy
Cuts at: 0 301 485 486 1388 1616 1827 1923
Size: 301 184 1 902 228 211 96
Fragments arranged by size:
902 301 228 211 184 96 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1442 1923
Size: 1442 481
FspI TGC'GCA
Cuts at: 0 754 1923
Size: 754 1169
HaeII r_GCGC'y
Cuts at: 0 54 157 663 1923
Size: 54 103 506 1260
Fragments arranged by size:
1260 506 103 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1843 1875 1923
Size: 1843 32 48
Fragments arranged by size:
1843 48 32
HincII GTy'rAC
Cuts at: 0 209 1625 1923
Size: 209 1416 298
Fragments arranged by size:
1416 298 209
KasI G'GCGC_C
Cuts at: 0 50 659 1923
Size: 50 609 1264
Fragments arranged by size:
1264 609 50
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 998 1923
Size: 998 925
MslI CAynn'nnrTG
Cuts at: 0 1903 1923
Size: 1903 20
MspA1I CmG'CkG
Cuts at: 0 77 126 552 733 1050 1857 1923
Size: 77 49 426 181 317 807 66
Fragments arranged by size:
807 426 317 181 77 66 49
NaeI GCC'GGC
Cuts at: 0 55 536 595 876 1923
Size: 55 481 59 281 1047
Fragments arranged by size:
1047 481 281 59 55
NarI GG'CG_CC
Cuts at: 0 51 660 1923
Size: 51 609 1263
Fragments arranged by size:
1263 609 51
NcoI C'CATG_G
Cuts at: 0 1781 1923
Size: 1781 142
NdeI CA'TA_TG
Cuts at: 0 1646 1923
Size: 1646 277
NgoMIV G'CCGG_C
Cuts at: 0 53 534 593 874 1923
Size: 53 481 59 281 1049
Fragments arranged by size:
1049 481 281 59 53
PflMI CCAn_nnn'nTGG
Cuts at: 0 783 1923
Size: 783 1140
PfoI T'CCnGG_A
Cuts at: 0 106 263 1923
Size: 106 157 1660
Fragments arranged by size:
1660 157 106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 754 786 1923
Size: 754 32 1137
Fragments arranged by size:
1137 754 32
PpuMI rG'GwC_Cy
Cuts at: 0 1827 1923
Size: 1827 96
PspOMI G'GGCC_C
Cuts at: 0 301 485 1388 1616 1923
Size: 301 184 903 228 307
Fragments arranged by size:
903 307 301 228 184
PstI C_TGCA'G
Cuts at: 0 347 1049 1163 1923
Size: 347 702 114 760
Fragments arranged by size:
760 702 347 114
PvuII CAG'CTG
Cuts at: 0 126 1050 1857 1923
Size: 126 924 807 66
Fragments arranged by size:
924 807 126 66
RsrII CG'GwC_CG
Cuts at: 0 417 1923
Size: 417 1506
SalI G'TCGA_C
Cuts at: 0 207 1923
Size: 207 1716
SfcI C'TryA_G
Cuts at: 0 343 1045 1159 1923
Size: 343 702 114 764
Fragments arranged by size:
764 702 343 114
SfoI GGC'GCC
Cuts at: 0 52 661 1923
Size: 52 609 1262
Fragments arranged by size:
1262 609 52
SgrAI Cr'CCGG_yG
Cuts at: 0 53 1923
Size: 53 1870
SmlI C'TyrA_G
Cuts at: 0 99 1923
Size: 99 1824
StuI AGG'CCT
Cuts at: 0 322 1036 1923
Size: 322 714 887
Fragments arranged by size:
887 714 322
StyI C'CwwG_G
Cuts at: 0 1781 1862 1923
Size: 1781 81 61
Fragments arranged by size:
1781 81 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 72 1666 1923
Size: 72 1594 257
Fragments arranged by size:
1594 257 72
Tth111I GACn'n_nGTC
Cuts at: 0 351 498 1923
Size: 351 147 1425
Fragments arranged by size:
1425 351 147
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1308 1923
Size: 1308 615
Enzymes that do cut:
AarI AccI AclI AcuI AfeI AhdI AlfI AloI
AlwNI ApaI ApaLI AscI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstXI BstYI BstZ17I BtgI BtgZI
BtsI DraIII EaeI EagI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII KasI MmeI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV PflMI PfoI PpiI
PpuMI PspOMI PstI PvuII RsrII SalI SfcI SfoI
SgrAI SmlI StuI StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AflII AflIII AgeI AleI ApoI AseI
AsiSI AvrII BaeI BbsI BcgI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BsaBI BsaWI BsiWI BspEI
BspHI BsrGI BssSI BstBI BstEII Bsu36I ClaI CspCI
DraI DrdI EarI EciI EcoICRI EcoRI EcoRV FseI
FspAI HindIII HpaI KpnI MfeI MluI MscI NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI SacI SacII
SanDI SapI SbfI ScaI SexAI SfiI SgfI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
XbaI XhoI XmaI XmnI ZraI