Restriction map for
pFN21AE3530
(Linear) (Six-base) MAPSORT of: KIEE3530.seq Check: 5983 from: 1 to: 3720
KIEE3530.orf
With 182 enzymes: SgfI *
July 3, 2012 18:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 813 1197 1698 2699 3693 3720
Size: 813 384 501 1001 994 27
Fragments arranged by size:
1001 994 813 501 384 27
AatII G_ACGT'C
Cuts at: 0 401 713 3539 3720
Size: 401 312 2826 181
Fragments arranged by size:
2826 401 312 181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1273 1321 3720
Size: 907 366 48 2399
Fragments arranged by size:
2399 907 366 48
AfeI AGC'GCT
Cuts at: 0 3639 3720
Size: 3639 81
AgeI A'CCGG_T
Cuts at: 0 353 3720
Size: 353 3367
AhdI GACnn_n'nnGTC
Cuts at: 0 1332 3720
Size: 1332 2388
AleI CACnn'nnGTG
Cuts at: 0 256 3080 3720
Size: 256 2824 640
Fragments arranged by size:
2824 640 256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 1669 3415 3720
Size: 274 1395 1746 305
Fragments arranged by size:
1746 1395 305 274
AlwNI CAG_nnn'CTG
Cuts at: 0 238 1428 1848 1857 1929 1974 2461 2533
Size: 238 1190 420 9 72 45 487 72
Cuts at: 2533 2628 3111 3720
Size: 95 483 609
Fragments arranged by size:
1190 609 487 483 420 238 95 72
72 45 9
ApaI G_GGCC'C
Cuts at: 0 312 480 796 1667 2286 2795 2852 3305
Size: 312 168 316 871 619 509 57 453
Cuts at: 3305 3439 3720
Size: 134 281
Fragments arranged by size:
871 619 509 453 316 312 281 168
134 57
AvaI C'yCGr_G
Cuts at: 0 430 540 1186 2258 2569 2794 2852 3305
Size: 430 110 646 1072 311 225 58 453
Cuts at: 3305 3432 3720
Size: 127 288
Fragments arranged by size:
1072 646 453 430 311 288 225 127
110 58
AvrII C'CTAG_G
Cuts at: 0 498 3720
Size: 498 3222
BanI G'GyrC_C
Cuts at: 0 258 1653 1735 1774 1915 1955 2219 2617
Size: 258 1395 82 39 141 40 264 398
Cuts at: 2617 2980 3462 3705 3720
Size: 363 482 243 15
Fragments arranged by size:
1395 482 398 363 264 258 243 141
82 40 39 15
BanII G_rGCy'C
Cuts at: 0 312 415 480 511 578 746 796 817
Size: 312 103 65 31 67 168 50 21
Cuts at: 817 850 935 1667 2286 2524 2576 2647 2770
Size: 33 85 732 619 238 52 71 123
Cuts at: 2770 2795 2852 3305 3347 3439 3720
Size: 25 57 453 42 92 281
Fragments arranged by size:
732 619 453 312 281 238 168 123
103 92 85 71 67 65 57 52
50 42 33 31 25 21
BbeI G_GCGC'C
Cuts at: 0 1778 3466 3720
Size: 1778 1688 254
Fragments arranged by size:
1778 1688 254
BbsI GAAGACnn'nnnn_
Cuts at: 0 667 1560 3720
Size: 667 893 2160
Fragments arranged by size:
2160 893 667
BbvCI CC'TCA_GC
Cuts at: 0 818 2888 3296 3311 3720
Size: 818 2070 408 15 409
Fragments arranged by size:
2070 818 409 408 15
BciVI GTATCCnnnnn_n'
Cuts at: 0 887 3720
Size: 887 2833
BglI GCCn_nnn'nGGC
Cuts at: 0 1662 1783 2175 2836 3238 3330 3365 3720
Size: 1662 121 392 661 402 92 35 355
Fragments arranged by size:
1662 661 402 392 355 121 92 35
BlpI GC'TnA_GC
Cuts at: 0 943 3720
Size: 943 2777
Bme1580I G_kGCm'C
Cuts at: 0 312 480 796 1658 1667 2224 2265 2286
Size: 312 168 316 862 9 557 41 21
Cuts at: 2286 2795 2852 3127 3218 3305 3439 3710 3720
Size: 509 57 275 91 87 134 271 10
Fragments arranged by size:
862 557 509 316 312 275 271 168
134 91 87 57 41 21 10 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 2616 3720
Size: 2616 1104
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2019 3522 3606 3720
Size: 2019 1503 84 114
Fragments arranged by size:
2019 1503 114 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 495 525 676 769 883 1381 1657
Size: 303 192 30 151 93 114 498 276
Cuts at: 1657 2512 2560 2782 2842 3034 3720
Size: 855 48 222 60 192 686
Fragments arranged by size:
855 686 498 303 276 222 192 192
151 114 93 60 48 30
Bpu10I CC'TnA_GC
Cuts at: 0 811 818 2104 2888 3296 3311 3720
Size: 811 7 1286 784 408 15 409
Fragments arranged by size:
1286 811 784 409 408 15 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1931 3720
Size: 1180 751 1789
Fragments arranged by size:
1789 1180 751
BsaI GGTCTCn'nnnn_
Cuts at: 0 1510 3001 3207 3720
Size: 1510 1491 206 513
Fragments arranged by size:
1510 1491 513 206
BsaAI yAC'GTr
Cuts at: 0 876 1122 3363 3720
Size: 876 246 2241 357
Fragments arranged by size:
2241 876 357 246
BsaBI GATnn'nnATC
Cuts at: 0 422 3034 3720
Size: 422 2612 686
Fragments arranged by size:
2612 686 422
BsaHI Gr'CG_yC
Cuts at: 0 152 398 710 1775 2957 3463 3536 3569
Size: 152 246 312 1065 1182 506 73 33
Cuts at: 3569 3720
Size: 151
Fragments arranged by size:
1182 1065 506 312 246 152 151 73
33
BsaWI w'CCGG_w
Cuts at: 0 353 830 1323 3720
Size: 353 477 493 2397
Fragments arranged by size:
2397 493 477 353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 498 528 985 1015 1507 1537
Size: 190 30 278 30 457 30 492 30
Cuts at: 1537 2634 2664 3720
Size: 1097 30 1056
Fragments arranged by size:
1097 1056 492 457 278 190 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 8 60 562 742 745 2173 2244 2509
Size: 8 52 502 180 3 1428 71 265
Cuts at: 2509 2512 2584 2964 3304 3328 3511 3682 3720
Size: 3 72 380 340 24 183 171 38
Fragments arranged by size:
1428 502 380 340 265 183 180 171
72 71 52 38 24 8 3 3
BseYI C'CCAG_C
Cuts at: 0 157 231 472 614 777 789 2316 3720
Size: 157 74 241 142 163 12 1527 1404
Fragments arranged by size:
1527 1404 241 163 157 142 74 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 877 976 1233 1726 1894 3720
Size: 682 195 99 257 493 168 1826
Fragments arranged by size:
1826 682 493 257 195 168 99
BsiEI CG_ry'CG
Cuts at: 0 2061 3720
Size: 2061 1659
BsiHKAI G_wGCw'C
Cuts at: 0 511 1760 2468 2666 3491 3720
Size: 511 1249 708 198 825 229
Fragments arranged by size:
1249 825 708 511 229 198
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1357 1498 2026 2507 3670 3720
Size: 1357 141 528 481 1163 50
Fragments arranged by size:
1357 1163 528 481 141 50
Bsp1286I G_dGCh'C
Cuts at: 0 312 415 480 511 578 746 796 817
Size: 312 103 65 31 67 168 50 21
Cuts at: 817 850 935 1658 1667 1760 2224 2265 2286
Size: 33 85 723 9 93 464 41 21
Cuts at: 2286 2468 2524 2576 2647 2666 2770 2795 2852
Size: 182 56 52 71 19 104 25 57
Cuts at: 2852 3127 3218 3305 3347 3439 3491 3710 3720
Size: 275 91 87 42 92 52 219 10
Fragments arranged by size:
723 464 312 275 219 182 168 104
103 93 92 91 87 85 71 67
65 57 56 52 52 50 42 41
33 31 25 21 21 19 10 9
BspEI T'CCGG_A
Cuts at: 0 830 1323 3720
Size: 830 493 2397
Fragments arranged by size:
2397 830 493
BspHI T'CATG_A
Cuts at: 0 620 3720
Size: 620 3100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 455 813 1197 1698 1865 2699 3693 3720
Size: 455 358 384 501 167 834 994 27
Fragments arranged by size:
994 834 501 455 384 358 167 27
BsrBI CCG'CTC
Cuts at: 0 638 3720
Size: 638 3082
BsrDI GCAATG_nn'
Cuts at: 0 282 3720
Size: 282 3438
BsrFI r'CCGG_y
Cuts at: 0 353 2417 3459 3551 3720
Size: 353 2064 1042 92 169
Fragments arranged by size:
2064 1042 353 169 92
BsrGI T'GTAC_A
Cuts at: 0 1109 3720
Size: 1109 2611
BssSI C'ACGA_G
Cuts at: 0 3340 3720
Size: 3340 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1857 2624 2661 3720
Size: 1857 767 37 1059
Fragments arranged by size:
1857 1059 767 37
BstEII G'GTnAC_C
Cuts at: 0 1528 3720
Size: 1528 2192
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1095 1674 1732 3258 3720
Size: 1095 579 58 1526 462
Fragments arranged by size:
1526 1095 579 462 58
BstYI r'GATC_y
Cuts at: 0 986 1418 1997 3720
Size: 986 432 579 1723
Fragments arranged by size:
1723 986 579 432
Bsu36I CC'TnA_GG
Cuts at: 0 1476 1794 3720
Size: 1476 318 1926
Fragments arranged by size:
1926 1476 318
BtgI C'CryG_G
Cuts at: 0 1094 1533 1874 2546 3720
Size: 1094 439 341 672 1174
Fragments arranged by size:
1174 1094 672 439 341
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3021 3720
Size: 3021 699
BtsI GCAGTG_nn'
Cuts at: 0 993 2610 2927 3657 3720
Size: 993 1617 317 730 63
Fragments arranged by size:
1617 993 730 317 63
DraIII CAC_nnn'GTG
Cuts at: 0 591 2624 2940 3720
Size: 591 2033 316 780
Fragments arranged by size:
2033 780 591 316
DrdI GACnn_nn'nnGTC
Cuts at: 0 1588 3136 3535 3720
Size: 1588 1548 399 185
Fragments arranged by size:
1588 1548 399 185
EaeI y'GGCC_r
Cuts at: 0 671 1871 2006 2058 2138 2543 3521 3596
Size: 671 1200 135 52 80 405 978 75
Cuts at: 3596 3720
Size: 124
Fragments arranged by size:
1200 978 671 405 135 124 80 75
52
EagI C'GGCC_G
Cuts at: 0 2058 3720
Size: 2058 1662
EarI CTCTTCn'nnn_
Cuts at: 0 122 328 371 1075 1843 1988 2194 3001
Size: 122 206 43 704 768 145 206 807
Cuts at: 3001 3720
Size: 719
Fragments arranged by size:
807 768 719 704 206 206 145 122
43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 505 2387 2518 2980 3720
Size: 505 1882 131 462 740
Fragments arranged by size:
1882 740 505 462 131
EcoICRI GAG'CTC
Cuts at: 0 509 3720
Size: 509 3211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 495 525 676 769 883 907 1273
Size: 303 192 30 151 93 114 24 366
Cuts at: 1273 1321 1381 1657 2512 2560 2782 2842 3034
Size: 48 60 276 855 48 222 60 192
Cuts at: 3034 3720
Size: 686
Fragments arranged by size:
855 686 366 303 276 222 192 192
151 114 93 60 60 48 48 30
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 32 376 1288 1840 1861 2347 3720
Size: 32 344 912 552 21 486 1373
Fragments arranged by size:
1373 912 552 486 344 32 21
EcoO109I rG'GnC_Cy
Cuts at: 0 64 308 476 477 495 793 807 1065
Size: 64 244 168 1 18 298 14 258
Cuts at: 1065 1663 1854 1866 2282 2283 2714 2757 2791
Size: 598 191 12 416 1 431 43 34
Cuts at: 2791 2849 3301 3302 3663 3720
Size: 58 452 1 361 57
Fragments arranged by size:
598 452 431 416 361 298 258 244
191 168 64 58 57 43 34 18
14 12 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1197 3720
Size: 1197 2523
FspI TGC'GCA
Cuts at: 0 422 464 682 3547 3720
Size: 422 42 218 2865 173
Fragments arranged by size:
2865 422 218 173 42
FspAI rTGC'GCAy
Cuts at: 0 422 464 3720
Size: 422 42 3256
Fragments arranged by size:
3256 422 42
HaeII r_GCGC'y
Cuts at: 0 943 950 1696 1778 3466 3641 3720
Size: 943 7 746 82 1688 175 79
Fragments arranged by size:
1688 943 746 175 82 79 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 327 359 1318 1350 1929 1961 2634 2666
Size: 327 32 959 32 579 32 673 32
Cuts at: 2666 3720
Size: 1054
Fragments arranged by size:
1054 959 673 579 327 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1472 1511 2124 2304 3720
Size: 1472 39 613 180 1416
Fragments arranged by size:
1472 1416 613 180 39
HindIII A'AGCT_T
Cuts at: 0 2895 3720
Size: 2895 825
KasI G'GCGC_C
Cuts at: 0 1774 3462 3720
Size: 1774 1688 258
Fragments arranged by size:
1774 1688 258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 2581 3720
Size: 35 2546 1139
Fragments arranged by size:
2546 1139 35
MscI TGG'CCA
Cuts at: 0 2140 3598 3720
Size: 2140 1458 122
Fragments arranged by size:
2140 1458 122
MslI CAynn'nnrTG
Cuts at: 0 256 1939 2217 3080 3720
Size: 256 1683 278 863 640
Fragments arranged by size:
1683 863 640 278 256
MspA1I CmG'CkG
Cuts at: 0 45 238 289 323 1344 1428 1485 1491
Size: 45 193 51 34 1021 84 57 6
Cuts at: 1491 1535 1635 2741 2843 2954 3441 3720
Size: 44 100 1106 102 111 487 279
Fragments arranged by size:
1106 1021 487 279 193 111 102 100
84 57 51 45 44 34 6
NaeI GCC'GGC
Cuts at: 0 3461 3553 3720
Size: 3461 92 167
Fragments arranged by size:
3461 167 92
NarI GG'CG_CC
Cuts at: 0 1775 3463 3720
Size: 1775 1688 257
Fragments arranged by size:
1775 1688 257
NcoI C'CATG_G
Cuts at: 0 1094 3720
Size: 1094 2626
NgoMIV G'CCGG_C
Cuts at: 0 3459 3551 3720
Size: 3459 92 169
Fragments arranged by size:
3459 169 92
NspI r_CATG'y
Cuts at: 0 1299 1587 3720
Size: 1299 288 2133
Fragments arranged by size:
2133 1299 288
PasI CC'CwG_GG
Cuts at: 0 147 1313 1659 3720
Size: 147 1166 346 2061
Fragments arranged by size:
2061 1166 346 147
PflMI CCAn_nnn'nTGG
Cuts at: 0 2628 3720
Size: 2628 1092
PfoI T'CCnGG_A
Cuts at: 0 51 746 899 1841 2153 2483 2576 2864
Size: 51 695 153 942 312 330 93 288
Cuts at: 2864 3720
Size: 856
Fragments arranged by size:
942 856 695 330 312 288 153 93
51
PmlI CAC'GTG
Cuts at: 0 3363 3720
Size: 3363 357
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2407 2439 3720
Size: 2407 32 1281
Fragments arranged by size:
2407 1281 32
PpuMI rG'GwC_Cy
Cuts at: 0 495 1065 1854 1866 2714 2757 3720
Size: 495 570 789 12 848 43 963
Fragments arranged by size:
963 848 789 570 495 43 12
PspOMI G'GGCC_C
Cuts at: 0 308 476 792 1663 2282 2791 2848 3301
Size: 308 168 316 871 619 509 57 453
Cuts at: 3301 3435 3720
Size: 134 285
Fragments arranged by size:
871 619 509 453 316 308 285 168
134 57
PspXI vC'TCGA_Gb
Cuts at: 0 1186 3720
Size: 1186 2534
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 436 468 3720
Size: 436 32 3252
Fragments arranged by size:
3252 436 32
PstI C_TGCA'G
Cuts at: 0 1343 1853 1862 1970 2276 2641 2657 2708
Size: 1343 510 9 108 306 365 16 51
Cuts at: 2708 2885 3720
Size: 177 835
Fragments arranged by size:
1343 835 510 365 306 177 108 51
16 9
PvuII CAG'CTG
Cuts at: 0 45 238 323 1344 1428 1491 1635 2843
Size: 45 193 85 1021 84 63 144 1208
Cuts at: 2843 3720
Size: 877
Fragments arranged by size:
1208 1021 877 193 144 85 84 63
45
SacI G_AGCT'C
Cuts at: 0 511 3720
Size: 511 3209
SacII CC_GC'GG
Cuts at: 0 1536 3720
Size: 1536 2184
SanDI GG'GwC_CC
Cuts at: 0 2714 3720
Size: 2714 1006
SapI GCTCTTCn'nnn_
Cuts at: 0 2194 3720
Size: 2194 1526
SbfI CC_TGCA'GG
Cuts at: 0 2708 3720
Size: 2708 1012
SexAI A'CCwGG_T
Cuts at: 0 1406 3720
Size: 1406 2314
SfcI C'TryA_G
Cuts at: 0 528 1339 1849 1858 1966 2272 2598 2637
Size: 528 811 510 9 108 306 326 39
Cuts at: 2637 2653 2704 2881 3720
Size: 16 51 177 839
Fragments arranged by size:
839 811 528 510 326 306 177 108
51 39 16 9
SfoI GGC'GCC
Cuts at: 0 1776 3464 3720
Size: 1776 1688 256
Fragments arranged by size:
1776 1688 256
SmaI CCC'GGG
Cuts at: 0 432 2260 2796 2854 3307 3434 3720
Size: 432 1828 536 58 453 127 286
Fragments arranged by size:
1828 536 453 432 286 127 58
SmlI C'TyrA_G
Cuts at: 0 1186 1195 1946 3720
Size: 1186 9 751 1774
Fragments arranged by size:
1774 1186 751 9
SrfI GCCC'GGGC
Cuts at: 0 2796 3720
Size: 2796 924
StuI AGG'CCT
Cuts at: 0 1162 1786 3720
Size: 1162 624 1934
Fragments arranged by size:
1934 1162 624
StyI C'CwwG_G
Cuts at: 0 498 644 935 1094 1553 3720
Size: 498 146 291 159 459 2167
Fragments arranged by size:
2167 498 459 291 159 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 258 2635 3108 3531 3720
Size: 258 2377 473 423 189
Fragments arranged by size:
2377 473 423 258 189
TatI w'GTAC_w
Cuts at: 0 1109 3720
Size: 1109 2611
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 498 530 577 609 985 1017
Size: 190 32 276 32 47 32 376 32
Cuts at: 1017 1823 1855 2328 2360 2538 2570 2818 2850
Size: 806 32 473 32 178 32 248 32
Cuts at: 2850 3720
Size: 870
Fragments arranged by size:
870 806 473 376 276 248 190 178
47 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 397 1225 3720
Size: 397 828 2495
Fragments arranged by size:
2495 828 397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 651 2073 2695 3516 3720
Size: 144 507 1422 622 821 204
Fragments arranged by size:
1422 821 622 507 204 144
XhoI C'TCGA_G
Cuts at: 0 1186 3720
Size: 1186 2534
XmaI C'CCGG_G
Cuts at: 0 430 2258 2794 2852 3305 3432 3720
Size: 430 1828 536 58 453 127 288
Fragments arranged by size:
1828 536 453 430 288 127 58
XmnI GAAnn'nnTTC
Cuts at: 0 1129 3720
Size: 1129 2591
ZraI GAC'GTC
Cuts at: 0 399 711 3537 3720
Size: 399 312 2826 183
Fragments arranged by size:
2826 399 312 183
Enzymes that do cut:
AarI AatII AcuI AfeI AgeI AhdI AleI AlfI
AlwNI ApaI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BciVI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuII SacI SacII SanDI
SapI SbfI SexAI SfcI SfoI SmaI SmlI SrfI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AflIII AloI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BcgI BclI BfrBI
BglII BmgBI BmtI BsiWI BsmI BssHII BstBI BstZ17I
ClaI CspCI DraI EcoRI EcoRV FseI HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PshAI PsiI PvuI RsrII SalI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI SwaI
XbaI