Restriction map for
pFN21AE3527
(Linear) (Six-base) MAPSORT of: KIEE3527.seq Check: 701 from: 1 to: 1287
KIEE3527.orf
With 182 enzymes: SgfI *
July 3, 2012 18:05 ..
AflIII A'CryG_T
Cuts at: 0 511 1287
Size: 511 776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 509 1287
Size: 509 778
AlwNI CAG_nnn'CTG
Cuts at: 0 127 1287
Size: 127 1160
AvaI C'yCGr_G
Cuts at: 0 11 79 610 1287
Size: 11 68 531 677
Fragments arranged by size:
677 531 68 11
BamHI G'GATC_C
Cuts at: 0 1023 1287
Size: 1023 264
BanI G'GyrC_C
Cuts at: 0 75 233 480 1133 1287
Size: 75 158 247 653 154
Fragments arranged by size:
653 247 158 154 75
BanII G_rGCy'C
Cuts at: 0 425 716 1287
Size: 425 291 571
Fragments arranged by size:
571 425 291
BbeI G_GCGC'C
Cuts at: 0 79 237 484 1287
Size: 79 158 247 803
Fragments arranged by size:
803 247 158 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 514 1095 1287
Size: 514 581 192
Fragments arranged by size:
581 514 192
BbvCI CC'TCA_GC
Cuts at: 0 1274 1287
Size: 1274 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 153 187 378 412 1052 1086 1287
Size: 153 34 191 34 640 34 201
Fragments arranged by size:
640 201 191 153 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 478 1287
Size: 478 809
BglII A'GATC_T
Cuts at: 0 1016 1287
Size: 1016 271
Bme1580I G_kGCm'C
Cuts at: 0 813 1287
Size: 813 474
BmgBI CAC'GTC
Cuts at: 0 617 1287
Size: 617 670
BmrI ACTGGGnnnn_n'
Cuts at: 0 681 807 1287
Size: 681 126 480
Fragments arranged by size:
681 480 126
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1182 1287
Size: 1182 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1128 1202 1287
Size: 104 1024 74 85
Fragments arranged by size:
1024 104 85 74
Bpu10I CC'TnA_GC
Cuts at: 0 1274 1287
Size: 1274 13
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 890 1287
Size: 1 889 397
Fragments arranged by size:
889 397 1
BsaHI Gr'CG_yC
Cuts at: 0 76 234 481 1287
Size: 76 158 247 806
Fragments arranged by size:
806 247 158 76
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 102 132 1287
Size: 102 30 1155
Fragments arranged by size:
1155 102 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 417 1214 1287
Size: 417 797 73
Fragments arranged by size:
797 417 73
BseYI C'CCAG_C
Cuts at: 0 579 1287
Size: 579 708
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 372 1207 1287
Size: 24 348 835 80
Fragments arranged by size:
835 348 80 24
BsiEI CG_ry'CG
Cuts at: 0 326 833 1287
Size: 326 507 454
Fragments arranged by size:
507 454 326
BsiHKAI G_wGCw'C
Cuts at: 0 365 557 941 1287
Size: 365 192 384 346
Fragments arranged by size:
384 365 346 192
BsmI GAATG_Cn'
Cuts at: 0 976 1287
Size: 976 311
BsmBI CGTCTCn'nnnn_
Cuts at: 0 829 1287
Size: 829 458
Bsp1286I G_dGCh'C
Cuts at: 0 365 425 557 716 813 941 1287
Size: 365 60 132 159 97 128 346
Fragments arranged by size:
365 346 159 132 128 97 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1287
Size: 1046 241
BsrBI CCG'CTC
Cuts at: 0 462 891 1205 1287
Size: 462 429 314 82
Fragments arranged by size:
462 429 314 82
BsrFI r'CCGG_y
Cuts at: 0 230 573 1113 1287
Size: 230 343 540 174
Fragments arranged by size:
540 343 230 174
BssSI C'ACGA_G
Cuts at: 0 214 1287
Size: 214 1073
BstBI TT'CG_AA
Cuts at: 0 1062 1287
Size: 1062 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 450 1287
Size: 450 837
BstYI r'GATC_y
Cuts at: 0 1016 1023 1052 1287
Size: 1016 7 29 235
Fragments arranged by size:
1016 235 29 7
BtgI C'CryG_G
Cuts at: 0 152 449 791 1287
Size: 152 297 342 496
Fragments arranged by size:
496 342 297 152
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 423 1287
Size: 423 864
BtsI GCAGTG_nn'
Cuts at: 0 939 1195 1207 1232 1287
Size: 939 256 12 25 55
Fragments arranged by size:
939 256 55 25 12
EaeI y'GGCC_r
Cuts at: 0 203 452 470 548 749 839 1287
Size: 203 249 18 78 201 90 448
Fragments arranged by size:
448 249 203 201 90 78 18
EarI CTCTTCn'nnn_
Cuts at: 0 666 1287
Size: 666 621
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 78 1287
Size: 78 1209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 1128 1202 1287
Size: 104 1024 74 85
Fragments arranged by size:
1024 104 85 74
EcoO109I rG'GnC_Cy
Cuts at: 0 762 1287
Size: 762 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 1287
Size: 276 1011
HaeII r_GCGC'y
Cuts at: 0 79 237 289 449 469 484 724 1287
Size: 79 158 52 160 20 15 240 563
Fragments arranged by size:
563 240 160 158 79 52 20 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 906 938 1008 1040 1134 1166
Size: 127 32 747 32 70 32 94 32
Cuts at: 1166 1189 1221 1287
Size: 23 32 66
Fragments arranged by size:
747 127 94 70 66 32 32 32
32 32 23
KasI G'GCGC_C
Cuts at: 0 75 233 480 1287
Size: 75 158 247 807
Fragments arranged by size:
807 247 158 75
MluI A'CGCG_T
Cuts at: 0 511 1287
Size: 511 776
MscI TGG'CCA
Cuts at: 0 205 472 751 1287
Size: 205 267 279 536
Fragments arranged by size:
536 279 267 205
MslI CAynn'nnrTG
Cuts at: 0 67 334 491 865 1287
Size: 67 267 157 374 422
Fragments arranged by size:
422 374 267 157 67
MspA1I CmG'CkG
Cuts at: 0 776 1287
Size: 776 511
NaeI GCC'GGC
Cuts at: 0 1115 1287
Size: 1115 172
NarI GG'CG_CC
Cuts at: 0 76 234 481 1287
Size: 76 158 247 806
Fragments arranged by size:
806 247 158 76
NcoI C'CATG_G
Cuts at: 0 449 1287
Size: 449 838
NgoMIV G'CCGG_C
Cuts at: 0 1113 1287
Size: 1113 174
NspI r_CATG'y
Cuts at: 0 72 1287
Size: 72 1215
PflMI CCAn_nnn'nTGG
Cuts at: 0 127 1020 1287
Size: 127 893 267
Fragments arranged by size:
893 267 127
SapI GCTCTTCn'nnn_
Cuts at: 0 666 1287
Size: 666 621
SexAI A'CCwGG_T
Cuts at: 0 296 1287
Size: 296 991
SfoI GGC'GCC
Cuts at: 0 77 235 482 1287
Size: 77 158 247 805
Fragments arranged by size:
805 247 158 77
SmaI CCC'GGG
Cuts at: 0 13 612 1287
Size: 13 599 675
Fragments arranged by size:
675 599 13
StyI C'CwwG_G
Cuts at: 0 449 785 1027 1287
Size: 449 336 242 260
Fragments arranged by size:
449 336 260 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 847 864 1189 1287
Size: 847 17 325 98
Fragments arranged by size:
847 325 98 17
TatI w'GTAC_w
Cuts at: 0 593 1287
Size: 593 694
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 102 134 1287
Size: 102 32 1153
Fragments arranged by size:
1153 102 32
Tth111I GACn'n_nGTC
Cuts at: 0 298 1287
Size: 298 989
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 762 849 1287
Size: 762 87 438
Fragments arranged by size:
762 438 87
XmaI C'CCGG_G
Cuts at: 0 11 610 1287
Size: 11 599 677
Fragments arranged by size:
677 599 11
XmnI GAAnn'nnTTC
Cuts at: 0 259 1287
Size: 259 1028
Enzymes that do cut:
AflIII AlfI AlwNI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrFI BssSI BstBI BstXI BstYI BtgI BtgZI BtsI
EaeI EarI EciI Eco57MI EcoO109I FalI HaeII Hin4I
KasI MluI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PflMI SapI SexAI SfoI SmaI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI BlpI
BmtI BpuEI BsaAI BsaBI BsaWI BsiWI BspEI BspHI
BsrDI BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MmeI NdeI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI XbaI XhoI ZraI