Restriction map for
pFN21AE3514
(Linear) (Six-base) MAPSORT of: KIEE3514.seq Check: 3217 from: 1 to: 735
KIEE3514.orf
With 182 enzymes: SgfI *
March 22, 2012 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 351 735
Size: 351 384
AclI AA'CG_TT
Cuts at: 0 686 735
Size: 686 49
AfeI AGC'GCT
Cuts at: 0 421 735
Size: 421 314
BanI G'GyrC_C
Cuts at: 0 302 363 646 735
Size: 302 61 283 89
Fragments arranged by size:
302 283 89 61
BanII G_rGCy'C
Cuts at: 0 608 713 735
Size: 608 105 22
Fragments arranged by size:
608 105 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 632 735
Size: 632 103
BbvCI CC'TCA_GC
Cuts at: 0 695 735
Size: 695 40
BglI GCCn_nnn'nGGC
Cuts at: 0 431 735
Size: 431 304
BlpI GC'TnA_GC
Cuts at: 0 607 735
Size: 607 128
Bme1580I G_kGCm'C
Cuts at: 0 305 651 735
Size: 305 346 84
Fragments arranged by size:
346 305 84
BmtI G_CTAG'C
Cuts at: 0 145 735
Size: 145 590
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 640 661 735
Size: 522 118 21 74
Fragments arranged by size:
522 118 74 21
Bpu10I CC'TnA_GC
Cuts at: 0 695 735
Size: 695 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 735
Size: 64 671
BsaWI w'CCGG_w
Cuts at: 0 134 215 735
Size: 134 81 520
Fragments arranged by size:
520 134 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 735
Size: 673 62
BseYI C'CCAG_C
Cuts at: 0 413 735
Size: 413 322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 475 735
Size: 336 139 260
Fragments arranged by size:
336 260 139
BsiEI CG_ry'CG
Cuts at: 0 458 735
Size: 458 277
BsiHKAI G_wGCw'C
Cuts at: 0 61 389 468 608 735
Size: 61 328 79 140 127
Fragments arranged by size:
328 140 127 79 61
Bsp1286I G_dGCh'C
Cuts at: 0 61 305 389 468 608 651 713 735
Size: 61 244 84 79 140 43 62 22
Fragments arranged by size:
244 140 84 79 62 61 43 22
BspEI T'CCGG_A
Cuts at: 0 215 735
Size: 215 520
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 351 735
Size: 351 384
BsrBI CCG'CTC
Cuts at: 0 634 735
Size: 634 101
BsrDI GCAATG_nn'
Cuts at: 0 396 735
Size: 396 339
BsrFI r'CCGG_y
Cuts at: 0 389 735
Size: 389 346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 125 735
Size: 125 610
BtgI C'CryG_G
Cuts at: 0 69 735
Size: 69 666
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 277 735
Size: 277 458
EaeI y'GGCC_r
Cuts at: 0 228 455 735
Size: 228 227 280
Fragments arranged by size:
280 228 227
EagI C'GGCC_G
Cuts at: 0 455 735
Size: 455 280
EarI CTCTTCn'nnn_
Cuts at: 0 31 735
Size: 31 704
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 616 735
Size: 616 119
EcoICRI GAG'CTC
Cuts at: 0 606 735
Size: 606 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 640 661 735
Size: 522 118 21 74
Fragments arranged by size:
522 118 74 21
EcoO109I rG'GnC_Cy
Cuts at: 0 374 410 735
Size: 374 36 325
Fragments arranged by size:
374 325 36
HaeII r_GCGC'y
Cuts at: 0 423 522 735
Size: 423 99 213
Fragments arranged by size:
423 213 99
NcoI C'CATG_G
Cuts at: 0 69 735
Size: 69 666
NheI G'CTAG_C
Cuts at: 0 141 735
Size: 141 594
NspI r_CATG'y
Cuts at: 0 281 735
Size: 281 454
PasI CC'CwG_GG
Cuts at: 0 429 713 735
Size: 429 284 22
Fragments arranged by size:
429 284 22
PfoI T'CCnGG_A
Cuts at: 0 617 735
Size: 617 118
PpuMI rG'GwC_Cy
Cuts at: 0 374 410 735
Size: 374 36 325
Fragments arranged by size:
374 325 36
SacI G_AGCT'C
Cuts at: 0 608 735
Size: 608 127
SanDI GG'GwC_CC
Cuts at: 0 374 410 735
Size: 374 36 325
Fragments arranged by size:
374 325 36
SfcI C'TryA_G
Cuts at: 0 168 735
Size: 168 567
SmlI C'TyrA_G
Cuts at: 0 79 735
Size: 79 656
StyI C'CwwG_G
Cuts at: 0 69 735
Size: 69 666
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 735
Size: 229 32 474
Fragments arranged by size:
474 229 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 735
Size: 231 504
Enzymes that do cut:
AarI AclI AfeI BanI BanII BbsI BbvCI BglI
BlpI Bme1580I BmtI BpmI Bpu10I BpuEI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstAPI BtgI BtgZI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII NcoI NheI NspI
PasI PfoI PpuMI SacI SanDI SfcI SmlI StyI
TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI
BciVI BclI BfrBI BglII BmgBI BmrI BplI BsaI
BsaAI BsaBI BsaHI BsaXI BsiWI BsmI BsmBI BspHI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
XmnI ZraI