(Linear) (Six-base) MAPSORT of: KIEE3514.seq Check: 3217 from: 1 to: 735 KIEE3514.orf With 182 enzymes: SgfI * March 22, 2012 16:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 351 735 Size: 351 384 AclI AA'CG_TT Cuts at: 0 686 735 Size: 686 49 AfeI AGC'GCT Cuts at: 0 421 735 Size: 421 314 BanI G'GyrC_C Cuts at: 0 302 363 646 735 Size: 302 61 283 89 Fragments arranged by size: 302 283 89 61 BanII G_rGCy'C Cuts at: 0 608 713 735 Size: 608 105 22 Fragments arranged by size: 608 105 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 632 735 Size: 632 103 BbvCI CC'TCA_GC Cuts at: 0 695 735 Size: 695 40 BglI GCCn_nnn'nGGC Cuts at: 0 431 735 Size: 431 304 BlpI GC'TnA_GC Cuts at: 0 607 735 Size: 607 128 Bme1580I G_kGCm'C Cuts at: 0 305 651 735 Size: 305 346 84 Fragments arranged by size: 346 305 84 BmtI G_CTAG'C Cuts at: 0 145 735 Size: 145 590 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 522 640 661 735 Size: 522 118 21 74 Fragments arranged by size: 522 118 74 21 Bpu10I CC'TnA_GC Cuts at: 0 695 735 Size: 695 40 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 735 Size: 64 671 BsaWI w'CCGG_w Cuts at: 0 134 215 735 Size: 134 81 520 Fragments arranged by size: 520 134 81 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 673 735 Size: 673 62 BseYI C'CCAG_C Cuts at: 0 413 735 Size: 413 322 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 336 475 735 Size: 336 139 260 Fragments arranged by size: 336 260 139 BsiEI CG_ry'CG Cuts at: 0 458 735 Size: 458 277 BsiHKAI G_wGCw'C Cuts at: 0 61 389 468 608 735 Size: 61 328 79 140 127 Fragments arranged by size: 328 140 127 79 61 Bsp1286I G_dGCh'C Cuts at: 0 61 305 389 468 608 651 713 735 Size: 61 244 84 79 140 43 62 22 Fragments arranged by size: 244 140 84 79 62 61 43 22 BspEI T'CCGG_A Cuts at: 0 215 735 Size: 215 520 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 351 735 Size: 351 384 BsrBI CCG'CTC Cuts at: 0 634 735 Size: 634 101 BsrDI GCAATG_nn' Cuts at: 0 396 735 Size: 396 339 BsrFI r'CCGG_y Cuts at: 0 389 735 Size: 389 346 BstAPI GCAn_nnn'nTGC Cuts at: 0 125 735 Size: 125 610 BtgI C'CryG_G Cuts at: 0 69 735 Size: 69 666 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 277 735 Size: 277 458 EaeI y'GGCC_r Cuts at: 0 228 455 735 Size: 228 227 280 Fragments arranged by size: 280 228 227 EagI C'GGCC_G Cuts at: 0 455 735 Size: 455 280 EarI CTCTTCn'nnn_ Cuts at: 0 31 735 Size: 31 704 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 616 735 Size: 616 119 EcoICRI GAG'CTC Cuts at: 0 606 735 Size: 606 129 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 522 640 661 735 Size: 522 118 21 74 Fragments arranged by size: 522 118 74 21 EcoO109I rG'GnC_Cy Cuts at: 0 374 410 735 Size: 374 36 325 Fragments arranged by size: 374 325 36 HaeII r_GCGC'y Cuts at: 0 423 522 735 Size: 423 99 213 Fragments arranged by size: 423 213 99 NcoI C'CATG_G Cuts at: 0 69 735 Size: 69 666 NheI G'CTAG_C Cuts at: 0 141 735 Size: 141 594 NspI r_CATG'y Cuts at: 0 281 735 Size: 281 454 PasI CC'CwG_GG Cuts at: 0 429 713 735 Size: 429 284 22 Fragments arranged by size: 429 284 22 PfoI T'CCnGG_A Cuts at: 0 617 735 Size: 617 118 PpuMI rG'GwC_Cy Cuts at: 0 374 410 735 Size: 374 36 325 Fragments arranged by size: 374 325 36 SacI G_AGCT'C Cuts at: 0 608 735 Size: 608 127 SanDI GG'GwC_CC Cuts at: 0 374 410 735 Size: 374 36 325 Fragments arranged by size: 374 325 36 SfcI C'TryA_G Cuts at: 0 168 735 Size: 168 567 SmlI C'TyrA_G Cuts at: 0 79 735 Size: 79 656 StyI C'CwwG_G Cuts at: 0 69 735 Size: 69 666 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 229 261 735 Size: 229 32 474 Fragments arranged by size: 474 229 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 231 735 Size: 231 504 Enzymes that do cut: AarI AclI AfeI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmtI BpmI Bpu10I BpuEI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BtgI BtgZI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII NcoI NheI NspI PasI PfoI PpuMI SacI SanDI SfcI SmlI StyI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BplI BsaI BsaAI BsaBI BsaHI BsaXI BsiWI BsmI BsmBI BspHI BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI ZraI