Restriction map for
pFN21AE3511
(Linear) (Six-base) MAPSORT of: KIEE3511.seq Check: 9926 from: 1 to: 1191
KIEE3511.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AclI AA'CG_TT
Cuts at: 0 648 1191
Size: 648 543
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 461 863 1191
Size: 413 48 402 328
Fragments arranged by size:
413 402 328 48
AlwNI CAG_nnn'CTG
Cuts at: 0 187 609 658 1191
Size: 187 422 49 533
Fragments arranged by size:
533 422 187 49
BamHI G'GATC_C
Cuts at: 0 532 1191
Size: 532 659
BanII G_rGCy'C
Cuts at: 0 11 313 1191
Size: 11 302 878
Fragments arranged by size:
878 302 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 1052 1191
Size: 1052 139
BglI GCCn_nnn'nGGC
Cuts at: 0 103 1191
Size: 103 1088
BmrI ACTGGGnnnn_n'
Cuts at: 0 216 1191
Size: 216 975
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 49 1191
Size: 49 1142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1183 1191
Size: 1010 173 8
Fragments arranged by size:
1010 173 8
Bpu10I CC'TnA_GC
Cuts at: 0 79 1191
Size: 79 1112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 522 1191
Size: 522 669
BsaI GGTCTCn'nnnn_
Cuts at: 0 1159 1191
Size: 1159 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 509 539 1191
Size: 437 30 42 30 652
Fragments arranged by size:
652 437 42 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 493 1191
Size: 19 474 698
Fragments arranged by size:
698 474 19
BseYI C'CCAG_C
Cuts at: 0 357 990 1191
Size: 357 633 201
Fragments arranged by size:
633 357 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 1191
Size: 908 283
BsiEI CG_ry'CG
Cuts at: 0 139 1191
Size: 139 1052
BsiHKAI G_wGCw'C
Cuts at: 0 11 548 1191
Size: 11 537 643
Fragments arranged by size:
643 537 11
Bsp1286I G_dGCh'C
Cuts at: 0 11 313 548 1191
Size: 11 302 235 643
Fragments arranged by size:
643 302 235 11
BsrFI r'CCGG_y
Cuts at: 0 138 1191
Size: 138 1053
BstYI r'GATC_y
Cuts at: 0 495 532 1191
Size: 495 37 659
Fragments arranged by size:
659 495 37
Bsu36I CC'TnA_GG
Cuts at: 0 277 1191
Size: 277 914
BtgI C'CryG_G
Cuts at: 0 344 1191
Size: 344 847
BtsI GCAGTG_nn'
Cuts at: 0 300 607 1191
Size: 300 307 584
Fragments arranged by size:
584 307 300
EaeI y'GGCC_r
Cuts at: 0 136 1191
Size: 136 1055
EagI C'GGCC_G
Cuts at: 0 136 1191
Size: 136 1055
EarI CTCTTCn'nnn_
Cuts at: 0 208 1191
Size: 208 983
EcoICRI GAG'CTC
Cuts at: 0 9 1191
Size: 9 1182
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 461 863 1010 1183 1191
Size: 413 48 402 147 173 8
Fragments arranged by size:
413 402 173 147 48 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 23 1191
Size: 23 1168
EcoO109I rG'GnC_Cy
Cuts at: 0 184 1038 1191
Size: 184 854 153
Fragments arranged by size:
854 184 153
HaeII r_GCGC'y
Cuts at: 0 109 242 327 1191
Size: 109 133 85 864
Fragments arranged by size:
864 133 109 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 469 471 501 509 541 814 846
Size: 439 30 2 30 8 32 273 32
Cuts at: 846 1191
Size: 345
Fragments arranged by size:
439 345 273 32 32 30 30 8
2
MslI CAynn'nnrTG
Cuts at: 0 905 956 1191
Size: 905 51 235
Fragments arranged by size:
905 235 51
MspA1I CmG'CkG
Cuts at: 0 123 1191
Size: 123 1068
NcoI C'CATG_G
Cuts at: 0 344 1191
Size: 344 847
NspI r_CATG'y
Cuts at: 0 904 955 1191
Size: 904 51 236
Fragments arranged by size:
904 236 51
PflMI CCAn_nnn'nTGG
Cuts at: 0 187 306 1191
Size: 187 119 885
Fragments arranged by size:
885 187 119
PfoI T'CCnGG_A
Cuts at: 0 11 1040 1058 1160 1191
Size: 11 1029 18 102 31
Fragments arranged by size:
1029 102 31 18 11
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 584 616 1191
Size: 584 32 575
Fragments arranged by size:
584 575 32
PpuMI rG'GwC_Cy
Cuts at: 0 1038 1191
Size: 1038 153
SacI G_AGCT'C
Cuts at: 0 11 1191
Size: 11 1180
SfcI C'TryA_G
Cuts at: 0 659 668 1191
Size: 659 9 523
Fragments arranged by size:
659 523 9
SmlI C'TyrA_G
Cuts at: 0 537 1191
Size: 537 654
SphI G_CATG'C
Cuts at: 0 955 1191
Size: 955 236
StuI AGG'CCT
Cuts at: 0 728 1191
Size: 728 463
StyI C'CwwG_G
Cuts at: 0 153 344 513 1191
Size: 153 191 169 678
Fragments arranged by size:
678 191 169 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 1191
Size: 247 32 912
Fragments arranged by size:
912 247 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 188 672 1191
Size: 188 484 519
Fragments arranged by size:
519 484 188
XmnI GAAnn'nnTTC
Cuts at: 0 599 1191
Size: 599 592
Enzymes that do cut:
AclI AcuI AlwNI BamHI BanII BbsI BglI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrFI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I MslI MspA1I NcoI NspI PflMI PfoI
PpiI PpuMI SacI SfcI SmlI SphI StuI StyI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmtI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XhoI XmaI
ZraI