Restriction map for
pFN21AE3510
(Linear) (Six-base) MAPSORT of: KIEE3510.seq Check: 7263 from: 1 to: 1209
KIEE3510.orf
With 182 enzymes: SgfI *
March 22, 2012 16:35 ..
AatII G_ACGT'C
Cuts at: 0 308 1209
Size: 308 901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 678 1073 1209
Size: 678 395 136
Fragments arranged by size:
678 395 136
AleI CACnn'nnGTG
Cuts at: 0 136 1209
Size: 136 1073
AlwNI CAG_nnn'CTG
Cuts at: 0 117 1209
Size: 117 1092
ApaI G_GGCC'C
Cuts at: 0 1182 1209
Size: 1182 27
AvaI C'yCGr_G
Cuts at: 0 923 1209
Size: 923 286
BamHI G'GATC_C
Cuts at: 0 534 1209
Size: 534 675
BanII G_rGCy'C
Cuts at: 0 179 365 608 1182 1209
Size: 179 186 243 574 27
Fragments arranged by size:
574 243 186 179 27
BbvCI CC'TCA_GC
Cuts at: 0 413 629 652 1209
Size: 413 216 23 557
Fragments arranged by size:
557 413 216 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1015 1049 1209
Size: 1015 34 160
Fragments arranged by size:
1015 160 34
BclI T'GATC_A
Cuts at: 0 872 1209
Size: 872 337
Bme1580I G_kGCm'C
Cuts at: 0 1170 1182 1209
Size: 1170 12 27
Fragments arranged by size:
1170 27 12
BmgBI CAC'GTC
Cuts at: 0 702 1209
Size: 702 507
BmrI ACTGGGnnnn_n'
Cuts at: 0 54 1209
Size: 54 1155
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 379 1209
Size: 379 830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1209
Size: 349 860
Bpu10I CC'TnA_GC
Cuts at: 0 413 629 652 1209
Size: 413 216 23 557
Fragments arranged by size:
557 413 216 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 881 1209
Size: 881 328
BsaBI GATnn'nnATC
Cuts at: 0 382 580 1209
Size: 382 198 629
Fragments arranged by size:
629 382 198
BsaHI Gr'CG_yC
Cuts at: 0 305 1209
Size: 305 904
BsaWI w'CCGG_w
Cuts at: 0 8 1209
Size: 8 1201
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 382 1209
Size: 208 174 827
Fragments arranged by size:
827 208 174
BseYI C'CCAG_C
Cuts at: 0 998 1209
Size: 998 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1130 1209
Size: 403 727 79
Fragments arranged by size:
727 403 79
BsiHKAI G_wGCw'C
Cuts at: 0 608 1209
Size: 608 601
Bsp1286I G_dGCh'C
Cuts at: 0 179 365 608 1170 1182 1209
Size: 179 186 243 562 12 27
Fragments arranged by size:
562 243 186 179 27 12
BspEI T'CCGG_A
Cuts at: 0 8 1209
Size: 8 1201
BsrBI CCG'CTC
Cuts at: 0 1141 1209
Size: 1141 68
BsrDI GCAATG_nn'
Cuts at: 0 316 1209
Size: 316 893
BstAPI GCAn_nnn'nTGC
Cuts at: 0 705 1209
Size: 705 504
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 1209
Size: 138 1071
BstYI r'GATC_y
Cuts at: 0 534 842 1209
Size: 534 308 367
Fragments arranged by size:
534 367 308
Bsu36I CC'TnA_GG
Cuts at: 0 520 848 1209
Size: 520 328 361
Fragments arranged by size:
520 361 328
BtsI GCAGTG_nn'
Cuts at: 0 1118 1209
Size: 1118 91
DraIII CAC_nnn'GTG
Cuts at: 0 705 1209
Size: 705 504
DrdI GACnn_nn'nnGTC
Cuts at: 0 448 1209
Size: 448 761
EaeI y'GGCC_r
Cuts at: 0 39 1209
Size: 39 1170
EcoICRI GAG'CTC
Cuts at: 0 606 1209
Size: 606 603
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 678 1073 1209
Size: 349 329 395 136
Fragments arranged by size:
395 349 329 136
EcoNI CCTnn'n_nnAGG
Cuts at: 0 251 1209
Size: 251 958
EcoO109I rG'GnC_Cy
Cuts at: 0 1179 1209
Size: 1179 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 87 1209
Size: 87 1122
FspI TGC'GCA
Cuts at: 0 17 1209
Size: 17 1192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 976 1008 1209
Size: 802 32 142 32 201
Fragments arranged by size:
802 201 142 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1209
Size: 562 647
MslI CAynn'nnrTG
Cuts at: 0 136 1209
Size: 136 1073
MspA1I CmG'CkG
Cuts at: 0 417 656 934 1029 1194 1200 1209
Size: 417 239 278 95 165 6 9
Fragments arranged by size:
417 278 239 165 95 9 6
NruI TCG'CGA
Cuts at: 0 782 1209
Size: 782 427
NspI r_CATG'y
Cuts at: 0 342 682 1209
Size: 342 340 527
Fragments arranged by size:
527 342 340
PfoI T'CCnGG_A
Cuts at: 0 365 794 1209
Size: 365 429 415
Fragments arranged by size:
429 415 365
PspOMI G'GGCC_C
Cuts at: 0 1178 1209
Size: 1178 31
PstI C_TGCA'G
Cuts at: 0 1199 1209
Size: 1199 10
PvuII CAG'CTG
Cuts at: 0 417 656 1194 1200 1209
Size: 417 239 538 6 9
Fragments arranged by size:
538 417 239 9 6
SacI G_AGCT'C
Cuts at: 0 608 1209
Size: 608 601
SexAI A'CCwGG_T
Cuts at: 0 590 667 1209
Size: 590 77 542
Fragments arranged by size:
590 542 77
SfcI C'TryA_G
Cuts at: 0 1195 1209
Size: 1195 14
SphI G_CATG'C
Cuts at: 0 342 1209
Size: 342 867
StuI AGG'CCT
Cuts at: 0 1008 1209
Size: 1008 201
StyI C'CwwG_G
Cuts at: 0 1084 1209
Size: 1084 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 752 810 1209
Size: 752 58 399
Fragments arranged by size:
752 399 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 820 852 1209
Size: 820 32 357
Fragments arranged by size:
820 357 32
ZraI GAC'GTC
Cuts at: 0 306 1209
Size: 306 903
Enzymes that do cut:
AatII AcuI AleI AlwNI ApaI AvaI BamHI BanII
BbvCI BcgI BclI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BsrBI BsrDI BstAPI BstXI BstYI
Bsu36I BtsI DraIII DrdI EaeI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI Hin4I MmeI MslI MspA1I NruI
NspI PfoI PspOMI PstI PvuII SacI SexAI SfcI
SphI StuI StyI TaqII TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbsI BciVI BfrBI BglI
BglII BlpI BmtI BpuEI BsaAI BsaXI BsiEI BsiWI
BsmI BsmBI BspHI BspMI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI
EagI EarI EciI EcoRI EcoRV FseI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI XmnI