Restriction map for
pFN21AE3507
(Linear) (Six-base) MAPSORT of: KIEE3507.seq Check: 2070 from: 1 to: 2787
KIEE3507.orf
With 182 enzymes: SgfI *
July 3, 2012 18:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1710 1785 2787
Size: 1710 75 1002
Fragments arranged by size:
1710 1002 75
AatII G_ACGT'C
Cuts at: 0 1705 2787
Size: 1705 1082
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1129 1338 2787
Size: 1129 209 1449
Fragments arranged by size:
1449 1129 209
AflIII A'CryG_T
Cuts at: 0 311 2787
Size: 311 2476
AgeI A'CCGG_T
Cuts at: 0 1322 2787
Size: 1322 1465
AhdI GACnn_n'nnGTC
Cuts at: 0 45 1751 2787
Size: 45 1706 1036
Fragments arranged by size:
1706 1036 45
AleI CACnn'nnGTG
Cuts at: 0 1864 2029 2787
Size: 1864 165 758
Fragments arranged by size:
1864 758 165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1861 2372 2497 2787
Size: 1861 511 125 290
Fragments arranged by size:
1861 511 290 125
AlwNI CAG_nnn'CTG
Cuts at: 0 192 1294 1560 1601 2133 2427 2787
Size: 192 1102 266 41 532 294 360
Fragments arranged by size:
1102 532 360 294 266 192 41
ApaI G_GGCC'C
Cuts at: 0 222 1001 2787
Size: 222 779 1786
Fragments arranged by size:
1786 779 222
ApaLI G'TGCA_C
Cuts at: 0 412 1030 2787
Size: 412 618 1757
Fragments arranged by size:
1757 618 412
ApoI r'AATT_y
Cuts at: 0 447 1420 2787
Size: 447 973 1367
Fragments arranged by size:
1367 973 447
AvaI C'yCGr_G
Cuts at: 0 22 91 135 1282 1400 1678 2787
Size: 22 69 44 1147 118 278 1109
Fragments arranged by size:
1147 1109 278 118 69 44 22
BamHI G'GATC_C
Cuts at: 0 734 1043 1079 2787
Size: 734 309 36 1708
Fragments arranged by size:
1708 734 309 36
BanI G'GyrC_C
Cuts at: 0 105 515 1312 1609 1920 2787
Size: 105 410 797 297 311 867
Fragments arranged by size:
867 797 410 311 297 105
BanII G_rGCy'C
Cuts at: 0 222 1001 1277 1655 1811 2060 2096 2199
Size: 222 779 276 378 156 249 36 103
Cuts at: 2199 2787
Size: 588
Fragments arranged by size:
779 588 378 276 249 222 156 103
36
BbeI G_GCGC'C
Cuts at: 0 109 1316 2787
Size: 109 1207 1471
Fragments arranged by size:
1471 1207 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 969 1332 2665 2787
Size: 969 363 1333 122
Fragments arranged by size:
1333 969 363 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 371 405 1821 1855 2787
Size: 371 34 1416 34 932
Fragments arranged by size:
1416 932 371 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 545 1659 2787
Size: 545 1114 1128
Fragments arranged by size:
1128 1114 545
BlpI GC'TnA_GC
Cuts at: 0 1271 2787
Size: 1271 1516
Bme1580I G_kGCm'C
Cuts at: 0 158 222 416 1001 1034 1612 2026 2070
Size: 158 64 194 585 33 578 414 44
Cuts at: 2070 2787
Size: 717
Fragments arranged by size:
717 585 578 414 194 158 64 44
33
BmgBI CAC'GTC
Cuts at: 0 1377 2787
Size: 1377 1410
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1185 2787
Size: 531 654 1602
Fragments arranged by size:
1602 654 531
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 708 2787
Size: 708 2079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 285 457 468 861 1033 1405 2068
Size: 169 116 172 11 393 172 372 663
Cuts at: 2068 2126 2404 2752 2787
Size: 58 278 348 35
Fragments arranged by size:
663 393 372 348 278 172 172 169
116 58 35 11
Bpu10I CC'TnA_GC
Cuts at: 0 985 2258 2705 2787
Size: 985 1273 447 82
Fragments arranged by size:
1273 985 447 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1984 2354 2787
Size: 1984 370 433
Fragments arranged by size:
1984 433 370
BsaI GGTCTCn'nnnn_
Cuts at: 0 298 837 2572 2787
Size: 298 539 1735 215
Fragments arranged by size:
1735 539 298 215
BsaAI yAC'GTr
Cuts at: 0 1224 2787
Size: 1224 1563
BsaBI GATnn'nnATC
Cuts at: 0 2245 2787
Size: 2245 542
BsaHI Gr'CG_yC
Cuts at: 0 83 106 537 1313 1370 1702 2159 2787
Size: 83 23 431 776 57 332 457 628
Fragments arranged by size:
776 628 457 431 332 83 57 23
BsaWI w'CCGG_w
Cuts at: 0 248 1322 2787
Size: 248 1074 1465
Fragments arranged by size:
1465 1074 248
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 844 1243 2787
Size: 697 147 399 1544
Fragments arranged by size:
1544 697 399 147
BseYI C'CCAG_C
Cuts at: 0 696 1256 1655 2019 2265 2437 2787
Size: 696 560 399 364 246 172 350
Fragments arranged by size:
696 560 399 364 350 246 172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 1813 1885 1888 1963 2345 2413 2467
Size: 246 1567 72 3 75 382 68 54
Cuts at: 2467 2787
Size: 320
Fragments arranged by size:
1567 382 320 246 75 72 68 54
3
BsiEI CG_ry'CG
Cuts at: 0 1323 2193 2787
Size: 1323 870 594
Fragments arranged by size:
1323 870 594
BsiHKAI G_wGCw'C
Cuts at: 0 293 416 694 1034 1640 1769 2060 2207
Size: 293 123 278 340 606 129 291 147
Cuts at: 2207 2787
Size: 580
Fragments arranged by size:
606 580 340 293 291 278 147 129
123
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2453 2770 2787
Size: 2453 317 17
Fragments arranged by size:
2453 317 17
Bsp1286I G_dGCh'C
Cuts at: 0 158 222 293 416 694 1001 1034 1277
Size: 158 64 71 123 278 307 33 243
Cuts at: 1277 1612 1640 1655 1769 1811 2026 2060 2070
Size: 335 28 15 114 42 215 34 10
Cuts at: 2070 2096 2199 2207 2787
Size: 26 103 8 580
Fragments arranged by size:
580 335 307 278 243 215 158 123
114 103 71 64 42 34 33 28
26 15 10 8
BspEI T'CCGG_A
Cuts at: 0 248 2787
Size: 248 2539
BspHI T'CATG_A
Cuts at: 0 878 2654 2787
Size: 878 1776 133
Fragments arranged by size:
1776 878 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1046 1710 1785 2534 2787
Size: 1046 664 75 749 253
Fragments arranged by size:
1046 749 664 253 75
BsrBI CCG'CTC
Cuts at: 0 240 876 1740 2787
Size: 240 636 864 1047
Fragments arranged by size:
1047 864 636 240
BsrFI r'CCGG_y
Cuts at: 0 85 1322 1917 2738 2787
Size: 85 1237 595 821 49
Fragments arranged by size:
1237 821 595 85 49
BssSI C'ACGA_G
Cuts at: 0 624 1393 2787
Size: 624 769 1394
Fragments arranged by size:
1394 769 624
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1038 1920 2621 2787
Size: 1038 882 701 166
Fragments arranged by size:
1038 882 701 166
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1500 2787
Size: 1500 1287
BstYI r'GATC_y
Cuts at: 0 245 734 1043 1079 1211 1550 2549 2787
Size: 245 489 309 36 132 339 999 238
Fragments arranged by size:
999 489 339 309 245 238 132 36
Bsu36I CC'TnA_GG
Cuts at: 0 827 1295 2787
Size: 827 468 1492
Fragments arranged by size:
1492 827 468
BtgI C'CryG_G
Cuts at: 0 7 109 713 1862 2787
Size: 7 102 604 1149 925
Fragments arranged by size:
1149 925 604 102 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1824 2787
Size: 1824 963
BtsI GCAGTG_nn'
Cuts at: 0 1756 2165 2344 2787
Size: 1756 409 179 443
Fragments arranged by size:
1756 443 409 179
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1161 1196 2787
Size: 1161 35 1591
Fragments arranged by size:
1591 1161 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 312 1375 2787
Size: 312 1063 1412
Fragments arranged by size:
1412 1063 312
EaeI y'GGCC_r
Cuts at: 0 611 1502 1709 2720 2787
Size: 611 891 207 1011 67
Fragments arranged by size:
1011 891 611 207 67
EarI CTCTTCn'nnn_
Cuts at: 0 154 781 2372 2787
Size: 154 627 1591 415
Fragments arranged by size:
1591 627 415 154
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 45 194 1742 1899 2787
Size: 45 149 1548 157 888
Fragments arranged by size:
1548 888 157 149 45
EcoICRI GAG'CTC
Cuts at: 0 2058 2787
Size: 2058 729
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 285 457 468 861 1033 1129 1338
Size: 169 116 172 11 393 172 96 209
Cuts at: 1338 1405 2068 2126 2404 2752 2787
Size: 67 663 58 278 348 35
Fragments arranged by size:
663 393 348 278 209 172 172 169
116 96 67 58 35 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 801 988 2787
Size: 801 187 1799
Fragments arranged by size:
1799 801 187
EcoO109I rG'GnC_Cy
Cuts at: 0 218 219 752 823 997 1260 1291 1497
Size: 218 1 533 71 174 263 31 206
Cuts at: 1497 1557 1974 2645 2787
Size: 60 417 671 142
Fragments arranged by size:
671 533 417 263 218 206 174 142
71 60 31 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 147 483 606 2166 2787
Size: 147 336 123 1560 621
Fragments arranged by size:
1560 621 336 147 123
FspI TGC'GCA
Cuts at: 0 393 1849 1936 2787
Size: 393 1456 87 851
Fragments arranged by size:
1456 851 393 87
HaeII r_GCGC'y
Cuts at: 0 109 132 1012 1316 1892 2787
Size: 109 23 880 304 576 895
Fragments arranged by size:
895 880 576 304 109 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 836 868 1189 1221 1737 1769
Size: 31 32 773 32 321 32 516 32
Cuts at: 1769 2787
Size: 1018
Fragments arranged by size:
1018 773 516 321 32 32 32 32
31
HindIII A'AGCT_T
Cuts at: 0 1816 2787
Size: 1816 971
KasI G'GCGC_C
Cuts at: 0 105 1312 2787
Size: 105 1207 1475
Fragments arranged by size:
1475 1207 105
MscI TGG'CCA
Cuts at: 0 613 1504 2787
Size: 613 891 1283
Fragments arranged by size:
1283 891 613
MslI CAynn'nnrTG
Cuts at: 0 1516 1789 1864 1942 1967 2029 2787
Size: 1516 273 75 78 25 62 758
Fragments arranged by size:
1516 758 273 78 75 62 25
MspA1I CmG'CkG
Cuts at: 0 9 68 111 435 455 620 839 1248
Size: 9 59 43 324 20 165 219 409
Cuts at: 1248 1256 1659 2019 2599 2691 2763 2787
Size: 8 403 360 580 92 72 24
Fragments arranged by size:
580 409 403 360 324 219 165 92
72 59 43 24 20 9 8
NarI GG'CG_CC
Cuts at: 0 106 1313 2787
Size: 106 1207 1474
Fragments arranged by size:
1474 1207 106
NcoI C'CATG_G
Cuts at: 0 1862 2787
Size: 1862 925
NdeI CA'TA_TG
Cuts at: 0 282 2787
Size: 282 2505
NruI TCG'CGA
Cuts at: 0 1757 2787
Size: 1757 1030
NspI r_CATG'y
Cuts at: 0 1962 2126 2787
Size: 1962 164 661
Fragments arranged by size:
1962 661 164
PasI CC'CwG_GG
Cuts at: 0 214 1140 1619 1630 2604 2787
Size: 214 926 479 11 974 183
Fragments arranged by size:
974 926 479 214 183 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 1619 2787
Size: 192 1427 1168
Fragments arranged by size:
1427 1168 192
PfoI T'CCnGG_A
Cuts at: 0 484 1553 2153 2249 2420 2787
Size: 484 1069 600 96 171 367
Fragments arranged by size:
1069 600 484 367 171 96
PmlI CAC'GTG
Cuts at: 0 1224 2787
Size: 1224 1563
PpuMI rG'GwC_Cy
Cuts at: 0 1260 1497 1557 2787
Size: 1260 237 60 1230
Fragments arranged by size:
1260 1230 237 60
PshAI GACnn'nnGTC
Cuts at: 0 86 2787
Size: 86 2701
PspOMI G'GGCC_C
Cuts at: 0 218 997 2787
Size: 218 779 1790
Fragments arranged by size:
1790 779 218
PspXI vC'TCGA_Gb
Cuts at: 0 1678 2787
Size: 1678 1109
PstI C_TGCA'G
Cuts at: 0 368 674 1721 2787
Size: 368 306 1047 1066
Fragments arranged by size:
1066 1047 368 306
PvuII CAG'CTG
Cuts at: 0 435 620 1248 2787
Size: 435 185 628 1539
Fragments arranged by size:
1539 628 435 185
SacI G_AGCT'C
Cuts at: 0 2060 2787
Size: 2060 727
SacII CC_GC'GG
Cuts at: 0 10 112 2787
Size: 10 102 2675
Fragments arranged by size:
2675 102 10
SapI GCTCTTCn'nnn_
Cuts at: 0 154 2372 2787
Size: 154 2218 415
Fragments arranged by size:
2218 415 154
ScaI AGT'ACT
Cuts at: 0 346 2787
Size: 346 2441
SfcI C'TryA_G
Cuts at: 0 364 670 1717 2181 2787
Size: 364 306 1047 464 606
Fragments arranged by size:
1047 606 464 364 306
SfoI GGC'GCC
Cuts at: 0 107 1314 2787
Size: 107 1207 1473
Fragments arranged by size:
1473 1207 107
SmaI CCC'GGG
Cuts at: 0 137 2787
Size: 137 2650
SmlI C'TyrA_G
Cuts at: 0 1400 1678 1999 2369 2787
Size: 1400 278 321 370 418
Fragments arranged by size:
1400 418 370 321 278
SphI G_CATG'C
Cuts at: 0 1962 2787
Size: 1962 825
StuI AGG'CCT
Cuts at: 0 2008 2787
Size: 2008 779
StyI C'CwwG_G
Cuts at: 0 1001 1493 1778 1862 2787
Size: 1001 492 285 84 925
Fragments arranged by size:
1001 925 492 285 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1316 2207 2346 2787
Size: 1316 891 139 441
Fragments arranged by size:
1316 891 441 139
TatI w'GTAC_w
Cuts at: 0 253 344 2787
Size: 253 91 2443
Fragments arranged by size:
2443 253 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 535 567 2787
Size: 535 32 2220
Fragments arranged by size:
2220 535 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1008 1137 1704 2787
Size: 1008 129 567 1083
Fragments arranged by size:
1083 1008 567 129
XhoI C'TCGA_G
Cuts at: 0 1400 1678 2787
Size: 1400 278 1109
Fragments arranged by size:
1400 1109 278
XmaI C'CCGG_G
Cuts at: 0 135 2787
Size: 135 2652
XmnI GAAnn'nnTTC
Cuts at: 0 571 2407 2787
Size: 571 1836 380
Fragments arranged by size:
1836 571 380
ZraI GAC'GTC
Cuts at: 0 1703 2787
Size: 1703 1084
Enzymes that do cut:
AarI AatII AcuI AflIII AgeI AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BcgI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HindIII KasI MscI MslI MspA1I NarI
NcoI NdeI NruI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SacII
SapI ScaI SfcI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AloI AscI AseI
AsiSI AvrII BaeI BbvCI BciVI BclI BfrBI BglII
BmtI BsaXI BsiWI BsmI BsrDI BsrGI BssHII BstBI
BstEII BstZ17I ClaI DraI DraIII EagI EcoRI EcoRV
FseI FspAI HincII HpaI KpnI MfeI MluI MmeI
NaeI NgoMIV NheI NotI NsiI PacI PciI PmeI
PpiI PsiI PsrI PvuI RsrII SalI SanDI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI