Restriction map for
pFN21AE3501
(Linear) (Six-base) MAPSORT of: KIEE3501.seq Check: 9651 from: 1 to: 3666
KIEE3501.orf
With 182 enzymes: SgfI *
March 22, 2012 16:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2283 3666
Size: 2283 1383
Acc65I G'GTAC_C
Cuts at: 0 1578 3666
Size: 1578 2088
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2383 2585 2638 2885 3128 3442 3666
Size: 2383 202 53 247 243 314 224
Fragments arranged by size:
2383 314 247 243 224 202 53
AflII C'TTAA_G
Cuts at: 0 711 2371 3666
Size: 711 1660 1295
Fragments arranged by size:
1660 1295 711
AflIII A'CryG_T
Cuts at: 0 2747 3666
Size: 2747 919
AleI CACnn'nnGTG
Cuts at: 0 1633 3666
Size: 1633 2033
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1008 1651 1979 3666
Size: 1008 643 328 1687
Fragments arranged by size:
1687 1008 643 328
AlwNI CAG_nnn'CTG
Cuts at: 0 246 747 1366 3666
Size: 246 501 619 2300
Fragments arranged by size:
2300 619 501 246
ApaLI G'TGCA_C
Cuts at: 0 993 3666
Size: 993 2673
ApoI r'AATT_y
Cuts at: 0 1113 1909 2419 2546 3504 3588 3666
Size: 1113 796 510 127 958 84 78
Fragments arranged by size:
1113 958 796 510 127 84 78
AseI AT'TA_AT
Cuts at: 0 2704 3666
Size: 2704 962
AvaI C'yCGr_G
Cuts at: 0 310 674 3666
Size: 310 364 2992
Fragments arranged by size:
2992 364 310
BamHI G'GATC_C
Cuts at: 0 150 3666
Size: 150 3516
BanI G'GyrC_C
Cuts at: 0 324 1417 1578 1619 3365 3666
Size: 324 1093 161 41 1746 301
Fragments arranged by size:
1746 1093 324 301 161 41
BanII G_rGCy'C
Cuts at: 0 681 698 977 3666
Size: 681 17 279 2689
Fragments arranged by size:
2689 681 279 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 2369 3448 3666
Size: 2369 1079 218
Fragments arranged by size:
2369 1079 218
BbvCI CC'TCA_GC
Cuts at: 0 1504 3666
Size: 1504 2162
BciVI GTATCCnnnnn_n'
Cuts at: 0 1288 3666
Size: 1288 2378
Bme1580I G_kGCm'C
Cuts at: 0 327 997 1422 3666
Size: 327 670 425 2244
Fragments arranged by size:
2244 670 425 327
BmrI ACTGGGnnnn_n'
Cuts at: 0 496 1007 2237 3556 3666
Size: 496 511 1230 1319 110
Fragments arranged by size:
1319 1230 511 496 110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1349 1427 1481 3278 3666
Size: 604 745 78 54 1797 388
Fragments arranged by size:
1797 745 604 388 78 54
Bpu10I CC'TnA_GC
Cuts at: 0 1504 3666
Size: 1504 2162
BsaI GGTCTCn'nnnn_
Cuts at: 0 113 3666
Size: 113 3553
BsaBI GATnn'nnATC
Cuts at: 0 1304 2332 3666
Size: 1304 1028 1334
Fragments arranged by size:
1334 1304 1028
BsaHI Gr'CG_yC
Cuts at: 0 82 3666
Size: 82 3584
BsaWI w'CCGG_w
Cuts at: 0 122 187 553 1048 3666
Size: 122 65 366 495 2618
Fragments arranged by size:
2618 495 366 122 65
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2223 2253 2277 2307 2702 2732 3666
Size: 2223 30 24 30 395 30 934
Fragments arranged by size:
2223 934 395 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 35 271 1664 2128 3613 3666
Size: 35 236 1393 464 1485 53
Fragments arranged by size:
1485 1393 464 236 53 35
BseYI C'CCAG_C
Cuts at: 0 246 3311 3666
Size: 246 3065 355
Fragments arranged by size:
3065 355 246
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2255 3666
Size: 2255 1411
BsiHKAI G_wGCw'C
Cuts at: 0 698 997 2013 3666
Size: 698 299 1016 1653
Fragments arranged by size:
1653 1016 698 299
BsmI GAATG_Cn'
Cuts at: 0 3087 3666
Size: 3087 579
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3269 3666
Size: 3269 397
Bsp1286I G_dGCh'C
Cuts at: 0 327 681 698 977 997 1422 2013 3666
Size: 327 354 17 279 20 425 591 1653
Fragments arranged by size:
1653 591 425 354 327 279 20 17
BspEI T'CCGG_A
Cuts at: 0 553 3666
Size: 553 3113
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 2283 3666
Size: 299 1984 1383
Fragments arranged by size:
1984 1383 299
BsrBI CCG'CTC
Cuts at: 0 551 3666
Size: 551 3115
BsrDI GCAATG_nn'
Cuts at: 0 1883 3666
Size: 1883 1783
BssSI C'ACGA_G
Cuts at: 0 229 3666
Size: 229 3437
BstAPI GCAn_nnn'nTGC
Cuts at: 0 243 3559 3666
Size: 243 3316 107
Fragments arranged by size:
3316 243 107
BstBI TT'CG_AA
Cuts at: 0 3246 3666
Size: 3246 420
BstEII G'GTnAC_C
Cuts at: 0 261 490 2364 3109 3666
Size: 261 229 1874 745 557
Fragments arranged by size:
1874 745 557 261 229
BstXI CCAn_nnnn'nTGG
Cuts at: 0 877 2026 2799 3666
Size: 877 1149 773 867
Fragments arranged by size:
1149 877 867 773
BstYI r'GATC_y
Cuts at: 0 150 2327 3666
Size: 150 2177 1339
Fragments arranged by size:
2177 1339 150
Bsu36I CC'TnA_GG
Cuts at: 0 53 839 859 1142 1349 3666
Size: 53 786 20 283 207 2317
Fragments arranged by size:
2317 786 283 207 53 20
BtgI C'CryG_G
Cuts at: 0 1623 2360 2792 3593 3666
Size: 1623 737 432 801 73
Fragments arranged by size:
1623 801 737 432 73
BtsI GCAGTG_nn'
Cuts at: 0 375 651 3666
Size: 375 276 3015
Fragments arranged by size:
3015 375 276
DraIII CAC_nnn'GTG
Cuts at: 0 2604 3666
Size: 2604 1062
DrdI GACnn_nn'nnGTC
Cuts at: 0 72 3666
Size: 72 3594
EaeI y'GGCC_r
Cuts at: 0 219 3666
Size: 219 3447
EarI CTCTTCn'nnn_
Cuts at: 0 703 1169 1794 3602 3666
Size: 703 466 625 1808 64
Fragments arranged by size:
1808 703 625 466 64
EcoICRI GAG'CTC
Cuts at: 0 696 3666
Size: 696 2970
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1349 1427 1481 2383 2585 2638 2885
Size: 604 745 78 54 902 202 53 247
Cuts at: 2885 3128 3278 3442 3666
Size: 243 150 164 224
Fragments arranged by size:
902 745 604 247 243 224 202 164
150 78 54 53
EcoO109I rG'GnC_Cy
Cuts at: 0 1146 3666
Size: 1146 2520
EcoRI G'AATT_C
Cuts at: 0 2419 3666
Size: 2419 1247
EcoRV GAT'ATC
Cuts at: 0 757 3666
Size: 757 2909
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2223 2248 2255 2280 2707 2739 3603 3635
Size: 2223 25 7 25 427 32 864 32
Cuts at: 3635 3666
Size: 31
Fragments arranged by size:
2223 864 427 32 32 31 25 25
7
HincII GTy'rAC
Cuts at: 0 525 3666
Size: 525 3141
HindIII A'AGCT_T
Cuts at: 0 439 1769 3666
Size: 439 1330 1897
Fragments arranged by size:
1897 1330 439
HpaI GTT'AAC
Cuts at: 0 525 3666
Size: 525 3141
KpnI G_GTAC'C
Cuts at: 0 1582 3666
Size: 1582 2084
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 2007 3666
Size: 392 1615 1659
Fragments arranged by size:
1659 1615 392
MslI CAynn'nnrTG
Cuts at: 0 628 1633 2797 3666
Size: 628 1005 1164 869
Fragments arranged by size:
1164 1005 869 628
MspA1I CmG'CkG
Cuts at: 0 225 243 1366 1503 3666
Size: 225 18 1123 137 2163
Fragments arranged by size:
2163 1123 225 137 18
NcoI C'CATG_G
Cuts at: 0 1623 2360 2792 3666
Size: 1623 737 432 874
Fragments arranged by size:
1623 874 737 432
NdeI CA'TA_TG
Cuts at: 0 1554 3666
Size: 1554 2112
NspI r_CATG'y
Cuts at: 0 523 637 667 709 2751 3666
Size: 523 114 30 42 2042 915
Fragments arranged by size:
2042 915 523 114 42 30
PciI A'CATG_T
Cuts at: 0 2747 3666
Size: 2747 919
PflMI CCAn_nnn'nTGG
Cuts at: 0 1588 3666
Size: 1588 2078
PfoI T'CCnGG_A
Cuts at: 0 136 446 1458 3255 3666
Size: 136 310 1012 1797 411
Fragments arranged by size:
1797 1012 411 310 136
PpuMI rG'GwC_Cy
Cuts at: 0 1146 3666
Size: 1146 2520
PshAI GACnn'nnGTC
Cuts at: 0 2355 3666
Size: 2355 1311
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2939 2971 3666
Size: 2939 32 695
Fragments arranged by size:
2939 695 32
PstI C_TGCA'G
Cuts at: 0 987 2586 2898 3666
Size: 987 1599 312 768
Fragments arranged by size:
1599 987 768 312
PvuII CAG'CTG
Cuts at: 0 243 1366 1503 3666
Size: 243 1123 137 2163
Fragments arranged by size:
2163 1123 243 137
SacI G_AGCT'C
Cuts at: 0 698 3666
Size: 698 2968
SapI GCTCTTCn'nnn_
Cuts at: 0 703 3666
Size: 703 2963
ScaI AGT'ACT
Cuts at: 0 1491 3666
Size: 1491 2175
SfcI C'TryA_G
Cuts at: 0 983 1317 2582 2772 2894 3666
Size: 983 334 1265 190 122 772
Fragments arranged by size:
1265 983 772 334 190 122
SmlI C'TyrA_G
Cuts at: 0 711 2371 3666
Size: 711 1660 1295
Fragments arranged by size:
1660 1295 711
SphI G_CATG'C
Cuts at: 0 667 709 3666
Size: 667 42 2957
Fragments arranged by size:
2957 667 42
SspI AAT'ATT
Cuts at: 0 2157 3666
Size: 2157 1509
StyI C'CwwG_G
Cuts at: 0 77 851 1070 1623 2288 2360 2792 3666
Size: 77 774 219 553 665 72 432 874
Fragments arranged by size:
874 774 665 553 432 219 77 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 342 478 3666
Size: 280 62 136 3188
Fragments arranged by size:
3188 280 136 62
TatI w'GTAC_w
Cuts at: 0 1160 1489 2678 2818 3382 3666
Size: 1160 329 1189 140 564 284
Fragments arranged by size:
1189 1160 564 329 284 140
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 142 174 3578 3610 3666
Size: 142 32 3404 32 56
Fragments arranged by size:
3404 142 56 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 69 3666
Size: 69 3597
XmnI GAAnn'nnTTC
Cuts at: 0 132 1689 2510 3479 3666
Size: 132 1557 821 969 187
Fragments arranged by size:
1557 969 821 187 132
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AleI AlfI AlwNI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BciVI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
Hin4I HincII HindIII HpaI KpnI MmeI MslI MspA1I
NcoI NdeI NspI PciI PflMI PfoI PpuMI PshAI
PsrI PstI PvuII SacI SapI ScaI SfcI SmlI
SphI SspI StyI TaqII TatI TstI Tth111I XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AloI ApaI
AscI AsiSI AvrII BaeI BbeI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI
BsiEI BsiWI BspHI BsrFI BsrGI BssHII BstZ17I BtgZI
ClaI CspCI DraI EagI EciI EcoNI FalI FseI
FspI FspAI HaeII KasI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI XbaI
XcmI XhoI XmaI ZraI