Restriction map for
pFN21AE3488
(Linear) (Six-base) MAPSORT of: KIEE3488.seq Check: 7087 from: 1 to: 5478
KIEE3488.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AccI GT'mk_AC
Cuts at: 0 320 5478
Size: 320 5158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1681 2770 3353 5285 5321 5422 5432 5478
Size: 1681 1089 583 1932 36 101 10 46
Fragments arranged by size:
1932 1681 1089 583 101 46 36 10
AflII C'TTAA_G
Cuts at: 0 2647 3523 3757 3784 5478
Size: 2647 876 234 27 1694
Fragments arranged by size:
2647 1694 876 234 27
AflIII A'CryG_T
Cuts at: 0 1721 3374 5478
Size: 1721 1653 2104
Fragments arranged by size:
2104 1721 1653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 478 728 1433 2729 5478
Size: 478 250 705 1296 2749
Fragments arranged by size:
2749 1296 705 478 250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4124 4156 5478
Size: 4124 32 1322
Fragments arranged by size:
4124 1322 32
AlwNI CAG_nnn'CTG
Cuts at: 0 628 664 2335 5478
Size: 628 36 1671 3143
Fragments arranged by size:
3143 1671 628 36
ApaI G_GGCC'C
Cuts at: 0 1883 3185 4979 5478
Size: 1883 1302 1794 499
Fragments arranged by size:
1883 1794 1302 499
ApaLI G'TGCA_C
Cuts at: 0 1934 5478
Size: 1934 3544
ApoI r'AATT_y
Cuts at: 0 754 1015 1207 1592 1673 3082 3193 3667
Size: 754 261 192 385 81 1409 111 474
Cuts at: 3667 3679 3886 5478
Size: 12 207 1592
Fragments arranged by size:
1592 1409 754 474 385 261 207 192
111 81 12
AvaI C'yCGr_G
Cuts at: 0 483 4379 5033 5478
Size: 483 3896 654 445
Fragments arranged by size:
3896 654 483 445
BanI G'GyrC_C
Cuts at: 0 271 1801 3859 5478
Size: 271 1530 2058 1619
Fragments arranged by size:
2058 1619 1530 271
BanII G_rGCy'C
Cuts at: 0 1883 2254 3185 4979 5478
Size: 1883 371 931 1794 499
Fragments arranged by size:
1883 1794 931 499 371
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 551 1786 3019 3521 4020 4517 5147
Size: 449 102 1235 1233 502 499 497 630
Cuts at: 5147 5478
Size: 331
Fragments arranged by size:
1235 1233 630 502 499 497 449 331
102
BbvCI CC'TCA_GC
Cuts at: 0 25 5478
Size: 25 5453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 1885 1919 4045 4079 5478
Size: 27 1858 34 2126 34 1399
Fragments arranged by size:
2126 1858 1399 34 34 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 127 2522 5478
Size: 127 2395 2956
Fragments arranged by size:
2956 2395 127
BclI T'GATC_A
Cuts at: 0 2345 5478
Size: 2345 3133
BfrBI ATG'CAT
Cuts at: 0 2460 5478
Size: 2460 3018
BglI GCCn_nnn'nGGC
Cuts at: 0 1358 5478
Size: 1358 4120
BglII A'GATC_T
Cuts at: 0 2085 4248 4460 5478
Size: 2085 2163 212 1018
Fragments arranged by size:
2163 2085 1018 212
BlpI GC'TnA_GC
Cuts at: 0 3224 3468 5478
Size: 3224 244 2010
Fragments arranged by size:
3224 2010 244
Bme1580I G_kGCm'C
Cuts at: 0 1804 1883 1938 3185 4979 5471 5478
Size: 1804 79 55 1247 1794 492 7
Fragments arranged by size:
1804 1794 1247 492 79 55 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 269 2918 4642 5478
Size: 269 2649 1724 836
Fragments arranged by size:
2649 1724 836 269
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1635 5160 5478
Size: 1635 3525 318
Fragments arranged by size:
3525 1635 318
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 172 1052 2213 2291 2427 3194 4243
Size: 110 62 880 1161 78 136 767 1049
Cuts at: 4243 4262 4423 5081 5186 5478
Size: 19 161 658 105 292
Fragments arranged by size:
1161 1049 880 767 658 292 161 136
110 105 78 62 19
Bpu10I CC'TnA_GC
Cuts at: 0 25 1520 2690 4835 5195 5478
Size: 25 1495 1170 2145 360 283
Fragments arranged by size:
2145 1495 1170 360 283 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1240 2305 3535 4069 4918 5478
Size: 1240 1065 1230 534 849 560
Fragments arranged by size:
1240 1230 1065 849 560 534
BsaI GGTCTCn'nnnn_
Cuts at: 0 4702 5478
Size: 4702 776
BsaAI yAC'GTr
Cuts at: 0 3079 3412 4717 5478
Size: 3079 333 1305 761
Fragments arranged by size:
3079 1305 761 333
BsaBI GATnn'nnATC
Cuts at: 0 2084 5225 5478
Size: 2084 3141 253
Fragments arranged by size:
3141 2084 253
BsaHI Gr'CG_yC
Cuts at: 0 4185 5478
Size: 4185 1293
BsaWI w'CCGG_w
Cuts at: 0 2582 5478
Size: 2582 2896
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1615 1645 3946 3976 4328 4358 5079 5109
Size: 1615 30 2301 30 352 30 721 30
Cuts at: 5109 5478
Size: 369
Fragments arranged by size:
2301 1615 721 369 352 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 458 3436 3970 3973 5191 5194 5478
Size: 458 2978 534 3 1218 3 284
Fragments arranged by size:
2978 1218 534 458 284 3 3
BseYI C'CCAG_C
Cuts at: 0 216 694 1113 1493 3122 5130 5326 5478
Size: 216 478 419 380 1629 2008 196 152
Fragments arranged by size:
2008 1629 478 419 380 216 196 152
BsiHKAI G_wGCw'C
Cuts at: 0 834 1938 2199 2307 5478
Size: 834 1104 261 108 3171
Fragments arranged by size:
3171 1104 834 261 108
BsmI GAATG_Cn'
Cuts at: 0 898 1827 3455 5478
Size: 898 929 1628 2023
Fragments arranged by size:
2023 1628 929 898
BsmBI CGTCTCn'nnnn_
Cuts at: 0 5036 5478
Size: 5036 442
Bsp1286I G_dGCh'C
Cuts at: 0 834 1804 1883 1938 2199 2254 2307 3185
Size: 834 970 79 55 261 55 53 878
Cuts at: 3185 4979 5471 5478
Size: 1794 492 7
Fragments arranged by size:
1794 970 878 834 492 261 79 55
55 53 7
BspEI T'CCGG_A
Cuts at: 0 2582 5478
Size: 2582 2896
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2124 5478
Size: 2124 3354
BsrBI CCG'CTC
Cuts at: 0 142 3432 5478
Size: 142 3290 2046
Fragments arranged by size:
3290 2046 142
BsrDI GCAATG_nn'
Cuts at: 0 566 1236 2870 5478
Size: 566 670 1634 2608
Fragments arranged by size:
2608 1634 670 566
BsrGI T'GTAC_A
Cuts at: 0 4912 5253 5478
Size: 4912 341 225
Fragments arranged by size:
4912 341 225
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3859 5478
Size: 3859 1619
BstBI TT'CG_AA
Cuts at: 0 3925 5478
Size: 3925 1553
BstXI CCAn_nnnn'nTGG
Cuts at: 0 821 1030 1875 4631 5478
Size: 821 209 845 2756 847
Fragments arranged by size:
2756 847 845 821 209
BstYI r'GATC_y
Cuts at: 0 878 1476 1743 2064 2085 2187 2919 4248
Size: 878 598 267 321 21 102 732 1329
Cuts at: 4248 4460 4479 5478
Size: 212 19 999
Fragments arranged by size:
1329 999 878 732 598 321 267 212
102 21 19
Bsu36I CC'TnA_GG
Cuts at: 0 641 1340 2025 2060 3095 5478
Size: 641 699 685 35 1035 2383
Fragments arranged by size:
2383 1035 699 685 641 35
BtgI C'CryG_G
Cuts at: 0 1747 2521 3201 5478
Size: 1747 774 680 2277
Fragments arranged by size:
2277 1747 774 680
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1888 5478
Size: 1888 3590
BtsI GCAGTG_nn'
Cuts at: 0 44 973 1140 2416 2713 3011 5087 5478
Size: 44 929 167 1276 297 298 2076 391
Fragments arranged by size:
2076 1276 929 391 298 297 167 44
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 117 152 5478
Size: 117 35 5326
Fragments arranged by size:
5326 117 35
DraIII CAC_nnn'GTG
Cuts at: 0 2930 4348 5478
Size: 2930 1418 1130
Fragments arranged by size:
2930 1418 1130
DrdI GACnn_nn'nnGTC
Cuts at: 0 4597 4814 5478
Size: 4597 217 664
Fragments arranged by size:
4597 664 217
EaeI y'GGCC_r
Cuts at: 0 2265 2403 2518 4324 4633 5478
Size: 2265 138 115 1806 309 845
Fragments arranged by size:
2265 1806 845 309 138 115
EarI CTCTTCn'nnn_
Cuts at: 0 866 1604 1660 2500 2581 2672 3095 3572
Size: 866 738 56 840 81 91 423 477
Cuts at: 3572 3704 3941 4379 5234 5478
Size: 132 237 438 855 244
Fragments arranged by size:
866 855 840 738 477 438 423 244
237 132 91 81 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 840 3744 5478
Size: 840 2904 1734
Fragments arranged by size:
2904 1734 840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 172 1052 1681 2213 2291 2427 2770
Size: 110 62 880 629 532 78 136 343
Cuts at: 2770 3194 3353 4243 4262 4423 5081 5186 5285
Size: 424 159 890 19 161 658 105 99
Cuts at: 5285 5321 5422 5432 5478
Size: 36 101 10 46
Fragments arranged by size:
890 880 658 629 532 424 343 161
159 136 110 105 101 99 78 62
46 36 19 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 5281 5478
Size: 5281 197
EcoO109I rG'GnC_Cy
Cuts at: 0 637 1879 1951 2864 3181 3790 4975 4976
Size: 637 1242 72 913 317 609 1185 1
Cuts at: 4976 5478
Size: 502
Fragments arranged by size:
1242 1185 913 637 609 502 317 72
1
EcoRI G'AATT_C
Cuts at: 0 1673 5478
Size: 1673 3805
EcoRV GAT'ATC
Cuts at: 0 1317 5478
Size: 1317 4161
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 207 5478
Size: 207 5271
FspI TGC'GCA
Cuts at: 0 2053 3145 3643 5478
Size: 2053 1092 498 1835
Fragments arranged by size:
2053 1835 1092 498
FspAI rTGC'GCAy
Cuts at: 0 2053 3145 3643 5478
Size: 2053 1092 498 1835
Fragments arranged by size:
2053 1835 1092 498
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 453 485 533 565 610 624 642 656
Size: 453 32 48 32 45 14 18 14
Cuts at: 656 1323 1355 2499 2531 3289 3310 3321 3342
Size: 667 32 1144 32 758 21 11 21
Cuts at: 3342 3485 3517 3533 3565 4677 4709 5478
Size: 143 32 16 32 1112 32 769
Fragments arranged by size:
1144 1112 769 758 667 453 143 48
45 32 32 32 32 32 32 32
21 21 18 16 14 14 11
HincII GTy'rAC
Cuts at: 0 3155 5478
Size: 3155 2323
HindIII A'AGCT_T
Cuts at: 0 3781 5478
Size: 3781 1697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1520 1989 3944 4110 5478
Size: 1520 469 1955 166 1368
Fragments arranged by size:
1955 1520 1368 469 166
MscI TGG'CCA
Cuts at: 0 2267 2405 4635 5478
Size: 2267 138 2230 843
Fragments arranged by size:
2267 2230 843 138
MslI CAynn'nnrTG
Cuts at: 0 994 1873 2623 3414 3494 5462 5478
Size: 994 879 750 791 80 1968 16
Fragments arranged by size:
1968 994 879 791 750 80 16
MspA1I CmG'CkG
Cuts at: 0 150 1278 3122 3331 3405 3622 5370 5478
Size: 150 1128 1844 209 74 217 1748 108
Fragments arranged by size:
1844 1748 1128 217 209 150 108 74
NcoI C'CATG_G
Cuts at: 0 1747 5478
Size: 1747 3731
NdeI CA'TA_TG
Cuts at: 0 3872 5478
Size: 3872 1606
NsiI A_TGCA'T
Cuts at: 0 2462 5478
Size: 2462 3016
NspI r_CATG'y
Cuts at: 0 1725 3378 3857 5446 5478
Size: 1725 1653 479 1589 32
Fragments arranged by size:
1725 1653 1589 479 32
PasI CC'CwG_GG
Cuts at: 0 4972 5478
Size: 4972 506
PciI A'CATG_T
Cuts at: 0 1721 3374 5478
Size: 1721 1653 2104
Fragments arranged by size:
2104 1721 1653
PflMI CCAn_nnn'nTGG
Cuts at: 0 694 888 5478
Size: 694 194 4590
Fragments arranged by size:
4590 694 194
PfoI T'CCnGG_A
Cuts at: 0 881 2190 4220 5478
Size: 881 1309 2030 1258
Fragments arranged by size:
2030 1309 1258 881
PmlI CAC'GTG
Cuts at: 0 3412 5478
Size: 3412 2066
PpuMI rG'GwC_Cy
Cuts at: 0 637 1951 3790 5478
Size: 637 1314 1839 1688
Fragments arranged by size:
1839 1688 1314 637
PshAI GACnn'nnGTC
Cuts at: 0 3041 5478
Size: 3041 2437
PsiI TTA'TAA
Cuts at: 0 2168 5478
Size: 2168 3310
PspOMI G'GGCC_C
Cuts at: 0 1879 3181 4975 5478
Size: 1879 1302 1794 503
Fragments arranged by size:
1879 1794 1302 503
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4840 4872 5478
Size: 4840 32 606
Fragments arranged by size:
4840 606 32
PstI C_TGCA'G
Cuts at: 0 2279 5478
Size: 2279 3199
PvuII CAG'CTG
Cuts at: 0 150 1278 3331 3405 3622 5370 5478
Size: 150 1128 2053 74 217 1748 108
Fragments arranged by size:
2053 1748 1128 217 150 108 74
SapI GCTCTTCn'nnn_
Cuts at: 0 866 3941 5478
Size: 866 3075 1537
Fragments arranged by size:
3075 1537 866
ScaI AGT'ACT
Cuts at: 0 1924 5478
Size: 1924 3554
SfcI C'TryA_G
Cuts at: 0 317 2275 2476 3271 5478
Size: 317 1958 201 795 2207
Fragments arranged by size:
2207 1958 795 317 201
SmaI CCC'GGG
Cuts at: 0 485 5478
Size: 485 4993
SmlI C'TyrA_G
Cuts at: 0 1219 2284 2647 3523 3550 3757 3784 4084
Size: 1219 1065 363 876 27 207 27 300
Cuts at: 4084 4897 5033 5478
Size: 813 136 445
Fragments arranged by size:
1219 1065 876 813 445 363 300 207
136 27 27
SnaBI TAC'GTA
Cuts at: 0 3079 4717 5478
Size: 3079 1638 761
Fragments arranged by size:
3079 1638 761
SphI G_CATG'C
Cuts at: 0 5446 5478
Size: 5446 32
SspI AAT'ATT
Cuts at: 0 1818 5478
Size: 1818 3660
StyI C'CwwG_G
Cuts at: 0 275 1444 1634 1747 2848 5121 5478
Size: 275 1169 190 113 1101 2273 357
Fragments arranged by size:
2273 1169 1101 357 275 190 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1105 1437 1885 2631 4618 4661 5122 5478
Size: 1105 332 448 746 1987 43 461 356
Fragments arranged by size:
1987 1105 746 461 448 356 332 43
TatI w'GTAC_w
Cuts at: 0 304 1922 4820 4912 5253 5478
Size: 304 1618 2898 92 341 225
Fragments arranged by size:
2898 1618 341 304 225 92
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 255 287 4253 4285 5079 5111
Size: 163 32 60 32 3966 32 794 32
Cuts at: 5111 5478
Size: 367
Fragments arranged by size:
3966 794 367 163 60 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3545 4209 5478
Size: 3545 664 1269
Fragments arranged by size:
3545 1269 664
XbaI T'CTAG_A
Cuts at: 0 1192 4761 5478
Size: 1192 3569 717
Fragments arranged by size:
3569 1192 717
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1065 5478
Size: 1065 4413
XhoI C'TCGA_G
Cuts at: 0 5033 5478
Size: 5033 445
XmaI C'CCGG_G
Cuts at: 0 483 5478
Size: 483 4995
XmnI GAAnn'nnTTC
Cuts at: 0 746 5478
Size: 746 4732
Enzymes that do cut:
AccI AcuI AflII AflIII AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrGI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI FspAI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PasI PciI
PflMI PfoI PmlI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuII SapI ScaI SfcI SmaI SmlI SnaBI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BmgBI
BmtI BsgI BsiEI BsiWI BspHI BsrFI BssHII BssSI
BstEII BstZ17I ClaI DraI EagI EcoICRI FseI HaeII
HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV
NheI NotI NruI PacI PmeI PpiI PspXI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SpeI SrfI StuI SwaI ZraI