Restriction map for
pFN21AE3476
(Linear) (Six-base) MAPSORT of: KIEE3476.seq Check: 426 from: 1 to: 4653
KIEE3476.orf
With 182 enzymes: SgfI *
July 3, 2012 17:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 890 1178 1689 3165 3496 3681 4653
Size: 890 288 511 1476 331 185 972
Fragments arranged by size:
1476 972 890 511 331 288 185
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 1552 4300 4653
Size: 343 1209 2748 353
Fragments arranged by size:
2748 1209 353 343
AfeI AGC'GCT
Cuts at: 0 2243 4653
Size: 2243 2410
AflIII A'CryG_T
Cuts at: 0 4341 4653
Size: 4341 312
AhdI GACnn_n'nnGTC
Cuts at: 0 777 2672 4653
Size: 777 1895 1981
Fragments arranged by size:
1981 1895 777
AleI CACnn'nnGTG
Cuts at: 0 259 3577 4653
Size: 259 3318 1076
Fragments arranged by size:
3318 1076 259
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 305 3148 3595 3689 4291 4653
Size: 305 2843 447 94 602 362
Fragments arranged by size:
2843 602 447 362 305 94
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2998 3030 4653
Size: 2998 32 1623
Fragments arranged by size:
2998 1623 32
AlwNI CAG_nnn'CTG
Cuts at: 0 150 945 1263 1365 1774 1995 2100 2274
Size: 150 795 318 102 409 221 105 174
Cuts at: 2274 2358 2718 2842 3180 3198 3405 3543 3690
Size: 84 360 124 338 18 207 138 147
Cuts at: 3690 4653
Size: 963
Fragments arranged by size:
963 795 409 360 338 318 221 207
174 150 147 138 124 105 102 84
18
ApaI G_GGCC'C
Cuts at: 0 1076 1427 1545 1626 2400 2982 3628 3634
Size: 1076 351 118 81 774 582 646 6
Cuts at: 3634 3641 4351 4653
Size: 7 710 302
Fragments arranged by size:
1076 774 710 646 582 351 302 118
81 7 6
ApaLI G'TGCA_C
Cuts at: 0 3123 4653
Size: 3123 1530
AvaI C'yCGr_G
Cuts at: 0 57 505 1619 1649 2399 3520 3527 3627
Size: 57 448 1114 30 750 1121 7 100
Cuts at: 3627 4653
Size: 1026
Fragments arranged by size:
1121 1114 1026 750 448 100 57 30
7
BanI G'GyrC_C
Cuts at: 0 2146 2865 3325 3508 3669 3721 3792 3841
Size: 2146 719 460 183 161 52 71 49
Cuts at: 3841 4105 4193 4369 4463 4653
Size: 264 88 176 94 190
Fragments arranged by size:
2146 719 460 264 190 183 176 161
94 88 71 52 49
BanII G_rGCy'C
Cuts at: 0 65 107 1076 1427 1535 1545 1626 1633
Size: 65 42 969 351 108 10 81 7
Cuts at: 1633 2372 2400 2461 2982 3341 3418 3628 3634
Size: 739 28 61 521 359 77 210 6
Cuts at: 3634 3641 4090 4351 4579 4648 4653
Size: 7 449 261 228 69 5
Fragments arranged by size:
969 739 521 449 359 351 261 228
210 108 81 77 69 65 61 42
28 10 7 7 6 5
BbeI G_GCGC'C
Cuts at: 0 3725 4197 4373 4467 4653
Size: 3725 472 176 94 186
Fragments arranged by size:
3725 472 186 176 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 2605 4653
Size: 2605 2048
BbvCI CC'TCA_GC
Cuts at: 0 1390 2053 3859 4653
Size: 1390 663 1806 794
Fragments arranged by size:
1806 1390 794 663
BglI GCCn_nnn'nGGC
Cuts at: 0 758 2714 2767 4653
Size: 758 1956 53 1886
Fragments arranged by size:
1956 1886 758 53
BglII A'GATC_T
Cuts at: 0 78 215 2526 2997 4653
Size: 78 137 2311 471 1656
Fragments arranged by size:
2311 1656 471 137 78
BlpI GC'TnA_GC
Cuts at: 0 106 478 1285 1933 2318 2621 2962 3853
Size: 106 372 807 648 385 303 341 891
Cuts at: 3853 4573 4653
Size: 720 80
Fragments arranged by size:
891 807 720 648 385 372 341 303
106 80
Bme1580I G_kGCm'C
Cuts at: 0 623 1076 1427 1545 1626 2400 2763 2789
Size: 623 453 351 118 81 774 363 26
Cuts at: 2789 2982 3127 3628 3634 3641 4351 4653
Size: 193 145 501 6 7 710 302
Fragments arranged by size:
774 710 623 501 453 363 351 302
193 145 118 81 26 7 6
BmgBI CAC'GTC
Cuts at: 0 900 909 4023 4653
Size: 900 9 3114 630
Fragments arranged by size:
3114 900 630 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 287 510 1032 2037 2966 3006 3413 3847
Size: 287 223 522 1005 929 40 407 434
Cuts at: 3847 4653
Size: 806
Fragments arranged by size:
1005 929 806 522 434 407 287 223
40
BmtI G_CTAG'C
Cuts at: 0 1613 4653
Size: 1613 3040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1239 2094 4653
Size: 1239 855 2559
Fragments arranged by size:
2559 1239 855
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1240 1264 1285 1357 1660 1690 1879
Size: 43 1197 24 21 72 303 30 189
Cuts at: 1879 2017 2146 2248 2266 3508 4653
Size: 138 129 102 18 1242 1145
Fragments arranged by size:
1242 1197 1145 303 189 138 129 102
72 43 30 24 21 18
Bpu10I CC'TnA_GC
Cuts at: 0 151 1061 1390 1520 1627 1690 2053 3859
Size: 151 910 329 130 107 63 363 1806
Cuts at: 3859 4653
Size: 794
Fragments arranged by size:
1806 910 794 363 329 151 130 107
63
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1756 2064 2154 3226 3432 4042 4653
Size: 1756 308 90 1072 206 610 611
Fragments arranged by size:
1756 1072 611 610 308 206 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 1771 2082 4605 4653
Size: 1771 311 2523 48
Fragments arranged by size:
2523 1771 311 48
BsaAI yAC'GTr
Cuts at: 0 3122 3900 4344 4653
Size: 3122 778 444 309
Fragments arranged by size:
3122 778 444 309
BsaHI Gr'CG_yC
Cuts at: 0 700 820 2945 3722 4194 4370 4464 4653
Size: 700 120 2125 777 472 176 94 189
Fragments arranged by size:
2125 777 700 472 189 176 120 94
BsaWI w'CCGG_w
Cuts at: 0 992 1925 2453 4653
Size: 992 933 528 2200
Fragments arranged by size:
2200 992 933 528
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 628 658 697 727 1888 1918
Size: 415 30 183 30 39 30 1161 30
Cuts at: 1918 2113 2143 2221 2251 4432 4462 4653
Size: 195 30 78 30 2181 30 191
Fragments arranged by size:
2181 1161 415 195 191 183 78 39
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 251 367 436 754 1573 1753 1909 2134
Size: 251 116 69 318 819 180 156 225
Cuts at: 2134 2401 2531 2788 3346 3814 4041 4261 4653
Size: 267 130 257 558 468 227 220 392
Fragments arranged by size:
819 558 468 392 318 267 257 251
227 225 220 180 156 130 116 69
BseYI C'CCAG_C
Cuts at: 0 155 564 1428 1634 2036 2057 2306 2697
Size: 155 409 864 206 402 21 249 391
Cuts at: 2697 3098 3180 3457 3621 3699 3777 3962 4467
Size: 401 82 277 164 78 78 185 505
Cuts at: 4467 4653
Size: 186
Fragments arranged by size:
864 505 409 402 401 391 277 249
206 186 185 164 155 82 78 78
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 289 385 541 1294 1378 2317 2667
Size: 79 210 96 156 753 84 939 350
Cuts at: 2667 3418 3443 3703 4063 4653
Size: 751 25 260 360 590
Fragments arranged by size:
939 753 751 590 360 350 260 210
156 96 84 79 25
BsiEI CG_ry'CG
Cuts at: 0 234 3708 4653
Size: 234 3474 945
Fragments arranged by size:
3474 945 234
BsiHKAI G_wGCw'C
Cuts at: 0 107 368 1535 1633 2372 2461 2583 3127
Size: 107 261 1167 98 739 89 122 544
Cuts at: 3127 3584 3611 4648 4653
Size: 457 27 1037 5
Fragments arranged by size:
1167 1037 739 544 457 261 122 107
98 89 27 5
BsmBI CGTCTCn'nnnn_
Cuts at: 0 502 661 811 2008 3472 4493 4653
Size: 502 159 150 1197 1464 1021 160
Fragments arranged by size:
1464 1197 1021 502 160 159 150
Bsp1286I G_dGCh'C
Cuts at: 0 65 107 368 623 1076 1427 1535 1545
Size: 65 42 261 255 453 351 108 10
Cuts at: 1545 1626 1633 2372 2400 2461 2583 2763 2789
Size: 81 7 739 28 61 122 180 26
Cuts at: 2789 2982 3127 3341 3418 3584 3611 3628 3634
Size: 193 145 214 77 166 27 17 6
Cuts at: 3634 3641 4090 4351 4579 4648 4653
Size: 7 449 261 228 69 5
Fragments arranged by size:
739 453 449 351 261 261 255 228
214 193 180 166 145 122 108 81
77 69 65 61 42 28 27 26
17 10 7 7 6 5
BspEI T'CCGG_A
Cuts at: 0 992 2453 4653
Size: 992 1461 2200
Fragments arranged by size:
2200 1461 992
BspHI T'CATG_A
Cuts at: 0 863 4653
Size: 863 3790
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 890 1178 1689 2099 2979 3077 3165 3496
Size: 890 288 511 410 880 98 88 331
Cuts at: 3496 3681 4587 4653
Size: 185 906 66
Fragments arranged by size:
906 890 880 511 410 331 288 185
98 88 66
BsrBI CCG'CTC
Cuts at: 0 6 357 963 1029 1248 1950 2007 2283
Size: 6 351 606 66 219 702 57 276
Cuts at: 2283 3159 4056 4653
Size: 876 897 597
Fragments arranged by size:
897 876 702 606 597 351 276 219
66 57 6
BsrFI r'CCGG_y
Cuts at: 0 46 1694 3389 3707 3727 3784 4190 4653
Size: 46 1648 1695 318 20 57 406 463
Fragments arranged by size:
1695 1648 463 406 318 57 46 20
BsrGI T'GTAC_A
Cuts at: 0 3233 4653
Size: 3233 1420
BssHII G'CGCG_C
Cuts at: 0 15 17 4653
Size: 15 2 4636
Fragments arranged by size:
4636 15 2
BssSI C'ACGA_G
Cuts at: 0 100 367 871 3923 3931 4653
Size: 100 267 504 3052 8 722
Fragments arranged by size:
3052 722 504 267 100 8
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1365 2274 2587 3198 3841 4050 4653
Size: 1365 909 313 611 643 209 603
Fragments arranged by size:
1365 909 643 611 603 313 209
BstBI TT'CG_AA
Cuts at: 0 1010 4653
Size: 1010 3643
BstEII G'GTnAC_C
Cuts at: 0 252 390 4653
Size: 252 138 4263
Fragments arranged by size:
4263 252 138
BstXI CCAn_nnnn'nTGG
Cuts at: 0 672 1420 1788 2112 4653
Size: 672 748 368 324 2541
Fragments arranged by size:
2541 748 672 368 324
BstYI r'GATC_y
Cuts at: 0 78 215 722 2255 2526 2997 3515 4653
Size: 78 137 507 1533 271 471 518 1138
Fragments arranged by size:
1533 1138 518 507 471 271 137 78
Bsu36I CC'TnA_GG
Cuts at: 0 1197 1799 1811 2877 2956 3797 4132 4653
Size: 1197 602 12 1066 79 841 335 521
Fragments arranged by size:
1197 1066 841 602 521 335 79 12
BtgI C'CryG_G
Cuts at: 0 257 734 972 1076 1146 2869 3450 3816
Size: 257 477 238 104 70 1723 581 366
Cuts at: 3816 3845 3956 4416 4475 4653
Size: 29 111 460 59 178
Fragments arranged by size:
1723 581 477 460 366 257 238 178
111 104 70 59 29
BtsI GCAGTG_nn'
Cuts at: 0 2585 2840 3113 3884 4653
Size: 2585 255 273 771 769
Fragments arranged by size:
2585 771 769 273 255
ClaI AT'CG_AT
Cuts at: 0 4010 4653
Size: 4010 643
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3906 3941 4653
Size: 3906 35 712
Fragments arranged by size:
3906 712 35
DraIII CAC_nnn'GTG
Cuts at: 0 769 3122 3841 4653
Size: 769 2353 719 812
Fragments arranged by size:
2353 812 769 719
DrdI GACnn_nn'nnGTC
Cuts at: 0 4536 4653
Size: 4536 117
EaeI y'GGCC_r
Cuts at: 0 231 542 1161 1493 1601 1838 2102 2167
Size: 231 311 619 332 108 237 264 65
Cuts at: 2167 2219 3214 3447 3927 3943 4653
Size: 52 995 233 480 16 710
Fragments arranged by size:
995 710 619 480 332 311 264 237
233 231 108 65 52 16
EagI C'GGCC_G
Cuts at: 0 231 4653
Size: 231 4422
EarI CTCTTCn'nnn_
Cuts at: 0 319 953 3883 4391 4510 4653
Size: 319 634 2930 508 119 143
Fragments arranged by size:
2930 634 508 319 143 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 925 3496 4108 4653
Size: 925 2571 612 545
Fragments arranged by size:
2571 925 612 545
EcoICRI GAG'CTC
Cuts at: 0 105 1533 1631 2370 2459 4646 4653
Size: 105 1428 98 739 89 2187 7
Fragments arranged by size:
2187 1428 739 105 98 89 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 343 1240 1264 1285 1357 1552 1660
Size: 43 300 897 24 21 72 195 108
Cuts at: 1660 1690 1879 2017 2146 2248 2266 3508 4300
Size: 30 189 138 129 102 18 1242 792
Cuts at: 4300 4653
Size: 353
Fragments arranged by size:
1242 897 792 353 300 195 189 138
129 108 102 72 43 30 24 21
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1462 2220 2362 2986 3304 3829 4131 4594
Size: 1462 758 142 624 318 525 302 463
Cuts at: 4594 4653
Size: 59
Fragments arranged by size:
1462 758 624 525 463 318 302 142
59
EcoO109I rG'GnC_Cy
Cuts at: 0 462 1072 1127 1151 1305 1424 1541 1623
Size: 462 610 55 24 154 119 117 82
Cuts at: 1623 1792 2097 2271 2355 2481 2715 2769 2978
Size: 169 305 174 84 126 234 54 209
Cuts at: 2978 2979 2991 3006 3066 3631 3638 3822 4284
Size: 1 12 15 60 565 7 184 462
Cuts at: 4284 4348 4653
Size: 64 305
Fragments arranged by size:
610 565 462 462 305 305 234 209
184 174 169 154 126 119 117 84
82 64 60 55 54 24 15 12
7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4242 4653
Size: 4242 411
FspI TGC'GCA
Cuts at: 0 2557 3358 4653
Size: 2557 801 1295
Fragments arranged by size:
2557 1295 801
HaeII r_GCGC'y
Cuts at: 0 94 923 2245 2325 3603 3651 3725 3778
Size: 94 829 1322 80 1278 48 74 53
Cuts at: 3778 3955 4197 4224 4373 4467 4653
Size: 177 242 27 149 94 186
Fragments arranged by size:
1322 1278 829 242 186 177 149 94
94 80 74 53 48 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 628 660 697 729 763 795 1387 1419
Size: 628 32 37 32 34 32 592 32
Cuts at: 1419 1729 1761 2113 2145 2221 2253 2658 2690
Size: 310 32 352 32 76 32 405 32
Cuts at: 2690 4537 4569 4653
Size: 1847 32 84
Fragments arranged by size:
1847 628 592 405 352 310 84 76
37 34 32 32 32 32 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 3721 4193 4369 4463 4653
Size: 3721 472 176 94 190
Fragments arranged by size:
3721 472 190 176 94
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 4223 4653
Size: 1112 3111 430
Fragments arranged by size:
3111 1112 430
MscI TGG'CCA
Cuts at: 0 544 2104 3929 3945 4653
Size: 544 1560 1825 16 708
Fragments arranged by size:
1825 1560 708 544 16
MslI CAynn'nnrTG
Cuts at: 0 259 298 581 2110 3577 3898 4012 4653
Size: 259 39 283 1529 1467 321 114 641
Fragments arranged by size:
1529 1467 641 321 283 259 114 39
MspA1I CmG'CkG
Cuts at: 0 30 113 564 942 1432 1638 1710 1857
Size: 30 83 451 378 490 206 72 147
Cuts at: 1857 1911 1992 2057 2310 2681 2871 3102 3195
Size: 54 81 65 253 371 190 231 93
Cuts at: 3195 3270 3405 3452 3550 3621 3750 3818 4143
Size: 75 135 47 98 71 129 68 325
Cuts at: 4143 4200 4418 4434 4653
Size: 57 218 16 219
Fragments arranged by size:
490 451 378 371 325 253 231 219
218 206 190 147 135 129 98 93
83 81 75 72 71 68 65 57
54 47 30 16
NaeI GCC'GGC
Cuts at: 0 48 1696 3391 4192 4653
Size: 48 1648 1695 801 461
Fragments arranged by size:
1695 1648 801 461 48
NarI GG'CG_CC
Cuts at: 0 3722 4194 4370 4464 4653
Size: 3722 472 176 94 189
Fragments arranged by size:
3722 472 189 176 94
NcoI C'CATG_G
Cuts at: 0 734 1076 3956 4475 4653
Size: 734 342 2880 519 178
Fragments arranged by size:
2880 734 519 342 178
NgoMIV G'CCGG_C
Cuts at: 0 46 1694 3389 4190 4653
Size: 46 1648 1695 801 463
Fragments arranged by size:
1695 1648 801 463 46
NheI G'CTAG_C
Cuts at: 0 1609 4653
Size: 1609 3044
PasI CC'CwG_GG
Cuts at: 0 1545 2811 3634 4563 4653
Size: 1545 1266 823 929 90
Fragments arranged by size:
1545 1266 929 823 90
PflMI CCAn_nnn'nTGG
Cuts at: 0 1263 2718 3543 3962 4653
Size: 1263 1455 825 419 691
Fragments arranged by size:
1455 1263 825 691 419
PfoI T'CCnGG_A
Cuts at: 0 347 4169 4653
Size: 347 3822 484
Fragments arranged by size:
3822 484 347
PmlI CAC'GTG
Cuts at: 0 3122 4344 4653
Size: 3122 1222 309
Fragments arranged by size:
3122 1222 309
PpuMI rG'GwC_Cy
Cuts at: 0 462 1127 1792 2481 2991 3006 3066 4284
Size: 462 665 665 689 510 15 60 1218
Cuts at: 4284 4653
Size: 369
Fragments arranged by size:
1218 689 665 665 510 462 369 60
15
PspOMI G'GGCC_C
Cuts at: 0 1072 1423 1541 1622 2396 2978 3624 3630
Size: 1072 351 118 81 774 582 646 6
Cuts at: 3630 3637 4347 4653
Size: 7 710 306
Fragments arranged by size:
1072 774 710 646 582 351 306 118
81 7 6
PstI C_TGCA'G
Cuts at: 0 200 731 887 1517 1540 1715 1916 2096
Size: 200 531 156 630 23 175 201 180
Cuts at: 2096 2204 2237 2279 2315 2333 2990 3176 3194
Size: 108 33 42 36 18 657 186 18
Cuts at: 3194 3566 3668 4653
Size: 372 102 985
Fragments arranged by size:
985 657 630 531 372 201 200 186
180 175 156 108 102 42 36 33
23 18 18
PvuII CAG'CTG
Cuts at: 0 30 564 942 1432 1638 1710 1857 1911
Size: 30 534 378 490 206 72 147 54
Cuts at: 1911 2057 2310 3102 3405 3550 3621 4143 4200
Size: 146 253 792 303 145 71 522 57
Cuts at: 4200 4653
Size: 453
Fragments arranged by size:
792 534 522 490 453 378 303 253
206 147 146 145 72 71 57 54
30
SacI G_AGCT'C
Cuts at: 0 107 1535 1633 2372 2461 4648 4653
Size: 107 1428 98 739 89 2187 5
Fragments arranged by size:
2187 1428 739 107 98 89 5
SacII CC_GC'GG
Cuts at: 0 2872 3453 3819 4419 4653
Size: 2872 581 366 600 234
Fragments arranged by size:
2872 600 581 366 234
SanDI GG'GwC_CC
Cuts at: 0 1127 1792 3006 4653
Size: 1127 665 1214 1647
Fragments arranged by size:
1647 1214 1127 665
SapI GCTCTTCn'nnn_
Cuts at: 0 953 4653
Size: 953 3700
SbfI CC_TGCA'GG
Cuts at: 0 731 2096 2279 2990 3566 4653
Size: 731 1365 183 711 576 1087
Fragments arranged by size:
1365 1087 731 711 576 183
SfcI C'TryA_G
Cuts at: 0 196 727 883 1513 1536 1576 1711 1912
Size: 196 531 156 630 23 40 135 201
Cuts at: 1912 2092 2200 2233 2275 2311 2329 2603 2986
Size: 180 108 33 42 36 18 274 383
Cuts at: 2986 3172 3190 3562 3664 4653
Size: 186 18 372 102 989
Fragments arranged by size:
989 630 531 383 372 274 201 196
186 180 156 135 108 102 42 40
36 33 23 18 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 758 2714 4653
Size: 758 1956 1939
Fragments arranged by size:
1956 1939 758
SfoI GGC'GCC
Cuts at: 0 3723 4195 4371 4465 4653
Size: 3723 472 176 94 188
Fragments arranged by size:
3723 472 188 176 94
SgrAI Cr'CCGG_yG
Cuts at: 0 3727 4653
Size: 3727 926
SmaI CCC'GGG
Cuts at: 0 59 1621 1651 3629 4653
Size: 59 1562 30 1978 1024
Fragments arranged by size:
1978 1562 1024 59 30
SmlI C'TyrA_G
Cuts at: 0 1735 2079 2133 3241 3411 3527 4057 4653
Size: 1735 344 54 1108 170 116 530 596
Fragments arranged by size:
1735 1108 596 530 344 170 116 54
SrfI GCCC'GGGC
Cuts at: 0 1651 3629 4653
Size: 1651 1978 1024
Fragments arranged by size:
1978 1651 1024
StuI AGG'CCT
Cuts at: 0 1402 2215 3428 4490 4653
Size: 1402 813 1213 1062 163
Fragments arranged by size:
1402 1213 1062 813 163
StyI C'CwwG_G
Cuts at: 0 734 839 1076 2477 3956 4475 4653
Size: 734 105 237 1401 1479 519 178
Fragments arranged by size:
1479 1401 734 519 237 178 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 378 1490 2049 2164 2598 3172 3817 3829
Size: 378 1112 559 115 434 574 645 12
Cuts at: 3829 4029 4653
Size: 200 624
Fragments arranged by size:
1112 645 624 574 559 434 378 200
115 12
TatI w'GTAC_w
Cuts at: 0 2817 3233 4338 4653
Size: 2817 416 1105 315
Fragments arranged by size:
2817 1105 416 315
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1888 1920 2167 2199 4432 4464 4653
Size: 1888 32 247 32 2233 32 189
Fragments arranged by size:
2233 1888 247 189 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 598 4653
Size: 598 4055
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 672 770 1095 1882 4653
Size: 672 98 325 787 2771
Fragments arranged by size:
2771 787 672 325 98
XhoI C'TCGA_G
Cuts at: 0 3527 4653
Size: 3527 1126
XmaI C'CCGG_G
Cuts at: 0 57 1619 1649 3627 4653
Size: 57 1562 30 1978 1026
Fragments arranged by size:
1978 1562 1026 57 30
XmnI GAAnn'nnTTC
Cuts at: 0 2612 4653
Size: 2612 2041
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI
ClaI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI PasI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SapI SbfI SfcI
SfiI SfoI SgrAI SmaI SmlI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BsaBI BsiWI BsmI BsrDI BstZ17I BtgZI DraI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NdeI NotI NruI NsiI NspI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI ScaI SexAI SgfI SnaBI SpeI SphI
SspI SwaI XbaI ZraI