Restriction map for
pFN21AE3473
(Linear) (Six-base) MAPSORT of: KIEE3473.seq Check: 5091 from: 1 to: 4611
KIEE3473.orf
With 182 enzymes: SgfI *
July 3, 2012 17:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2024 4611
Size: 2024 2587
AccI GT'mk_AC
Cuts at: 0 56 77 341 4253 4611
Size: 56 21 264 3912 358
Fragments arranged by size:
3912 358 264 56 21
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 778 813 916 1897 3469 3975 4156
Size: 46 732 35 103 981 1572 506 181
Cuts at: 4156 4611
Size: 455
Fragments arranged by size:
1572 981 732 506 455 181 103 46
35
AflIII A'CryG_T
Cuts at: 0 4526 4611
Size: 4526 85
AgeI A'CCGG_T
Cuts at: 0 4382 4611
Size: 4382 229
AhdI GACnn_n'nnGTC
Cuts at: 0 2004 2730 4611
Size: 2004 726 1881
Fragments arranged by size:
2004 1881 726
AleI CACnn'nnGTG
Cuts at: 0 2018 4611
Size: 2018 2593
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 886 2771 4611
Size: 886 1885 1840
Fragments arranged by size:
1885 1840 886
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 586 618 4611
Size: 586 32 3993
Fragments arranged by size:
3993 586 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1485 1494 1539 1575 1604 1731 1875 2214
Size: 1485 9 45 36 29 127 144 339
Cuts at: 2214 3109 3411 3571 4488 4611
Size: 895 302 160 917 123
Fragments arranged by size:
1485 917 895 339 302 160 144 127
123 45 36 29 9
ApaI G_GGCC'C
Cuts at: 0 1078 3055 4024 4196 4399 4611
Size: 1078 1977 969 172 203 212
Fragments arranged by size:
1977 1078 969 212 203 172
ApaLI G'TGCA_C
Cuts at: 0 4522 4611
Size: 4522 89
AvaI C'yCGr_G
Cuts at: 0 364 397 1264 3061 3921 4024 4238 4611
Size: 364 33 867 1797 860 103 214 373
Fragments arranged by size:
1797 867 860 373 364 214 103 33
BanI G'GyrC_C
Cuts at: 0 271 862 1025 1235 1431 2029 2241 2348
Size: 271 591 163 210 196 598 212 107
Cuts at: 2348 2370 3925 4611
Size: 22 1555 686
Fragments arranged by size:
1555 686 598 591 271 212 210 196
163 107 22
BanII G_rGCy'C
Cuts at: 0 50 389 887 904 1078 1967 2125 2132
Size: 50 339 498 17 174 889 158 7
Cuts at: 2132 2165 2449 2479 2582 3055 3327 3457 3584
Size: 33 284 30 103 473 272 130 127
Cuts at: 3584 3845 4024 4045 4084 4196 4399 4611
Size: 261 179 21 39 112 203 212
Fragments arranged by size:
889 498 473 339 284 272 261 212
203 179 174 158 130 127 112 103
50 39 33 30 21 17 7
BbeI G_GCGC'C
Cuts at: 0 1029 1239 1435 2033 4611
Size: 1029 210 196 598 2578
Fragments arranged by size:
2578 1029 598 210 196
BbvCI CC'TCA_GC
Cuts at: 0 1127 1597 2341 3799 4315 4611
Size: 1127 470 744 1458 516 296
Fragments arranged by size:
1458 1127 744 516 470 296
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3229 3263 4611
Size: 3229 34 1348
Fragments arranged by size:
3229 1348 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1082 2264 2379 2686 2884 3766 3862 4234
Size: 1082 1182 115 307 198 882 96 372
Cuts at: 4234 4339 4611
Size: 105 272
Fragments arranged by size:
1182 1082 882 372 307 272 198 115
105 96
BglII A'GATC_T
Cuts at: 0 1709 1886 4611
Size: 1709 177 2725
Fragments arranged by size:
2725 1709 177
BlpI GC'TnA_GC
Cuts at: 0 44 1586 2176 2411 2473 2611 4051 4216
Size: 44 1542 590 235 62 138 1440 165
Cuts at: 4216 4611
Size: 395
Fragments arranged by size:
1542 1440 590 395 235 165 138 62
44
Bme1580I G_kGCm'C
Cuts at: 0 274 1078 2246 2375 3055 3467 3534 3928
Size: 274 804 1168 129 680 412 67 394
Cuts at: 3928 4024 4196 4399 4526 4611
Size: 96 172 203 127 85
Fragments arranged by size:
1168 804 680 412 394 274 203 172
129 127 96 85 67
BmgBI CAC'GTC
Cuts at: 0 2262 4611
Size: 2262 2349
BmrI ACTGGGnnnn_n'
Cuts at: 0 1509 1839 2202 2696 4611
Size: 1509 330 363 494 1915
Fragments arranged by size:
1915 1509 494 363 330
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 408 3585 3861 4611
Size: 408 3177 276 750
Fragments arranged by size:
3177 750 408 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 496 1774 1867 1933 2068 2095 2299
Size: 34 462 1278 93 66 135 27 204
Cuts at: 2299 2543 2889 2894 3006 3165 3433 3856 4611
Size: 244 346 5 112 159 268 423 755
Fragments arranged by size:
1278 755 462 423 346 268 244 204
159 135 112 93 66 34 27 5
Bpu10I CC'TnA_GC
Cuts at: 0 1127 1597 2341 2353 3799 4039 4102 4315
Size: 1127 470 744 12 1446 240 63 213
Cuts at: 4315 4611
Size: 296
Fragments arranged by size:
1446 1127 744 470 296 240 213 63
12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 870 1783 1960 2130 3315 3481 4243
Size: 136 734 913 177 170 1185 166 762
Cuts at: 4243 4611
Size: 368
Fragments arranged by size:
1185 913 762 734 368 177 170 166
136
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 406 580 3071 3371 3433 4611
Size: 109 297 174 2491 300 62 1178
Fragments arranged by size:
2491 1178 300 297 174 109 62
BsaAI yAC'GTr
Cuts at: 0 4527 4611
Size: 4527 84
BsaBI GATnn'nnATC
Cuts at: 0 86 4611
Size: 86 4525
BsaHI Gr'CG_yC
Cuts at: 0 551 1026 1106 1236 1432 2000 2030 2057
Size: 551 475 80 130 196 568 30 27
Cuts at: 2057 3286 4088 4342 4363 4611
Size: 1229 802 254 21 248
Fragments arranged by size:
1229 802 568 551 475 254 248 196
130 80 30 27 21
BsaWI w'CCGG_w
Cuts at: 0 4382 4611
Size: 4382 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1840 1870 2272 2302 3001 3031 3070 3100
Size: 1840 30 402 30 699 30 39 30
Cuts at: 3100 3367 3397 4441 4471 4611
Size: 267 30 1044 30 140
Fragments arranged by size:
1840 1044 699 402 267 140 39 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1329 1585 1636 1828 2706 3009 3141 3574
Size: 1329 256 51 192 878 303 132 433
Cuts at: 3574 3853 4303 4461 4611
Size: 279 450 158 150
Fragments arranged by size:
1329 878 450 433 303 279 256 192
158 150 132 51
BseYI C'CCAG_C
Cuts at: 0 1542 1568 1659 2449 2671 2773 3289 3583
Size: 1542 26 91 790 222 102 516 294
Cuts at: 3583 4129 4611
Size: 546 482
Fragments arranged by size:
1542 790 546 516 482 294 222 102
91 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1277 1291 1420 1996 2053 2070 2641
Size: 925 352 14 129 576 57 17 571
Cuts at: 2641 3930 4611
Size: 1289 681
Fragments arranged by size:
1289 925 681 576 571 352 129 57
17 14
BsiEI CG_ry'CG
Cuts at: 0 304 954 2268 2272 3261 4034 4295 4508
Size: 304 650 1314 4 989 773 261 213
Cuts at: 4508 4611
Size: 103
Fragments arranged by size:
1314 989 773 650 304 261 213 103
4
BsiHKAI G_wGCw'C
Cuts at: 0 512 887 1967 2132 2165 2417 2582 2698
Size: 512 375 1080 165 33 252 165 116
Cuts at: 2698 3457 3497 3594 4045 4526 4611
Size: 759 40 97 451 481 85
Fragments arranged by size:
1080 759 512 481 451 375 252 165
165 116 97 85 40 33
BsmI GAATG_Cn'
Cuts at: 0 2400 2645 4611
Size: 2400 245 1966
Fragments arranged by size:
2400 1966 245
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1172 4611
Size: 1172 3439
Bsp1286I G_dGCh'C
Cuts at: 0 50 274 389 512 887 904 1078 1967
Size: 50 224 115 123 375 17 174 889
Cuts at: 1967 2125 2132 2165 2246 2375 2417 2449 2479
Size: 158 7 33 81 129 42 32 30
Cuts at: 2479 2582 2698 3055 3327 3457 3467 3497 3534
Size: 103 116 357 272 130 10 30 37
Cuts at: 3534 3584 3594 3845 3928 4024 4045 4084 4196
Size: 50 10 251 83 96 21 39 112
Cuts at: 4196 4399 4526 4611
Size: 203 127 85
Fragments arranged by size:
889 375 357 272 251 224 203 174
158 130 129 127 123 116 115 112
103 96 85 83 81 50 50 42
39 37 33 32 30 30 21 17
10 10 7
BspHI T'CATG_A
Cuts at: 0 102 4611
Size: 102 4509
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 327 947 1493 2024 2794 3089 4611
Size: 327 620 546 531 770 295 1522
Fragments arranged by size:
1522 770 620 546 531 327 295
BsrBI CCG'CTC
Cuts at: 0 491 765 1269 2610 3507 3868 4220 4611
Size: 491 274 504 1341 897 361 352 391
Fragments arranged by size:
1341 897 504 491 391 361 352 274
BsrFI r'CCGG_y
Cuts at: 0 72 1093 2063 2180 2267 2382 2883 3260
Size: 72 1021 970 117 87 115 501 377
Cuts at: 3260 3694 4033 4382 4465 4503 4611
Size: 434 339 349 83 38 108
Fragments arranged by size:
1021 970 501 434 377 349 339 117
115 108 87 83 72 38
BssHII G'CGCG_C
Cuts at: 0 1769 2556 4611
Size: 1769 787 2055
Fragments arranged by size:
2055 1769 787
BssSI C'ACGA_G
Cuts at: 0 702 4611
Size: 702 3909
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1300 1539 2964 4611
Size: 1300 239 1425 1647
Fragments arranged by size:
1647 1425 1300 239
BstEII G'GTnAC_C
Cuts at: 0 354 4611
Size: 354 4257
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2152 2773 3526 4611
Size: 2152 621 753 1085
Fragments arranged by size:
2152 1085 753 621
BstYI r'GATC_y
Cuts at: 0 147 1360 1475 1709 1886 3105 4611
Size: 147 1213 115 234 177 1219 1506
Fragments arranged by size:
1506 1219 1213 234 177 147 115
Bsu36I CC'TnA_GG
Cuts at: 0 740 2480 4611
Size: 740 1740 2131
Fragments arranged by size:
2131 1740 740
BtgI C'CryG_G
Cuts at: 0 35 61 590 1112 1776 2488 2766 2930
Size: 35 26 529 522 664 712 278 164
Cuts at: 2930 3213 3234 3386 3968 4152 4196 4460 4565
Size: 283 21 152 582 184 44 264 105
Cuts at: 4565 4611
Size: 46
Fragments arranged by size:
712 664 582 529 522 283 278 264
184 164 152 105 46 44 35 26
21
BtsI GCAGTG_nn'
Cuts at: 0 1253 1602 1905 2541 3168 3594 4210 4611
Size: 1253 349 303 636 627 426 616 401
Fragments arranged by size:
1253 636 627 616 426 401 349 303
DraIII CAC_nnn'GTG
Cuts at: 0 354 2370 4611
Size: 354 2016 2241
Fragments arranged by size:
2241 2016 354
DrdI GACnn_nn'nnGTC
Cuts at: 0 4249 4383 4611
Size: 4249 134 228
Fragments arranged by size:
4249 228 134
EaeI y'GGCC_r
Cuts at: 0 64 301 572 1058 1091 1221 1779 2142
Size: 64 237 271 486 33 130 558 363
Cuts at: 2142 2265 2269 2327 2654 2828 2885 3258 3758
Size: 123 4 58 327 174 57 373 500
Cuts at: 3758 3863 3965 4031 4266 4292 4463 4505 4611
Size: 105 102 66 235 26 171 42 106
Fragments arranged by size:
558 500 486 373 363 327 271 237
235 174 171 130 123 106 105 102
66 64 58 57 42 33 26 4
EagI C'GGCC_G
Cuts at: 0 301 2265 2269 3258 4031 4292 4505 4611
Size: 301 1964 4 989 773 261 213 106
Fragments arranged by size:
1964 989 773 301 261 213 106 4
EarI CTCTTCn'nnn_
Cuts at: 0 671 1127 1873 1939 2524 3445 4013 4282
Size: 671 456 746 66 585 921 568 269
Cuts at: 4282 4611
Size: 329
Fragments arranged by size:
921 746 671 585 568 456 329 269
66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1704 3279 3783 3784 4359 4611
Size: 1704 1575 504 1 575 252
Fragments arranged by size:
1704 1575 575 504 252 1
EcoICRI GAG'CTC
Cuts at: 0 885 1965 2130 2163 2580 3455 4043 4611
Size: 885 1080 165 33 417 875 588 568
Fragments arranged by size:
1080 885 875 588 568 417 165 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 46 496 778 813 916 1774 1867
Size: 34 12 450 282 35 103 858 93
Cuts at: 1867 1897 1933 2068 2095 2299 2543 2889 2894
Size: 30 36 135 27 204 244 346 5
Cuts at: 2894 3006 3165 3433 3469 3856 3975 4156 4611
Size: 112 159 268 36 387 119 181 455
Fragments arranged by size:
858 455 450 387 346 282 268 244
204 181 159 135 119 112 103 93
36 36 35 34 30 27 12 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 334 2230 2356 4611
Size: 334 1896 126 2255
Fragments arranged by size:
2255 1896 334 126
EcoO109I rG'GnC_Cy
Cuts at: 0 1074 1872 2484 2507 2544 2840 2936 3051
Size: 1074 798 612 23 37 296 96 115
Cuts at: 3051 3960 4020 4021 4396 4611
Size: 909 60 1 375 215
Fragments arranged by size:
1074 909 798 612 375 296 215 115
96 60 37 23 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3747 4611
Size: 3747 864
FseI GG_CCGG'CC
Cuts at: 0 1097 2271 4037 4611
Size: 1097 1174 1766 574
Fragments arranged by size:
1766 1174 1097 574
HaeII r_GCGC'y
Cuts at: 0 1029 1239 1435 1675 2033 3212 3321 4611
Size: 1029 210 196 240 358 1179 109 1290
Fragments arranged by size:
1290 1179 1029 358 240 210 196 109
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 655 687 1308 1340 1990 2022 2272 2304
Size: 655 32 621 32 650 32 250 32
Cuts at: 2304 2716 2748 2887 2919 3070 3102 4611
Size: 412 32 139 32 151 32 1509
Fragments arranged by size:
1509 655 650 621 412 250 151 139
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 4254 4611
Size: 4254 357
HindIII A'AGCT_T
Cuts at: 0 198 1456 4611
Size: 198 1258 3155
Fragments arranged by size:
3155 1258 198
KasI G'GCGC_C
Cuts at: 0 1025 1235 1431 2029 4611
Size: 1025 210 196 598 2582
Fragments arranged by size:
2582 1025 598 210 196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1724 3770 4581 4611
Size: 1724 2046 811 30
Fragments arranged by size:
2046 1724 811 30
MscI TGG'CCA
Cuts at: 0 2656 2830 3760 4268 4611
Size: 2656 174 930 508 343
Fragments arranged by size:
2656 930 508 343 174
MslI CAynn'nnrTG
Cuts at: 0 92 545 868 874 2018 4611
Size: 92 453 323 6 1144 2593
Fragments arranged by size:
2593 1144 453 323 92 6
MspA1I CmG'CkG
Cuts at: 0 69 261 756 1404 1422 1731 1752 1778
Size: 69 192 495 648 18 309 21 26
Cuts at: 1778 1794 1824 1953 2490 2573 2837 3780 3970
Size: 16 30 129 537 83 264 943 190
Cuts at: 3970 4050 4116 4154 4185 4319 4537 4611
Size: 80 66 38 31 134 218 74
Fragments arranged by size:
943 648 537 495 309 264 218 192
190 134 129 83 80 74 69 66
38 31 30 26 21 18 16
NaeI GCC'GGC
Cuts at: 0 1095 2065 2182 2269 2384 2885 3262 3696
Size: 1095 970 117 87 115 501 377 434
Cuts at: 3696 4035 4467 4505 4611
Size: 339 432 38 106
Fragments arranged by size:
1095 970 501 434 432 377 339 117
115 106 87 38
NarI GG'CG_CC
Cuts at: 0 1026 1236 1432 2030 4611
Size: 1026 210 196 598 2581
Fragments arranged by size:
2581 1026 598 210 196
NcoI C'CATG_G
Cuts at: 0 590 1112 3386 4565 4611
Size: 590 522 2274 1179 46
Fragments arranged by size:
2274 1179 590 522 46
NgoMIV G'CCGG_C
Cuts at: 0 1093 2063 2180 2267 2382 2883 3260 3694
Size: 1093 970 117 87 115 501 377 434
Cuts at: 3694 4033 4465 4503 4611
Size: 339 432 38 108
Fragments arranged by size:
1093 970 501 434 432 377 339 117
115 108 87 38
NspI r_CATG'y
Cuts at: 0 213 640 4611
Size: 213 427 3971
Fragments arranged by size:
3971 427 213
PasI CC'CwG_GG
Cuts at: 0 2034 2503 3048 4611
Size: 2034 469 545 1563
Fragments arranged by size:
2034 1563 545 469
PflMI CCAn_nnn'nTGG
Cuts at: 0 125 1575 2214 2238 3411 4611
Size: 125 1450 639 24 1173 1200
Fragments arranged by size:
1450 1200 1173 639 125 24
PfoI T'CCnGG_A
Cuts at: 0 143 4302 4611
Size: 143 4159 309
Fragments arranged by size:
4159 309 143
PmlI CAC'GTG
Cuts at: 0 4527 4611
Size: 4527 84
PpuMI rG'GwC_Cy
Cuts at: 0 2484 2936 4611
Size: 2484 452 1675
Fragments arranged by size:
2484 1675 452
PshAI GACnn'nnGTC
Cuts at: 0 27 4611
Size: 27 4584
PspOMI G'GGCC_C
Cuts at: 0 1074 3051 4020 4192 4395 4611
Size: 1074 1977 969 172 203 216
Fragments arranged by size:
1977 1074 969 216 203 172
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1474 1506 4611
Size: 1474 32 3105
Fragments arranged by size:
3105 1474 32
PstI C_TGCA'G
Cuts at: 0 335 1454 1490 1535 1823 1922 1973 2717
Size: 335 1119 36 45 288 99 51 744
Cuts at: 2717 3785 4445 4611
Size: 1068 660 166
Fragments arranged by size:
1119 1068 744 660 335 288 166 99
51 45 36
PvuII CAG'CTG
Cuts at: 0 261 756 1731 1752 1794 1824 4116 4611
Size: 261 495 975 21 42 30 2292 495
Fragments arranged by size:
2292 975 495 495 261 42 30 21
SacI G_AGCT'C
Cuts at: 0 887 1967 2132 2165 2582 3457 4045 4611
Size: 887 1080 165 33 417 875 588 566
Fragments arranged by size:
1080 887 875 588 566 417 165 33
SacII CC_GC'GG
Cuts at: 0 1779 2491 3971 4155 4611
Size: 1779 712 1480 184 456
Fragments arranged by size:
1779 1480 712 456 184
SalI G'TCGA_C
Cuts at: 0 4252 4611
Size: 4252 359
SanDI GG'GwC_CC
Cuts at: 0 2936 4611
Size: 2936 1675
SapI GCTCTTCn'nnn_
Cuts at: 0 1873 1939 2524 3445 4611
Size: 1873 66 585 921 1166
Fragments arranged by size:
1873 1166 921 585 66
SbfI CC_TGCA'GG
Cuts at: 0 1535 4445 4611
Size: 1535 2910 166
Fragments arranged by size:
2910 1535 166
SexAI A'CCwGG_T
Cuts at: 0 425 4611
Size: 425 4186
SfcI C'TryA_G
Cuts at: 0 331 1356 1450 1486 1531 1819 1918 1969
Size: 331 1025 94 36 45 288 99 51
Cuts at: 1969 2713 3781 4441 4480 4611
Size: 744 1068 660 39 131
Fragments arranged by size:
1068 1025 744 660 331 288 131 99
94 51 45 39 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4234 4611
Size: 4234 377
SfoI GGC'GCC
Cuts at: 0 1027 1237 1433 2031 4611
Size: 1027 210 196 598 2580
Fragments arranged by size:
2580 1027 598 210 196
SgrAI Cr'CCGG_yG
Cuts at: 0 3694 4611
Size: 3694 917
SmaI CCC'GGG
Cuts at: 0 3923 4240 4611
Size: 3923 317 371
Fragments arranged by size:
3923 371 317
SmlI C'TyrA_G
Cuts at: 0 151 849 1798 1975 2109 3294 3496 4258
Size: 151 698 949 177 134 1185 202 762
Cuts at: 4258 4611
Size: 353
Fragments arranged by size:
1185 949 762 698 353 202 177 151
134
SphI G_CATG'C
Cuts at: 0 213 640 4611
Size: 213 427 3971
Fragments arranged by size:
3971 427 213
SrfI GCCC'GGGC
Cuts at: 0 3923 4611
Size: 3923 688
StuI AGG'CCT
Cuts at: 0 4517 4611
Size: 4517 94
StyI C'CwwG_G
Cuts at: 0 590 1112 2420 2843 2891 3386 3650 4232
Size: 590 522 1308 423 48 495 264 582
Cuts at: 4232 4565 4611
Size: 333 46
Fragments arranged by size:
1308 590 582 522 495 423 333 264
48 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 373 475 1567 1651 4611
Size: 373 102 1092 84 2960
Fragments arranged by size:
2960 1092 373 102 84
TatI w'GTAC_w
Cuts at: 0 230 1517 4611
Size: 230 1287 3094
Fragments arranged by size:
3094 1287 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2688 2720 3001 3033 4516 4548 4611
Size: 2688 32 281 32 1483 32 63
Fragments arranged by size:
2688 1483 281 63 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1320 4611
Size: 1320 3291
XbaI T'CTAG_A
Cuts at: 0 1461 4611
Size: 1461 3150
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 873 1422 1842 4611
Size: 873 549 420 2769
Fragments arranged by size:
2769 873 549 420
XmaI C'CCGG_G
Cuts at: 0 3921 4238 4611
Size: 3921 317 373
Fragments arranged by size:
3921 373 317
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FseI HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PsrI PstI PvuII SacI SacII SalI SanDI SapI
SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI
BmtI BsiWI BspEI BsrDI BsrGI BstBI BstZ17I BtgZI
ClaI CspCI DraI EcoRI EcoRV FspI FspAI HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PsiI PspXI PvuI RsrII
ScaI SgfI SnaBI SpeI SspI SwaI XhoI XmnI
ZraI