Restriction map for
pFN21AE3471
(Linear) (Six-base) MAPSORT of: KIEE3471.seq Check: 6795 from: 1 to: 4356
KIEE3471.orf
With 182 enzymes: SgfI *
July 3, 2012 17:55 ..
AccI GT'mk_AC
Cuts at: 0 2608 4356
Size: 2608 1748
Acc65I G'GTAC_C
Cuts at: 0 2633 4356
Size: 2633 1723
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 1354 1612 1871 2962 3800 4356
Size: 225 1129 258 259 1091 838 556
Fragments arranged by size:
1129 1091 838 556 259 258 225
AfeI AGC'GCT
Cuts at: 0 780 2956 4356
Size: 780 2176 1400
Fragments arranged by size:
2176 1400 780
AflIII A'CryG_T
Cuts at: 0 1142 1794 3806 4356
Size: 1142 652 2012 550
Fragments arranged by size:
2012 1142 652 550
AleI CACnn'nnGTG
Cuts at: 0 3499 3805 4356
Size: 3499 306 551
Fragments arranged by size:
3499 551 306
AlwNI CAG_nnn'CTG
Cuts at: 0 144 468 1002 1172 3631 4033 4356
Size: 144 324 534 170 2459 402 323
Fragments arranged by size:
2459 534 402 324 323 170 144
ApaI G_GGCC'C
Cuts at: 0 253 326 4356
Size: 253 73 4030
Fragments arranged by size:
4030 253 73
ApoI r'AATT_y
Cuts at: 0 2110 2261 2887 3293 3578 3930 4356
Size: 2110 151 626 406 285 352 426
Fragments arranged by size:
2110 626 426 406 352 285 151
AseI AT'TA_AT
Cuts at: 0 2773 4356
Size: 2773 1583
AvaI C'yCGr_G
Cuts at: 0 246 1074 3604 4356
Size: 246 828 2530 752
Fragments arranged by size:
2530 828 752 246
BamHI G'GATC_C
Cuts at: 0 2943 3838 4356
Size: 2943 895 518
Fragments arranged by size:
2943 895 518
BanI G'GyrC_C
Cuts at: 0 556 1175 2633 3228 4356
Size: 556 619 1458 595 1128
Fragments arranged by size:
1458 1128 619 595 556
BanII G_rGCy'C
Cuts at: 0 253 326 451 1081 4356
Size: 253 73 125 630 3275
Fragments arranged by size:
3275 630 253 125 73
BbeI G_GCGC'C
Cuts at: 0 1179 4356
Size: 1179 3177
BbsI GAAGACnn'nnnn_
Cuts at: 0 351 1924 3084 3266 4356
Size: 351 1573 1160 182 1090
Fragments arranged by size:
1573 1160 1090 351 182
BbvCI CC'TCA_GC
Cuts at: 0 101 946 1439 3992 4356
Size: 101 845 493 2553 364
Fragments arranged by size:
2553 845 493 364 101
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2851 2885 4356
Size: 2851 34 1471
Fragments arranged by size:
2851 1471 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2716 4291 4356
Size: 2716 1575 65
Fragments arranged by size:
2716 1575 65
BfrBI ATG'CAT
Cuts at: 0 4248 4356
Size: 4248 108
BglI GCCn_nnn'nGGC
Cuts at: 0 223 490 713 3349 4356
Size: 223 267 223 2636 1007
Fragments arranged by size:
2636 1007 267 223 223
BglII A'GATC_T
Cuts at: 0 1261 3027 3639 4356
Size: 1261 1766 612 717
Fragments arranged by size:
1766 1261 717 612
BlpI GC'TnA_GC
Cuts at: 0 3398 3740 4356
Size: 3398 342 616
Fragments arranged by size:
3398 616 342
Bme1580I G_kGCm'C
Cuts at: 0 253 326 4356
Size: 253 73 4030
Fragments arranged by size:
4030 253 73
BmgBI CAC'GTC
Cuts at: 0 1143 4356
Size: 1143 3213
BmrI ACTGGGnnnn_n'
Cuts at: 0 447 964 2097 2665 4016 4356
Size: 447 517 1133 568 1351 340
Fragments arranged by size:
1351 1133 568 517 447 340
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 2201 3467 4356
Size: 280 1921 1266 889
Fragments arranged by size:
1921 1266 889 280
Bpu10I CC'TnA_GC
Cuts at: 0 43 101 946 1439 1958 3992 4356
Size: 43 58 845 493 519 2034 364
Fragments arranged by size:
2034 845 519 493 364 58 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1563 4356
Size: 1563 2793
BsaAI yAC'GTr
Cuts at: 0 3807 4356
Size: 3807 549
BsaHI Gr'CG_yC
Cuts at: 0 315 1176 4356
Size: 315 861 3180
Fragments arranged by size:
3180 861 315
BsaWI w'CCGG_w
Cuts at: 0 3498 4356
Size: 3498 858
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 2174 2204 2784 2814 3014 3044
Size: 46 30 2098 30 580 30 200 30
Cuts at: 3044 4356
Size: 1312
Fragments arranged by size:
2098 1312 580 200 46 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 222 537 540 1020 1985 4356
Size: 219 3 315 3 480 965 2371
Fragments arranged by size:
2371 965 480 315 219 3 3
BseYI C'CCAG_C
Cuts at: 0 24 47 371 474 560 1021 1102 4237
Size: 24 23 324 103 86 461 81 3135
Cuts at: 4237 4356
Size: 119
Fragments arranged by size:
3135 461 324 119 103 86 81 24
23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2943 3376 4356
Size: 2943 433 980
Fragments arranged by size:
2943 980 433
BsiEI CG_ry'CG
Cuts at: 0 200 768 2605 4356
Size: 200 568 1837 1751
Fragments arranged by size:
1837 1751 568 200
BsiHKAI G_wGCw'C
Cuts at: 0 451 647 1208 4356
Size: 451 196 561 3148
Fragments arranged by size:
3148 561 451 196
BsmI GAATG_Cn'
Cuts at: 0 1254 4356
Size: 1254 3102
Bsp1286I G_dGCh'C
Cuts at: 0 253 326 451 647 1081 1208 4356
Size: 253 73 125 196 434 127 3148
Fragments arranged by size:
3148 434 253 196 127 125 73
BspHI T'CATG_A
Cuts at: 0 3051 4356
Size: 3051 1305
BsrBI CCG'CTC
Cuts at: 0 126 743 1097 1131 1181 4356
Size: 126 617 354 34 50 3175
Fragments arranged by size:
3175 617 354 126 50 34
BsrDI GCAATG_nn'
Cuts at: 0 3879 4356
Size: 3879 477
BsrFI r'CCGG_y
Cuts at: 0 647 3569 4356
Size: 647 2922 787
Fragments arranged by size:
2922 787 647
BssHII G'CGCG_C
Cuts at: 0 291 4356
Size: 291 4065
BssSI C'ACGA_G
Cuts at: 0 1376 4356
Size: 1376 2980
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 468 4356
Size: 144 324 3888
Fragments arranged by size:
3888 324 144
BstBI TT'CG_AA
Cuts at: 0 3277 4356
Size: 3277 1079
BstEII G'GTnAC_C
Cuts at: 0 4346 4356
Size: 4346 10
BstXI CCAn_nnnn'nTGG
Cuts at: 0 4282 4356
Size: 4282 74
BstYI r'GATC_y
Cuts at: 0 1261 1288 2943 3027 3249 3542 3639 3838
Size: 1261 27 1655 84 222 293 97 199
Cuts at: 3838 4356
Size: 518
Fragments arranged by size:
1655 1261 518 293 222 199 97 84
27
BstZ17I GTA'TAC
Cuts at: 0 2609 4356
Size: 2609 1747
Bsu36I CC'TnA_GG
Cuts at: 0 920 1003 4356
Size: 920 83 3353
Fragments arranged by size:
3353 920 83
BtgI C'CryG_G
Cuts at: 0 221 4110 4151 4356
Size: 221 3889 41 205
Fragments arranged by size:
3889 221 205 41
BtsI GCAGTG_nn'
Cuts at: 0 621 2813 2960 4146 4356
Size: 621 2192 147 1186 210
Fragments arranged by size:
2192 1186 621 210 147
DraI TTT'AAA
Cuts at: 0 1595 4356
Size: 1595 2761
DraIII CAC_nnn'GTG
Cuts at: 0 3235 4356
Size: 3235 1121
DrdI GACnn_nn'nnGTC
Cuts at: 0 852 4356
Size: 852 3504
EaeI y'GGCC_r
Cuts at: 0 197 618 765 785 2602 4356
Size: 197 421 147 20 1817 1754
Fragments arranged by size:
1817 1754 421 197 147 20
EagI C'GGCC_G
Cuts at: 0 197 765 2602 4356
Size: 197 568 1837 1754
Fragments arranged by size:
1837 1754 568 197
EarI CTCTTCn'nnn_
Cuts at: 0 181 1330 1631 2725 2981 3425 3849 3858
Size: 181 1149 301 1094 256 444 424 9
Cuts at: 3858 4356
Size: 498
Fragments arranged by size:
1149 1094 498 444 424 301 256 181
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 67 358 4356
Size: 67 291 3998
Fragments arranged by size:
3998 291 67
EcoICRI GAG'CTC
Cuts at: 0 449 4356
Size: 449 3907
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 280 1354 1612 1871 2201 2962 3467
Size: 225 55 1074 258 259 330 761 505
Cuts at: 3467 3800 4356
Size: 333 556
Fragments arranged by size:
1074 761 556 505 333 330 259 258
225 55
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4274 4356
Size: 4274 82
EcoO109I rG'GnC_Cy
Cuts at: 0 39 250 323 590 989 4279 4356
Size: 39 211 73 267 399 3290 77
Fragments arranged by size:
3290 399 267 211 77 73 39
EcoRI G'AATT_C
Cuts at: 0 3293 3578 4356
Size: 3293 285 778
Fragments arranged by size:
3293 778 285
EcoRV GAT'ATC
Cuts at: 0 2118 4356
Size: 2118 2238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2247 2511 3849 4356
Size: 2247 264 1338 507
Fragments arranged by size:
2247 1338 507 264
HaeII r_GCGC'y
Cuts at: 0 25 636 782 1179 2958 4356
Size: 25 611 146 397 1779 1398
Fragments arranged by size:
1779 1398 611 397 146 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 808 840 1690 1722 2784 2816
Size: 433 32 343 32 850 32 1062 32
Cuts at: 2816 3771 3803 3928 3960 4155 4187 4356
Size: 955 32 125 32 195 32 169
Fragments arranged by size:
1062 955 850 433 343 195 169 125
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 3380 4356
Size: 3380 976
HindIII A'AGCT_T
Cuts at: 0 1463 4356
Size: 1463 2893
KasI G'GCGC_C
Cuts at: 0 1175 4356
Size: 1175 3181
KpnI G_GTAC'C
Cuts at: 0 2637 4356
Size: 2637 1719
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 560 1671 4356
Size: 44 516 1111 2685
Fragments arranged by size:
2685 1111 516 44
MslI CAynn'nnrTG
Cuts at: 0 2426 3499 3805 3980 4356
Size: 2426 1073 306 175 376
Fragments arranged by size:
2426 1073 376 306 175
MspA1I CmG'CkG
Cuts at: 0 114 223 426 530 564 713 761 1172
Size: 114 109 203 104 34 149 48 411
Cuts at: 1172 1351 1950 2908 3201 3732 4356
Size: 179 599 958 293 531 624
Fragments arranged by size:
958 624 599 531 411 293 203 179
149 114 109 104 48 34
NarI GG'CG_CC
Cuts at: 0 1176 4356
Size: 1176 3180
NcoI C'CATG_G
Cuts at: 0 4110 4356
Size: 4110 246
NotI GC'GGCC_GC
Cuts at: 0 765 4356
Size: 765 3591
NsiI A_TGCA'T
Cuts at: 0 4250 4356
Size: 4250 106
NspI r_CATG'y
Cuts at: 0 1567 1798 2934 3670 4356
Size: 1567 231 1136 736 686
Fragments arranged by size:
1567 1136 736 686 231
PasI CC'CwG_GG
Cuts at: 0 3177 4356
Size: 3177 1179
PciI A'CATG_T
Cuts at: 0 1794 4356
Size: 1794 2562
PpuMI rG'GwC_Cy
Cuts at: 0 4279 4356
Size: 4279 77
PspOMI G'GGCC_C
Cuts at: 0 249 322 4356
Size: 249 73 4034
Fragments arranged by size:
4034 249 73
PstI C_TGCA'G
Cuts at: 0 134 1422 2808 2907 4356
Size: 134 1288 1386 99 1449
Fragments arranged by size:
1449 1386 1288 134 99
PvuII CAG'CTG
Cuts at: 0 1172 1351 2908 3201 3732 4356
Size: 1172 179 1557 293 531 624
Fragments arranged by size:
1557 1172 624 531 293 179
SacI G_AGCT'C
Cuts at: 0 451 4356
Size: 451 3905
SacII CC_GC'GG
Cuts at: 0 224 4356
Size: 224 4132
SapI GCTCTTCn'nnn_
Cuts at: 0 181 2981 4356
Size: 181 2800 1375
Fragments arranged by size:
2800 1375 181
ScaI AGT'ACT
Cuts at: 0 3963 4167 4356
Size: 3963 204 189
Fragments arranged by size:
3963 204 189
SexAI A'CCwGG_T
Cuts at: 0 3231 4356
Size: 3231 1125
SfcI C'TryA_G
Cuts at: 0 130 927 1083 1107 1158 1194 1418 2804
Size: 130 797 156 24 51 36 224 1386
Cuts at: 2804 2903 3957 4356
Size: 99 1054 399
Fragments arranged by size:
1386 1054 797 399 224 156 130 99
51 36 24
SfoI GGC'GCC
Cuts at: 0 1177 4356
Size: 1177 3179
SgrAI Cr'CCGG_yG
Cuts at: 0 647 4356
Size: 647 3709
SmaI CCC'GGG
Cuts at: 0 248 4356
Size: 248 4108
SmlI C'TyrA_G
Cuts at: 0 1578 4356
Size: 1578 2778
SpeI A'CTAG_T
Cuts at: 0 1504 4356
Size: 1504 2852
SrfI GCCC'GGGC
Cuts at: 0 248 4356
Size: 248 4108
SspI AAT'ATT
Cuts at: 0 1884 4356
Size: 1884 2472
StuI AGG'CCT
Cuts at: 0 682 895 925 2037 4356
Size: 682 213 30 1112 2319
Fragments arranged by size:
2319 1112 682 213 30
StyI C'CwwG_G
Cuts at: 0 857 890 1451 1487 1778 2709 2939 4110
Size: 857 33 561 36 291 931 230 1171
Cuts at: 4110 4275 4356
Size: 165 81
Fragments arranged by size:
1171 931 857 561 291 230 165 81
36 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 574 928 4356
Size: 574 354 3428
Fragments arranged by size:
3428 574 354
TatI w'GTAC_w
Cuts at: 0 3961 4165 4356
Size: 3961 204 191
Fragments arranged by size:
3961 204 191
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3014 3046 4356
Size: 3014 32 1310
Fragments arranged by size:
3014 1310 32
Tth111I GACn'n_nGTC
Cuts at: 0 849 4356
Size: 849 3507
XbaI T'CTAG_A
Cuts at: 0 1285 3642 4356
Size: 1285 2357 714
Fragments arranged by size:
2357 1285 714
XmaI C'CCGG_G
Cuts at: 0 246 4356
Size: 246 4110
XmnI GAAnn'nnTTC
Cuts at: 0 1884 2231 3891 4356
Size: 1884 347 1660 465
Fragments arranged by size:
1884 1660 465 347
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AleI AlwNI ApaI
ApoI AseI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MslI MspA1I NarI NcoI
NotI NsiI NspI PasI PciI PpuMI PspOMI PstI
PvuII SacI SacII SapI ScaI SexAI SfcI SfoI
SgrAI SmaI SmlI SpeI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AhdI AlfI AloI
ApaLI AscI AsiSI AvrII BaeI BclI BmtI BplI
BsaI BsaBI BsiWI BsmBI BspEI BspMI BsrGI BtgZI
ClaI CspCI FseI FspI FspAI HpaI MfeI MluI
MscI NaeI NdeI NgoMIV NheI NruI PacI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SalI SanDI SbfI SfiI SgfI SnaBI
SphI SwaI XcmI XhoI ZraI