Restriction map for
pFN21AE3460
(Linear) (Six-base) MAPSORT of: KIEE3460.seq Check: 5868 from: 1 to: 4062
KIEE3460.orf
With 182 enzymes: SgfI *
March 22, 2012 16:34 ..
AccI GT'mk_AC
Cuts at: 0 4 4062
Size: 4 4058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 749 1205 2263 3211 3587 4062
Size: 239 510 456 1058 948 376 475
Fragments arranged by size:
1058 948 510 475 456 376 239
AflII C'TTAA_G
Cuts at: 0 135 2950 4062
Size: 135 2815 1112
Fragments arranged by size:
2815 1112 135
AhdI GACnn_n'nnGTC
Cuts at: 0 783 4062
Size: 783 3279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3287 4062
Size: 3287 775
ApaLI G'TGCA_C
Cuts at: 0 3209 4062
Size: 3209 853
ApoI r'AATT_y
Cuts at: 0 75 325 882 1593 1769 1779 2464 2619
Size: 75 250 557 711 176 10 685 155
Cuts at: 2619 3139 3446 3572 4062
Size: 520 307 126 490
Fragments arranged by size:
711 685 557 520 490 307 250 176
155 126 75 10
AseI AT'TA_AT
Cuts at: 0 1484 4062
Size: 1484 2578
AvaI C'yCGr_G
Cuts at: 0 2177 4062
Size: 2177 1885
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 686 719 4062
Size: 686 33 3343
Fragments arranged by size:
3343 686 33
BamHI G'GATC_C
Cuts at: 0 57 4062
Size: 57 4005
BbsI GAAGACnn'nnnn_
Cuts at: 0 1577 2377 4062
Size: 1577 800 1685
Fragments arranged by size:
1685 1577 800
BfrBI ATG'CAT
Cuts at: 0 424 4062
Size: 424 3638
BglII A'GATC_T
Cuts at: 0 343 477 4062
Size: 343 134 3585
Fragments arranged by size:
3585 343 134
BlpI GC'TnA_GC
Cuts at: 0 2477 2543 4062
Size: 2477 66 1519
Fragments arranged by size:
2477 1519 66
Bme1580I G_kGCm'C
Cuts at: 0 525 3213 4062
Size: 525 2688 849
Fragments arranged by size:
2688 849 525
BmrI ACTGGGnnnn_n'
Cuts at: 0 17 1617 4062
Size: 17 1600 2445
Fragments arranged by size:
2445 1600 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2347 2944 4062
Size: 2347 597 1118
Fragments arranged by size:
2347 1118 597
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1949 2360 4062
Size: 1949 411 1702
Fragments arranged by size:
1949 1702 411
BsaI GGTCTCn'nnnn_
Cuts at: 0 1868 4062
Size: 1868 2194
BsaBI GATnn'nnATC
Cuts at: 0 440 1215 2801 4062
Size: 440 775 1586 1261
Fragments arranged by size:
1586 1261 775 440
BsaWI w'CCGG_w
Cuts at: 0 4025 4062
Size: 4025 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 467 497 1339 1369 1450 1480 4062
Size: 467 30 842 30 81 30 2582
Fragments arranged by size:
2582 842 467 81 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1921 2947 2950 4062
Size: 1921 1026 3 1112
Fragments arranged by size:
1921 1112 1026 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1377 4062
Size: 1377 2685
BsiHKAI G_wGCw'C
Cuts at: 0 3213 4062
Size: 3213 849
BsmI GAATG_Cn'
Cuts at: 0 424 4062
Size: 424 3638
Bsp1286I G_dGCh'C
Cuts at: 0 525 3213 4062
Size: 525 2688 849
Fragments arranged by size:
2688 849 525
BspEI T'CCGG_A
Cuts at: 0 4025 4062
Size: 4025 37
BspHI T'CATG_A
Cuts at: 0 1854 4062
Size: 1854 2208
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 56 4062
Size: 56 4006
BsrDI GCAATG_nn'
Cuts at: 0 2141 4062
Size: 2141 1921
BstAPI GCAn_nnn'nTGC
Cuts at: 0 559 4062
Size: 559 3503
BstEII G'GTnAC_C
Cuts at: 0 3340 4062
Size: 3340 722
BstYI r'GATC_y
Cuts at: 0 57 343 477 2676 4003 4062
Size: 57 286 134 2199 1327 59
Fragments arranged by size:
2199 1327 286 134 59 57
BtgI C'CryG_G
Cuts at: 0 572 4062
Size: 572 3490
BtsI GCAGTG_nn'
Cuts at: 0 4007 4062
Size: 4007 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2111 2146 4062
Size: 2111 35 1916
Fragments arranged by size:
2111 1916 35
DraI TTT'AAA
Cuts at: 0 2018 2399 2624 3045 4062
Size: 2018 381 225 421 1017
Fragments arranged by size:
2018 1017 421 381 225
EaeI y'GGCC_r
Cuts at: 0 3239 4062
Size: 3239 823
EarI CTCTTCn'nnn_
Cuts at: 0 762 1012 1400 1516 1690 1836 2111 2862
Size: 762 250 388 116 174 146 275 751
Cuts at: 2862 2873 4062
Size: 11 1189
Fragments arranged by size:
1189 762 751 388 275 250 174 146
116 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 749 1205 2263 2347 2944 3211 3587
Size: 239 510 456 1058 84 597 267 376
Cuts at: 3587 4062
Size: 475
Fragments arranged by size:
1058 597 510 475 456 376 267 239
84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1181 4062
Size: 1181 2881
EcoRI G'AATT_C
Cuts at: 0 75 1593 1779 4062
Size: 75 1518 186 2283
Fragments arranged by size:
2283 1518 186 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 565 1990 2551 2677 2976 3388 4062
Size: 565 1425 561 126 299 412 674
Fragments arranged by size:
1425 674 565 561 412 299 126
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 769 801 1603 1635 1683 1715
Size: 49 32 688 32 802 32 48 32
Cuts at: 1715 2110 2142 2320 2352 2931 2963 3380 3412
Size: 395 32 178 32 579 32 417 32
Cuts at: 3412 3853 3885 4062
Size: 441 32 177
Fragments arranged by size:
802 688 579 441 417 395 178 177
49 48 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 5 3009 4062
Size: 5 3004 1053
Fragments arranged by size:
3004 1053 5
HindIII A'AGCT_T
Cuts at: 0 313 1154 1266 4062
Size: 313 841 112 2796
Fragments arranged by size:
2796 841 313 112
HpaI GTT'AAC
Cuts at: 0 3009 4062
Size: 3009 1053
MfeI C'AATT_G
Cuts at: 0 271 3193 3265 3720 4062
Size: 271 2922 72 455 342
Fragments arranged by size:
2922 455 342 271 72
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 684 831 1547 2654 4062
Size: 684 147 716 1107 1408
Fragments arranged by size:
1408 1107 716 684 147
MscI TGG'CCA
Cuts at: 0 3241 4062
Size: 3241 821
MslI CAynn'nnrTG
Cuts at: 0 454 590 1708 3218 4062
Size: 454 136 1118 1510 844
Fragments arranged by size:
1510 1118 844 454 136
MspA1I CmG'CkG
Cuts at: 0 700 1314 1681 1815 4062
Size: 700 614 367 134 2247
Fragments arranged by size:
2247 700 614 367 134
NcoI C'CATG_G
Cuts at: 0 572 4062
Size: 572 3490
NsiI A_TGCA'T
Cuts at: 0 426 4062
Size: 426 3636
NspI r_CATG'y
Cuts at: 0 468 612 1974 2841 3186 3754 3899 4062
Size: 468 144 1362 867 345 568 145 163
Fragments arranged by size:
1362 867 568 468 345 163 145 144
PflMI CCAn_nnn'nTGG
Cuts at: 0 573 3964 4062
Size: 573 3391 98
Fragments arranged by size:
3391 573 98
PshAI GACnn'nnGTC
Cuts at: 0 1850 4062
Size: 1850 2212
PsiI TTA'TAA
Cuts at: 0 2567 4062
Size: 2567 1495
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3981 4013 4062
Size: 3981 32 49
Fragments arranged by size:
3981 49 32
PstI C_TGCA'G
Cuts at: 0 540 4062
Size: 540 3522
PvuII CAG'CTG
Cuts at: 0 1314 1681 1815 4062
Size: 1314 367 134 2247
Fragments arranged by size:
2247 1314 367 134
SalI G'TCGA_C
Cuts at: 0 3 4062
Size: 3 4059
SapI GCTCTTCn'nnn_
Cuts at: 0 2873 4062
Size: 2873 1189
SfcI C'TryA_G
Cuts at: 0 536 1052 1436 1471 4062
Size: 536 516 384 35 2591
Fragments arranged by size:
2591 536 516 384 35
SmlI C'TyrA_G
Cuts at: 0 135 1964 2177 2375 2950 4062
Size: 135 1829 213 198 575 1112
Fragments arranged by size:
1829 1112 575 213 198 135
SpeI A'CTAG_T
Cuts at: 0 1093 4057 4062
Size: 1093 2964 5
Fragments arranged by size:
2964 1093 5
SphI G_CATG'C
Cuts at: 0 2841 4062
Size: 2841 1221
SspI AAT'ATT
Cuts at: 0 153 2551 2861 3584 4062
Size: 153 2398 310 723 478
Fragments arranged by size:
2398 723 478 310 153
StuI AGG'CCT
Cuts at: 0 2720 4062
Size: 2720 1342
StyI C'CwwG_G
Cuts at: 0 400 572 744 3540 4062
Size: 400 172 172 2796 522
Fragments arranged by size:
2796 522 400 172 172
TatI w'GTAC_w
Cuts at: 0 3642 4062
Size: 3642 420
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1339 1371 4062
Size: 1339 32 2691
Fragments arranged by size:
2691 1339 32
XbaI T'CTAG_A
Cuts at: 0 1428 4062
Size: 1428 2634
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 751 3492 4062
Size: 751 2741 570
Fragments arranged by size:
2741 751 570
XhoI C'TCGA_G
Cuts at: 0 2177 4062
Size: 2177 1885
XmnI GAAnn'nnTTC
Cuts at: 0 545 3572 3993 4062
Size: 545 3027 421 69
Fragments arranged by size:
3027 545 421 69
Enzymes that do cut:
AccI AcuI AflII AhdI AlfI ApaLI ApoI AseI
AvaI BaeI BamHI BbsI BfrBI BglII BlpI Bme1580I
BmrI BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI
BstAPI BstEII BstYI BtgI BtsI CspCI DraI EaeI
EarI Eco57MI EcoNI EcoRI FalI Hin4I HincII HindIII
HpaI MfeI MmeI MscI MslI MspA1I NcoI NsiI
NspI PflMI PshAI PsiI PsrI PstI PvuII SalI
SapI SfcI SmlI SpeI SphI SspI StuI StyI
TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AleI
AloI AlwNI ApaI AscI AsiSI AvrII BanI BanII
BbeI BbvCI BcgI BciVI BclI BglI BmgBI BmtI
BplI Bpu10I BsaAI BsaHI BseYI BsiEI BsiWI BsmBI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI DraIII DrdI EagI EciI EcoICRI
EcoO109I EcoRV FseI FspI FspAI HaeII KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PvuI RsrII SacI SacII SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SwaI TaqII Tth111I XmaI ZraI