Restriction map for
pFN21AE3459
(Linear) (Six-base) MAPSORT of: KIEE3459.seq Check: 1762 from: 1 to: 4026
KIEE3459.orf
With 182 enzymes: SgfI *
February 23, 2012 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 12 4026
Size: 12 4014
AccI GT'mk_AC
Cuts at: 0 329 791 2819 4026
Size: 329 462 2028 1207
Fragments arranged by size:
2028 1207 462 329
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1209 1357 2774 3518 3584 4026
Size: 1209 148 1417 744 66 442
Fragments arranged by size:
1417 1209 744 442 148 66
AleI CACnn'nnGTG
Cuts at: 0 2672 4026
Size: 2672 1354
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1183 1215 4026
Size: 1183 32 2811
Fragments arranged by size:
2811 1183 32
AlwNI CAG_nnn'CTG
Cuts at: 0 27 144 545 911 1006 1475 1540 2430
Size: 27 117 401 366 95 469 65 890
Cuts at: 2430 3214 3310 3853 3961 4026
Size: 784 96 543 108 65
Fragments arranged by size:
890 784 543 469 401 366 117 108
96 95 65 65 27
ApaI G_GGCC'C
Cuts at: 0 662 1534 1748 1795 4026
Size: 662 872 214 47 2231
Fragments arranged by size:
2231 872 662 214 47
ApaLI G'TGCA_C
Cuts at: 0 2167 4026
Size: 2167 1859
ApoI r'AATT_y
Cuts at: 0 277 1624 4026
Size: 277 1347 2402
Fragments arranged by size:
2402 1347 277
AvaI C'yCGr_G
Cuts at: 0 50 1032 1619 1701 2148 2906 3445 3515
Size: 50 982 587 82 447 758 539 70
Cuts at: 3515 4026
Size: 511
Fragments arranged by size:
982 758 587 539 511 447 82 70
50
AvrII C'CTAG_G
Cuts at: 0 1779 3634 4026
Size: 1779 1855 392
Fragments arranged by size:
1855 1779 392
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2905 2938 4026
Size: 2905 33 1088
Fragments arranged by size:
2905 1088 33
BamHI G'GATC_C
Cuts at: 0 2183 4026
Size: 2183 1843
BanI G'GyrC_C
Cuts at: 0 1783 2003 3230 3523 4026
Size: 1783 220 1227 293 503
Fragments arranged by size:
1783 1227 503 293 220
BanII G_rGCy'C
Cuts at: 0 58 662 950 1534 1708 1748 1795 2732
Size: 58 604 288 584 174 40 47 937
Cuts at: 2732 2975 3085 3323 3370 4026
Size: 243 110 238 47 656
Fragments arranged by size:
937 656 604 584 288 243 238 174
110 58 47 47 40
BbeI G_GCGC'C
Cuts at: 0 2007 4026
Size: 2007 2019
BbsI GAAGACnn'nnnn_
Cuts at: 0 605 1149 3779 4026
Size: 605 544 2630 247
Fragments arranged by size:
2630 605 544 247
BbvCI CC'TCA_GC
Cuts at: 0 3091 4026
Size: 3091 935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 220 254 4026
Size: 220 34 3772
Fragments arranged by size:
3772 220 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2656 3151 4026
Size: 2656 495 875
Fragments arranged by size:
2656 875 495
BclI T'GATC_A
Cuts at: 0 1091 1292 4026
Size: 1091 201 2734
Fragments arranged by size:
2734 1091 201
BglI GCCn_nnn'nGGC
Cuts at: 0 1636 3838 4026
Size: 1636 2202 188
Fragments arranged by size:
2202 1636 188
BglII A'GATC_T
Cuts at: 0 2801 4026
Size: 2801 1225
BlpI GC'TnA_GC
Cuts at: 0 3317 4026
Size: 3317 709
Bme1580I G_kGCm'C
Cuts at: 0 662 1534 1684 1731 1748 1795 2171 2415
Size: 662 872 150 47 17 47 376 244
Cuts at: 2415 3235 4026
Size: 820 791
Fragments arranged by size:
872 820 791 662 376 244 150 47
47 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 2069 2425 2448 2528 3088 4026
Size: 2069 356 23 80 560 938
Fragments arranged by size:
2069 938 560 356 80 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 507 2703 3034 3307 3749 4026
Size: 363 144 2196 331 273 442 277
Fragments arranged by size:
2196 442 363 331 277 273 144
Bpu10I CC'TnA_GC
Cuts at: 0 705 2125 3091 4026
Size: 705 1420 966 935
Fragments arranged by size:
1420 966 935 705
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 934 2494 2509 2659 3670 4026
Size: 208 726 1560 15 150 1011 356
Fragments arranged by size:
1560 1011 726 356 208 150 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 1103 2903 3547 4026
Size: 1103 1800 644 479
Fragments arranged by size:
1800 1103 644 479
BsaAI yAC'GTr
Cuts at: 0 3124 3940 4026
Size: 3124 816 86
Fragments arranged by size:
3124 816 86
BsaHI Gr'CG_yC
Cuts at: 0 2004 4026
Size: 2004 2022
BsaWI w'CCGG_w
Cuts at: 0 1160 3602 4026
Size: 1160 2442 424
Fragments arranged by size:
2442 1160 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 361 391 749 779 842 872 2676 2706
Size: 361 30 358 30 63 30 1804 30
Cuts at: 2706 3319 3349 4026
Size: 613 30 677
Fragments arranged by size:
1804 677 613 361 358 63 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 623 863 1503 1589 3067 3501 3598 3621
Size: 623 240 640 86 1478 434 97 23
Cuts at: 3621 3667 4026
Size: 46 359
Fragments arranged by size:
1478 640 623 434 359 240 97 86
46 23
BseYI C'CCAG_C
Cuts at: 0 27 581 1351 1526 1974 2322 3401 3657
Size: 27 554 770 175 448 348 1079 256
Cuts at: 3657 3846 4026
Size: 189 180
Fragments arranged by size:
1079 770 554 448 348 256 189 180
175 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1044 1833 2790 4026
Size: 1044 789 957 1236
Fragments arranged by size:
1236 1044 957 789
BsiEI CG_ry'CG
Cuts at: 0 1465 4026
Size: 1465 2561
BsiHKAI G_wGCw'C
Cuts at: 0 200 633 1513 1722 2171 3323 4026
Size: 200 433 880 209 449 1152 703
Fragments arranged by size:
1152 880 703 449 433 209 200
BsmI GAATG_Cn'
Cuts at: 0 1648 1669 4026
Size: 1648 21 2357
Fragments arranged by size:
2357 1648 21
Bsp1286I G_dGCh'C
Cuts at: 0 58 200 633 662 950 1513 1534 1684
Size: 58 142 433 29 288 563 21 150
Cuts at: 1684 1708 1722 1731 1748 1795 2171 2415 2732
Size: 24 14 9 17 47 376 244 317
Cuts at: 2732 2975 3085 3235 3323 3370 4026
Size: 243 110 150 88 47 656
Fragments arranged by size:
656 563 433 376 317 288 244 243
150 150 142 110 88 58 47 47
29 24 21 17 14 9
BspEI T'CCGG_A
Cuts at: 0 1160 4026
Size: 1160 2866
BspHI T'CATG_A
Cuts at: 0 558 1295 1967 4026
Size: 558 737 672 2059
Fragments arranged by size:
2059 737 672 558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 2579 2774 4026
Size: 12 2567 195 1252
Fragments arranged by size:
2567 1252 195 12
BsrBI CCG'CTC
Cuts at: 0 2784 4026
Size: 2784 1242
BsrDI GCAATG_nn'
Cuts at: 0 292 1701 4026
Size: 292 1409 2325
Fragments arranged by size:
2325 1409 292
BsrFI r'CCGG_y
Cuts at: 0 115 737 1096 4026
Size: 115 622 359 2930
Fragments arranged by size:
2930 622 359 115
BsrGI T'GTAC_A
Cuts at: 0 146 4026
Size: 146 3880
BssSI C'ACGA_G
Cuts at: 0 262 4026
Size: 262 3764
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1475 4026
Size: 1475 2551
BstEII G'GTnAC_C
Cuts at: 0 1183 4026
Size: 1183 2843
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1071 2627 3078 4026
Size: 1071 1556 451 948
Fragments arranged by size:
1556 1071 948 451
BstYI r'GATC_y
Cuts at: 0 1067 1124 2183 2801 4026
Size: 1067 57 1059 618 1225
Fragments arranged by size:
1225 1067 1059 618 57
Bsu36I CC'TnA_GG
Cuts at: 0 713 2189 4026
Size: 713 1476 1837
Fragments arranged by size:
1837 1476 713
BtgI C'CryG_G
Cuts at: 0 1673 1770 2917 2976 3262 3370 3833 4026
Size: 1673 97 1147 59 286 108 463 193
Fragments arranged by size:
1673 1147 463 286 193 108 97 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 134 591 4026
Size: 134 457 3435
Fragments arranged by size:
3435 457 134
BtsI GCAGTG_nn'
Cuts at: 0 86 238 648 1593 1682 3228 3749 4026
Size: 86 152 410 945 89 1546 521 277
Fragments arranged by size:
1546 945 521 410 277 152 89 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 735 770 4026
Size: 735 35 3256
Fragments arranged by size:
3256 735 35
DraI TTT'AAA
Cuts at: 0 2874 4026
Size: 2874 1152
DraIII CAC_nnn'GTG
Cuts at: 0 1755 4026
Size: 1755 2271
EaeI y'GGCC_r
Cuts at: 0 266 1204 1462 2099 2282 3118 3373 4026
Size: 266 938 258 637 183 836 255 653
Fragments arranged by size:
938 836 653 637 266 258 255 183
EagI C'GGCC_G
Cuts at: 0 1462 4026
Size: 1462 2564
EarI CTCTTCn'nnn_
Cuts at: 0 1430 3452 3863 4026
Size: 1430 2022 411 163
Fragments arranged by size:
2022 1430 411 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3075 4026
Size: 3075 951
EcoICRI GAG'CTC
Cuts at: 0 3321 4026
Size: 3321 705
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 363 507 1209 1357 2703 2774 3034 3307
Size: 363 144 702 148 1346 71 260 273
Cuts at: 3307 3518 3584 3749 4026
Size: 211 66 165 277
Fragments arranged by size:
1346 702 363 277 273 260 211 165
148 144 71 66
EcoNI CCTnn'n_nnAGG
Cuts at: 0 903 4026
Size: 903 3123
EcoO109I rG'GnC_Cy
Cuts at: 0 320 586 709 908 1426 1530 1537 1745
Size: 320 266 123 199 518 104 7 208
Cuts at: 1745 1791 3839 3889 3903 4026
Size: 46 2048 50 14 123
Fragments arranged by size:
2048 518 320 266 208 199 123 123
104 50 46 14 7
EcoRI G'AATT_C
Cuts at: 0 277 4026
Size: 277 3749
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3544 4026
Size: 3544 482
HaeII r_GCGC'y
Cuts at: 0 2007 3392 3408 4026
Size: 2007 1385 16 618
Fragments arranged by size:
2007 1385 618 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 880 912 1433 1465 1565 1597
Size: 412 32 436 32 521 32 100 32
Cuts at: 1597 2168 2182 2200 2214 3319 3351 3667 3699
Size: 571 14 18 14 1105 32 316 32
Cuts at: 3699 4026
Size: 327
Fragments arranged by size:
1105 571 521 436 412 327 316 100
32 32 32 32 32 32 18 14
14
HincII GTy'rAC
Cuts at: 0 3468 4026
Size: 3468 558
HindIII A'AGCT_T
Cuts at: 0 460 2131 4026
Size: 460 1671 1895
Fragments arranged by size:
1895 1671 460
KasI G'GCGC_C
Cuts at: 0 2003 4026
Size: 2003 2023
MfeI C'AATT_G
Cuts at: 0 479 2810 4026
Size: 479 2331 1216
Fragments arranged by size:
2331 1216 479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2821 3084 4026
Size: 2821 263 942
Fragments arranged by size:
2821 942 263
MscI TGG'CCA
Cuts at: 0 268 2101 2284 4026
Size: 268 1833 183 1742
Fragments arranged by size:
1833 1742 268 183
MslI CAynn'nnrTG
Cuts at: 0 746 2672 2827 2899 3166 3267 3676 4026
Size: 746 1926 155 72 267 101 409 350
Fragments arranged by size:
1926 746 409 350 267 155 101 72
MspA1I CmG'CkG
Cuts at: 0 813 866 1763 2427 2595 4026
Size: 813 53 897 664 168 1431
Fragments arranged by size:
1431 897 813 664 168 53
NaeI GCC'GGC
Cuts at: 0 739 4026
Size: 739 3287
NarI GG'CG_CC
Cuts at: 0 2004 4026
Size: 2004 2022
NcoI C'CATG_G
Cuts at: 0 1673 1770 2976 3833 4026
Size: 1673 97 1206 857 193
Fragments arranged by size:
1673 1206 857 193 97
NdeI CA'TA_TG
Cuts at: 0 2276 4026
Size: 2276 1750
NgoMIV G'CCGG_C
Cuts at: 0 737 4026
Size: 737 3289
NspI r_CATG'y
Cuts at: 0 1218 1694 3165 3301 4026
Size: 1218 476 1471 136 725
Fragments arranged by size:
1471 1218 725 476 136
PasI CC'CwG_GG
Cuts at: 0 1854 2335 2962 4026
Size: 1854 481 627 1064
Fragments arranged by size:
1854 1064 627 481
PflMI CCAn_nnn'nTGG
Cuts at: 0 1026 1540 1674 3154 4026
Size: 1026 514 134 1480 872
Fragments arranged by size:
1480 1026 872 514 134
PfoI T'CCnGG_A
Cuts at: 0 588 3323 4026
Size: 588 2735 703
Fragments arranged by size:
2735 703 588
PmlI CAC'GTG
Cuts at: 0 3124 4026
Size: 3124 902
PpuMI rG'GwC_Cy
Cuts at: 0 320 586 4026
Size: 320 266 3440
Fragments arranged by size:
3440 320 266
PshAI GACnn'nnGTC
Cuts at: 0 325 4026
Size: 325 3701
PspOMI G'GGCC_C
Cuts at: 0 658 1530 1744 1791 4026
Size: 658 872 214 47 2235
Fragments arranged by size:
2235 872 658 214 47
PspXI vC'TCGA_Gb
Cuts at: 0 1032 4026
Size: 1032 2994
PstI C_TGCA'G
Cuts at: 0 23 233 2351 3210 3756 4026
Size: 23 210 2118 859 546 270
Fragments arranged by size:
2118 859 546 270 210 23
PvuII CAG'CTG
Cuts at: 0 813 866 1763 2427 2595 4026
Size: 813 53 897 664 168 1431
Fragments arranged by size:
1431 897 813 664 168 53
SacI G_AGCT'C
Cuts at: 0 3323 4026
Size: 3323 703
SanDI GG'GwC_CC
Cuts at: 0 320 4026
Size: 320 3706
SapI GCTCTTCn'nnn_
Cuts at: 0 1430 4026
Size: 1430 2596
SfcI C'TryA_G
Cuts at: 0 19 229 2347 3206 3752 3953 4026
Size: 19 210 2118 859 546 201 73
Fragments arranged by size:
2118 859 546 210 201 73 19
SfoI GGC'GCC
Cuts at: 0 2005 4026
Size: 2005 2021
SmaI CCC'GGG
Cuts at: 0 52 3447 3517 4026
Size: 52 3395 70 509
Fragments arranged by size:
3395 509 70 52
SmlI C'TyrA_G
Cuts at: 0 187 949 1032 1619 2473 2524 2638 3649
Size: 187 762 83 587 854 51 114 1011
Cuts at: 3649 4026
Size: 377
Fragments arranged by size:
1011 854 762 587 377 187 114 83
51
SnaBI TAC'GTA
Cuts at: 0 3940 4026
Size: 3940 86
SspI AAT'ATT
Cuts at: 0 2365 4026
Size: 2365 1661
StyI C'CwwG_G
Cuts at: 0 1412 1673 1770 1779 1787 2456 2976 3634
Size: 1412 261 97 9 8 669 520 658
Cuts at: 3634 3833 4004 4026
Size: 199 171 22
Fragments arranged by size:
1412 669 658 520 261 199 171 97
22 9 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 521 1867 2547 3269 4026
Size: 521 1346 680 722 757
Fragments arranged by size:
1346 757 722 680 521
TatI w'GTAC_w
Cuts at: 0 146 2445 2465 3668 4026
Size: 146 2299 20 1203 358
Fragments arranged by size:
2299 1203 358 146 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1943 1975 2676 2708 4026
Size: 1943 32 701 32 1318
Fragments arranged by size:
1943 1318 701 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 977 4026
Size: 977 3049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 331 1738 2292 3181 4026
Size: 306 25 1407 554 889 845
Fragments arranged by size:
1407 889 845 554 306 25
XhoI C'TCGA_G
Cuts at: 0 1032 1619 4026
Size: 1032 587 2407
Fragments arranged by size:
2407 1032 587
XmaI C'CCGG_G
Cuts at: 0 50 3445 3515 4026
Size: 50 3395 70 511
Fragments arranged by size:
3395 511 70 50
XmnI GAAnn'nnTTC
Cuts at: 0 277 4026
Size: 277 3749
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI ApaLI
ApoI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KasI MfeI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SanDI SapI SfcI SfoI SmaI SmlI SnaBI
SspI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AscI AseI AsiSI BfrBI BmgBI BmtI BplI
BsaBI BsiWI BsmBI BssHII BstBI BstZ17I ClaI DrdI
EcoRV FseI FspI FspAI HpaI KpnI MluI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PsiI
PsrI PvuI RsrII SacII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SpeI SphI SrfI StuI SwaI
XbaI ZraI