Restriction map for
pFN21AE3446
(Linear) (Six-base) MAPSORT of: KIEE3446.seq Check: 9528 from: 1 to: 1161
KIEE3446.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AccI GT'mk_AC
Cuts at: 0 368 1161
Size: 368 793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 522 1161
Size: 508 14 639
Fragments arranged by size:
639 508 14
AleI CACnn'nnGTG
Cuts at: 0 358 1161
Size: 358 803
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 543 1161
Size: 543 618
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 808 840 1161
Size: 400 32 376 32 321
Fragments arranged by size:
400 376 321 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 214 545 1161
Size: 214 331 616
Fragments arranged by size:
616 331 214
ApaI G_GGCC'C
Cuts at: 0 1078 1161
Size: 1078 83
BamHI G'GATC_C
Cuts at: 0 752 1161
Size: 752 409
BanI G'GyrC_C
Cuts at: 0 8 1161
Size: 8 1153
BanII G_rGCy'C
Cuts at: 0 992 1063 1078 1161
Size: 992 71 15 83
Fragments arranged by size:
992 83 71 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 966 1000 1161
Size: 966 34 161
Fragments arranged by size:
966 161 34
BclI T'GATC_A
Cuts at: 0 227 923 1161
Size: 227 696 238
Fragments arranged by size:
696 238 227
BglII A'GATC_T
Cuts at: 0 413 1161
Size: 413 748
BlpI GC'TnA_GC
Cuts at: 0 621 1161
Size: 621 540
Bme1580I G_kGCm'C
Cuts at: 0 787 1078 1161
Size: 787 291 83
Fragments arranged by size:
787 291 83
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 604 657 873 922 1007 1153 1161
Size: 127 477 53 216 49 85 146 8
Fragments arranged by size:
477 216 146 127 85 53 49 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 1161
Size: 151 1010
BsaBI GATnn'nnATC
Cuts at: 0 757 1161
Size: 757 404
BsaWI w'CCGG_w
Cuts at: 0 557 1045 1161
Size: 557 488 116
Fragments arranged by size:
557 488 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 400 430 871 898 901 928 1126 1156
Size: 400 30 441 27 3 27 198 30
Cuts at: 1156 1161
Size: 5
Fragments arranged by size:
441 400 198 30 30 27 27 5
3
BseYI C'CCAG_C
Cuts at: 0 736 1161
Size: 736 425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1161
Size: 757 404
BsiHKAI G_wGCw'C
Cuts at: 0 311 992 1161
Size: 311 681 169
Fragments arranged by size:
681 311 169
Bsp1286I G_dGCh'C
Cuts at: 0 311 787 992 1063 1078 1161
Size: 311 476 205 71 15 83
Fragments arranged by size:
476 311 205 83 71 15
BsrBI CCG'CTC
Cuts at: 0 809 1161
Size: 809 352
BsrDI GCAATG_nn'
Cuts at: 0 74 138 1161
Size: 74 64 1023
Fragments arranged by size:
1023 74 64
BsrFI r'CCGG_y
Cuts at: 0 93 299 662 1161
Size: 93 206 363 499
Fragments arranged by size:
499 363 206 93
BssSI C'ACGA_G
Cuts at: 0 978 1161
Size: 978 183
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 1161
Size: 15 1146
BstYI r'GATC_y
Cuts at: 0 413 752 1161
Size: 413 339 409
Fragments arranged by size:
413 409 339
BtgI C'CryG_G
Cuts at: 0 872 1054 1161
Size: 872 182 107
Fragments arranged by size:
872 182 107
EaeI y'GGCC_r
Cuts at: 0 707 1037 1161
Size: 707 330 124
Fragments arranged by size:
707 330 124
EarI CTCTTCn'nnn_
Cuts at: 0 316 1161
Size: 316 845
EcoICRI GAG'CTC
Cuts at: 0 990 1161
Size: 990 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 508 522 604 657 873 922 1007
Size: 127 381 14 82 53 216 49 85
Cuts at: 1007 1153 1161
Size: 146 8
Fragments arranged by size:
381 216 146 127 85 82 53 49
14 8
EcoO109I rG'GnC_Cy
Cuts at: 0 999 1161
Size: 999 162
HaeII r_GCGC'y
Cuts at: 0 1022 1161
Size: 1022 139
MfeI C'AATT_G
Cuts at: 0 420 1161
Size: 420 741
MscI TGG'CCA
Cuts at: 0 709 1039 1161
Size: 709 330 122
Fragments arranged by size:
709 330 122
MslI CAynn'nnrTG
Cuts at: 0 358 700 703 850 1161
Size: 358 342 3 147 311
Fragments arranged by size:
358 342 311 147 3
MspA1I CmG'CkG
Cuts at: 0 932 1161
Size: 932 229
NaeI GCC'GGC
Cuts at: 0 301 1161
Size: 301 860
NcoI C'CATG_G
Cuts at: 0 1054 1161
Size: 1054 107
NgoMIV G'CCGG_C
Cuts at: 0 299 1161
Size: 299 862
NspI r_CATG'y
Cuts at: 0 670 864 1161
Size: 670 194 297
Fragments arranged by size:
670 297 194
PasI CC'CwG_GG
Cuts at: 0 600 1161
Size: 600 561
PfoI T'CCnGG_A
Cuts at: 0 172 581 1161
Size: 172 409 580
Fragments arranged by size:
580 409 172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 400 432 1161
Size: 400 32 729
Fragments arranged by size:
729 400 32
PpuMI rG'GwC_Cy
Cuts at: 0 999 1161
Size: 999 162
PspOMI G'GGCC_C
Cuts at: 0 1074 1161
Size: 1074 87
PstI C_TGCA'G
Cuts at: 0 20 1161
Size: 20 1141
SacI G_AGCT'C
Cuts at: 0 992 1161
Size: 992 169
SanDI GG'GwC_CC
Cuts at: 0 999 1161
Size: 999 162
SapI GCTCTTCn'nnn_
Cuts at: 0 316 1161
Size: 316 845
SfcI C'TryA_G
Cuts at: 0 16 1161
Size: 16 1145
SgrAI Cr'CCGG_yG
Cuts at: 0 93 1161
Size: 93 1068
SmlI C'TyrA_G
Cuts at: 0 166 1161
Size: 166 995
SphI G_CATG'C
Cuts at: 0 670 864 1161
Size: 670 194 297
Fragments arranged by size:
670 297 194
StyI C'CwwG_G
Cuts at: 0 80 1054 1161
Size: 80 974 107
Fragments arranged by size:
974 107 80
TatI w'GTAC_w
Cuts at: 0 878 1161
Size: 878 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 154 186 1161
Size: 154 32 975
Fragments arranged by size:
975 154 32
Tth111I GACn'n_nGTC
Cuts at: 0 1109 1161
Size: 1109 52
Enzymes that do cut:
AccI AcuI AleI AlfI AloI AlwNI ApaI BamHI
BanI BanII BcgI BclI BglII BlpI Bme1580I BpmI
BpuEI BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI Bsp1286I
BsrBI BsrDI BsrFI BssSI BstAPI BstYI BtgI EaeI
EarI EcoICRI Eco57MI EcoO109I HaeII MfeI MscI MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PfoI PpiI
PpuMI PspOMI PstI SacI SanDI SapI SfcI SgrAI
SmlI SphI StyI TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbsI BbvCI BciVI BfrBI BglI BmgBI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI BseRI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI
BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MluI MmeI NarI NdeI
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII
XbaI XcmI XhoI XmaI XmnI ZraI