Restriction map for
pFN21AE3442
(Linear) (Six-base) MAPSORT of: KIEE3442.seq Check: 1596 from: 1 to: 3963
KIEE3442.orf
With 182 enzymes: SgfI *
October 21, 2016 17:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1491 3963
Size: 1491 2472
Acc65I G'GTAC_C
Cuts at: 0 2892 3587 3963
Size: 2892 695 376
Fragments arranged by size:
2892 695 376
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1388 1714 2104 2208 3924 3963
Size: 676 712 326 390 104 1716 39
Fragments arranged by size:
1716 712 676 390 326 104 39
AflIII A'CryG_T
Cuts at: 0 3892 3963
Size: 3892 71
AgeI A'CCGG_T
Cuts at: 0 3388 3963
Size: 3388 575
AleI CACnn'nnGTG
Cuts at: 0 1124 3963
Size: 1124 2839
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2397 2417 2612 3963
Size: 2397 20 195 1351
Fragments arranged by size:
2397 1351 195 20
AlwNI CAG_nnn'CTG
Cuts at: 0 654 810 1089 1255 1689 1692 2850 3051
Size: 654 156 279 166 434 3 1158 201
Cuts at: 3051 3183 3963
Size: 132 780
Fragments arranged by size:
1158 780 654 434 279 201 166 156
132 3
ApaI G_GGCC'C
Cuts at: 0 65 725 819 1633 3633 3963
Size: 65 660 94 814 2000 330
Fragments arranged by size:
2000 814 660 330 94 65
ApaLI G'TGCA_C
Cuts at: 0 2801 3963
Size: 2801 1162
ApoI r'AATT_y
Cuts at: 0 576 1332 2063 2078 3963
Size: 576 756 731 15 1885
Fragments arranged by size:
1885 756 731 576 15
AvaI C'yCGr_G
Cuts at: 0 1202 2696 3583 3626 3963
Size: 1202 1494 887 43 337
Fragments arranged by size:
1494 1202 887 337 43
BanI G'GyrC_C
Cuts at: 0 775 2570 2892 3498 3587 3963
Size: 775 1795 322 606 89 376
Fragments arranged by size:
1795 775 606 376 322 89
BanII G_rGCy'C
Cuts at: 0 65 725 819 1209 1633 1774 1810 3584
Size: 65 660 94 390 424 141 36 1774
Cuts at: 3584 3633 3963
Size: 49 330
Fragments arranged by size:
1774 660 424 390 330 141 94 65
49 36
BbsI GAAGACnn'nnnn_
Cuts at: 0 664 2023 3755 3963
Size: 664 1359 1732 208
Fragments arranged by size:
1732 1359 664 208
BbvCI CC'TCA_GC
Cuts at: 0 2102 2560 3963
Size: 2102 458 1403
Fragments arranged by size:
2102 1403 458
BciVI GTATCCnnnnn_n'
Cuts at: 0 3443 3963
Size: 3443 520
BclI T'GATC_A
Cuts at: 0 1116 1289 3963
Size: 1116 173 2674
Fragments arranged by size:
2674 1116 173
BfrBI ATG'CAT
Cuts at: 0 3270 3963
Size: 3270 693
BglI GCCn_nnn'nGGC
Cuts at: 0 1637 3963
Size: 1637 2326
BglII A'GATC_T
Cuts at: 0 929 1443 3963
Size: 929 514 2520
Fragments arranged by size:
2520 929 514
BlpI GC'TnA_GC
Cuts at: 0 2452 3913 3963
Size: 2452 1461 50
Fragments arranged by size:
2452 1461 50
Bme1580I G_kGCm'C
Cuts at: 0 65 725 780 819 1633 2573 2805 3201
Size: 65 660 55 39 814 940 232 396
Cuts at: 3201 3503 3633 3963
Size: 302 130 330
Fragments arranged by size:
940 814 660 396 330 302 232 130
65 55 39
BmgBI CAC'GTC
Cuts at: 0 496 2831 3963
Size: 496 2335 1132
Fragments arranged by size:
2335 1132 496
BmrI ACTGGGnnnn_n'
Cuts at: 0 59 2228 2292 2504 2792 3963
Size: 59 2169 64 212 288 1171
Fragments arranged by size:
2169 1171 288 212 64 59
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 772 1788 1872 3107 3312 3963
Size: 772 1016 84 1235 205 651
Fragments arranged by size:
1235 1016 772 651 205 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 759 1493 2032 2053 2149 2466 3530 3619
Size: 759 734 539 21 96 317 1064 89
Cuts at: 3619 3963
Size: 344
Fragments arranged by size:
1064 759 734 539 344 317 96 89
21
Bpu10I CC'TnA_GC
Cuts at: 0 2102 2560 3963
Size: 2102 458 1403
Fragments arranged by size:
2102 1403 458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1591 3402 3963
Size: 1591 1811 561
Fragments arranged by size:
1811 1591 561
BsaAI yAC'GTr
Cuts at: 0 762 3893 3963
Size: 762 3131 70
Fragments arranged by size:
3131 762 70
BsaBI GATnn'nnATC
Cuts at: 0 593 3963
Size: 593 3370
BsaHI Gr'CG_yC
Cuts at: 0 962 3963
Size: 962 3001
BsaWI w'CCGG_w
Cuts at: 0 3388 3963
Size: 3388 575
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1807 1837 2778 2808 3963
Size: 1807 30 941 30 1155
Fragments arranged by size:
1807 1155 941 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1410 1673 1780 2090 2351 3301 3864 3963
Size: 1410 263 107 310 261 950 563 99
Fragments arranged by size:
1410 950 563 310 263 261 107 99
BseYI C'CCAG_C
Cuts at: 0 102 150 299 1682 2021 2707 3094 3176
Size: 102 48 149 1383 339 686 387 82
Cuts at: 3176 3502 3591 3712 3963
Size: 326 89 121 251
Fragments arranged by size:
1383 686 387 339 326 251 149 121
102 89 82 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1303 1819 1822 2627 3963
Size: 671 632 516 3 805 1336
Fragments arranged by size:
1336 805 671 632 516 3
BsiEI CG_ry'CG
Cuts at: 0 134 207 3963
Size: 134 73 3756
Fragments arranged by size:
3756 134 73
BsiHKAI G_wGCw'C
Cuts at: 0 1188 1774 1810 2805 3617 3839 3963
Size: 1188 586 36 995 812 222 124
Fragments arranged by size:
1188 995 812 586 222 124 36
BsmI GAATG_Cn'
Cuts at: 0 1000 2928 3270 3481 3963
Size: 1000 1928 342 211 482
Fragments arranged by size:
1928 1000 482 342 211
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1183 3963
Size: 1183 2780
Bsp1286I G_dGCh'C
Cuts at: 0 65 725 780 819 1188 1209 1633 1774
Size: 65 660 55 39 369 21 424 141
Cuts at: 1774 1810 2573 2805 3201 3503 3584 3617 3633
Size: 36 763 232 396 302 81 33 16
Cuts at: 3633 3839 3963
Size: 206 124
Fragments arranged by size:
763 660 424 396 369 302 232 206
141 124 81 65 55 39 36 33
21 16
BspHI T'CATG_A
Cuts at: 0 3831 3963
Size: 3831 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1240 1491 2389 2406 2880 3963
Size: 1240 251 898 17 474 1083
Fragments arranged by size:
1240 1083 898 474 251 17
BsrBI CCG'CTC
Cuts at: 0 20 3963
Size: 20 3943
BsrFI r'CCGG_y
Cuts at: 0 133 1868 2938 3258 3388 3963
Size: 133 1735 1070 320 130 575
Fragments arranged by size:
1735 1070 575 320 133 130
BsrGI T'GTAC_A
Cuts at: 0 3675 3963
Size: 3675 288
BssSI C'ACGA_G
Cuts at: 0 3297 3706 3963
Size: 3297 409 257
Fragments arranged by size:
3297 409 257
BstAPI GCAn_nnn'nTGC
Cuts at: 0 810 1255 1551 2850 3963
Size: 810 445 296 1299 1113
Fragments arranged by size:
1299 1113 810 445 296
BstEII G'GTnAC_C
Cuts at: 0 2418 3963
Size: 2418 1545
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2550 3963
Size: 2550 1413
BstYI r'GATC_y
Cuts at: 0 900 929 1443 3963
Size: 900 29 514 2520
Fragments arranged by size:
2520 900 514 29
BtgI C'CryG_G
Cuts at: 0 65 165 468 920 1055 1702 3240 3963
Size: 65 100 303 452 135 647 1538 723
Fragments arranged by size:
1538 723 647 452 303 135 100 65
BtsI GCAGTG_nn'
Cuts at: 0 799 1087 1745 2265 2328 3563 3963
Size: 799 288 658 520 63 1235 400
Fragments arranged by size:
1235 799 658 520 400 288 63
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2714 2749 3963
Size: 2714 35 1214
Fragments arranged by size:
2714 1214 35
DraI TTT'AAA
Cuts at: 0 566 3869 3963
Size: 566 3303 94
Fragments arranged by size:
3303 566 94
DraIII CAC_nnn'GTG
Cuts at: 0 462 1407 3963
Size: 462 945 2556
Fragments arranged by size:
2556 945 462
DrdI GACnn_nn'nnGTC
Cuts at: 0 1092 3963
Size: 1092 2871
EaeI y'GGCC_r
Cuts at: 0 131 135 1052 1638 2552 2940 3963
Size: 131 4 917 586 914 388 1023
Fragments arranged by size:
1023 917 914 586 388 131 4
EagI C'GGCC_G
Cuts at: 0 131 3963
Size: 131 3832
EarI CTCTTCn'nnn_
Cuts at: 0 640 1349 2123 2227 3963
Size: 640 709 774 104 1736
Fragments arranged by size:
1736 774 709 640 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 74 871 1255 3963
Size: 74 797 384 2708
Fragments arranged by size:
2708 797 384 74
EcoICRI GAG'CTC
Cuts at: 0 1772 1808 3963
Size: 1772 36 2155
Fragments arranged by size:
2155 1772 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 759 1388 1493 1714 2032 2053 2104
Size: 676 83 629 105 221 318 21 51
Cuts at: 2104 2149 2208 2466 3530 3619 3924 3963
Size: 45 59 258 1064 89 305 39
Fragments arranged by size:
1064 676 629 318 305 258 221 105
89 83 59 51 45 39 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1036 2726 3518 3963
Size: 1036 1690 792 445
Fragments arranged by size:
1690 1036 792 445
EcoO109I rG'GnC_Cy
Cuts at: 0 98 1630 1708 2847 3402 3623 3630 3963
Size: 98 1532 78 1139 555 221 7 333
Fragments arranged by size:
1532 1139 555 333 221 98 78 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 243 2932 3336 3963
Size: 243 2689 404 627
Fragments arranged by size:
2689 627 404 243
FseI GG_CCGG'CC
Cuts at: 0 137 3963
Size: 137 3826
HaeII r_GCGC'y
Cuts at: 0 915 1701 3126 3963
Size: 915 786 1425 837
Fragments arranged by size:
1425 915 837 786
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 399 431 1652 1684 2259 2291 3394 3426
Size: 399 32 1221 32 575 32 1103 32
Cuts at: 3426 3500 3532 3963
Size: 74 32 431
Fragments arranged by size:
1221 1103 575 431 399 74 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1479 2445 3963
Size: 1479 966 1518
Fragments arranged by size:
1518 1479 966
KpnI G_GTAC'C
Cuts at: 0 2896 3591 3963
Size: 2896 695 372
Fragments arranged by size:
2896 695 372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 523 1695 1850 2770 3722 3963
Size: 478 45 1172 155 920 952 241
Fragments arranged by size:
1172 952 920 478 241 155 45
MscI TGG'CCA
Cuts at: 0 1054 2554 3963
Size: 1054 1500 1409
Fragments arranged by size:
1500 1409 1054
MslI CAynn'nnrTG
Cuts at: 0 1124 3204 3245 3458 3891 3963
Size: 1124 2080 41 213 433 72
Fragments arranged by size:
2080 1124 433 213 72 41
MspA1I CmG'CkG
Cuts at: 0 67 115 167 303 523 651 1287 1614
Size: 67 48 52 136 220 128 636 327
Cuts at: 1614 1689 1950 1971 2473 2592 3681 3963
Size: 75 261 21 502 119 1089 282
Fragments arranged by size:
1089 636 502 327 282 261 220 136
128 119 75 67 52 48 21
NaeI GCC'GGC
Cuts at: 0 135 3260 3963
Size: 135 3125 703
Fragments arranged by size:
3125 703 135
NcoI C'CATG_G
Cuts at: 0 468 920 1055 3963
Size: 468 452 135 2908
Fragments arranged by size:
2908 468 452 135
NdeI CA'TA_TG
Cuts at: 0 887 2033 3963
Size: 887 1146 1930
Fragments arranged by size:
1930 1146 887
NgoMIV G'CCGG_C
Cuts at: 0 133 3258 3963
Size: 133 3125 705
Fragments arranged by size:
3125 705 133
NsiI A_TGCA'T
Cuts at: 0 3272 3963
Size: 3272 691
NspI r_CATG'y
Cuts at: 0 1200 1681 2132 2451 2463 3963
Size: 1200 481 451 319 12 1500
Fragments arranged by size:
1500 1200 481 451 319 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 393 462 790 891 1311 3444 3963
Size: 393 69 328 101 420 2133 519
Fragments arranged by size:
2133 519 420 393 328 101 69
PfoI T'CCnGG_A
Cuts at: 0 332 1470 3546 3963
Size: 332 1138 2076 417
Fragments arranged by size:
2076 1138 417 332
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2341 2373 3963
Size: 2341 32 1590
Fragments arranged by size:
2341 1590 32
PpuMI rG'GwC_Cy
Cuts at: 0 98 1708 3402 3963
Size: 98 1610 1694 561
Fragments arranged by size:
1694 1610 561 98
PspOMI G'GGCC_C
Cuts at: 0 61 721 815 1629 3629 3963
Size: 61 660 94 814 2000 334
Fragments arranged by size:
2000 814 660 334 94 61
PstI C_TGCA'G
Cuts at: 0 806 1094 1451 1502 1955 1976 2478 3963
Size: 806 288 357 51 453 21 502 1485
Fragments arranged by size:
1485 806 502 453 357 288 51 21
PvuII CAG'CTG
Cuts at: 0 303 523 651 1287 1614 1689 1950 1971
Size: 303 220 128 636 327 75 261 21
Cuts at: 1971 2473 2592 3681 3963
Size: 502 119 1089 282
Fragments arranged by size:
1089 636 502 327 303 282 261 220
128 119 75 21
SacI G_AGCT'C
Cuts at: 0 1774 1810 3963
Size: 1774 36 2153
Fragments arranged by size:
2153 1774 36
SacII CC_GC'GG
Cuts at: 0 68 168 3963
Size: 68 100 3795
Fragments arranged by size:
3795 100 68
SanDI GG'GwC_CC
Cuts at: 0 98 1708 3402 3963
Size: 98 1610 1694 561
Fragments arranged by size:
1694 1610 561 98
SapI GCTCTTCn'nnn_
Cuts at: 0 1349 3963
Size: 1349 2614
SbfI CC_TGCA'GG
Cuts at: 0 1502 3963
Size: 1502 2461
SexAI A'CCwGG_T
Cuts at: 0 386 458 661 3963
Size: 386 72 203 3302
Fragments arranged by size:
3302 386 203 72
SfcI C'TryA_G
Cuts at: 0 802 1090 1122 1134 1447 1498 1594 1609
Size: 802 288 32 12 313 51 96 15
Cuts at: 1609 1951 1972 2474 2715 2839 3963
Size: 342 21 502 241 124 1124
Fragments arranged by size:
1124 802 502 342 313 288 241 124
96 51 32 21 15 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1637 3963
Size: 1637 2326
SmaI CCC'GGG
Cuts at: 0 2698 3628 3963
Size: 2698 930 335
Fragments arranged by size:
2698 930 335
SmlI C'TyrA_G
Cuts at: 0 1570 3381 3963
Size: 1570 1811 582
Fragments arranged by size:
1811 1570 582
SphI G_CATG'C
Cuts at: 0 1200 1681 3963
Size: 1200 481 2282
Fragments arranged by size:
2282 1200 481
SspI AAT'ATT
Cuts at: 0 449 3963
Size: 449 3514
StuI AGG'CCT
Cuts at: 0 1900 3963
Size: 1900 2063
StyI C'CwwG_G
Cuts at: 0 263 269 468 744 920 1055 1635 3069
Size: 263 6 199 276 176 135 580 1434
Cuts at: 3069 3099 3311 3963
Size: 30 212 652
Fragments arranged by size:
1434 652 580 276 263 212 199 176
135 30 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1399 3243 3854 3963
Size: 1399 1844 611 109
Fragments arranged by size:
1844 1399 611 109
TatI w'GTAC_w
Cuts at: 0 1574 3343 3675 3963
Size: 1574 1769 332 288
Fragments arranged by size:
1769 1574 332 288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2778 2810 3283 3315 3542 3574 3963
Size: 2778 32 473 32 227 32 389
Fragments arranged by size:
2778 473 389 227 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 3963
Size: 1062 2901
XmaI C'CCGG_G
Cuts at: 0 2696 3626 3963
Size: 2696 930 337
Fragments arranged by size:
2696 930 337
XmnI GAAnn'nnTTC
Cuts at: 0 328 2635 3963
Size: 328 2307 1328
Fragments arranged by size:
2307 1328 328
Enzymes that do cut:
AarI Acc65I AcuI AflIII AgeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FseI HaeII Hin4I HincII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NsiI NspI PflMI PfoI PpiI PpuMI PspOMI PstI
PvuII SacI SacII SanDI SapI SbfI SexAI SfcI
SfiI SmaI SmlI SphI SspI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AhdI AloI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BmtI
BsaI BsiWI BspEI BsrDI BssHII BstBI BstZ17I Bsu36I
BtgZI ClaI EcoRI EcoRV FspI FspAI HindIII HpaI
KasI MfeI MluI NarI NheI NotI NruI PacI
PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI
PvuI RsrII SalI ScaI SfoI SgfI SgrAI SnaBI
SpeI SrfI SwaI Tth111I XbaI XhoI ZraI