Restriction map for
pFN21AE3441
(Linear) (Six-base) MAPSORT of: KIEE3441.seq Check: 8006 from: 1 to: 3882
KIEE3441.orf
With 182 enzymes: SgfI *
March 22, 2012 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 954 1997 3882
Size: 954 1043 1885
Fragments arranged by size:
1885 1043 954
AccI GT'mk_AC
Cuts at: 0 2276 3882
Size: 2276 1606
AclI AA'CG_TT
Cuts at: 0 71 3882
Size: 71 3811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 679 956 1087 1145 2253 2896 3227
Size: 593 86 277 131 58 1108 643 331
Cuts at: 3227 3882
Size: 655
Fragments arranged by size:
1108 655 643 593 331 277 131 86
58
AflIII A'CryG_T
Cuts at: 0 2792 3882
Size: 2792 1090
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2124 2666 3882
Size: 2124 542 1216
Fragments arranged by size:
2124 1216 542
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2373 2405 3204 3236 3882
Size: 2373 32 799 32 646
Fragments arranged by size:
2373 799 646 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 99 391 2320 2691 3882
Size: 99 292 1929 371 1191
Fragments arranged by size:
1929 1191 371 292 99
ApaI G_GGCC'C
Cuts at: 0 2634 3882
Size: 2634 1248
ApaLI G'TGCA_C
Cuts at: 0 1823 3691 3882
Size: 1823 1868 191
Fragments arranged by size:
1868 1823 191
ApoI r'AATT_y
Cuts at: 0 145 1045 1348 3023 3882
Size: 145 900 303 1675 859
Fragments arranged by size:
1675 900 859 303 145
AseI AT'TA_AT
Cuts at: 0 1481 3882
Size: 1481 2401
AvaI C'yCGr_G
Cuts at: 0 1542 3882
Size: 1542 2340
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1698 1731 2025 2058 3882
Size: 1698 33 294 33 1824
Fragments arranged by size:
1824 1698 294 33 33
BanI G'GyrC_C
Cuts at: 0 675 2494 2604 3574 3597 3882
Size: 675 1819 110 970 23 285
Fragments arranged by size:
1819 970 675 285 110 23
BanII G_rGCy'C
Cuts at: 0 410 574 1474 1543 2258 2366 2634 3882
Size: 410 164 900 69 715 108 268 1248
Fragments arranged by size:
1248 900 715 410 268 164 108 69
BbeI G_GCGC'C
Cuts at: 0 2608 3882
Size: 2608 1274
BciVI GTATCCnnnnn_n'
Cuts at: 0 2657 3882
Size: 2657 1225
BglI GCCn_nnn'nGGC
Cuts at: 0 2551 3882
Size: 2551 1331
BglII A'GATC_T
Cuts at: 0 750 3882
Size: 750 3132
BlpI GC'TnA_GC
Cuts at: 0 812 2918 3086 3882
Size: 812 2106 168 796
Fragments arranged by size:
2106 812 796 168
Bme1580I G_kGCm'C
Cuts at: 0 1286 1827 2634 3695 3882
Size: 1286 541 807 1061 187
Fragments arranged by size:
1286 1061 807 541 187
BmrI ACTGGGnnnn_n'
Cuts at: 0 305 568 2679 3882
Size: 305 263 2111 1203
Fragments arranged by size:
2111 1203 305 263
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 3882
Size: 1188 2694
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 368 502 671 1232 1554 2182 2276
Size: 121 247 134 169 561 322 628 94
Cuts at: 2276 3197 3399 3882
Size: 921 202 483
Fragments arranged by size:
921 628 561 483 322 247 202 169
134 121 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 3441 3650 3882
Size: 431 3010 209 232
Fragments arranged by size:
3010 431 232 209
BsaI GGTCTCn'nnnn_
Cuts at: 0 3249 3882
Size: 3249 633
BsaAI yAC'GTr
Cuts at: 0 3429 3882
Size: 3429 453
BsaHI Gr'CG_yC
Cuts at: 0 2482 2605 3882
Size: 2482 123 1277
Fragments arranged by size:
2482 1277 123
BsaWI w'CCGG_w
Cuts at: 0 44 2713 3882
Size: 44 2669 1169
Fragments arranged by size:
2669 1169 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 2300 2330 3562 3592 3882
Size: 316 30 1954 30 1232 30 290
Fragments arranged by size:
1954 1232 316 290 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 410 1131 1915 2266 2892 2968 2997 3479
Size: 410 721 784 351 626 76 29 482
Cuts at: 3479 3882
Size: 403
Fragments arranged by size:
784 721 626 482 410 403 351 76
29
BseYI C'CCAG_C
Cuts at: 0 19 391 2639 3034 3882
Size: 19 372 2248 395 848
Fragments arranged by size:
2248 848 395 372 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 871 982 1175 1444 1822 2148 3792
Size: 444 427 111 193 269 378 326 1644
Cuts at: 3792 3882
Size: 90
Fragments arranged by size:
1644 444 427 378 326 269 193 111
90
BsiHKAI G_wGCw'C
Cuts at: 0 1543 1827 2103 2168 3695 3882
Size: 1543 284 276 65 1527 187
Fragments arranged by size:
1543 1527 284 276 187 65
BsmI GAATG_Cn'
Cuts at: 0 2772 3546 3882
Size: 2772 774 336
Fragments arranged by size:
2772 774 336
Bsp1286I G_dGCh'C
Cuts at: 0 410 574 1286 1474 1543 1827 2103 2168
Size: 410 164 712 188 69 284 276 65
Cuts at: 2168 2258 2366 2634 3695 3882
Size: 90 108 268 1061 187
Fragments arranged by size:
1061 712 410 284 276 268 188 187
164 108 90 69 65
BspEI T'CCGG_A
Cuts at: 0 44 3882
Size: 44 3838
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 954 1997 3267 3882
Size: 954 1043 1270 615
Fragments arranged by size:
1270 1043 954 615
BsrDI GCAATG_nn'
Cuts at: 0 2415 2955 3882
Size: 2415 540 927
Fragments arranged by size:
2415 927 540
BsrGI T'GTAC_A
Cuts at: 0 3336 3882
Size: 3336 546
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3141 3574 3779 3882
Size: 3141 433 205 103
Fragments arranged by size:
3141 433 205 103
BstYI r'GATC_y
Cuts at: 0 95 234 750 3180 3882
Size: 95 139 516 2430 702
Fragments arranged by size:
2430 702 516 139 95
Bsu36I CC'TnA_GG
Cuts at: 0 468 3164 3882
Size: 468 2696 718
Fragments arranged by size:
2696 718 468
BtgI C'CryG_G
Cuts at: 0 1644 3747 3882
Size: 1644 2103 135
Fragments arranged by size:
2103 1644 135
BtsI GCAGTG_nn'
Cuts at: 0 1427 2181 3882
Size: 1427 754 1701
Fragments arranged by size:
1701 1427 754
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 699 734 2778 2813 3882
Size: 699 35 2044 35 1069
Fragments arranged by size:
2044 1069 699 35 35
DraI TTT'AAA
Cuts at: 0 705 2837 3882
Size: 705 2132 1045
Fragments arranged by size:
2132 1045 705
DraIII CAC_nnn'GTG
Cuts at: 0 544 3882
Size: 544 3338
DrdI GACnn_nn'nnGTC
Cuts at: 0 2272 2487 3882
Size: 2272 215 1395
Fragments arranged by size:
2272 1395 215
EarI CTCTTCn'nnn_
Cuts at: 0 2069 2213 2329 2345 3882
Size: 2069 144 116 16 1537
Fragments arranged by size:
2069 1537 144 116 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2630 2792 3882
Size: 2630 162 1090
Fragments arranged by size:
2630 1090 162
EcoICRI GAG'CTC
Cuts at: 0 1541 3882
Size: 1541 2341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 368 502 593 671 679 956 1087
Size: 121 247 134 91 78 8 277 131
Cuts at: 1087 1145 1232 1554 2182 2253 2276 2896 3197
Size: 58 87 322 628 71 23 620 301
Cuts at: 3197 3227 3399 3882
Size: 30 172 483
Fragments arranged by size:
628 620 483 322 301 277 247 172
134 131 121 91 87 78 71 58
30 23 8
EcoO109I rG'GnC_Cy
Cuts at: 0 950 2630 2631 3622 3831 3882
Size: 950 1680 1 991 209 51
Fragments arranged by size:
1680 991 950 209 51 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 276 309 795 1581 3691 3882
Size: 276 33 486 786 2110 191
Fragments arranged by size:
2110 786 486 276 191 33
HaeII r_GCGC'y
Cuts at: 0 2608 2895 3882
Size: 2608 287 987
Fragments arranged by size:
2608 987 287
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 148 180 1525 1557 2137 2169 2499 2531
Size: 148 32 1345 32 580 32 330 32
Cuts at: 2531 2971 3003 3144 3176 3219 3251 3882
Size: 440 32 141 32 43 32 631
Fragments arranged by size:
1345 631 580 440 330 148 141 43
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 885 3882
Size: 885 2997
HindIII A'AGCT_T
Cuts at: 0 1127 1459 2812 3882
Size: 1127 332 1353 1070
Fragments arranged by size:
1353 1127 1070 332
KasI G'GCGC_C
Cuts at: 0 2604 3882
Size: 2604 1278
MfeI C'AATT_G
Cuts at: 0 555 2730 3882
Size: 555 2175 1152
Fragments arranged by size:
2175 1152 555
MluI A'CGCG_T
Cuts at: 0 2792 3882
Size: 2792 1090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3486 3882
Size: 3486 396
MslI CAynn'nnrTG
Cuts at: 0 1063 3882
Size: 1063 2819
MspA1I CmG'CkG
Cuts at: 0 388 1040 1364 2108 2140 2611 3502 3882
Size: 388 652 324 744 32 471 891 380
Fragments arranged by size:
891 744 652 471 388 380 324 32
NarI GG'CG_CC
Cuts at: 0 2605 3882
Size: 2605 1277
NcoI C'CATG_G
Cuts at: 0 1644 3747 3882
Size: 1644 2103 135
Fragments arranged by size:
2103 1644 135
NdeI CA'TA_TG
Cuts at: 0 3737 3778 3882
Size: 3737 41 104
Fragments arranged by size:
3737 104 41
NruI TCG'CGA
Cuts at: 0 1869 3882
Size: 1869 2013
NspI r_CATG'y
Cuts at: 0 229 3882
Size: 229 3653
PasI CC'CwG_GG
Cuts at: 0 3057 3882
Size: 3057 825
PflMI CCAn_nnn'nTGG
Cuts at: 0 99 391 3114 3322 3882
Size: 99 292 2723 208 560
Fragments arranged by size:
2723 560 292 208 99
PfoI T'CCnGG_A
Cuts at: 0 98 3183 3882
Size: 98 3085 699
Fragments arranged by size:
3085 699 98
PmlI CAC'GTG
Cuts at: 0 3429 3882
Size: 3429 453
PpuMI rG'GwC_Cy
Cuts at: 0 950 3622 3882
Size: 950 2672 260
Fragments arranged by size:
2672 950 260
PspOMI G'GGCC_C
Cuts at: 0 2630 3882
Size: 2630 1252
PspXI vC'TCGA_Gb
Cuts at: 0 1542 3882
Size: 1542 2340
PstI C_TGCA'G
Cuts at: 0 156 195 914 2600 3882
Size: 156 39 719 1686 1282
Fragments arranged by size:
1686 1282 719 156 39
PvuII CAG'CTG
Cuts at: 0 388 1364 2108 2140 2611 3502 3882
Size: 388 976 744 32 471 891 380
Fragments arranged by size:
976 891 744 471 388 380 32
SacI G_AGCT'C
Cuts at: 0 1543 3882
Size: 1543 2339
SbfI CC_TGCA'GG
Cuts at: 0 2600 3882
Size: 2600 1282
ScaI AGT'ACT
Cuts at: 0 3174 3663 3882
Size: 3174 489 219
Fragments arranged by size:
3174 489 219
SfcI C'TryA_G
Cuts at: 0 107 152 191 518 910 2091 2204 2596
Size: 107 45 39 327 392 1181 113 392
Cuts at: 2596 3239 3882
Size: 643 643
Fragments arranged by size:
1181 643 643 392 392 327 113 107
45 39
SfoI GGC'GCC
Cuts at: 0 2606 3882
Size: 2606 1276
SmlI C'TyrA_G
Cuts at: 0 446 1542 3420 3665 3882
Size: 446 1096 1878 245 217
Fragments arranged by size:
1878 1096 446 245 217
StuI AGG'CCT
Cuts at: 0 933 2832 3882
Size: 933 1899 1050
Fragments arranged by size:
1899 1050 933
StyI C'CwwG_G
Cuts at: 0 1017 1644 1770 2585 2672 3581 3747 3882
Size: 1017 627 126 815 87 909 166 135
Fragments arranged by size:
1017 909 815 627 166 135 126 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1127 2398 2473 2512 3284 3882
Size: 1127 1271 75 39 772 598
Fragments arranged by size:
1271 1127 772 598 75 39
TatI w'GTAC_w
Cuts at: 0 1920 3172 3248 3336 3661 3882
Size: 1920 1252 76 88 325 221
Fragments arranged by size:
1920 1252 325 221 88 76
XbaI T'CTAG_A
Cuts at: 0 1306 3528 3882
Size: 1306 2222 354
Fragments arranged by size:
2222 1306 354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3754 3882
Size: 3754 128
XhoI C'TCGA_G
Cuts at: 0 1542 3882
Size: 1542 2340
XmnI GAAnn'nnTTC
Cuts at: 0 1564 3192 3882
Size: 1564 1628 690
Fragments arranged by size:
1628 1564 690
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AseI AvaI BaeI BanI BanII
BbeI BciVI BglI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI
BsrGI BstAPI BstYI Bsu36I BtgI BtsI CspCI DraI
DraIII DrdI EarI EciI EcoICRI Eco57MI EcoO109I FalI
HaeII Hin4I HincII HindIII KasI MfeI MluI MmeI
MslI MspA1I NarI NcoI NdeI NruI NspI PasI
PflMI PfoI PmlI PpuMI PspOMI PspXI PstI PvuII
SacI SbfI ScaI SfcI SfoI SmlI StuI StyI
TaqII TatI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AleI AscI
AsiSI AvrII BamHI BbsI BbvCI BcgI BclI BfrBI
BmgBI BmtI Bpu10I BsaBI BsiEI BsiWI BsmBI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI ClaI EaeI EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HpaI KpnI MscI NaeI NgoMIV NheI
NotI NsiI PacI PciI PmeI PpiI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XmaI ZraI