Restriction map for
pFN21AE3436
(Linear) (Six-base) MAPSORT of: KIEE3436.seq Check: 816 from: 1 to: 2787
KIEE3436.orf
With 182 enzymes: SgfI *
March 22, 2012 16:34 ..
AccI GT'mk_AC
Cuts at: 0 105 1488 2787
Size: 105 1383 1299
Fragments arranged by size:
1383 1299 105
Acc65I G'GTAC_C
Cuts at: 0 1155 2787
Size: 1155 1632
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 506 1024 1115 2327 2787
Size: 460 46 518 91 1212 460
Fragments arranged by size:
1212 518 460 460 91 46
AflIII A'CryG_T
Cuts at: 0 906 2787
Size: 906 1881
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1607 1639 2787
Size: 1607 32 1148
Fragments arranged by size:
1607 1148 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2085 2787
Size: 2085 702
ApaLI G'TGCA_C
Cuts at: 0 2116 2787
Size: 2116 671
ApoI r'AATT_y
Cuts at: 0 91 226 634 660 1112 1135 1518 1601
Size: 91 135 408 26 452 23 383 83
Cuts at: 1601 1634 2301 2787
Size: 33 667 486
Fragments arranged by size:
667 486 452 408 383 135 91 83
33 26 23
AseI AT'TA_AT
Cuts at: 0 1222 2787
Size: 1222 1565
BanI G'GyrC_C
Cuts at: 0 1155 2787
Size: 1155 1632
BanII G_rGCy'C
Cuts at: 0 1400 2787
Size: 1400 1387
BbsI GAAGACnn'nnnn_
Cuts at: 0 670 2787
Size: 670 2117
BbvCI CC'TCA_GC
Cuts at: 0 1190 2787
Size: 1190 1597
BglII A'GATC_T
Cuts at: 0 2775 2787
Size: 2775 12
Bme1580I G_kGCm'C
Cuts at: 0 2120 2787
Size: 2120 667
BmrI ACTGGGnnnn_n'
Cuts at: 0 405 2787
Size: 405 2382
BmtI G_CTAG'C
Cuts at: 0 136 2762 2787
Size: 136 2626 25
Fragments arranged by size:
2626 136 25
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2483 2787
Size: 2483 304
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 1849 2787
Size: 407 1442 938
Fragments arranged by size:
1442 938 407
Bpu10I CC'TnA_GC
Cuts at: 0 1190 1533 2787
Size: 1190 343 1254
Fragments arranged by size:
1254 1190 343
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2597 2787
Size: 2597 190
BsaBI GATnn'nnATC
Cuts at: 0 953 2787
Size: 953 1834
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 278 1912 2600 2787
Size: 278 1634 688 187
Fragments arranged by size:
1634 688 278 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 1126 2787
Size: 1013 113 1661
Fragments arranged by size:
1661 1013 113
BsiHKAI G_wGCw'C
Cuts at: 0 1368 1400 2120 2787
Size: 1368 32 720 667
Fragments arranged by size:
1368 720 667 32
BsmI GAATG_Cn'
Cuts at: 0 1201 2787
Size: 1201 1586
Bsp1286I G_dGCh'C
Cuts at: 0 1368 1400 2120 2787
Size: 1368 32 720 667
Fragments arranged by size:
1368 720 667 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1626 2787
Size: 1626 1161
BsrDI GCAATG_nn'
Cuts at: 0 110 132 2769 2787
Size: 110 22 2637 18
Fragments arranged by size:
2637 110 22 18
BsrGI T'GTAC_A
Cuts at: 0 352 2787
Size: 352 2435
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2588 2787
Size: 2588 199
BstYI r'GATC_y
Cuts at: 0 865 1388 2775 2787
Size: 865 523 1387 12
Fragments arranged by size:
1387 865 523 12
Bsu36I CC'TnA_GG
Cuts at: 0 890 2787
Size: 890 1897
BtgI C'CryG_G
Cuts at: 0 1352 2252 2787
Size: 1352 900 535
Fragments arranged by size:
1352 900 535
BtsI GCAGTG_nn'
Cuts at: 0 516 1000 1983 2787
Size: 516 484 983 804
Fragments arranged by size:
983 804 516 484
DraI TTT'AAA
Cuts at: 0 2771 2787
Size: 2771 16
EaeI y'GGCC_r
Cuts at: 0 1697 2787
Size: 1697 1090
EarI CTCTTCn'nnn_
Cuts at: 0 19 2787
Size: 19 2768
EcoICRI GAG'CTC
Cuts at: 0 1398 2787
Size: 1398 1389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 460 506 1024 1115 1849 2327 2787
Size: 407 53 46 518 91 734 478 460
Fragments arranged by size:
734 518 478 460 407 91 53 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2612 2787
Size: 2612 175
EcoO109I rG'GnC_Cy
Cuts at: 0 654 2787
Size: 654 2133
EcoRV GAT'ATC
Cuts at: 0 1581 2787
Size: 1581 1206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 990 1810 2787
Size: 990 820 977
Fragments arranged by size:
990 977 820
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1429 1461 2164 2196 2787
Size: 1429 32 703 32 591
Fragments arranged by size:
1429 703 591 32 32
HincII GTy'rAC
Cuts at: 0 106 167 278 807 2787
Size: 106 61 111 529 1980
Fragments arranged by size:
1980 529 111 106 61
HpaI GTT'AAC
Cuts at: 0 278 2787
Size: 278 2509
KpnI G_GTAC'C
Cuts at: 0 1159 2787
Size: 1159 1628
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 824 958 1441 2382 2647 2787
Size: 294 530 134 483 941 265 140
Fragments arranged by size:
941 530 483 294 265 140 134
MslI CAynn'nnrTG
Cuts at: 0 509 1527 2167 2263 2506 2787
Size: 509 1018 640 96 243 281
Fragments arranged by size:
1018 640 509 281 243 96
MspA1I CmG'CkG
Cuts at: 0 49 1695 2787
Size: 49 1646 1092
Fragments arranged by size:
1646 1092 49
NcoI C'CATG_G
Cuts at: 0 1352 2252 2787
Size: 1352 900 535
Fragments arranged by size:
1352 900 535
NheI G'CTAG_C
Cuts at: 0 132 2758 2787
Size: 132 2626 29
Fragments arranged by size:
2626 132 29
NspI r_CATG'y
Cuts at: 0 910 2358 2787
Size: 910 1448 429
Fragments arranged by size:
1448 910 429
PciI A'CATG_T
Cuts at: 0 906 2787
Size: 906 1881
PfoI T'CCnGG_A
Cuts at: 0 1391 2787
Size: 1391 1396
PpuMI rG'GwC_Cy
Cuts at: 0 654 2787
Size: 654 2133
PsiI TTA'TAA
Cuts at: 0 440 2787
Size: 440 2347
PvuII CAG'CTG
Cuts at: 0 49 1695 2787
Size: 49 1646 1092
Fragments arranged by size:
1646 1092 49
SacI G_AGCT'C
Cuts at: 0 1400 2787
Size: 1400 1387
SalI G'TCGA_C
Cuts at: 0 104 2787
Size: 104 2683
SanDI GG'GwC_CC
Cuts at: 0 654 2787
Size: 654 2133
ScaI AGT'ACT
Cuts at: 0 30 2232 2787
Size: 30 2202 555
Fragments arranged by size:
2202 555 30
SfcI C'TryA_G
Cuts at: 0 2382 2787
Size: 2382 405
SmlI C'TyrA_G
Cuts at: 0 2612 2787
Size: 2612 175
SpeI A'CTAG_T
Cuts at: 0 58 2787
Size: 58 2729
SspI AAT'ATT
Cuts at: 0 694 1009 1917 2787
Size: 694 315 908 870
Fragments arranged by size:
908 870 694 315
StyI C'CwwG_G
Cuts at: 0 1352 1610 2252 2787
Size: 1352 258 642 535
Fragments arranged by size:
1352 642 535 258
TatI w'GTAC_w
Cuts at: 0 28 352 1569 1654 2230 2787
Size: 28 324 1217 85 576 557
Fragments arranged by size:
1217 576 557 324 85 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2229 2261 2787
Size: 2229 32 526
Fragments arranged by size:
2229 526 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 339 1692 2418 2787
Size: 339 1353 726 369
Fragments arranged by size:
1353 726 369 339
Enzymes that do cut:
AccI Acc65I AcuI AflIII AloI AlwNI ApaLI ApoI
AseI BanI BanII BbsI BbvCI BglII Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaBI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrGI BstXI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI Hin4I HincII HpaI KpnI
MmeI MslI MspA1I NcoI NheI NspI PciI PfoI
PpuMI PsiI PvuII SacI SalI SanDI ScaI SfcI
SmlI SpeI SspI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI ApaI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoRI FseI FspI FspAI HaeII HindIII
KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PstI PvuI RsrII
SacII SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI