Restriction map for
pFN21AE3435
(Linear) (Six-base) MAPSORT of: KIEE3435.seq Check: 1151 from: 1 to: 2781
KIEE3435.orf
With 182 enzymes: SgfI *
March 22, 2012 16:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 456 1567 2257 2781
Size: 456 1111 690 524
Fragments arranged by size:
1111 690 524 456
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 540 1028 2781
Size: 448 92 488 1753
Fragments arranged by size:
1753 488 448 92
AflII C'TTAA_G
Cuts at: 0 2301 2781
Size: 2301 480
AflIII A'CryG_T
Cuts at: 0 729 1144 2781
Size: 729 415 1637
Fragments arranged by size:
1637 729 415
AgeI A'CCGG_T
Cuts at: 0 2396 2781
Size: 2396 385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1101 2781
Size: 1101 1680
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 321 353 2781
Size: 321 32 2428
Fragments arranged by size:
2428 321 32
AlwNI CAG_nnn'CTG
Cuts at: 0 274 448 937 1220 2781
Size: 274 174 489 283 1561
Fragments arranged by size:
1561 489 283 274 174
ApaLI G'TGCA_C
Cuts at: 0 278 725 2781
Size: 278 447 2056
Fragments arranged by size:
2056 447 278
ApoI r'AATT_y
Cuts at: 0 649 1280 1487 1620 1718 1763 2063 2781
Size: 649 631 207 133 98 45 300 718
Fragments arranged by size:
718 649 631 300 207 133 98 45
AseI AT'TA_AT
Cuts at: 0 662 2781
Size: 662 2119
BanI G'GyrC_C
Cuts at: 0 361 2781
Size: 361 2420
BbeI G_GCGC'C
Cuts at: 0 365 2781
Size: 365 2416
BbsI GAAGACnn'nnnn_
Cuts at: 0 1967 2105 2781
Size: 1967 138 676
Fragments arranged by size:
1967 676 138
BciVI GTATCCnnnnn_n'
Cuts at: 0 1895 2781
Size: 1895 886
BclI T'GATC_A
Cuts at: 0 1122 1386 2781
Size: 1122 264 1395
Fragments arranged by size:
1395 1122 264
BfrBI ATG'CAT
Cuts at: 0 633 1708 2541 2781
Size: 633 1075 833 240
Fragments arranged by size:
1075 833 633 240
BglII A'GATC_T
Cuts at: 0 432 2781
Size: 432 2349
Bme1580I G_kGCm'C
Cuts at: 0 282 729 2781
Size: 282 447 2052
Fragments arranged by size:
2052 447 282
BmgBI CAC'GTC
Cuts at: 0 1363 2781
Size: 1363 1418
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 381 2781
Size: 381 2400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1526 1865 1874 2150 2781
Size: 1526 339 9 276 631
Fragments arranged by size:
1526 631 339 276 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2246 2781
Size: 2246 535
BsaI GGTCTCn'nnnn_
Cuts at: 0 21 2233 2781
Size: 21 2212 548
Fragments arranged by size:
2212 548 21
BsaBI GATnn'nnATC
Cuts at: 0 2153 2781
Size: 2153 628
BsaHI Gr'CG_yC
Cuts at: 0 362 2781
Size: 362 2419
BsaWI w'CCGG_w
Cuts at: 0 23 1898 2396 2781
Size: 23 1875 498 385
Fragments arranged by size:
1875 498 385 23
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1847 1877 2781
Size: 1847 30 904
Fragments arranged by size:
1847 904 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1661 2781
Size: 1661 1120
BseYI C'CCAG_C
Cuts at: 0 107 122 1300 2781
Size: 107 15 1178 1481
Fragments arranged by size:
1481 1178 107 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 950 1046 2781
Size: 79 871 96 1735
Fragments arranged by size:
1735 871 96 79
BsiEI CG_ry'CG
Cuts at: 0 49 2781
Size: 49 2732
BsiHKAI G_wGCw'C
Cuts at: 0 282 729 2781
Size: 282 447 2052
Fragments arranged by size:
2052 447 282
BsmI GAATG_Cn'
Cuts at: 0 2262 2781
Size: 2262 519
Bsp1286I G_dGCh'C
Cuts at: 0 282 729 2781
Size: 282 447 2052
Fragments arranged by size:
2052 447 282
BspHI T'CATG_A
Cuts at: 0 1767 2781
Size: 1767 1014
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 456 1567 1908 2257 2326 2781
Size: 456 1111 341 349 69 455
Fragments arranged by size:
1111 456 455 349 341 69
BsrBI CCG'CTC
Cuts at: 0 210 228 2781
Size: 210 18 2553
Fragments arranged by size:
2553 210 18
BsrDI GCAATG_nn'
Cuts at: 0 1710 2129 2767 2781
Size: 1710 419 638 14
Fragments arranged by size:
1710 638 419 14
BsrFI r'CCGG_y
Cuts at: 0 2396 2781
Size: 2396 385
BsrGI T'GTAC_A
Cuts at: 0 1147 2695 2781
Size: 1147 1548 86
Fragments arranged by size:
1548 1147 86
BstAPI GCAn_nnn'nTGC
Cuts at: 0 406 448 2781
Size: 406 42 2333
Fragments arranged by size:
2333 406 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 584 692 1783 2781
Size: 584 108 1091 998
Fragments arranged by size:
1091 998 584 108
BstYI r'GATC_y
Cuts at: 0 432 522 2781
Size: 432 90 2259
Fragments arranged by size:
2259 432 90
Bsu36I CC'TnA_GG
Cuts at: 0 203 2781
Size: 203 2578
BtgI C'CryG_G
Cuts at: 0 1494 2781
Size: 1494 1287
BtsI GCAGTG_nn'
Cuts at: 0 1083 2781
Size: 1083 1698
EaeI y'GGCC_r
Cuts at: 0 46 1773 2781
Size: 46 1727 1008
Fragments arranged by size:
1727 1008 46
EagI C'GGCC_G
Cuts at: 0 46 2781
Size: 46 2735
EarI CTCTTCn'nnn_
Cuts at: 0 665 780 1527 1817 2184 2781
Size: 665 115 747 290 367 597
Fragments arranged by size:
747 665 597 367 290 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 125 161 182 2781
Size: 125 36 21 2599
Fragments arranged by size:
2599 125 36 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 540 1028 1526 1865 1874 2150 2781
Size: 448 92 488 498 339 9 276 631
Fragments arranged by size:
631 498 488 448 339 276 92 9
EcoO109I rG'GnC_Cy
Cuts at: 0 271 2781
Size: 271 2510
EcoRI G'AATT_C
Cuts at: 0 1718 2063 2781
Size: 1718 345 718
Fragments arranged by size:
1718 718 345
EcoRV GAT'ATC
Cuts at: 0 1971 2781
Size: 1971 810
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 841 2781
Size: 841 1940
HaeII r_GCGC'y
Cuts at: 0 365 2781
Size: 365 2416
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1640 1672 2781
Size: 1042 32 566 32 1109
Fragments arranged by size:
1109 1042 566 32 32
KasI G'GCGC_C
Cuts at: 0 361 2781
Size: 361 2420
MfeI C'AATT_G
Cuts at: 0 1745 2781
Size: 1745 1036
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1002 1212 2781
Size: 1002 210 1569
Fragments arranged by size:
1569 1002 210
MscI TGG'CCA
Cuts at: 0 1775 2781
Size: 1775 1006
MslI CAynn'nnrTG
Cuts at: 0 851 1073 1361 2593 2781
Size: 851 222 288 1232 188
Fragments arranged by size:
1232 851 288 222 188
MspA1I CmG'CkG
Cuts at: 0 198 2781
Size: 198 2583
NarI GG'CG_CC
Cuts at: 0 362 2781
Size: 362 2419
NcoI C'CATG_G
Cuts at: 0 1494 2781
Size: 1494 1287
NdeI CA'TA_TG
Cuts at: 0 1982 2590 2781
Size: 1982 608 191
Fragments arranged by size:
1982 608 191
NotI GC'GGCC_GC
Cuts at: 0 46 2781
Size: 46 2735
NsiI A_TGCA'T
Cuts at: 0 635 1710 2543 2781
Size: 635 1075 833 238
Fragments arranged by size:
1075 833 635 238
NspI r_CATG'y
Cuts at: 0 733 1148 1190 2781
Size: 733 415 42 1591
Fragments arranged by size:
1591 733 415 42
PciI A'CATG_T
Cuts at: 0 729 1144 2781
Size: 729 415 1637
Fragments arranged by size:
1637 729 415
PpuMI rG'GwC_Cy
Cuts at: 0 271 2781
Size: 271 2510
PsiI TTA'TAA
Cuts at: 0 961 2075 2781
Size: 961 1114 706
Fragments arranged by size:
1114 961 706
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1140 1172 2781
Size: 1140 32 1609
Fragments arranged by size:
1609 1140 32
PstI C_TGCA'G
Cuts at: 0 249 1164 2781
Size: 249 915 1617
Fragments arranged by size:
1617 915 249
ScaI AGT'ACT
Cuts at: 0 1886 2781
Size: 1886 895
SfcI C'TryA_G
Cuts at: 0 245 377 759 1160 1469 2550 2753 2781
Size: 245 132 382 401 309 1081 203 28
Fragments arranged by size:
1081 401 382 309 245 203 132 28
SfoI GGC'GCC
Cuts at: 0 363 2781
Size: 363 2418
SmlI C'TyrA_G
Cuts at: 0 2261 2301 2781
Size: 2261 40 480
Fragments arranged by size:
2261 480 40
SspI AAT'ATT
Cuts at: 0 2111 2318 2781
Size: 2111 207 463
Fragments arranged by size:
2111 463 207
StuI AGG'CCT
Cuts at: 0 1676 2781
Size: 1676 1105
StyI C'CwwG_G
Cuts at: 0 825 1494 2781
Size: 825 669 1287
Fragments arranged by size:
1287 825 669
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1851 2781
Size: 1851 930
TatI w'GTAC_w
Cuts at: 0 1141 1147 1884 2695 2781
Size: 1141 6 737 811 86
Fragments arranged by size:
1141 811 737 86 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1847 1879 1893 1925 1937 1969 2781
Size: 1847 32 14 32 12 32 812
Fragments arranged by size:
1847 812 32 32 32 14 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1501 2781
Size: 1501 1280
XmnI GAAnn'nnTTC
Cuts at: 0 488 2781
Size: 488 2293
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AlfI AloI AlwNI
ApaLI ApoI AseI BanI BbeI BbsI BciVI BclI
BfrBI BglII Bme1580I BmgBI BplI BpmI BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I KasI MfeI MmeI MscI MslI MspA1I
NarI NcoI NdeI NotI NsiI NspI PciI PpuMI
PsiI PsrI PstI ScaI SfcI SfoI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AhdI AleI ApaI
AscI AsiSI AvaI AvrII BaeI BamHI BanII BbvCI
BcgI BglI BlpI BmrI BmtI Bpu10I BsaAI BsiWI
BsmBI BspEI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI FseI
FspI FspAI HincII HindIII HpaI KpnI MluI NaeI
NgoMIV NheI NruI PacI PasI PflMI PfoI PmeI
PmlI PpiI PshAI PspOMI PspXI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XhoI XmaI ZraI