Restriction map for
pFN21AE3432
(Linear) (Six-base) MAPSORT of: KIEE3432.seq Check: 2785 from: 1 to: 2424
KIEE3432.orf
With 182 enzymes: SgfI *
March 22, 2012 16:33 ..
AccI GT'mk_AC
Cuts at: 0 1571 1808 2424
Size: 1571 237 616
Fragments arranged by size:
1571 616 237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 2424
Size: 368 2056
AflIII A'CryG_T
Cuts at: 0 1157 2424
Size: 1157 1267
AleI CACnn'nnGTG
Cuts at: 0 1216 2424
Size: 1216 1208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 83 2424
Size: 83 2341
AlwNI CAG_nnn'CTG
Cuts at: 0 313 1893 1935 2415 2424
Size: 313 1580 42 480 9
Fragments arranged by size:
1580 480 313 42 9
ApoI r'AATT_y
Cuts at: 0 114 731 1744 1779 2424
Size: 114 617 1013 35 645
Fragments arranged by size:
1013 645 617 114 35
AvaI C'yCGr_G
Cuts at: 0 290 500 1063 2424
Size: 290 210 563 1361
Fragments arranged by size:
1361 563 290 210
BamHI G'GATC_C
Cuts at: 0 518 713 993 1148 1401 2424
Size: 518 195 280 155 253 1023
Fragments arranged by size:
1023 518 280 253 195 155
BanI G'GyrC_C
Cuts at: 0 67 903 1128 1319 1605 2424
Size: 67 836 225 191 286 819
Fragments arranged by size:
836 819 286 225 191 67
BanII G_rGCy'C
Cuts at: 0 172 388 753 2424
Size: 172 216 365 1671
Fragments arranged by size:
1671 365 216 172
BbeI G_GCGC'C
Cuts at: 0 1323 2424
Size: 1323 1101
BbsI GAAGACnn'nnnn_
Cuts at: 0 347 1420 2424
Size: 347 1073 1004
Fragments arranged by size:
1073 1004 347
BbvCI CC'TCA_GC
Cuts at: 0 2327 2424
Size: 2327 97
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 379 413 2424
Size: 379 34 2011
Fragments arranged by size:
2011 379 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1870 2424
Size: 1870 554
BlpI GC'TnA_GC
Cuts at: 0 752 1244 2424
Size: 752 492 1180
Fragments arranged by size:
1180 752 492
Bme1580I G_kGCm'C
Cuts at: 0 1049 1082 1170 1610 2155 2424
Size: 1049 33 88 440 545 269
Fragments arranged by size:
1049 545 440 269 88 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 264 1394 1664 2081 2424
Size: 264 1130 270 417 343
Fragments arranged by size:
1130 417 343 270 264
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 360 2424
Size: 360 2064
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1086 1193 1441 1992 2424
Size: 671 415 107 248 551 432
Fragments arranged by size:
671 551 432 415 248 107
Bpu10I CC'TnA_GC
Cuts at: 0 2327 2424
Size: 2327 97
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 1237 2424
Size: 781 456 1187
Fragments arranged by size:
1187 781 456
BsaI GGTCTCn'nnnn_
Cuts at: 0 532 776 947 2424
Size: 532 244 171 1477
Fragments arranged by size:
1477 532 244 171
BsaHI Gr'CG_yC
Cuts at: 0 948 1235 1320 2424
Size: 948 287 85 1104
Fragments arranged by size:
1104 948 287 85
BsaWI w'CCGG_w
Cuts at: 0 923 2424
Size: 923 1501
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2328 2358 2424
Size: 2328 30 66
Fragments arranged by size:
2328 66 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 174 278 346 349 460 550 565 2424
Size: 174 104 68 3 111 90 15 1859
Fragments arranged by size:
1859 174 111 104 90 68 15 3
BseYI C'CCAG_C
Cuts at: 0 306 514 658 1075 1196 1281 2424
Size: 306 208 144 417 121 85 1143
Fragments arranged by size:
1143 417 306 208 144 121 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1515 2424
Size: 1515 909
BsiHKAI G_wGCw'C
Cuts at: 0 141 1452 2424
Size: 141 1311 972
Fragments arranged by size:
1311 972 141
BsmI GAATG_Cn'
Cuts at: 0 2142 2424
Size: 2142 282
Bsp1286I G_dGCh'C
Cuts at: 0 141 172 388 753 1049 1082 1170 1452
Size: 141 31 216 365 296 33 88 282
Cuts at: 1452 1610 2155 2424
Size: 158 545 269
Fragments arranged by size:
545 365 296 282 269 216 158 141
88 33 31
BspEI T'CCGG_A
Cuts at: 0 923 2424
Size: 923 1501
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 701 2424
Size: 701 1723
BsrBI CCG'CTC
Cuts at: 0 1146 1248 2424
Size: 1146 102 1176
Fragments arranged by size:
1176 1146 102
BsrDI GCAATG_nn'
Cuts at: 0 214 1727 2424
Size: 214 1513 697
Fragments arranged by size:
1513 697 214
BsrFI r'CCGG_y
Cuts at: 0 791 2169 2424
Size: 791 1378 255
Fragments arranged by size:
1378 791 255
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1086 1893 2265 2424
Size: 1086 807 372 159
Fragments arranged by size:
1086 807 372 159
BstXI CCAn_nnnn'nTGG
Cuts at: 0 253 1191 1315 1338 2424
Size: 253 938 124 23 1086
Fragments arranged by size:
1086 938 253 124 23
BstYI r'GATC_y
Cuts at: 0 518 713 804 993 1148 1401 2424
Size: 518 195 91 189 155 253 1023
Fragments arranged by size:
1023 518 253 195 189 155 91
Bsu36I CC'TnA_GG
Cuts at: 0 203 1817 2128 2213 2424
Size: 203 1614 311 85 211
Fragments arranged by size:
1614 311 211 203 85
BtgI C'CryG_G
Cuts at: 0 369 1610 2424
Size: 369 1241 814
Fragments arranged by size:
1241 814 369
BtsI GCAGTG_nn'
Cuts at: 0 324 1383 2424
Size: 324 1059 1041
Fragments arranged by size:
1059 1041 324
DrdI GACnn_nn'nnGTC
Cuts at: 0 195 576 2424
Size: 195 381 1848
Fragments arranged by size:
1848 381 195
EaeI y'GGCC_r
Cuts at: 0 809 1626 2424
Size: 809 817 798
Fragments arranged by size:
817 809 798
EarI CTCTTCn'nnn_
Cuts at: 0 323 527 551 593 865 1547 2424
Size: 323 204 24 42 272 682 877
Fragments arranged by size:
877 682 323 272 204 42 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 671 1086 1193 1441 1992 2424
Size: 368 303 415 107 248 551 432
Fragments arranged by size:
551 432 415 368 303 248 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 578 961 1459 2424
Size: 578 383 498 965
Fragments arranged by size:
965 578 498 383
EcoO109I rG'GnC_Cy
Cuts at: 0 265 375 583 612 2323 2424
Size: 265 110 208 29 1711 101
Fragments arranged by size:
1711 265 208 110 101 29
EcoRI G'AATT_C
Cuts at: 0 731 2424
Size: 731 1693
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1941 2424
Size: 1941 483
HaeII r_GCGC'y
Cuts at: 0 306 1323 1662 2424
Size: 306 1017 339 762
Fragments arranged by size:
1017 762 339 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 2424
Size: 418 32 1974
Fragments arranged by size:
1974 418 32
HincII GTy'rAC
Cuts at: 0 987 2424
Size: 987 1437
HindIII A'AGCT_T
Cuts at: 0 2000 2424
Size: 2000 424
HpaI GTT'AAC
Cuts at: 0 987 2424
Size: 987 1437
KasI G'GCGC_C
Cuts at: 0 1319 2424
Size: 1319 1105
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 2424
Size: 455 1969
MscI TGG'CCA
Cuts at: 0 811 1628 2424
Size: 811 817 796
Fragments arranged by size:
817 811 796
MslI CAynn'nnrTG
Cuts at: 0 1216 1313 2424
Size: 1216 97 1111
Fragments arranged by size:
1216 1111 97
MspA1I CmG'CkG
Cuts at: 0 253 756 1200 1893 2035 2412 2424
Size: 253 503 444 693 142 377 12
Fragments arranged by size:
693 503 444 377 253 142 12
NarI GG'CG_CC
Cuts at: 0 1320 2424
Size: 1320 1104
NspI r_CATG'y
Cuts at: 0 1089 1110 1161 2424
Size: 1089 21 51 1263
Fragments arranged by size:
1263 1089 51 21
PasI CC'CwG_GG
Cuts at: 0 999 2424
Size: 999 1425
PciI A'CATG_T
Cuts at: 0 1157 2424
Size: 1157 1267
PflMI CCAn_nnn'nTGG
Cuts at: 0 220 313 1713 2424
Size: 220 93 1400 711
Fragments arranged by size:
1400 711 220 93
PfoI T'CCnGG_A
Cuts at: 0 716 1151 2015 2424
Size: 716 435 864 409
Fragments arranged by size:
864 716 435 409
PpuMI rG'GwC_Cy
Cuts at: 0 265 375 2424
Size: 265 110 2049
Fragments arranged by size:
2049 265 110
PshAI GACnn'nnGTC
Cuts at: 0 270 2424
Size: 270 2154
PspXI vC'TCGA_Gb
Cuts at: 0 290 2424
Size: 290 2134
PstI C_TGCA'G
Cuts at: 0 102 620 1094 1378 1898 2424
Size: 102 518 474 284 520 526
Fragments arranged by size:
526 520 518 474 284 102
PvuII CAG'CTG
Cuts at: 0 253 1200 1893 2035 2412 2424
Size: 253 947 693 142 377 12
Fragments arranged by size:
947 693 377 253 142 12
SanDI GG'GwC_CC
Cuts at: 0 265 2424
Size: 265 2159
ScaI AGT'ACT
Cuts at: 0 1358 2424
Size: 1358 1066
SexAI A'CCwGG_T
Cuts at: 0 1520 2424
Size: 1520 904
SfcI C'TryA_G
Cuts at: 0 98 616 1090 1374 1894 2424
Size: 98 518 474 284 520 530
Fragments arranged by size:
530 520 518 474 284 98
SfoI GGC'GCC
Cuts at: 0 1321 2424
Size: 1321 1103
SmaI CCC'GGG
Cuts at: 0 502 2424
Size: 502 1922
SmlI C'TyrA_G
Cuts at: 0 290 796 1252 2424
Size: 290 506 456 1172
Fragments arranged by size:
1172 506 456 290
SpeI A'CTAG_T
Cuts at: 0 1023 2424
Size: 1023 1401
StuI AGG'CCT
Cuts at: 0 1921 2424
Size: 1921 503
StyI C'CwwG_G
Cuts at: 0 1124 1331 1397 1865 2147 2232 2243 2318
Size: 1124 207 66 468 282 85 11 75
Cuts at: 2318 2424
Size: 106
Fragments arranged by size:
1124 468 282 207 106 85 75 66
11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 1367 1554 2424
Size: 556 811 187 870
Fragments arranged by size:
870 811 556 187
TatI w'GTAC_w
Cuts at: 0 1356 2424
Size: 1356 1068
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 2328 2360 2424
Size: 1013 32 1283 32 64
Fragments arranged by size:
1283 1013 64 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1489 2424
Size: 1489 935
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 162 1714 1936 2424
Size: 162 1552 222 488
Fragments arranged by size:
1552 488 222 162
XhoI C'TCGA_G
Cuts at: 0 290 2424
Size: 290 2134
XmaI C'CCGG_G
Cuts at: 0 500 2424
Size: 500 1924
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstXI
BstYI Bsu36I BtgI BtsI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII
HpaI KasI MmeI MscI MslI MspA1I NarI NspI
PasI PciI PflMI PfoI PpuMI PshAI PspXI PstI
PvuII SanDI ScaI SexAI SfcI SfoI SmaI SmlI
SpeI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BciVI BclI BfrBI BglII BmgBI BmtI BsaAI BsaBI
BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoRV FseI FspI FspAI KpnI MfeI
MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PsiI PspOMI PsrI
PvuI RsrII SacI SacII SalI SapI SbfI SfiI
SgfI SgrAI SnaBI SphI SrfI SspI SwaI XbaI
XmnI ZraI