Restriction map for
pFN21AE3429
(Linear) (Six-base) MAPSORT of: KIEE3429.seq Check: 9771 from: 1 to: 1965
KIEE3429.orf
With 182 enzymes: SgfI *
March 22, 2012 16:33 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 1547 1965
Size: 403 1144 418
Fragments arranged by size:
1144 418 403
AhdI GACnn_n'nnGTC
Cuts at: 0 786 1965
Size: 786 1179
AleI CACnn'nnGTG
Cuts at: 0 1273 1965
Size: 1273 692
ApaI G_GGCC'C
Cuts at: 0 892 1965
Size: 892 1073
ApaLI G'TGCA_C
Cuts at: 0 1510 1965
Size: 1510 455
ApoI r'AATT_y
Cuts at: 0 310 1167 1428 1765 1965
Size: 310 857 261 337 200
Fragments arranged by size:
857 337 310 261 200
AseI AT'TA_AT
Cuts at: 0 1061 1088 1965
Size: 1061 27 877
Fragments arranged by size:
1061 877 27
AvaI C'yCGr_G
Cuts at: 0 1679 1965
Size: 1679 286
BanI G'GyrC_C
Cuts at: 0 1725 1946 1965
Size: 1725 221 19
Fragments arranged by size:
1725 221 19
BanII G_rGCy'C
Cuts at: 0 729 892 1965
Size: 729 163 1073
Fragments arranged by size:
1073 729 163
BbsI GAAGACnn'nnnn_
Cuts at: 0 1277 1965
Size: 1277 688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 664 698 1965
Size: 664 34 1267
Fragments arranged by size:
1267 664 34
BclI T'GATC_A
Cuts at: 0 1703 1965
Size: 1703 262
BfrBI ATG'CAT
Cuts at: 0 1616 1965
Size: 1616 349
BglII A'GATC_T
Cuts at: 0 219 1965
Size: 219 1746
Bme1580I G_kGCm'C
Cuts at: 0 892 1514 1965
Size: 892 622 451
Fragments arranged by size:
892 622 451
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 743 1965
Size: 7 736 1222
Fragments arranged by size:
1222 736 7
Bpu10I CC'TnA_GC
Cuts at: 0 1192 1965
Size: 1192 773
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 1208 1456 1468 1965
Size: 502 706 248 12 497
Fragments arranged by size:
706 502 497 248 12
BsaBI GATnn'nnATC
Cuts at: 0 1688 1965
Size: 1688 277
BsaWI w'CCGG_w
Cuts at: 0 374 739 1965
Size: 374 365 1226
Fragments arranged by size:
1226 374 365
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 868 898 1965
Size: 868 30 1067
Fragments arranged by size:
1067 868 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 719 1965
Size: 151 568 1246
Fragments arranged by size:
1246 568 151
BseYI C'CCAG_C
Cuts at: 0 260 764 1965
Size: 260 504 1201
Fragments arranged by size:
1201 504 260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1070 1965
Size: 1070 895
BsiHKAI G_wGCw'C
Cuts at: 0 729 1514 1609 1965
Size: 729 785 95 356
Fragments arranged by size:
785 729 356 95
BsmI GAATG_Cn'
Cuts at: 0 860 1343 1965
Size: 860 483 622
Fragments arranged by size:
860 622 483
Bsp1286I G_dGCh'C
Cuts at: 0 729 892 1514 1609 1965
Size: 729 163 622 95 356
Fragments arranged by size:
729 622 356 163 95
BspEI T'CCGG_A
Cuts at: 0 739 1965
Size: 739 1226
BsrBI CCG'CTC
Cuts at: 0 6 1965
Size: 6 1959
BsrDI GCAATG_nn'
Cuts at: 0 326 1074 1694 1965
Size: 326 748 620 271
Fragments arranged by size:
748 620 326 271
BsrGI T'GTAC_A
Cuts at: 0 1240 1965
Size: 1240 725
BstAPI GCAn_nnn'nTGC
Cuts at: 0 513 1613 1965
Size: 513 1100 352
Fragments arranged by size:
1100 513 352
BstEII G'GTnAC_C
Cuts at: 0 1783 1965
Size: 1783 182
BstYI r'GATC_y
Cuts at: 0 148 171 219 742 1134 1573 1965
Size: 148 23 48 523 392 439 392
Fragments arranged by size:
523 439 392 392 148 48 23
BtgI C'CryG_G
Cuts at: 0 1271 1418 1965
Size: 1271 147 547
Fragments arranged by size:
1271 547 147
BtsI GCAGTG_nn'
Cuts at: 0 553 1925 1965
Size: 553 1372 40
Fragments arranged by size:
1372 553 40
DraI TTT'AAA
Cuts at: 0 453 1965
Size: 453 1512
DrdI GACnn_nn'nnGTC
Cuts at: 0 1159 1965
Size: 1159 806
EaeI y'GGCC_r
Cuts at: 0 1008 1631 1965
Size: 1008 623 334
Fragments arranged by size:
1008 623 334
EarI CTCTTCn'nnn_
Cuts at: 0 128 986 1664 1856 1912 1965
Size: 128 858 678 192 56 53
Fragments arranged by size:
858 678 192 128 56 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 722 1965
Size: 722 1243
EcoICRI GAG'CTC
Cuts at: 0 727 1965
Size: 727 1238
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 403 743 1547 1965
Size: 7 396 340 804 418
Fragments arranged by size:
804 418 396 340 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1661 1825 1965
Size: 1661 164 140
Fragments arranged by size:
1661 164 140
EcoO109I rG'GnC_Cy
Cuts at: 0 889 1818 1965
Size: 889 929 147
Fragments arranged by size:
929 889 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1003 1965
Size: 1003 962
HaeII r_GCGC'y
Cuts at: 0 831 1965
Size: 831 1134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 734 766 772 804 868 900 1965
Size: 734 32 6 32 64 32 1065
Fragments arranged by size:
1065 734 64 32 32 32 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1880 1965
Size: 1880 85
MscI TGG'CCA
Cuts at: 0 1010 1633 1965
Size: 1010 623 332
Fragments arranged by size:
1010 623 332
MslI CAynn'nnrTG
Cuts at: 0 1273 1965
Size: 1273 692
MspA1I CmG'CkG
Cuts at: 0 778 1870 1965
Size: 778 1092 95
Fragments arranged by size:
1092 778 95
NcoI C'CATG_G
Cuts at: 0 1271 1965
Size: 1271 694
NsiI A_TGCA'T
Cuts at: 0 1618 1965
Size: 1618 347
NspI r_CATG'y
Cuts at: 0 1616 1965
Size: 1616 349
PasI CC'CwG_GG
Cuts at: 0 892 1965
Size: 892 1073
PspOMI G'GGCC_C
Cuts at: 0 888 1965
Size: 888 1077
PstI C_TGCA'G
Cuts at: 0 699 750 1965
Size: 699 51 1215
Fragments arranged by size:
1215 699 51
PvuII CAG'CTG
Cuts at: 0 778 1870 1965
Size: 778 1092 95
Fragments arranged by size:
1092 778 95
SacI G_AGCT'C
Cuts at: 0 729 1965
Size: 729 1236
ScaI AGT'ACT
Cuts at: 0 756 1965
Size: 756 1209
SfcI C'TryA_G
Cuts at: 0 695 746 1295 1965
Size: 695 51 549 670
Fragments arranged by size:
695 670 549 51
SmlI C'TyrA_G
Cuts at: 0 517 1223 1447 1471 1679 1965
Size: 517 706 224 24 208 286
Fragments arranged by size:
706 517 286 224 208 24
SphI G_CATG'C
Cuts at: 0 1616 1965
Size: 1616 349
SspI AAT'ATT
Cuts at: 0 1756 1965
Size: 1756 209
StyI C'CwwG_G
Cuts at: 0 44 1271 1491 1556 1760 1813 1951 1965
Size: 44 1227 220 65 204 53 138 14
Fragments arranged by size:
1227 220 204 138 65 53 44 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1497 1965
Size: 1497 468
TatI w'GTAC_w
Cuts at: 0 677 754 1240 1673 1965
Size: 677 77 486 433 292
Fragments arranged by size:
677 486 433 292 77
XhoI C'TCGA_G
Cuts at: 0 1679 1965
Size: 1679 286
XmnI GAAnn'nnTTC
Cuts at: 0 1343 1532 1755 1965
Size: 1343 189 223 210
Fragments arranged by size:
1343 223 210 189
Enzymes that do cut:
AcuI AhdI AleI ApaI ApaLI ApoI AseI AvaI
BanI BanII BbsI BcgI BclI BfrBI BglII Bme1580I
BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrDI BsrGI
BstAPI BstEII BstYI BtgI BtsI DraI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I MmeI MscI MslI MspA1I NcoI NsiI NspI
PasI PspOMI PstI PvuII SacI ScaI SfcI SmlI
SphI SspI StyI TaqII TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI AlwNI AscI AsiSI AvrII BaeI
BamHI BbeI BbvCI BciVI BglI BlpI BmgBI BmrI
BmtI BplI BsaI BsaAI BsaHI BsiEI BsiWI BsmBI
BspHI BspMI BsrFI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII EagI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TstI
Tth111I XbaI XcmI XmaI ZraI