Restriction map for
pFN21AE3425
(Linear) (Six-base) MAPSORT of: KIEE3425.seq Check: 9150 from: 1 to: 1626
KIEE3425.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 324 766 1035 1626
Size: 324 442 269 591
Fragments arranged by size:
591 442 324 269
AatII G_ACGT'C
Cuts at: 0 685 1626
Size: 685 941
AccI GT'mk_AC
Cuts at: 0 1160 1626
Size: 1160 466
AfeI AGC'GCT
Cuts at: 0 798 1626
Size: 798 828
AflIII A'CryG_T
Cuts at: 0 963 1142 1626
Size: 963 179 484
Fragments arranged by size:
963 484 179
AgeI A'CCGG_T
Cuts at: 0 126 1626
Size: 126 1500
AleI CACnn'nnGTG
Cuts at: 0 1061 1343 1626
Size: 1061 282 283
Fragments arranged by size:
1061 283 282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 643 1626
Size: 643 983
AlwNI CAG_nnn'CTG
Cuts at: 0 874 1050 1132 1626
Size: 874 176 82 494
Fragments arranged by size:
874 494 176 82
ApaI G_GGCC'C
Cuts at: 0 677 1090 1229 1626
Size: 677 413 139 397
Fragments arranged by size:
677 413 397 139
ApaLI G'TGCA_C
Cuts at: 0 294 1558 1626
Size: 294 1264 68
Fragments arranged by size:
1264 294 68
AscI GG'CGCG_CC
Cuts at: 0 1373 1626
Size: 1373 253
AvaI C'yCGr_G
Cuts at: 0 447 615 669 676 758 831 924 1626
Size: 447 168 54 7 82 73 93 702
Fragments arranged by size:
702 447 168 93 82 73 54 7
BanI G'GyrC_C
Cuts at: 0 903 1095 1264 1272 1345 1357 1449 1473
Size: 903 192 169 8 73 12 92 24
Cuts at: 1473 1626
Size: 153
Fragments arranged by size:
903 192 169 153 92 73 24 12
8
BanII G_rGCy'C
Cuts at: 0 556 677 838 856 1090 1229 1291 1626
Size: 556 121 161 18 234 139 62 335
Fragments arranged by size:
556 335 234 161 139 121 62 18
BbeI G_GCGC'C
Cuts at: 0 907 1099 1268 1276 1361 1453 1626
Size: 907 192 169 8 85 92 173
Fragments arranged by size:
907 192 173 169 92 85 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 459 1626
Size: 459 1167
BbvCI CC'TCA_GC
Cuts at: 0 1387 1626
Size: 1387 239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 285 319 1626
Size: 285 34 1307
Fragments arranged by size:
1307 285 34
BglI GCCn_nnn'nGGC
Cuts at: 0 109 617 809 851 901 1447 1626
Size: 109 508 192 42 50 546 179
Fragments arranged by size:
546 508 192 179 109 50 42
BlpI GC'TnA_GC
Cuts at: 0 1294 1626
Size: 1294 332
Bme1580I G_kGCm'C
Cuts at: 0 32 298 677 1090 1229 1478 1562 1626
Size: 32 266 379 413 139 249 84 64
Fragments arranged by size:
413 379 266 249 139 84 64 32
BmgBI CAC'GTC
Cuts at: 0 1026 1626
Size: 1026 600
BmtI G_CTAG'C
Cuts at: 0 845 1626
Size: 845 781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 1626
Size: 27 1599
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1015 1626
Size: 524 491 611
Fragments arranged by size:
611 524 491
Bpu10I CC'TnA_GC
Cuts at: 0 708 1387 1626
Size: 708 679 239
Fragments arranged by size:
708 679 239
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 1626
Size: 229 1397
BsaHI Gr'CG_yC
Cuts at: 0 79 88 682 904 943 989 1096 1163
Size: 79 9 594 222 39 46 107 67
Cuts at: 1163 1265 1273 1358 1450 1626
Size: 102 8 85 92 176
Fragments arranged by size:
594 222 176 107 102 92 85 79
67 46 39 9 8
BsaWI w'CCGG_w
Cuts at: 0 126 526 862 1614 1626
Size: 126 400 336 752 12
Fragments arranged by size:
752 400 336 126 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 988 1018 1626
Size: 988 30 608
Fragments arranged by size:
988 608 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 379 835 1626
Size: 379 456 791
Fragments arranged by size:
791 456 379
BseYI C'CCAG_C
Cuts at: 0 22 912 1150 1284 1368 1626
Size: 22 890 238 134 84 258
Fragments arranged by size:
890 258 238 134 84 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1051 1626
Size: 1051 575
BsiEI CG_ry'CG
Cuts at: 0 113 1079 1541 1626
Size: 113 966 462 85
Fragments arranged by size:
966 462 113 85
BsiHKAI G_wGCw'C
Cuts at: 0 298 1562 1626
Size: 298 1264 64
Fragments arranged by size:
1264 298 64
Bsp1286I G_dGCh'C
Cuts at: 0 32 298 556 677 838 856 1090 1229
Size: 32 266 258 121 161 18 234 139
Cuts at: 1229 1291 1478 1562 1626
Size: 62 187 84 64
Fragments arranged by size:
266 258 234 187 161 139 121 84
64 62 32 18
BspEI T'CCGG_A
Cuts at: 0 1614 1626
Size: 1614 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 324 766 1035 1626
Size: 324 442 269 591
Fragments arranged by size:
591 442 324 269
BsrBI CCG'CTC
Cuts at: 0 508 1304 1380 1535 1626
Size: 508 796 76 155 91
Fragments arranged by size:
796 508 155 91 76
BsrFI r'CCGG_y
Cuts at: 0 126 636 900 980 1078 1261 1465 1626
Size: 126 510 264 80 98 183 204 161
Fragments arranged by size:
510 264 204 183 161 126 98 80
BssHII G'CGCG_C
Cuts at: 0 209 211 376 1373 1626
Size: 209 2 165 997 253
Fragments arranged by size:
997 253 209 165 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1132 1626
Size: 1132 494
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1063 1626
Size: 1063 563
BtgI C'CryG_G
Cuts at: 0 104 193 225 518 931 1062 1115 1268
Size: 104 89 32 293 413 131 53 153
Cuts at: 1268 1412 1431 1499 1626
Size: 144 19 68 127
Fragments arranged by size:
413 293 153 144 131 127 104 89
68 53 32 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 104 288 740 1626
Size: 104 184 452 886
Fragments arranged by size:
886 452 184 104
BtsI GCAGTG_nn'
Cuts at: 0 401 1460 1626
Size: 401 1059 166
Fragments arranged by size:
1059 401 166
EaeI y'GGCC_r
Cuts at: 0 110 422 1076 1259 1538 1626
Size: 110 312 654 183 279 88
Fragments arranged by size:
654 312 279 183 110 88
EagI C'GGCC_G
Cuts at: 0 110 1076 1538 1626
Size: 110 966 462 88
Fragments arranged by size:
966 462 110 88
EarI CTCTTCn'nnn_
Cuts at: 0 878 1626
Size: 878 748
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 548 932 1480 1626
Size: 548 384 548 146
Fragments arranged by size:
548 548 384 146
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 524 1015 1626
Size: 524 491 611
Fragments arranged by size:
611 524 491
EcoO109I rG'GnC_Cy
Cuts at: 0 499 673 1068 1087 1195 1225 1626
Size: 499 174 395 19 108 30 401
Fragments arranged by size:
499 401 395 174 108 30 19
EcoRV GAT'ATC
Cuts at: 0 954 1626
Size: 954 672
FseI GG_CCGG'CC
Cuts at: 0 1082 1626
Size: 1082 544
FspI TGC'GCA
Cuts at: 0 346 1626
Size: 346 1280
HaeII r_GCGC'y
Cuts at: 0 315 343 421 517 800 907 1099 1268
Size: 315 28 78 96 283 107 192 169
Cuts at: 1268 1276 1285 1361 1453 1613 1626
Size: 8 9 76 92 160 13
Fragments arranged by size:
315 283 192 169 160 107 96 92
78 76 28 13 9 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 718 750 1626
Size: 718 32 876
Fragments arranged by size:
876 718 32
HincII GTy'rAC
Cuts at: 0 1161 1605 1626
Size: 1161 444 21
Fragments arranged by size:
1161 444 21
KasI G'GCGC_C
Cuts at: 0 903 1095 1264 1272 1357 1449 1626
Size: 903 192 169 8 85 92 177
Fragments arranged by size:
903 192 177 169 92 85 8
MluI A'CGCG_T
Cuts at: 0 963 1142 1626
Size: 963 179 484
Fragments arranged by size:
963 484 179
MslI CAynn'nnrTG
Cuts at: 0 1061 1343 1626
Size: 1061 282 283
Fragments arranged by size:
1061 283 282
MspA1I CmG'CkG
Cuts at: 0 106 115 195 390 520 541 627 785
Size: 106 9 80 195 130 21 86 158
Cuts at: 785 939 948 1132 1186 1215 1320 1368 1414
Size: 154 9 184 54 29 105 48 46
Cuts at: 1414 1433 1546 1626
Size: 19 113 80
Fragments arranged by size:
195 184 158 154 130 113 106 105
86 80 80 54 48 46 29 21
19 9 9
NaeI GCC'GGC
Cuts at: 0 902 1080 1263 1467 1626
Size: 902 178 183 204 159
Fragments arranged by size:
902 204 183 178 159
NarI GG'CG_CC
Cuts at: 0 904 1096 1265 1273 1358 1450 1626
Size: 904 192 169 8 85 92 176
Fragments arranged by size:
904 192 176 169 92 85 8
NcoI C'CATG_G
Cuts at: 0 225 1268 1499 1626
Size: 225 1043 231 127
Fragments arranged by size:
1043 231 225 127
NgoMIV G'CCGG_C
Cuts at: 0 900 1078 1261 1465 1626
Size: 900 178 183 204 161
Fragments arranged by size:
900 204 183 178 161
NheI G'CTAG_C
Cuts at: 0 841 1626
Size: 841 785
NotI GC'GGCC_GC
Cuts at: 0 110 1626
Size: 110 1516
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 1626
Size: 231 1395
PfoI T'CCnGG_A
Cuts at: 0 992 1626
Size: 992 634
PpuMI rG'GwC_Cy
Cuts at: 0 499 1626
Size: 499 1127
PspOMI G'GGCC_C
Cuts at: 0 673 1086 1225 1626
Size: 673 413 139 401
Fragments arranged by size:
673 413 401 139
PstI C_TGCA'G
Cuts at: 0 389 605 1128 1137 1220 1325 1626
Size: 389 216 523 9 83 105 301
Fragments arranged by size:
523 389 301 216 105 83 9
PvuII CAG'CTG
Cuts at: 0 390 541 785 1132 1626
Size: 390 151 244 347 494
Fragments arranged by size:
494 390 347 244 151
SacII CC_GC'GG
Cuts at: 0 107 196 521 1415 1434 1626
Size: 107 89 325 894 19 192
Fragments arranged by size:
894 325 192 107 89 19
SalI G'TCGA_C
Cuts at: 0 1159 1626
Size: 1159 467
SfcI C'TryA_G
Cuts at: 0 385 601 1124 1133 1216 1321 1626
Size: 385 216 523 9 83 105 305
Fragments arranged by size:
523 385 305 216 105 83 9
SfoI GGC'GCC
Cuts at: 0 905 1097 1266 1274 1359 1451 1626
Size: 905 192 169 8 85 92 175
Fragments arranged by size:
905 192 175 169 92 85 8
SgrAI Cr'CCGG_yG
Cuts at: 0 636 900 980 1626
Size: 636 264 80 646
Fragments arranged by size:
646 636 264 80
SmaI CCC'GGG
Cuts at: 0 617 678 833 1626
Size: 617 61 155 793
Fragments arranged by size:
793 617 155 61
SmlI C'TyrA_G
Cuts at: 0 244 1626
Size: 244 1382
SrfI GCCC'GGGC
Cuts at: 0 617 833 1626
Size: 617 216 793
Fragments arranged by size:
793 617 216
StyI C'CwwG_G
Cuts at: 0 225 1268 1499 1626
Size: 225 1043 231 127
Fragments arranged by size:
1043 231 225 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1108 1512 1626
Size: 1108 404 114
Fragments arranged by size:
1108 404 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 268 300 953 985 1626
Size: 268 32 653 32 641
Fragments arranged by size:
653 641 268 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 684 1626
Size: 684 942
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1467 1626
Size: 1467 159
XmaI C'CCGG_G
Cuts at: 0 615 676 831 1626
Size: 615 61 155 795
Fragments arranged by size:
795 615 155 61
ZraI GAC'GTC
Cuts at: 0 683 1626
Size: 683 943
Enzymes that do cut:
AarI AatII AccI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI AscI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BstAPI BstXI BtgI BtgZI BtsI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRV FseI FspI
HaeII Hin4I HincII KasI MluI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI PflMI PfoI PpuMI
PspOMI PstI PvuII SacII SalI SfcI SfoI SgrAI
SmaI SmlI SrfI StyI TaqII TstI Tth111I XcmI
XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AflII AhdI AloI ApoI AseI
AsiSI AvrII BaeI BamHI BciVI BclI BfrBI BglII
BmrI BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EcoICRI EcoNI EcoRI
FalI FspAI HindIII HpaI KpnI MfeI MmeI MscI
NdeI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SspI StuI SwaI TatI XbaI
XhoI XmnI