(Linear) (Six-base) MAPSORT of: KIEE3420.seq  Check: 6876  from: 1  to: 1449



KIEE3420.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:50  ..

AclI AA'CG_TT

Cuts at:      0     741    1449 
   Size:        741     708
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584    1424    1449 
   Size:        584     840      25

  Fragments arranged by size: 

                 840     584      25
 
AflIII A'CryG_T

Cuts at:      0    1216    1449 
   Size:       1216     233
 
ApoI r'AATT_y

Cuts at:      0     135     275     282     402     470     554     653     890 
   Size:        135     140       7     120      68      84      99     237

Cuts at:    890     974    1226    1310    1449 
   Size:         84     252      84     139

  Fragments arranged by size: 

                 252     237     140     139     135     120      99      84
                  84      84      68       7
 
BbvCI CC'TCA_GC

Cuts at:      0    1119    1449 
   Size:       1119     330
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     656    1449 
   Size:        656     793
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1261    1449 
   Size:       1261     188
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     500     836    1004    1088    1172    1256    1340    1449 
   Size:        500     336     168      84      84      84      84     109

  Fragments arranged by size: 

                 500     336     168     109      84      84      84      84
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1119    1449 
   Size:       1119     330
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249    1449 
   Size:        249    1200
 
BsaWI w'CCGG_w

Cuts at:      0     730    1449 
   Size:        730     719
 
BsmI GAATG_Cn'

Cuts at:      0     763    1449 
   Size:        763     686
 
BsrGI T'GTAC_A

Cuts at:      0     827    1449 
   Size:        827     622
 
DraI TTT'AAA

Cuts at:      0     327     942    1449 
   Size:        327     615     507

  Fragments arranged by size: 

                 615     507     327
 
EaeI y'GGCC_r

Cuts at:      0      64    1449 
   Size:         64    1385
 
EarI CTCTTCn'nnn_

Cuts at:      0     423     560    1400    1449 
   Size:        423     137     840      49

  Fragments arranged by size: 

                 840     423     137      49
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     500     584     836    1004    1088    1172    1256    1340 
   Size:        500      84     252     168      84      84      84      84

Cuts at:   1340    1424    1449 
   Size:         84      25

  Fragments arranged by size: 

                 500     252     168      84      84      84      84      84
                  84      25
 
EcoRI G'AATT_C

Cuts at:      0     470     554     890    1310    1449 
   Size:        470      84     336     420     139

  Fragments arranged by size: 

                 470     420     336     139      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     104    1449 
   Size:        104    1345
 
HindIII A'AGCT_T

Cuts at:      0     703     767     935    1019    1355    1439    1449 
   Size:        703      64     168      84     336      84      10

  Fragments arranged by size: 

                 703     336     168      84      84      64      10
 
MscI TGG'CCA

Cuts at:      0      66    1449 
   Size:         66    1383
 
NdeI CA'TA_TG

Cuts at:      0     423    1449 
   Size:        423    1026
 
NspI r_CATG'y

Cuts at:      0     360    1220    1449 
   Size:        360     860     229

  Fragments arranged by size: 

                 860     360     229
 
PciI A'CATG_T

Cuts at:      0    1216    1449 
   Size:       1216     233
 
SapI GCTCTTCn'nnn_

Cuts at:      0     423    1449 
   Size:        423    1026
 
SmlI C'TyrA_G

Cuts at:      0     228    1449 
   Size:        228    1221
 
SphI G_CATG'C

Cuts at:      0     360    1449 
   Size:        360    1089
 
SspI AAT'ATT

Cuts at:      0     239    1449 
   Size:        239    1210
 
TatI w'GTAC_w

Cuts at:      0     803     827    1213    1415    1449 
   Size:        803      24     386     202      34

  Fragments arranged by size: 

                 803     386     202      34      24
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     ApoI    BbvCI     BmrI     BplI     BpmI
   Bpu10I    BpuEI    BsaWI     BsmI    BsrGI     DraI     EaeI     EarI
  Eco57MI    EcoRI     FalI  HindIII     MscI     NdeI     NspI     PciI
     SapI     SmlI     SphI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI