(Linear) (Six-base) MAPSORT of: KIEE3414.seq  Check: 7821  from: 1  to: 1059



KIEE3414.orf

 With 182 enzymes: SgfI * 

                        October 20, 2017 17:04  ..

AflII C'TTAA_G

Cuts at:      0      81    1059 
   Size:         81     978
 
ApoI r'AATT_y

Cuts at:      0     651    1059 
   Size:        651     408
 
BamHI G'GATC_C

Cuts at:      0     162    1059 
   Size:        162     897
 
BanII G_rGCy'C

Cuts at:      0     321    1059 
   Size:        321     738
 
BbvCI CC'TCA_GC

Cuts at:      0     218    1059 
   Size:        218     841
 
BmgBI CAC'GTC

Cuts at:      0     712    1059 
   Size:        712     347
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773    1059 
   Size:        773     286
 
Bpu10I CC'TnA_GC

Cuts at:      0     218    1059 
   Size:        218     841
 
BsaAI yAC'GTr

Cuts at:      0     637    1059 
   Size:        637     422
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     623     698     758     803     812     887    1059 
   Size:        623      75      60      45       9      75     172

  Fragments arranged by size: 

                 623     172      75      75      60      45       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     321    1059 
   Size:        321     738
 
BstYI r'GATC_y

Cuts at:      0     162     688    1059 
   Size:        162     526     371

  Fragments arranged by size: 

                 526     371     162
 
BtgI C'CryG_G

Cuts at:      0     212     233    1059 
   Size:        212      21     826

  Fragments arranged by size: 

                 826     212      21
 
BtsI GCAGTG_nn'

Cuts at:      0     711    1026    1059 
   Size:        711     315      33

  Fragments arranged by size: 

                 711     315      33
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     819     854    1059 
   Size:        819      35     205

  Fragments arranged by size: 

                 819     205      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     640    1059 
   Size:        640     419
 
EarI CTCTTCn'nnn_

Cuts at:      0     600     789     943    1059 
   Size:        600     189     154     116

  Fragments arranged by size: 

                 600     189     154     116
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     365    1059 
   Size:        365     694
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     773    1059 
   Size:        773     286
 
EcoO109I rG'GnC_Cy

Cuts at:      0     748     817    1059 
   Size:        748      69     242

  Fragments arranged by size: 

                 748     242      69
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     575    1059 
   Size:        575     484
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     427     459    1059 
   Size:        427      32     600

  Fragments arranged by size: 

                 600     427      32
 
HindIII A'AGCT_T

Cuts at:      0      84     136    1059 
   Size:         84      52     923

  Fragments arranged by size: 

                 923      84      52
 
MfeI C'AATT_G

Cuts at:      0     254    1059 
   Size:        254     805
 
NcoI C'CATG_G

Cuts at:      0     212     233    1059 
   Size:        212      21     826

  Fragments arranged by size: 

                 826     212      21
 
NdeI CA'TA_TG

Cuts at:      0     990    1059 
   Size:        990      69
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     994    1059 
   Size:        994      65
 
PfoI T'CCnGG_A

Cuts at:      0    1011    1059 
   Size:       1011      48
 
PmlI CAC'GTG

Cuts at:      0     637    1059 
   Size:        637     422
 
PpuMI rG'GwC_Cy

Cuts at:      0     748     817    1059 
   Size:        748      69     242

  Fragments arranged by size: 

                 748     242      69
 
PsiI TTA'TAA

Cuts at:      0     917    1059 
   Size:        917     142
 
SfcI C'TryA_G

Cuts at:      0     287    1059 
   Size:        287     772
 
SmlI C'TyrA_G

Cuts at:      0      81    1059 
   Size:         81     978
 
StyI C'CwwG_G

Cuts at:      0     212     233    1059 
   Size:        212      21     826

  Fragments arranged by size: 

                 826     212      21
 
XbaI T'CTAG_A

Cuts at:      0     565    1059 
   Size:        565     494
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     529    1059 
   Size:        529     530
 
XmnI GAAnn'nnTTC

Cuts at:      0      20    1059 
   Size:         20    1039
 

 Enzymes that do cut:

    AflII     ApoI    BamHI    BanII    BbvCI    BmgBI     BpmI   Bpu10I
    BsaAI    BseRI Bsp1286I    BstYI     BtgI     BtsI    CspCI   DraIII
     EarI     EciI  Eco57MI EcoO109I     FalI    Hin4I  HindIII     MfeI
     NcoI     NdeI    PflMI     PfoI     PmlI    PpuMI     PsiI     SfcI
     SmlI     StyI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI     DraI     DrdI     EaeI     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI     TstI  Tth111I     XhoI     XmaI     ZraI