Restriction map for
pFN21AE3411
(Linear) (Six-base) MAPSORT of: KIEE3411.seq Check: 7831 from: 1 to: 945
KIEE3411.orf
With 182 enzymes: SgfI *
March 22, 2012 16:32 ..
AccI GT'mk_AC
Cuts at: 0 714 945
Size: 714 231
Acc65I G'GTAC_C
Cuts at: 0 494 945
Size: 494 451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 524 820 945
Size: 134 390 296 125
Fragments arranged by size:
390 296 134 125
AlwNI CAG_nnn'CTG
Cuts at: 0 597 945
Size: 597 348
ApoI r'AATT_y
Cuts at: 0 100 446 945
Size: 100 346 499
Fragments arranged by size:
499 346 100
AseI AT'TA_AT
Cuts at: 0 658 945
Size: 658 287
BanI G'GyrC_C
Cuts at: 0 494 945
Size: 494 451
BanII G_rGCy'C
Cuts at: 0 504 581 850 945
Size: 504 77 269 95
Fragments arranged by size:
504 269 95 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 734 921 945
Size: 734 187 24
Fragments arranged by size:
734 187 24
BclI T'GATC_A
Cuts at: 0 249 305 945
Size: 249 56 640
Fragments arranged by size:
640 249 56
BfrBI ATG'CAT
Cuts at: 0 228 945
Size: 228 717
BglI GCCn_nnn'nGGC
Cuts at: 0 600 945
Size: 600 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 862 945
Size: 50 812 83
Fragments arranged by size:
812 83 50
Bpu10I CC'TnA_GC
Cuts at: 0 719 945
Size: 719 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 945
Size: 763 182
BsaAI yAC'GTr
Cuts at: 0 157 895 945
Size: 157 738 50
Fragments arranged by size:
738 157 50
BsaBI GATnn'nnATC
Cuts at: 0 764 945
Size: 764 181
BsaHI Gr'CG_yC
Cuts at: 0 918 945
Size: 918 27
BsiHKAI G_wGCw'C
Cuts at: 0 504 581 850 945
Size: 504 77 269 95
Fragments arranged by size:
504 269 95 77
Bsp1286I G_dGCh'C
Cuts at: 0 504 581 850 945
Size: 504 77 269 95
Fragments arranged by size:
504 269 95 77
BspHI T'CATG_A
Cuts at: 0 785 945
Size: 785 160
BsrBI CCG'CTC
Cuts at: 0 936 945
Size: 936 9
BstZ17I GTA'TAC
Cuts at: 0 715 945
Size: 715 230
BtgI C'CryG_G
Cuts at: 0 702 945
Size: 702 243
DraI TTT'AAA
Cuts at: 0 263 684 945
Size: 263 421 261
Fragments arranged by size:
421 263 261
EcoICRI GAG'CTC
Cuts at: 0 502 579 848 945
Size: 502 77 269 97
Fragments arranged by size:
502 269 97 77
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 134 524 820 862 945
Size: 50 84 390 296 42 83
Fragments arranged by size:
390 296 84 83 50 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 199 945
Size: 199 746
KpnI G_GTAC'C
Cuts at: 0 498 945
Size: 498 447
MslI CAynn'nnrTG
Cuts at: 0 202 945
Size: 202 743
NcoI C'CATG_G
Cuts at: 0 702 945
Size: 702 243
NsiI A_TGCA'T
Cuts at: 0 230 945
Size: 230 715
PflMI CCAn_nnn'nTGG
Cuts at: 0 840 945
Size: 840 105
PfoI T'CCnGG_A
Cuts at: 0 456 839 945
Size: 456 383 106
Fragments arranged by size:
456 383 106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 945
Size: 736 32 177
Fragments arranged by size:
736 177 32
SacI G_AGCT'C
Cuts at: 0 504 581 850 945
Size: 504 77 269 95
Fragments arranged by size:
504 269 95 77
SmlI C'TyrA_G
Cuts at: 0 742 945
Size: 742 203
SnaBI TAC'GTA
Cuts at: 0 157 945
Size: 157 788
SspI AAT'ATT
Cuts at: 0 393 945
Size: 393 552
StyI C'CwwG_G
Cuts at: 0 702 945
Size: 702 243
XmnI GAAnn'nnTTC
Cuts at: 0 392 945
Size: 392 553
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApoI AseI BanI BanII
BbsI BclI BfrBI BglI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsiHKAI Bsp1286I BspHI BsrBI BstZ17I BtgI
DraI EcoICRI Eco57MI EcoNI KpnI MslI NcoI NsiI
PflMI PfoI PpiI SacI SmlI SnaBI SspI StyI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BsaI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgZI BtsI ClaI
CspCI DraIII DrdI EaeI EagI EarI EciI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI MfeI MluI MmeI MscI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI