Restriction map for
pFN21AE3410
(Linear) (Six-base) MAPSORT of: KIEE3410.seq Check: 5610 from: 1 to: 930
KIEE3410.orf
With 182 enzymes: SgfI *
March 22, 2012 16:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 102 930
Size: 102 828
AatII G_ACGT'C
Cuts at: 0 623 930
Size: 623 307
AgeI A'CCGG_T
Cuts at: 0 888 930
Size: 888 42
AhdI GACnn_n'nnGTC
Cuts at: 0 602 930
Size: 602 328
AlwNI CAG_nnn'CTG
Cuts at: 0 522 930
Size: 522 408
BanII G_rGCy'C
Cuts at: 0 127 503 930
Size: 127 376 427
Fragments arranged by size:
427 376 127
BglI GCCn_nnn'nGGC
Cuts at: 0 10 930
Size: 10 920
Bme1580I G_kGCm'C
Cuts at: 0 567 930
Size: 567 363
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 825 930
Size: 825 105
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 809 930
Size: 715 94 121
Fragments arranged by size:
715 121 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 344 930
Size: 344 586
BsaBI GATnn'nnATC
Cuts at: 0 83 352 930
Size: 83 269 578
Fragments arranged by size:
578 269 83
BsaHI Gr'CG_yC
Cuts at: 0 620 930
Size: 620 310
BsaWI w'CCGG_w
Cuts at: 0 37 366 888 930
Size: 37 329 522 42
Fragments arranged by size:
522 329 42 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 348 378 930
Size: 348 30 552
Fragments arranged by size:
552 348 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 353 871 930
Size: 114 239 518 59
Fragments arranged by size:
518 239 114 59
BseYI C'CCAG_C
Cuts at: 0 22 257 439 930
Size: 22 235 182 491
Fragments arranged by size:
491 235 182 22
BsiEI CG_ry'CG
Cuts at: 0 889 930
Size: 889 41
BsiHKAI G_wGCw'C
Cuts at: 0 127 245 930
Size: 127 118 685
Fragments arranged by size:
685 127 118
Bsp1286I G_dGCh'C
Cuts at: 0 127 245 503 567 930
Size: 127 118 258 64 363
Fragments arranged by size:
363 258 127 118 64
BspEI T'CCGG_A
Cuts at: 0 37 930
Size: 37 893
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 102 930
Size: 102 828
BsrBI CCG'CTC
Cuts at: 0 561 930
Size: 561 369
BsrFI r'CCGG_y
Cuts at: 0 28 888 930
Size: 28 860 42
Fragments arranged by size:
860 42 28
BstEII G'GTnAC_C
Cuts at: 0 369 930
Size: 369 561
BstYI r'GATC_y
Cuts at: 0 905 930
Size: 905 25
BtgI C'CryG_G
Cuts at: 0 170 930
Size: 170 760
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 566 930
Size: 566 364
BtsI GCAGTG_nn'
Cuts at: 0 356 401 930
Size: 356 45 529
Fragments arranged by size:
529 356 45
ClaI AT'CG_AT
Cuts at: 0 869 930
Size: 869 61
DrdI GACnn_nn'nnGTC
Cuts at: 0 517 930
Size: 517 413
EaeI y'GGCC_r
Cuts at: 0 2 14 581 930
Size: 2 12 567 349
Fragments arranged by size:
567 349 12 2
EarI CTCTTCn'nnn_
Cuts at: 0 683 707 770 930
Size: 683 24 63 160
Fragments arranged by size:
683 160 63 24
EcoICRI GAG'CTC
Cuts at: 0 125 930
Size: 125 805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 809 930
Size: 715 94 121
Fragments arranged by size:
715 121 94
EcoO109I rG'GnC_Cy
Cuts at: 0 254 396 519 930
Size: 254 142 123 411
Fragments arranged by size:
411 254 142 123
EcoRV GAT'ATC
Cuts at: 0 276 930
Size: 276 654
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 490 522 588 620 930
Size: 151 32 307 32 66 32 310
Fragments arranged by size:
310 307 151 66 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 930
Size: 511 419
MscI TGG'CCA
Cuts at: 0 583 930
Size: 583 347
MspA1I CmG'CkG
Cuts at: 0 22 930
Size: 22 908
PpuMI rG'GwC_Cy
Cuts at: 0 254 519 930
Size: 254 265 411
Fragments arranged by size:
411 265 254
PstI C_TGCA'G
Cuts at: 0 113 197 408 821 930
Size: 113 84 211 413 109
Fragments arranged by size:
413 211 113 109 84
PvuII CAG'CTG
Cuts at: 0 22 930
Size: 22 908
SacI G_AGCT'C
Cuts at: 0 127 930
Size: 127 803
SapI GCTCTTCn'nnn_
Cuts at: 0 683 770 930
Size: 683 87 160
Fragments arranged by size:
683 160 87
SbfI CC_TGCA'GG
Cuts at: 0 113 930
Size: 113 817
SexAI A'CCwGG_T
Cuts at: 0 515 930
Size: 515 415
SfcI C'TryA_G
Cuts at: 0 109 193 404 817 930
Size: 109 84 211 413 113
Fragments arranged by size:
413 211 113 109 84
StyI C'CwwG_G
Cuts at: 0 392 930
Size: 392 538
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 348 380 930
Size: 348 32 550
Fragments arranged by size:
550 348 32
ZraI GAC'GTC
Cuts at: 0 621 930
Size: 621 309
Enzymes that do cut:
AarI AatII AgeI AhdI AlwNI BanII BglI Bme1580I
BplI BpmI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI
BstEII BstYI BtgI BtgZI BtsI ClaI DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I EcoRV Hin4I MmeI MscI
MspA1I PpuMI PstI PvuII SacI SapI SbfI SexAI
SfcI StyI TstI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI
BmtI Bpu10I BpuEI BsaAI BsgI BsiWI BsmI BsmBI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I Bsu36I CspCI DraI DraIII EagI EciI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI