(Linear) (Six-base) MAPSORT of: KIEE3408.seq  Check: 9847  from: 1  to: 879



KIEE3408.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 16:32  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     764     879 
   Size:        764     115
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60     879 
   Size:         60     819
 
ApaLI G'TGCA_C

Cuts at:      0     680     879 
   Size:        680     199
 
ApoI r'AATT_y

Cuts at:      0     231     370     378     482     498     566     879 
   Size:        231     139       8     104      16      68     313

  Fragments arranged by size: 

                 313     231     139     104      68      16       8
 
BfrBI ATG'CAT

Cuts at:      0     366     879 
   Size:        366     513
 
Bme1580I G_kGCm'C

Cuts at:      0     684     879 
   Size:        684     195
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596     680     848     879 
   Size:        596      84     168      31

  Fragments arranged by size: 

                 596     168      84      31
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      40      70     879 
   Size:         40      30     809

  Fragments arranged by size: 

                 809      40      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     879 
   Size:         61     818
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      53     879 
   Size:         53     826
 
BsiHKAI G_wGCw'C

Cuts at:      0     684     879 
   Size:        684     195
 
Bsp1286I G_dGCh'C

Cuts at:      0     684     879 
   Size:        684     195
 
BtsI GCAGTG_nn'

Cuts at:      0      61      65     879 
   Size:         61       4     814

  Fragments arranged by size: 

                 814      61       4
 
DraI TTT'AAA

Cuts at:      0     702     879 
   Size:        702     177
 
DraIII CAC_nnn'GTG

Cuts at:      0     688     879 
   Size:        688     191
 
EaeI y'GGCC_r

Cuts at:      0      26     879 
   Size:         26     853
 
EarI CTCTTCn'nnn_

Cuts at:      0     509     740     879 
   Size:        509     231     139

  Fragments arranged by size: 

                 509     231     139
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     596     680     764     848     879 
   Size:        596      84      84      84      31

  Fragments arranged by size: 

                 596      84      84      84      31
 
HindIII A'AGCT_T

Cuts at:      0     863     879 
   Size:        863      16
 
MscI TGG'CCA

Cuts at:      0      28     879 
   Size:         28     851
 
MslI CAynn'nnrTG

Cuts at:      0     843     879 
   Size:        843      36
 
MspA1I CmG'CkG

Cuts at:      0      75     879 
   Size:         75     804
 
NsiI A_TGCA'T

Cuts at:      0     368     879 
   Size:        368     511
 
NspI r_CATG'y

Cuts at:      0      94     456     879 
   Size:         94     362     423

  Fragments arranged by size: 

                 423     362      94
 
PsiI TTA'TAA

Cuts at:      0     293     879 
   Size:        293     586
 
SexAI A'CCwGG_T

Cuts at:      0     116     879 
   Size:        116     763
 
SfcI C'TryA_G

Cuts at:      0     108     879 
   Size:        108     771
 
SphI G_CATG'C

Cuts at:      0     456     879 
   Size:        456     423
 
SspI AAT'ATT

Cuts at:      0     335     879 
   Size:        335     544
 
StyI C'CwwG_G

Cuts at:      0     282     879 
   Size:        282     597
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      40      72     879 
   Size:         40      32     807

  Fragments arranged by size: 

                 807      40      32
 

 Enzymes that do cut:

     AcuI    AlwNI    ApaLI     ApoI    BfrBI Bme1580I     BpmI    BsaXI
    BseRI     BsgI  BsiHKAI Bsp1286I     BtsI     DraI   DraIII     EaeI
     EarI  Eco57MI  HindIII     MscI     MslI   MspA1I     NsiI     NspI
     PsiI    SexAI     SfcI     SphI     SspI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI