(Linear) (Six-base) MAPSORT of: KIEE3407.seq  Check: 3894  from: 1  to: 852



KIEE3407.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 17:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     769     852 
   Size:         68     701      83

  Fragments arranged by size: 

                 701      83      68
 
AlwNI CAG_nnn'CTG

Cuts at:      0     182     448     852 
   Size:        182     266     404

  Fragments arranged by size: 

                 404     266     182
 
ApoI r'AATT_y

Cuts at:      0     793     852 
   Size:        793      59
 
BamHI G'GATC_C

Cuts at:      0     840     852 
   Size:        840      12
 
BclI T'GATC_A

Cuts at:      0     579     852 
   Size:        579     273
 
BlpI GC'TnA_GC

Cuts at:      0     683     852 
   Size:        683     169
 
Bpu10I CC'TnA_GC

Cuts at:      0     592     852 
   Size:        592     260
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      65     852 
   Size:         65     787
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70     217     852 
   Size:         70     147     635

  Fragments arranged by size: 

                 635     147      70
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     733     852 
   Size:        733     119
 
BstYI r'GATC_y

Cuts at:      0     135     300     840     852 
   Size:        135     165     540      12

  Fragments arranged by size: 

                 540     165     135      12
 
DraI TTT'AAA

Cuts at:      0      96     600     852 
   Size:         96     504     252

  Fragments arranged by size: 

                 504     252      96
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     852 
   Size:         47     805
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     769     852 
   Size:         68     701      83

  Fragments arranged by size: 

                 701      83      68
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     439     852 
   Size:        439     413
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     196     228     832     852 
   Size:        196      32     604      20

  Fragments arranged by size: 

                 604     196      32      20
 
HincII GTy'rAC

Cuts at:      0     242     852 
   Size:        242     610
 
HpaI GTT'AAC

Cuts at:      0     242     852 
   Size:        242     610
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     852 
   Size:        249     603
 
MspA1I CmG'CkG

Cuts at:      0     484     852 
   Size:        484     368
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      81     852 
   Size:         81     771
 
PstI C_TGCA'G

Cuts at:      0      29     852 
   Size:         29     823
 
PvuII CAG'CTG

Cuts at:      0     484     852 
   Size:        484     368
 
SfcI C'TryA_G

Cuts at:      0      25     852 
   Size:         25     827
 
StuI AGG'CCT

Cuts at:      0     761     852 
   Size:        761      91
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BamHI     BclI     BlpI   Bpu10I     BsaI
    BseRI    BsmBI    BstYI     DraI     EarI  Eco57MI     FalI    Hin4I
   HincII     HpaI     MmeI   MspA1I    PflMI     PstI    PvuII     SfcI
     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI