Restriction map for
pFN21AE3405
(Linear) (Six-base) MAPSORT of: KIEE3405.seq Check: 1276 from: 1 to: 636
KIEE3405.orf
With 182 enzymes: SgfI *
March 22, 2012 16:31 ..
AatII G_ACGT'C
Cuts at: 0 484 636
Size: 484 152
AccI GT'mk_AC
Cuts at: 0 158 636
Size: 158 478
AfeI AGC'GCT
Cuts at: 0 109 636
Size: 109 527
AlwNI CAG_nnn'CTG
Cuts at: 0 381 444 636
Size: 381 63 192
Fragments arranged by size:
381 192 63
ApaI G_GGCC'C
Cuts at: 0 16 559 636
Size: 16 543 77
Fragments arranged by size:
543 77 16
AvaI C'yCGr_G
Cuts at: 0 21 495 636
Size: 21 474 141
Fragments arranged by size:
474 141 21
BanI G'GyrC_C
Cuts at: 0 121 324 636
Size: 121 203 312
Fragments arranged by size:
312 203 121
BanII G_rGCy'C
Cuts at: 0 16 313 364 539 559 580 636
Size: 16 297 51 175 20 21 56
Fragments arranged by size:
297 175 56 51 21 20 16
BbeI G_GCGC'C
Cuts at: 0 125 636
Size: 125 511
BbsI GAAGACnn'nnnn_
Cuts at: 0 178 636
Size: 178 458
BbvCI CC'TCA_GC
Cuts at: 0 403 431 636
Size: 403 28 205
Fragments arranged by size:
403 205 28
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 591 625 636
Size: 591 34 11
Fragments arranged by size:
591 34 11
BglI GCCn_nnn'nGGC
Cuts at: 0 277 636
Size: 277 359
BglII A'GATC_T
Cuts at: 0 209 636
Size: 209 427
BlpI GC'TnA_GC
Cuts at: 0 449 636
Size: 449 187
Bme1580I G_kGCm'C
Cuts at: 0 16 559 636
Size: 16 543 77
Fragments arranged by size:
543 77 16
BmgBI CAC'GTC
Cuts at: 0 95 636
Size: 95 541
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 636
Size: 199 437
Bpu10I CC'TnA_GC
Cuts at: 0 403 431 636
Size: 403 28 205
Fragments arranged by size:
403 205 28
BsaI GGTCTCn'nnnn_
Cuts at: 0 426 636
Size: 426 210
BsaHI Gr'CG_yC
Cuts at: 0 122 155 481 636
Size: 122 33 326 155
Fragments arranged by size:
326 155 122 33
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 321 523 636
Size: 321 202 113
Fragments arranged by size:
321 202 113
BseYI C'CCAG_C
Cuts at: 0 620 636
Size: 620 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 394 636
Size: 124 270 242
Fragments arranged by size:
270 242 124
BsiEI CG_ry'CG
Cuts at: 0 224 636
Size: 224 412
BsiHKAI G_wGCw'C
Cuts at: 0 313 455 539 636
Size: 313 142 84 97
Fragments arranged by size:
313 142 97 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 472 636
Size: 472 164
Bsp1286I G_dGCh'C
Cuts at: 0 16 313 364 455 539 559 580 636
Size: 16 297 51 91 84 20 21 56
Fragments arranged by size:
297 91 84 56 51 21 20 16
BsrBI CCG'CTC
Cuts at: 0 234 636
Size: 234 402
BsrFI r'CCGG_y
Cuts at: 0 118 223 636
Size: 118 105 413
Fragments arranged by size:
413 118 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 95 636
Size: 95 541
BstYI r'GATC_y
Cuts at: 0 182 209 636
Size: 182 27 427
Fragments arranged by size:
427 182 27
Bsu36I CC'TnA_GG
Cuts at: 0 520 636
Size: 520 116
BtgI C'CryG_G
Cuts at: 0 530 636
Size: 530 106
BtsI GCAGTG_nn'
Cuts at: 0 273 636
Size: 273 363
EaeI y'GGCC_r
Cuts at: 0 221 225 527 636
Size: 221 4 302 109
Fragments arranged by size:
302 221 109 4
EagI C'GGCC_G
Cuts at: 0 221 636
Size: 221 415
EarI CTCTTCn'nnn_
Cuts at: 0 568 636
Size: 568 68
EcoICRI GAG'CTC
Cuts at: 0 311 537 636
Size: 311 226 99
Fragments arranged by size:
311 226 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 636
Size: 199 437
EcoNI CCTnn'n_nnAGG
Cuts at: 0 519 636
Size: 519 117
EcoO109I rG'GnC_Cy
Cuts at: 0 27 555 556 636
Size: 27 528 1 80
Fragments arranged by size:
528 80 27 1
FseI GG_CCGG'CC
Cuts at: 0 227 636
Size: 227 409
HaeII r_GCGC'y
Cuts at: 0 111 125 138 636
Size: 111 14 13 498
Fragments arranged by size:
498 111 14 13
HincII GTy'rAC
Cuts at: 0 159 636
Size: 159 477
KasI G'GCGC_C
Cuts at: 0 121 636
Size: 121 515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 636
Size: 389 247
MscI TGG'CCA
Cuts at: 0 529 636
Size: 529 107
MspA1I CmG'CkG
Cuts at: 0 378 594 636
Size: 378 216 42
Fragments arranged by size:
378 216 42
NaeI GCC'GGC
Cuts at: 0 225 636
Size: 225 411
NarI GG'CG_CC
Cuts at: 0 122 636
Size: 122 514
NgoMIV G'CCGG_C
Cuts at: 0 223 636
Size: 223 413
PpuMI rG'GwC_Cy
Cuts at: 0 27 636
Size: 27 609
PspOMI G'GGCC_C
Cuts at: 0 12 555 636
Size: 12 543 81
Fragments arranged by size:
543 81 12
PspXI vC'TCGA_Gb
Cuts at: 0 495 636
Size: 495 141
PstI C_TGCA'G
Cuts at: 0 209 636
Size: 209 427
PvuII CAG'CTG
Cuts at: 0 378 594 636
Size: 378 216 42
Fragments arranged by size:
378 216 42
SacI G_AGCT'C
Cuts at: 0 313 539 636
Size: 313 226 97
Fragments arranged by size:
313 226 97
SalI G'TCGA_C
Cuts at: 0 157 636
Size: 157 479
SanDI GG'GwC_CC
Cuts at: 0 27 636
Size: 27 609
SapI GCTCTTCn'nnn_
Cuts at: 0 568 636
Size: 568 68
SfcI C'TryA_G
Cuts at: 0 205 636
Size: 205 431
SfoI GGC'GCC
Cuts at: 0 123 636
Size: 123 513
SmaI CCC'GGG
Cuts at: 0 23 636
Size: 23 613
SmlI C'TyrA_G
Cuts at: 0 495 636
Size: 495 141
StuI AGG'CCT
Cuts at: 0 514 636
Size: 514 122
StyI C'CwwG_G
Cuts at: 0 425 488 636
Size: 425 63 148
Fragments arranged by size:
425 148 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 265 636
Size: 265 371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 174 636
Size: 174 462
XhoI C'TCGA_G
Cuts at: 0 495 636
Size: 495 141
XmaI C'CCGG_G
Cuts at: 0 21 636
Size: 21 615
ZraI GAC'GTC
Cuts at: 0 482 636
Size: 482 154
Enzymes that do cut:
AatII AccI AfeI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I
BmgBI BpmI Bpu10I BsaI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI BstAPI BstYI
Bsu36I BtgI BtsI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FseI HaeII HincII KasI MmeI MscI
MspA1I NaeI NarI NgoMIV PpuMI PspOMI PspXI PstI
PvuII SacI SalI SanDI SapI SfcI SfoI SmaI
SmlI StuI StyI TaqII XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BmrI BmtI
BplI BpuEI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EciI EcoRI EcoRV FalI FspI FspAI Hin4I
HindIII HpaI KpnI MfeI MluI MslI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SacII SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XmnI