Restriction map for
pFN21AE3396
(Linear) (Six-base) MAPSORT of: KIEE3396.seq Check: 8717 from: 1 to: 3825
KIEE3396.orf
With 182 enzymes: SgfI *
January 29, 2014 11:45 ..
AccI GT'mk_AC
Cuts at: 0 960 2651 3825
Size: 960 1691 1174
Fragments arranged by size:
1691 1174 960
Acc65I G'GTAC_C
Cuts at: 0 1958 3825
Size: 1958 1867
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 532 734 1677 2545 3825
Size: 424 108 202 943 868 1280
Fragments arranged by size:
1280 943 868 424 202 108
AflIII A'CryG_T
Cuts at: 0 2126 3825
Size: 2126 1699
AhdI GACnn_n'nnGTC
Cuts at: 0 606 3825
Size: 606 3219
AleI CACnn'nnGTG
Cuts at: 0 980 1114 3825
Size: 980 134 2711
Fragments arranged by size:
2711 980 134
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3745 3825
Size: 3745 80
AlwNI CAG_nnn'CTG
Cuts at: 0 1212 2439 3825
Size: 1212 1227 1386
Fragments arranged by size:
1386 1227 1212
ApoI r'AATT_y
Cuts at: 0 298 444 1309 2704 3602 3825
Size: 298 146 865 1395 898 223
Fragments arranged by size:
1395 898 865 298 223 146
AseI AT'TA_AT
Cuts at: 0 761 1678 3825
Size: 761 917 2147
Fragments arranged by size:
2147 917 761
AvaI C'yCGr_G
Cuts at: 0 1445 3825
Size: 1445 2380
AvrII C'CTAG_G
Cuts at: 0 370 3825
Size: 370 3455
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1988 2021 3825
Size: 1988 33 1804
Fragments arranged by size:
1988 1804 33
BanI G'GyrC_C
Cuts at: 0 1832 1958 3825
Size: 1832 126 1867
Fragments arranged by size:
1867 1832 126
BanII G_rGCy'C
Cuts at: 0 1631 3738 3825
Size: 1631 2107 87
Fragments arranged by size:
2107 1631 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 1168 3440 3825
Size: 1168 2272 385
Fragments arranged by size:
2272 1168 385
BciVI GTATCCnnnnn_n'
Cuts at: 0 2759 3825
Size: 2759 1066
BclI T'GATC_A
Cuts at: 0 3677 3825
Size: 3677 148
BfrBI ATG'CAT
Cuts at: 0 2240 3825
Size: 2240 1585
BglII A'GATC_T
Cuts at: 0 689 882 1251 3825
Size: 689 193 369 2574
Fragments arranged by size:
2574 689 369 193
BlpI GC'TnA_GC
Cuts at: 0 1625 3825
Size: 1625 2200
Bme1580I G_kGCm'C
Cuts at: 0 977 3824 3825
Size: 977 2847 1
Fragments arranged by size:
2847 977 1
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 1578 2970 3001 3825
Size: 144 1434 1392 31 824
Fragments arranged by size:
1434 1392 824 144 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 875 1615 2345 3760 3825
Size: 579 296 740 730 1415 65
Fragments arranged by size:
1415 740 730 579 296 65
Bpu10I CC'TnA_GC
Cuts at: 0 1913 3408 3825
Size: 1913 1495 417
Fragments arranged by size:
1913 1495 417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1069 2573 3694 3825
Size: 248 821 1504 1121 131
Fragments arranged by size:
1504 1121 821 248 131
BsaAI yAC'GTr
Cuts at: 0 1596 3825
Size: 1596 2229
BsaBI GATnn'nnATC
Cuts at: 0 2332 3825
Size: 2332 1493
BsaWI w'CCGG_w
Cuts at: 0 3367 3825
Size: 3367 458
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2758 2788 3825
Size: 2758 30 1037
Fragments arranged by size:
2758 1037 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 37 500 1160 1504 3825
Size: 37 463 660 344 2321
Fragments arranged by size:
2321 660 463 344 37
BseYI C'CCAG_C
Cuts at: 0 1793 3825
Size: 1793 2032
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 2452 3825
Size: 216 2236 1373
Fragments arranged by size:
2236 1373 216
BsiHKAI G_wGCw'C
Cuts at: 0 1408 1631 2987 3825
Size: 1408 223 1356 838
Fragments arranged by size:
1408 1356 838 223
BsmI GAATG_Cn'
Cuts at: 0 347 828 3134 3720 3825
Size: 347 481 2306 586 105
Fragments arranged by size:
2306 586 481 347 105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 148 3825
Size: 148 3677
Bsp1286I G_dGCh'C
Cuts at: 0 977 1408 1631 2987 3738 3824 3825
Size: 977 431 223 1356 751 86 1
Fragments arranged by size:
1356 977 751 431 223 86 1
BspHI T'CATG_A
Cuts at: 0 183 3825
Size: 183 3642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3257 3446 3825
Size: 3257 189 379
Fragments arranged by size:
3257 379 189
BsrBI CCG'CTC
Cuts at: 0 3744 3825
Size: 3744 81
BsrDI GCAATG_nn'
Cuts at: 0 2391 3825
Size: 2391 1434
BsrGI T'GTAC_A
Cuts at: 0 2330 3825
Size: 2330 1495
BssSI C'ACGA_G
Cuts at: 0 2884 3825
Size: 2884 941
BstAPI GCAn_nnn'nTGC
Cuts at: 0 419 2439 3351 3825
Size: 419 2020 912 474
Fragments arranged by size:
2020 912 474 419
BstBI TT'CG_AA
Cuts at: 0 3199 3825
Size: 3199 626
BstEII G'GTnAC_C
Cuts at: 0 1489 3825
Size: 1489 2336
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2406 2899 3403 3817 3825
Size: 2406 493 504 414 8
Fragments arranged by size:
2406 504 493 414 8
BstYI r'GATC_y
Cuts at: 0 541 689 702 789 882 1251 2409 3504
Size: 541 148 13 87 93 369 1158 1095
Cuts at: 3504 3825
Size: 321
Fragments arranged by size:
1158 1095 541 369 321 148 93 87
13
Bsu36I CC'TnA_GG
Cuts at: 0 1037 3422 3825
Size: 1037 2385 403
Fragments arranged by size:
2385 1037 403
BtgI C'CryG_G
Cuts at: 0 2777 3825
Size: 2777 1048
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3055 3825
Size: 3055 770
BtsI GCAGTG_nn'
Cuts at: 0 1109 1121 1976 2141 3316 3487 3825
Size: 1109 12 855 165 1175 171 338
Fragments arranged by size:
1175 1109 855 338 171 165 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2766 2801 3056 3091 3825
Size: 2766 35 255 35 734
Fragments arranged by size:
2766 734 255 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2430 3272 3825
Size: 2430 842 553
Fragments arranged by size:
2430 842 553
DrdI GACnn_nn'nnGTC
Cuts at: 0 1199 3233 3825
Size: 1199 2034 592
Fragments arranged by size:
2034 1199 592
EaeI y'GGCC_r
Cuts at: 0 1502 3825
Size: 1502 2323
EarI CTCTTCn'nnn_
Cuts at: 0 27 446 1423 1844 2597 3825
Size: 27 419 977 421 753 1228
Fragments arranged by size:
1228 977 753 421 419 27
EcoICRI GAG'CTC
Cuts at: 0 1629 3825
Size: 1629 2196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 532 579 734 875 1615 1677 2345
Size: 424 108 47 155 141 740 62 668
Cuts at: 2345 2545 3760 3825
Size: 200 1215 65
Fragments arranged by size:
1215 740 668 424 200 155 141 108
65 62 47
EcoO109I rG'GnC_Cy
Cuts at: 0 391 1209 1439 1945 3417 3633 3825
Size: 391 818 230 506 1472 216 192
Fragments arranged by size:
1472 818 506 391 230 216 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 399 824 2626 3825
Size: 399 425 1802 1199
Fragments arranged by size:
1802 1199 425 399
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 592 624 853 885 1155 1187 1201 1220
Size: 592 32 229 32 270 32 14 19
Cuts at: 1220 1233 1252 2215 2247 2758 2790 3297 3329
Size: 13 19 963 32 511 32 507 32
Cuts at: 3329 3511 3543 3568 3600 3825
Size: 182 32 25 32 225
Fragments arranged by size:
963 592 511 507 270 229 225 182
32 32 32 32 32 32 32 32
25 19 19 14 13
HincII GTy'rAC
Cuts at: 0 988 2132 2787 3825
Size: 988 1144 655 1038
Fragments arranged by size:
1144 1038 988 655
HindIII A'AGCT_T
Cuts at: 0 743 845 3649 3825
Size: 743 102 2804 176
Fragments arranged by size:
2804 743 176 102
KpnI G_GTAC'C
Cuts at: 0 1962 3825
Size: 1962 1863
MfeI C'AATT_G
Cuts at: 0 1057 1534 1658 3825
Size: 1057 477 124 2167
Fragments arranged by size:
2167 1057 477 124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 3825
Size: 1045 2780
MscI TGG'CCA
Cuts at: 0 1504 3825
Size: 1504 2321
MslI CAynn'nnrTG
Cuts at: 0 980 1114 2146 3531 3825
Size: 980 134 1032 1385 294
Fragments arranged by size:
1385 1032 980 294 134
MspA1I CmG'CkG
Cuts at: 0 1624 2523 3555 3825
Size: 1624 899 1032 270
Fragments arranged by size:
1624 1032 899 270
NdeI CA'TA_TG
Cuts at: 0 3018 3825
Size: 3018 807
NruI TCG'CGA
Cuts at: 0 3040 3825
Size: 3040 785
NsiI A_TGCA'T
Cuts at: 0 2242 3825
Size: 2242 1583
NspI r_CATG'y
Cuts at: 0 2130 2240 3354 3825
Size: 2130 110 1114 471
Fragments arranged by size:
2130 1114 471 110
PasI CC'CwG_GG
Cuts at: 0 1920 3754 3825
Size: 1920 1834 71
Fragments arranged by size:
1920 1834 71
PciI A'CATG_T
Cuts at: 0 2126 3825
Size: 2126 1699
PflMI CCAn_nnn'nTGG
Cuts at: 0 1500 1732 1955 2832 3825
Size: 1500 232 223 877 993
Fragments arranged by size:
1500 993 877 232 223
PfoI T'CCnGG_A
Cuts at: 0 1631 3825
Size: 1631 2194
PmlI CAC'GTG
Cuts at: 0 1596 3825
Size: 1596 2229
PpuMI rG'GwC_Cy
Cuts at: 0 1209 1945 3417 3633 3825
Size: 1209 736 1472 216 192
Fragments arranged by size:
1472 1209 736 216 192
PshAI GACnn'nnGTC
Cuts at: 0 49 3825
Size: 49 3776
PsiI TTA'TAA
Cuts at: 0 2285 3825
Size: 2285 1540
PstI C_TGCA'G
Cuts at: 0 827 1116 3432 3825
Size: 827 289 2316 393
Fragments arranged by size:
2316 827 393 289
PvuII CAG'CTG
Cuts at: 0 1624 2523 3555 3825
Size: 1624 899 1032 270
Fragments arranged by size:
1624 1032 899 270
SacI G_AGCT'C
Cuts at: 0 1631 3825
Size: 1631 2194
SapI GCTCTTCn'nnn_
Cuts at: 0 1423 1844 3825
Size: 1423 421 1981
Fragments arranged by size:
1981 1423 421
SfcI C'TryA_G
Cuts at: 0 823 1112 2549 2648 3428 3825
Size: 823 289 1437 99 780 397
Fragments arranged by size:
1437 823 780 397 289 99
SmlI C'TyrA_G
Cuts at: 0 263 1048 2588 3709 3825
Size: 263 785 1540 1121 116
Fragments arranged by size:
1540 1121 785 263 116
SphI G_CATG'C
Cuts at: 0 3354 3825
Size: 3354 471
SspI AAT'ATT
Cuts at: 0 171 1325 3825
Size: 171 1154 2500
Fragments arranged by size:
2500 1154 171
StuI AGG'CCT
Cuts at: 0 120 3825
Size: 120 3705
StyI C'CwwG_G
Cuts at: 0 370 932 1583 1731 1805 2471 2804 2813
Size: 370 562 651 148 74 666 333 9
Cuts at: 2813 3626 3791 3825
Size: 813 165 34
Fragments arranged by size:
813 666 651 562 370 333 165 148
74 34 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1774 2443 2513 3825
Size: 1774 669 70 1312
Fragments arranged by size:
1774 1312 669 70
TatI w'GTAC_w
Cuts at: 0 100 2123 2330 3825
Size: 100 2023 207 1495
Fragments arranged by size:
2023 1495 207 100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3273 3825
Size: 3273 552
XmnI GAAnn'nnTTC
Cuts at: 0 2606 3825
Size: 2606 1219
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AleI AlfI AlwNI
ApoI AseI AvaI AvrII BaeI BanI BanII BbsI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EarI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII HindIII
KpnI MfeI MmeI MscI MslI MspA1I NdeI NruI
NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI
PshAI PsiI PstI PvuII SacI SapI SfcI SmlI
SphI SspI StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AloI ApaI
ApaLI AscI AsiSI BamHI BbeI BbvCI BcgI BglI
BmgBI BmtI BplI BsaI BsaHI BsiEI BsiWI BspEI
BsrFI BssHII BstZ17I ClaI DraI EagI EciI EcoNI
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
MluI NaeI NarI NcoI NgoMIV NheI NotI PacI
PmeI PpiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TstI Tth111I
XbaI XhoI XmaI ZraI