Restriction map for
pFN21AE3390
(Linear) (Six-base) MAPSORT of: KIEE3390.seq Check: 7446 from: 1 to: 3612
KIEE3390.orf
With 182 enzymes: SgfI *
January 18, 2013 16:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 325 3612
Size: 325 3287
AatII G_ACGT'C
Cuts at: 0 2484 3612
Size: 2484 1128
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 487 617 947 1064 1136 1484 1931
Size: 227 260 130 330 117 72 348 447
Cuts at: 1931 1964 2102 2480 2494 2785 3292 3418 3612
Size: 33 138 378 14 291 507 126 194
Fragments arranged by size:
507 447 378 348 330 291 260 227
194 138 130 126 117 72 33 14
AflII C'TTAA_G
Cuts at: 0 3022 3612
Size: 3022 590
AflIII A'CryG_T
Cuts at: 0 66 1651 3612
Size: 66 1585 1961
Fragments arranged by size:
1961 1585 66
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1988 2020 2561 2593 3612
Size: 1988 32 541 32 1019
Fragments arranged by size:
1988 1019 541 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 858 2218 2254 3396 3612
Size: 858 1360 36 1142 216
Fragments arranged by size:
1360 1142 858 216 36
ApoI r'AATT_y
Cuts at: 0 325 789 984 1136 2054 2289 2389 2573
Size: 325 464 195 152 918 235 100 184
Cuts at: 2573 2749 3574 3612
Size: 176 825 38
Fragments arranged by size:
918 825 464 325 235 195 184 176
152 100 38
AseI AT'TA_AT
Cuts at: 0 1190 1331 1750 1754 2138 3612
Size: 1190 141 419 4 384 1474
Fragments arranged by size:
1474 1190 419 384 141 4
AvaI C'yCGr_G
Cuts at: 0 2915 3612
Size: 2915 697
AvrII C'CTAG_G
Cuts at: 0 3457 3612
Size: 3457 155
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1014 1047 3612
Size: 1014 33 2565
Fragments arranged by size:
2565 1014 33
BamHI G'GATC_C
Cuts at: 0 314 3612
Size: 314 3298
BanII G_rGCy'C
Cuts at: 0 2532 2916 2939 3612
Size: 2532 384 23 673
Fragments arranged by size:
2532 673 384 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 98 776 1316 1580 1604 1694 2364 3612
Size: 98 678 540 264 24 90 670 1248
Fragments arranged by size:
1248 678 670 540 264 98 90 24
BbvCI CC'TCA_GC
Cuts at: 0 1697 2114 3612
Size: 1697 417 1498
Fragments arranged by size:
1697 1498 417
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2674 2708 3612
Size: 2674 34 904
Fragments arranged by size:
2674 904 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1695 3612
Size: 1695 1917
BclI T'GATC_A
Cuts at: 0 258 1622 3612
Size: 258 1364 1990
Fragments arranged by size:
1990 1364 258
BfrBI ATG'CAT
Cuts at: 0 424 3612
Size: 424 3188
BglII A'GATC_T
Cuts at: 0 23 462 3303 3612
Size: 23 439 2841 309
Fragments arranged by size:
2841 439 309 23
BlpI GC'TnA_GC
Cuts at: 0 3272 3612
Size: 3272 340
BmrI ACTGGGnnnn_n'
Cuts at: 0 127 2126 3536 3612
Size: 127 1999 1410 76
Fragments arranged by size:
1999 1410 127 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1104 3287 3612
Size: 1104 2183 325
Fragments arranged by size:
2183 1104 325
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1538 3612
Size: 1538 2074
Bpu10I CC'TnA_GC
Cuts at: 0 1697 2114 3612
Size: 1697 417 1498
Fragments arranged by size:
1697 1498 417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 1493 3086 3612
Size: 388 1105 1593 526
Fragments arranged by size:
1593 1105 526 388
BsaI GGTCTCn'nnnn_
Cuts at: 0 157 366 3447 3612
Size: 157 209 3081 165
Fragments arranged by size:
3081 209 165 157
BsaAI yAC'GTr
Cuts at: 0 69 3612
Size: 69 3543
BsaBI GATnn'nnATC
Cuts at: 0 2337 3612
Size: 2337 1275
BsaHI Gr'CG_yC
Cuts at: 0 2481 3612
Size: 2481 1131
BsaWI w'CCGG_w
Cuts at: 0 3604 3612
Size: 3604 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 985 3612
Size: 985 2627
BseYI C'CCAG_C
Cuts at: 0 3589 3612
Size: 3589 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 3218 3612
Size: 353 2865 394
Fragments arranged by size:
2865 394 353
BsiHKAI G_wGCw'C
Cuts at: 0 2687 2916 2939 3612
Size: 2687 229 23 673
Fragments arranged by size:
2687 673 229 23
BsmI GAATG_Cn'
Cuts at: 0 3544 3597 3612
Size: 3544 53 15
Fragments arranged by size:
3544 53 15
Bsp1286I G_dGCh'C
Cuts at: 0 2532 2687 2916 2939 3612
Size: 2532 155 229 23 673
Fragments arranged by size:
2532 673 229 155 23
BspHI T'CATG_A
Cuts at: 0 705 2906 3612
Size: 705 2201 706
Fragments arranged by size:
2201 706 705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 325 1008 3612
Size: 325 683 2604
Fragments arranged by size:
2604 683 325
BsrDI GCAATG_nn'
Cuts at: 0 1229 3612
Size: 1229 2383
BsrGI T'GTAC_A
Cuts at: 0 143 3612
Size: 143 3469
BssSI C'ACGA_G
Cuts at: 0 540 3031 3612
Size: 540 2491 581
Fragments arranged by size:
2491 581 540
BstAPI GCAn_nnn'nTGC
Cuts at: 0 349 3348 3612
Size: 349 2999 264
Fragments arranged by size:
2999 349 264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 779 3612
Size: 779 2833
BstYI r'GATC_y
Cuts at: 0 23 314 462 3303 3612
Size: 23 291 148 2841 309
Fragments arranged by size:
2841 309 291 148 23
Bsu36I CC'TnA_GG
Cuts at: 0 1775 3612
Size: 1775 1837
BtgI C'CryG_G
Cuts at: 0 1763 3612
Size: 1763 1849
BtsI GCAGTG_nn'
Cuts at: 0 2261 3141 3322 3612
Size: 2261 880 181 290
Fragments arranged by size:
2261 880 290 181
ClaI AT'CG_AT
Cuts at: 0 2335 3612
Size: 2335 1277
DraI TTT'AAA
Cuts at: 0 3519 3612
Size: 3519 93
DrdI GACnn_nn'nnGTC
Cuts at: 0 3531 3612
Size: 3531 81
EaeI y'GGCC_r
Cuts at: 0 3155 3612
Size: 3155 457
EarI CTCTTCn'nnn_
Cuts at: 0 67 552 962 1270 2513 3163 3473 3612
Size: 67 485 410 308 1243 650 310 139
Fragments arranged by size:
1243 650 485 410 310 308 139 67
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 529 3612
Size: 529 3083
EcoICRI GAG'CTC
Cuts at: 0 2914 2937 3612
Size: 2914 23 675
Fragments arranged by size:
2914 675 23
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 487 617 947 1064 1136 1484 1538
Size: 227 260 130 330 117 72 348 54
Cuts at: 1538 1931 1964 2102 2480 2494 2785 3292 3418
Size: 393 33 138 378 14 291 507 126
Cuts at: 3418 3612
Size: 194
Fragments arranged by size:
507 393 378 348 330 291 260 227
194 138 130 126 117 72 54 33
14
EcoO109I rG'GnC_Cy
Cuts at: 0 78 1771 3612
Size: 78 1693 1841
Fragments arranged by size:
1841 1693 78
EcoRV GAT'ATC
Cuts at: 0 504 2339 3612
Size: 504 1835 1273
Fragments arranged by size:
1835 1273 504
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 232 831 1072 1579 3612
Size: 232 599 241 507 2033
Fragments arranged by size:
2033 599 507 241 232
HaeII r_GCGC'y
Cuts at: 0 2586 3030 3612
Size: 2586 444 582
Fragments arranged by size:
2586 582 444
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 640 672 834 866 2317 2349
Size: 187 32 421 32 162 32 1451 32
Cuts at: 2349 3612
Size: 1263
Fragments arranged by size:
1451 1263 421 187 162 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 277 1285 1450 3612
Size: 277 1008 165 2162
Fragments arranged by size:
2162 1008 277 165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 3612
Size: 911 2701
MscI TGG'CCA
Cuts at: 0 3157 3612
Size: 3157 455
MslI CAynn'nnrTG
Cuts at: 0 777 3612
Size: 777 2835
MspA1I CmG'CkG
Cuts at: 0 855 2970 3348 3612
Size: 855 2115 378 264
Fragments arranged by size:
2115 855 378 264
NcoI C'CATG_G
Cuts at: 0 1763 3612
Size: 1763 1849
NsiI A_TGCA'T
Cuts at: 0 426 3612
Size: 426 3186
NspI r_CATG'y
Cuts at: 0 1655 3612
Size: 1655 1957
PacI TTA_AT'TAA
Cuts at: 0 1754 3612
Size: 1754 1858
PasI CC'CwG_GG
Cuts at: 0 660 3612
Size: 660 2952
PciI A'CATG_T
Cuts at: 0 1651 3612
Size: 1651 1961
PflMI CCAn_nnn'nTGG
Cuts at: 0 3372 3612
Size: 3372 240
PmlI CAC'GTG
Cuts at: 0 69 3612
Size: 69 3543
PpuMI rG'GwC_Cy
Cuts at: 0 78 1771 3612
Size: 78 1693 1841
Fragments arranged by size:
1841 1693 78
PsiI TTA'TAA
Cuts at: 0 1456 3612
Size: 1456 2156
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2936 2968 3612
Size: 2936 32 644
Fragments arranged by size:
2936 644 32
PstI C_TGCA'G
Cuts at: 0 863 3353 3612
Size: 863 2490 259
Fragments arranged by size:
2490 863 259
PvuII CAG'CTG
Cuts at: 0 855 2970 3348 3612
Size: 855 2115 378 264
Fragments arranged by size:
2115 855 378 264
SacI G_AGCT'C
Cuts at: 0 2916 2939 3612
Size: 2916 23 673
Fragments arranged by size:
2916 673 23
SapI GCTCTTCn'nnn_
Cuts at: 0 2513 3612
Size: 2513 1099
SfcI C'TryA_G
Cuts at: 0 859 2090 2554 3349 3496 3612
Size: 859 1231 464 795 147 116
Fragments arranged by size:
1231 859 795 464 147 116
SmlI C'TyrA_G
Cuts at: 0 367 1508 2915 3022 3101 3612
Size: 367 1141 1407 107 79 511
Fragments arranged by size:
1407 1141 511 367 107 79
SspI AAT'ATT
Cuts at: 0 1386 1536 2871 3612
Size: 1386 150 1335 741
Fragments arranged by size:
1386 1335 741 150
StyI C'CwwG_G
Cuts at: 0 1763 3457 3612
Size: 1763 1694 155
Fragments arranged by size:
1763 1694 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 1594 3612
Size: 278 1316 2018
Fragments arranged by size:
2018 1316 278
TatI w'GTAC_w
Cuts at: 0 143 3612
Size: 143 3469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 823 855 3612
Size: 823 32 2757
Fragments arranged by size:
2757 823 32
XbaI T'CTAG_A
Cuts at: 0 282 2640 3612
Size: 282 2358 972
Fragments arranged by size:
2358 972 282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3150 3612
Size: 3150 462
XhoI C'TCGA_G
Cuts at: 0 2915 3612
Size: 2915 697
XmnI GAAnn'nnTTC
Cuts at: 0 561 2201 3612
Size: 561 1640 1411
Fragments arranged by size:
1640 1411 561
ZraI GAC'GTC
Cuts at: 0 2482 3612
Size: 2482 1130
Enzymes that do cut:
AarI AatII AcuI AflII AflIII AloI AlwNI ApoI
AseI AvaI AvrII BaeI BamHI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI ClaI DraI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NcoI NsiI NspI PacI PasI
PciI PflMI PmlI PpuMI PsiI PsrI PstI PvuII
SacI SapI SfcI SmlI SspI StyI TaqII TatI
TstI XbaI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AgeI AhdI AleI AlfI
ApaI ApaLI AscI AsiSI BanI BbeI BglI Bme1580I
BmgBI BmtI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BstBI BstEII BstZ17I BtgZI CspCI DraIII
EagI EcoNI EcoRI FseI FspI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PfoI PmeI PpiI PshAI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XmaI