(Linear) (Six-base) MAPSORT of: KIEE3363.seq Check: 6585 from: 1 to: 3210 KIEE3363.orf With 182 enzymes: SgfI * November 16, 2016 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1177 1996 3210 Size: 1177 819 1214 Fragments arranged by size: 1214 1177 819 AccI GT'mk_AC Cuts at: 0 1106 1163 3210 Size: 1106 57 2047 Fragments arranged by size: 2047 1106 57 Acc65I G'GTAC_C Cuts at: 0 79 1433 3210 Size: 79 1354 1777 Fragments arranged by size: 1777 1354 79 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 1264 3144 3210 Size: 412 852 1880 66 Fragments arranged by size: 1880 852 412 66 AfeI AGC'GCT Cuts at: 0 2185 3124 3210 Size: 2185 939 86 Fragments arranged by size: 2185 939 86 AflIII A'CryG_T Cuts at: 0 183 3210 Size: 183 3027 AhdI GACnn_n'nnGTC Cuts at: 0 1207 3210 Size: 1207 2003 AleI CACnn'nnGTG Cuts at: 0 1533 3210 Size: 1533 1677 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 712 744 1354 1386 3210 Size: 712 32 610 32 1824 Fragments arranged by size: 1824 712 610 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 382 2520 2658 3210 Size: 382 2138 138 552 Fragments arranged by size: 2138 552 382 138 ApaI G_GGCC'C Cuts at: 0 178 210 925 2077 3135 3210 Size: 178 32 715 1152 1058 75 Fragments arranged by size: 1152 1058 715 178 75 32 AvaI C'yCGr_G Cuts at: 0 308 759 1673 1682 2215 2923 3085 3210 Size: 308 451 914 9 533 708 162 125 Fragments arranged by size: 914 708 533 451 308 162 125 9 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 172 205 1425 1458 3210 Size: 172 33 1220 33 1752 Fragments arranged by size: 1752 1220 172 33 33 BamHI G'GATC_C Cuts at: 0 16 3210 Size: 16 3194 BanI G'GyrC_C Cuts at: 0 79 137 289 461 687 1433 1563 2128 Size: 79 58 152 172 226 746 130 565 Cuts at: 2128 2901 3210 Size: 773 309 Fragments arranged by size: 773 746 565 309 226 172 152 130 79 58 BanII G_rGCy'C Cuts at: 0 178 210 925 1055 2077 2320 2609 3135 Size: 178 32 715 130 1022 243 289 526 Cuts at: 3135 3210 Size: 75 Fragments arranged by size: 1022 715 526 289 243 178 130 75 32 BbeI G_GCGC'C Cuts at: 0 141 3210 Size: 141 3069 BbsI GAAGACnn'nnnn_ Cuts at: 0 90 974 1371 3210 Size: 90 884 397 1839 Fragments arranged by size: 1839 884 397 90 BbvCI CC'TCA_GC Cuts at: 0 2277 2704 2851 3080 3136 3210 Size: 2277 427 147 229 56 74 Fragments arranged by size: 2277 427 229 147 74 56 BglI GCCn_nnn'nGGC Cuts at: 0 1186 1239 2137 3210 Size: 1186 53 898 1073 Fragments arranged by size: 1186 1073 898 53 BlpI GC'TnA_GC Cuts at: 0 49 2603 3210 Size: 49 2554 607 Fragments arranged by size: 2554 607 49 Bme1580I G_kGCm'C Cuts at: 0 178 210 464 925 1235 1568 1746 2077 Size: 178 32 254 461 310 333 178 331 Cuts at: 2077 2820 3135 3210 Size: 743 315 75 Fragments arranged by size: 743 461 333 331 315 310 254 178 178 75 32 BmgBI CAC'GTC Cuts at: 0 699 1959 3210 Size: 699 1260 1251 Fragments arranged by size: 1260 1251 699 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 1288 2956 3210 Size: 344 944 1668 254 Fragments arranged by size: 1668 944 344 254 Bpu10I CC'TnA_GC Cuts at: 0 253 575 2277 2444 2704 2851 3080 3136 Size: 253 322 1702 167 260 147 229 56 Cuts at: 3136 3210 Size: 74 Fragments arranged by size: 1702 322 260 253 229 167 147 74 56 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 669 1489 2632 3210 Size: 669 820 1143 578 Fragments arranged by size: 1143 820 669 578 BsaI GGTCTCn'nnnn_ Cuts at: 0 857 1844 2464 2636 3210 Size: 857 987 620 172 574 Fragments arranged by size: 987 857 620 574 172 BsaBI GATnn'nnATC Cuts at: 0 1351 1924 3210 Size: 1351 573 1286 Fragments arranged by size: 1351 1286 573 BsaHI Gr'CG_yC Cuts at: 0 138 3210 Size: 138 3072 BsaWI w'CCGG_w Cuts at: 0 2528 3210 Size: 2528 682 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 712 742 2746 2776 3139 3169 3210 Size: 712 30 2004 30 363 30 41 Fragments arranged by size: 2004 712 363 41 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 733 1027 1285 2077 2239 2470 3210 Size: 733 294 258 792 162 231 740 Fragments arranged by size: 792 740 733 294 258 231 162 BseYI C'CCAG_C Cuts at: 0 72 749 1054 1455 1523 1654 1908 2157 Size: 72 677 305 401 68 131 254 249 Cuts at: 2157 2403 3061 3128 3210 Size: 246 658 67 82 Fragments arranged by size: 677 658 401 305 254 249 246 131 82 72 68 67 BsiHKAI G_wGCw'C Cuts at: 0 1073 2759 3210 Size: 1073 1686 451 Fragments arranged by size: 1686 1073 451 BsiWI C'GTAC_G Cuts at: 0 1798 3210 Size: 1798 1412 BsmI GAATG_Cn' Cuts at: 0 2245 3210 Size: 2245 965 Bsp1286I G_dGCh'C Cuts at: 0 178 210 464 925 1055 1073 1235 1568 Size: 178 32 254 461 130 18 162 333 Cuts at: 1568 1746 2077 2320 2609 2759 2820 3135 3210 Size: 178 331 243 289 150 61 315 75 Fragments arranged by size: 461 333 331 315 289 254 243 178 178 162 150 130 75 61 32 18 BspEI T'CCGG_A Cuts at: 0 2528 3210 Size: 2528 682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 367 1033 1177 1996 3210 Size: 367 666 144 819 1214 Fragments arranged by size: 1214 819 666 367 144 BsrBI CCG'CTC Cuts at: 0 228 546 3210 Size: 228 318 2664 Fragments arranged by size: 2664 318 228 BsrDI GCAATG_nn' Cuts at: 0 653 732 888 1709 1991 2147 3210 Size: 653 79 156 821 282 156 1063 Fragments arranged by size: 1063 821 653 282 156 156 79 BsrFI r'CCGG_y Cuts at: 0 35 272 1459 3149 3210 Size: 35 237 1187 1690 61 Fragments arranged by size: 1690 1187 237 61 35 BsrGI T'GTAC_A Cuts at: 0 2864 3210 Size: 2864 346 BssSI C'ACGA_G Cuts at: 0 1120 3210 Size: 1120 2090 BstAPI GCAn_nnn'nTGC Cuts at: 0 2006 3210 Size: 2006 1204 BstXI CCAn_nnnn'nTGG Cuts at: 0 457 936 1908 2113 3180 3210 Size: 457 479 972 205 1067 30 Fragments arranged by size: 1067 972 479 457 205 30 BstYI r'GATC_y Cuts at: 0 16 416 890 2285 2634 3210 Size: 16 400 474 1395 349 576 Fragments arranged by size: 1395 576 474 400 349 16 Bsu36I CC'TnA_GG Cuts at: 0 1967 2659 3210 Size: 1967 692 551 Fragments arranged by size: 1967 692 551 BtgI C'CryG_G Cuts at: 0 988 1095 1559 2327 2374 3018 3194 3210 Size: 988 107 464 768 47 644 176 16 Fragments arranged by size: 988 768 644 464 176 107 47 16 BtsI GCAGTG_nn' Cuts at: 0 127 345 775 1540 1840 3210 Size: 127 218 430 765 300 1370 Fragments arranged by size: 1370 765 430 300 218 127 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 822 857 3210 Size: 822 35 2353 Fragments arranged by size: 2353 822 35 EaeI y'GGCC_r Cuts at: 0 23 562 618 874 906 1133 1178 1223 Size: 23 539 56 256 32 227 45 45 Cuts at: 1223 2330 3210 Size: 1107 880 Fragments arranged by size: 1107 880 539 256 227 56 45 45 32 23 EarI CTCTTCn'nnn_ Cuts at: 0 2404 2482 3210 Size: 2404 78 728 Fragments arranged by size: 2404 728 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2878 3210 Size: 2878 332 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 412 1264 1288 2956 3144 3210 Size: 344 68 852 24 1668 188 66 Fragments arranged by size: 1668 852 344 188 68 66 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 1303 2437 2854 3210 Size: 1303 1134 417 356 Fragments arranged by size: 1303 1134 417 356 EcoO109I rG'GnC_Cy Cuts at: 0 206 207 379 497 921 1391 1658 1912 Size: 206 1 172 118 424 470 267 254 Cuts at: 1912 2028 2073 2074 3132 3210 Size: 116 45 1 1058 78 Fragments arranged by size: 1058 470 424 267 254 206 172 118 116 78 45 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 964 2385 3210 Size: 964 1421 825 Fragments arranged by size: 1421 964 825 FspI TGC'GCA Cuts at: 0 1399 3210 Size: 1399 1811 HaeII r_GCGC'y Cuts at: 0 141 148 2187 3126 3179 3210 Size: 141 7 2039 939 53 31 Fragments arranged by size: 2039 939 141 53 31 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1193 1225 2746 2778 3139 3171 3210 Size: 1193 32 1521 32 361 32 39 Fragments arranged by size: 1521 1193 361 39 32 32 32 HincII GTy'rAC Cuts at: 0 1212 3210 Size: 1212 1998 HindIII A'AGCT_T Cuts at: 0 666 3210 Size: 666 2544 KasI G'GCGC_C Cuts at: 0 137 3210 Size: 137 3073 KpnI G_GTAC'C Cuts at: 0 83 1437 3210 Size: 83 1354 1773 Fragments arranged by size: 1773 1354 83 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1742 2048 2519 2789 3210 Size: 1742 306 471 270 421 Fragments arranged by size: 1742 471 421 306 270 MscI TGG'CCA Cuts at: 0 620 908 1135 1225 3210 Size: 620 288 227 90 1985 Fragments arranged by size: 1985 620 288 227 90 MslI CAynn'nnrTG Cuts at: 0 455 1219 1533 2152 3210 Size: 455 764 314 619 1058 Fragments arranged by size: 1058 764 619 455 314 MspA1I CmG'CkG Cuts at: 0 69 265 568 899 1003 1197 1266 1863 Size: 69 196 303 331 104 194 69 597 Cuts at: 1863 2006 2134 2329 2520 2907 3210 Size: 143 128 195 191 387 303 Fragments arranged by size: 597 387 331 303 303 196 195 194 191 143 128 104 69 69 NaeI GCC'GGC Cuts at: 0 1461 3210 Size: 1461 1749 NarI GG'CG_CC Cuts at: 0 138 3210 Size: 138 3072 NcoI C'CATG_G Cuts at: 0 1095 3018 3210 Size: 1095 1923 192 Fragments arranged by size: 1923 1095 192 NgoMIV G'CCGG_C Cuts at: 0 1459 3210 Size: 1459 1751 NspI r_CATG'y Cuts at: 0 187 975 3210 Size: 187 788 2235 Fragments arranged by size: 2235 788 187 PasI CC'CwG_GG Cuts at: 0 240 1845 2745 2844 3180 3210 Size: 240 1605 900 99 336 30 Fragments arranged by size: 1605 900 336 240 99 30 PciI A'CATG_T Cuts at: 0 183 3210 Size: 183 3027 PflMI CCAn_nnn'nTGG Cuts at: 0 303 699 1061 1125 1494 1959 2616 3210 Size: 303 396 362 64 369 465 657 594 Fragments arranged by size: 657 594 465 396 369 362 303 64 PfoI T'CCnGG_A Cuts at: 0 215 935 1892 2933 3210 Size: 215 720 957 1041 277 Fragments arranged by size: 1041 957 720 277 215 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 712 744 3210 Size: 712 32 2466 Fragments arranged by size: 2466 712 32 PpuMI rG'GwC_Cy Cuts at: 0 379 497 1391 1658 1912 3210 Size: 379 118 894 267 254 1298 Fragments arranged by size: 1298 894 379 267 254 118 PshAI GACnn'nnGTC Cuts at: 0 433 868 3210 Size: 433 435 2342 Fragments arranged by size: 2342 435 433 PspOMI G'GGCC_C Cuts at: 0 174 206 921 2073 3131 3210 Size: 174 32 715 1152 1058 79 Fragments arranged by size: 1152 1058 715 174 79 32 PstI C_TGCA'G Cuts at: 0 134 281 770 1835 2207 2390 2498 3210 Size: 134 147 489 1065 372 183 108 712 Fragments arranged by size: 1065 712 489 372 183 147 134 108 PvuII CAG'CTG Cuts at: 0 265 568 1003 1266 2006 2520 3210 Size: 265 303 435 263 740 514 690 Fragments arranged by size: 740 690 514 435 303 265 263 SacII CC_GC'GG Cuts at: 0 2330 3210 Size: 2330 880 SanDI GG'GwC_CC Cuts at: 0 497 1658 1912 3210 Size: 497 1161 254 1298 Fragments arranged by size: 1298 1161 497 254 SapI GCTCTTCn'nnn_ Cuts at: 0 2482 3210 Size: 2482 728 ScaI AGT'ACT Cuts at: 0 2878 3044 3210 Size: 2878 166 166 Fragments arranged by size: 2878 166 166 SfcI C'TryA_G Cuts at: 0 130 277 766 1322 1831 2203 2386 2494 Size: 130 147 489 556 509 372 183 108 Cuts at: 2494 3210 Size: 716 Fragments arranged by size: 716 556 509 489 372 183 147 130 108 SfoI GGC'GCC Cuts at: 0 139 3210 Size: 139 3071 SmaI CCC'GGG Cuts at: 0 1675 3210 Size: 1675 1535 SmlI C'TyrA_G Cuts at: 0 648 1504 1682 2647 3210 Size: 648 856 178 965 563 Fragments arranged by size: 965 856 648 563 178 SspI AAT'ATT Cuts at: 0 1140 3210 Size: 1140 2070 StuI AGG'CCT Cuts at: 0 2896 3210 Size: 2896 314 StyI C'CwwG_G Cuts at: 0 1037 1095 3018 3143 3210 Size: 1037 58 1923 125 67 Fragments arranged by size: 1923 1037 125 67 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 942 1108 1629 2116 2749 2889 3210 Size: 942 166 521 487 633 140 321 Fragments arranged by size: 942 633 521 487 321 166 140 TatI w'GTAC_w Cuts at: 0 188 2864 2876 3042 3210 Size: 188 2676 12 166 168 Fragments arranged by size: 2676 188 168 166 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 917 949 3210 Size: 917 32 2261 Fragments arranged by size: 2261 917 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 613 681 2400 2718 3210 Size: 613 68 1719 318 492 Fragments arranged by size: 1719 613 492 318 68 XhoI C'TCGA_G Cuts at: 0 1682 3210 Size: 1682 1528 XmaI C'CCGG_G Cuts at: 0 1673 3210 Size: 1673 1537 Enzymes that do cut: AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI AloI AlwNI ApaI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacII SanDI SapI ScaI SfcI SfoI SmaI SmlI SspI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AatII AclI AflII AgeI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BcgI BciVI BclI BfrBI BglII BmrI BmtI BplI BsaAI BsgI BsiEI BsmBI BspHI BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspAI HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PmeI PmlI PsiI PspXI PsrI PvuI RsrII SacI SalI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I XbaI XmnI ZraI