Restriction map for
pFN21AE3357
(Linear) (Six-base) MAPSORT of: KIEE3357.seq Check: 8643 from: 1 to: 3165
KIEE3357.orf
With 182 enzymes: SgfI *
July 3, 2012 17:45 ..
AccI GT'mk_AC
Cuts at: 0 2426 3165
Size: 2426 739
Acc65I G'GTAC_C
Cuts at: 0 700 3165
Size: 700 2465
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 866 958 1339 1478 2588 3165
Size: 289 577 92 381 139 1110 577
Fragments arranged by size:
1110 577 577 381 289 139 92
AflII C'TTAA_G
Cuts at: 0 745 3165
Size: 745 2420
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2984 3016 3165
Size: 2984 32 149
Fragments arranged by size:
2984 149 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1528 3165
Size: 1528 1637
ApoI r'AATT_y
Cuts at: 0 574 651 740 937 1056 1132 1163 1180
Size: 574 77 89 197 119 76 31 17
Cuts at: 1180 1369 2496 2617 2658 3165
Size: 189 1127 121 41 507
Fragments arranged by size:
1127 574 507 197 189 121 119 89
77 76 41 31 17
AseI AT'TA_AT
Cuts at: 0 89 1735 3165
Size: 89 1646 1430
Fragments arranged by size:
1646 1430 89
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 228 254 261 287 3165
Size: 228 26 7 26 2878
Fragments arranged by size:
2878 228 26 26 7
BanI G'GyrC_C
Cuts at: 0 700 1605 2445 3165
Size: 700 905 840 720
Fragments arranged by size:
905 840 720 700
BanII G_rGCy'C
Cuts at: 0 1488 3165
Size: 1488 1677
BbsI GAAGACnn'nnnn_
Cuts at: 0 1158 2078 2387 3165
Size: 1158 920 309 778
Fragments arranged by size:
1158 920 778 309
BbvCI CC'TCA_GC
Cuts at: 0 185 673 3165
Size: 185 488 2492
Fragments arranged by size:
2492 488 185
BciVI GTATCCnnnnn_n'
Cuts at: 0 1571 3165
Size: 1571 1594
BclI T'GATC_A
Cuts at: 0 1839 3165
Size: 1839 1326
BfrBI ATG'CAT
Cuts at: 0 1363 1599 3165
Size: 1363 236 1566
Fragments arranged by size:
1566 1363 236
BglII A'GATC_T
Cuts at: 0 993 3165
Size: 993 2172
Bme1580I G_kGCm'C
Cuts at: 0 1820 3165
Size: 1820 1345
BmrI ACTGGGnnnn_n'
Cuts at: 0 1833 2021 3165
Size: 1833 188 1144
Fragments arranged by size:
1833 1144 188
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 479 3165
Size: 479 2686
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1568 1683 2855 3165
Size: 305 1263 115 1172 310
Fragments arranged by size:
1263 1172 310 305 115
Bpu10I CC'TnA_GC
Cuts at: 0 185 673 3165
Size: 185 488 2492
Fragments arranged by size:
2492 488 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 602 1362 2525 3165
Size: 342 260 760 1163 640
Fragments arranged by size:
1163 760 640 342 260
BsaI GGTCTCn'nnnn_
Cuts at: 0 2201 3165
Size: 2201 964
BsaWI w'CCGG_w
Cuts at: 0 1321 2046 3165
Size: 1321 725 1119
Fragments arranged by size:
1321 1119 725
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2984 3014 3165
Size: 2984 30 151
Fragments arranged by size:
2984 151 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 865 2008 2608 3165
Size: 208 657 1143 600 557
Fragments arranged by size:
1143 657 600 557 208
BseYI C'CCAG_C
Cuts at: 0 1813 3165
Size: 1813 1352
BsmI GAATG_Cn'
Cuts at: 0 177 346 3165
Size: 177 169 2819
Fragments arranged by size:
2819 177 169
BsmBI CGTCTCn'nnnn_
Cuts at: 0 847 2159 3165
Size: 847 1312 1006
Fragments arranged by size:
1312 1006 847
Bsp1286I G_dGCh'C
Cuts at: 0 1488 1820 3165
Size: 1488 332 1345
Fragments arranged by size:
1488 1345 332
BspHI T'CATG_A
Cuts at: 0 2870 3165
Size: 2870 295
BsrDI GCAATG_nn'
Cuts at: 0 234 1025 3165
Size: 234 791 2140
Fragments arranged by size:
2140 791 234
BsrGI T'GTAC_A
Cuts at: 0 1958 3165
Size: 1958 1207
BstAPI GCAn_nnn'nTGC
Cuts at: 0 18 2119 3165
Size: 18 2101 1046
Fragments arranged by size:
2101 1046 18
BstBI TT'CG_AA
Cuts at: 0 2828 3165
Size: 2828 337
BstYI r'GATC_y
Cuts at: 0 993 3165
Size: 993 2172
BtgI C'CryG_G
Cuts at: 0 5 881 3165
Size: 5 876 2284
Fragments arranged by size:
2284 876 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1642 3165
Size: 1642 1523
BtsI GCAGTG_nn'
Cuts at: 0 155 3165
Size: 155 3010
ClaI AT'CG_AT
Cuts at: 0 1773 2961 3165
Size: 1773 1188 204
Fragments arranged by size:
1773 1188 204
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2033 2068 3165
Size: 2033 35 1097
Fragments arranged by size:
2033 1097 35
DraIII CAC_nnn'GTG
Cuts at: 0 1435 3165
Size: 1435 1730
EaeI y'GGCC_r
Cuts at: 0 329 3165
Size: 329 2836
EarI CTCTTCn'nnn_
Cuts at: 0 427 842 857 1244 1895 2778 2830 3165
Size: 427 415 15 387 651 883 52 335
Fragments arranged by size:
883 651 427 415 387 335 52 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 305 866 958 1339 1478 1568 1683
Size: 289 16 561 92 381 139 90 115
Cuts at: 1683 2588 2855 3165
Size: 905 267 310
Fragments arranged by size:
905 561 381 310 289 267 139 115
92 90 16
EcoRI G'AATT_C
Cuts at: 0 1056 3165
Size: 1056 2109
EcoRV GAT'ATC
Cuts at: 0 2771 3165
Size: 2771 394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 319 3165
Size: 319 2846
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2215 2247 3165
Size: 2215 32 918
Fragments arranged by size:
2215 918 32
HincII GTy'rAC
Cuts at: 0 1109 1407 3165
Size: 1109 298 1758
Fragments arranged by size:
1758 1109 298
HindIII A'AGCT_T
Cuts at: 0 2129 2143 3165
Size: 2129 14 1022
Fragments arranged by size:
2129 1022 14
HpaI GTT'AAC
Cuts at: 0 1109 3165
Size: 1109 2056
KpnI G_GTAC'C
Cuts at: 0 704 3165
Size: 704 2461
MfeI C'AATT_G
Cuts at: 0 314 1727 3165
Size: 314 1413 1438
Fragments arranged by size:
1438 1413 314
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2327 3165
Size: 2327 838
MslI CAynn'nnrTG
Cuts at: 0 244 952 2300 3165
Size: 244 708 1348 865
Fragments arranged by size:
1348 865 708 244
NcoI C'CATG_G
Cuts at: 0 881 3165
Size: 881 2284
NsiI A_TGCA'T
Cuts at: 0 1365 1601 3165
Size: 1365 236 1564
Fragments arranged by size:
1564 1365 236
NspI r_CATG'y
Cuts at: 0 428 1215 1783 3165
Size: 428 787 568 1382
Fragments arranged by size:
1382 787 568 428
PflMI CCAn_nnn'nTGG
Cuts at: 0 1435 3165
Size: 1435 1730
PfoI T'CCnGG_A
Cuts at: 0 1573 3165
Size: 1573 1592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2984 3016 3165
Size: 2984 32 149
Fragments arranged by size:
2984 149 32
PstI C_TGCA'G
Cuts at: 0 26 3165
Size: 26 3139
SapI GCTCTTCn'nnn_
Cuts at: 0 1895 3165
Size: 1895 1270
ScaI AGT'ACT
Cuts at: 0 923 3165
Size: 923 2242
SfcI C'TryA_G
Cuts at: 0 22 106 443 2942 3165
Size: 22 84 337 2499 223
Fragments arranged by size:
2499 337 223 84 22
SmlI C'TyrA_G
Cuts at: 0 321 617 745 1377 2540 3165
Size: 321 296 128 632 1163 625
Fragments arranged by size:
1163 632 625 321 296 128
StyI C'CwwG_G
Cuts at: 0 881 1340 1609 2285 2976 3165
Size: 881 459 269 676 691 189
Fragments arranged by size:
881 691 676 459 269 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 3165
Size: 112 3053
TatI w'GTAC_w
Cuts at: 0 921 1739 1865 1958 3165
Size: 921 818 126 93 1207
Fragments arranged by size:
1207 921 818 126 93
XbaI T'CTAG_A
Cuts at: 0 2220 3165
Size: 2220 945
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1810 3165
Size: 1810 1355
XmnI GAAnn'nnTTC
Cuts at: 0 1163 2270 2660 3165
Size: 1163 1107 390 505
Fragments arranged by size:
1163 1107 505 390
Enzymes that do cut:
AccI Acc65I AcuI AflII AloI AlwNI ApoI AseI
BaeI BanI BanII BbsI BbvCI BciVI BclI BfrBI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsmI BsmBI Bsp1286I BspHI
BsrDI BsrGI BstAPI BstBI BstYI BtgI BtgZI BtsI
ClaI CspCI DraIII EaeI EarI Eco57MI EcoRI EcoRV
FalI Hin4I HincII HindIII HpaI KpnI MfeI MmeI
MslI NcoI NsiI NspI PflMI PfoI PpiI PstI
SapI ScaI SfcI SmlI StyI TaqII TatI XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
AlfI ApaI ApaLI AscI AsiSI AvaI AvrII BamHI
BbeI BcgI BglI BlpI BmgBI BmtI BsaAI BsaBI
BsaHI BsgI BsiEI BsiHKAI BsiWI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstEII BstXI BstZ17I Bsu36I DraI
DrdI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI
FspAI HaeII KasI MluI MscI MspA1I NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XhoI
XmaI ZraI