Restriction map for
pFN21AE3349
(Linear) (Six-base) MAPSORT of: KIEE3349.seq Check: 4245 from: 1 to: 3102
KIEE3349.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AccI GT'mk_AC
Cuts at: 0 383 3102
Size: 383 2719
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 1972 3102
Size: 174 1798 1130
Fragments arranged by size:
1798 1130 174
AflIII A'CryG_T
Cuts at: 0 966 3102
Size: 966 2136
AhdI GACnn_n'nnGTC
Cuts at: 0 516 3102
Size: 516 2586
AleI CACnn'nnGTG
Cuts at: 0 1740 3102
Size: 1740 1362
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 375 2632 3102
Size: 375 2257 470
Fragments arranged by size:
2257 470 375
AlwNI CAG_nnn'CTG
Cuts at: 0 1692 2007 2693 2789 3102
Size: 1692 315 686 96 313
Fragments arranged by size:
1692 686 315 313 96
ApaI G_GGCC'C
Cuts at: 0 172 1605 2635 3102
Size: 172 1433 1030 467
Fragments arranged by size:
1433 1030 467 172
ApaLI G'TGCA_C
Cuts at: 0 694 3102
Size: 694 2408
ApoI r'AATT_y
Cuts at: 0 2740 2977 3102
Size: 2740 237 125
Fragments arranged by size:
2740 237 125
AvaI C'yCGr_G
Cuts at: 0 2583 2915 3102
Size: 2583 332 187
Fragments arranged by size:
2583 332 187
BamHI G'GATC_C
Cuts at: 0 1681 3102
Size: 1681 1421
BanI G'GyrC_C
Cuts at: 0 21 503 1657 3102
Size: 21 482 1154 1445
Fragments arranged by size:
1445 1154 482 21
BanII G_rGCy'C
Cuts at: 0 172 1162 1605 2015 2135 2635 3102
Size: 172 990 443 410 120 500 467
Fragments arranged by size:
990 500 467 443 410 172 120
BbsI GAAGACnn'nnnn_
Cuts at: 0 1517 1820 3102
Size: 1517 303 1282
Fragments arranged by size:
1517 1282 303
BbvCI CC'TCA_GC
Cuts at: 0 1723 2549 3102
Size: 1723 826 553
Fragments arranged by size:
1723 826 553
BclI T'GATC_A
Cuts at: 0 267 3102
Size: 267 2835
BfrBI ATG'CAT
Cuts at: 0 2072 3102
Size: 2072 1030
BglII A'GATC_T
Cuts at: 0 1133 2909 3102
Size: 1133 1776 193
Fragments arranged by size:
1776 1133 193
Bme1580I G_kGCm'C
Cuts at: 0 172 698 1605 1660 2635 3102
Size: 172 526 907 55 975 467
Fragments arranged by size:
975 907 526 467 172 55
BmgBI CAC'GTC
Cuts at: 0 333 3090 3102
Size: 333 2757 12
Fragments arranged by size:
2757 333 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 2568 2793 3102
Size: 2568 225 309
Fragments arranged by size:
2568 309 225
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2508 3102
Size: 2508 594
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1632 1904 2029 2139 2424 2478 2924
Size: 191 1441 272 125 110 285 54 446
Cuts at: 2924 3102
Size: 178
Fragments arranged by size:
1441 446 285 272 191 178 125 110
54
Bpu10I CC'TnA_GC
Cuts at: 0 1723 2549 2719 3037 3102
Size: 1723 826 170 318 65
Fragments arranged by size:
1723 826 318 170 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 1778 2244 3102
Size: 1201 577 466 858
Fragments arranged by size:
1201 858 577 466
BsaI GGTCTCn'nnnn_
Cuts at: 0 2942 3102
Size: 2942 160
BsaBI GATnn'nnATC
Cuts at: 0 980 3102
Size: 980 2122
BsaHI Gr'CG_yC
Cuts at: 0 260 3102
Size: 260 2842
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 447 477 2779 2809 3102
Size: 447 30 2302 30 293
Fragments arranged by size:
2302 447 293 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 85 103 106 109 112 169 468
Size: 21 64 18 3 3 3 57 299
Cuts at: 468 1972 1975 2424 2427 3102
Size: 1504 3 449 3 675
Fragments arranged by size:
1504 675 449 299 64 57 21 18
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 565 1606 1624 1941 2000 3102
Size: 565 1041 18 317 59 1102
Fragments arranged by size:
1102 1041 565 317 59 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 678 3102
Size: 604 74 2424
Fragments arranged by size:
2424 604 74
BsiEI CG_ry'CG
Cuts at: 0 988 3102
Size: 988 2114
BsiHKAI G_wGCw'C
Cuts at: 0 698 1162 3102
Size: 698 464 1940
Fragments arranged by size:
1940 698 464
BsmBI CGTCTCn'nnnn_
Cuts at: 0 561 3102
Size: 561 2541
Bsp1286I G_dGCh'C
Cuts at: 0 172 698 1162 1605 1660 2015 2135 2635
Size: 172 526 464 443 55 355 120 500
Cuts at: 2635 3102
Size: 467
Fragments arranged by size:
526 500 467 464 443 355 172 120
55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2624 3102
Size: 2624 478
BsrDI GCAATG_nn'
Cuts at: 0 141 1730 2037 3102
Size: 141 1589 307 1065
Fragments arranged by size:
1589 1065 307 141
BsrFI r'CCGG_y
Cuts at: 0 2217 2528 3102
Size: 2217 311 574
Fragments arranged by size:
2217 574 311
BsrGI T'GTAC_A
Cuts at: 0 1357 3102
Size: 1357 1745
BssSI C'ACGA_G
Cuts at: 0 756 3102
Size: 756 2346
BstAPI GCAn_nnn'nTGC
Cuts at: 0 540 611 2036 3102
Size: 540 71 1425 1066
Fragments arranged by size:
1425 1066 540 71
BstEII G'GTnAC_C
Cuts at: 0 948 1221 3102
Size: 948 273 1881
Fragments arranged by size:
1881 948 273
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1779 2460 3102
Size: 1779 681 642
Fragments arranged by size:
1779 681 642
BstYI r'GATC_y
Cuts at: 0 1012 1133 1455 1681 1697 2909 3102
Size: 1012 121 322 226 16 1212 193
Fragments arranged by size:
1212 1012 322 226 193 121 16
Bsu36I CC'TnA_GG
Cuts at: 0 1501 3102
Size: 1501 1601
BtgI C'CryG_G
Cuts at: 0 2126 3102
Size: 2126 976
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2367 3102
Size: 2367 735
BtsI GCAGTG_nn'
Cuts at: 0 1280 2790 3102
Size: 1280 1510 312
Fragments arranged by size:
1510 1280 312
DrdI GACnn_nn'nnGTC
Cuts at: 0 679 2218 2874 3102
Size: 679 1539 656 228
Fragments arranged by size:
1539 679 656 228
EaeI y'GGCC_r
Cuts at: 0 985 2699 3102
Size: 985 1714 403
Fragments arranged by size:
1714 985 403
EagI C'GGCC_G
Cuts at: 0 985 3102
Size: 985 2117
EarI CTCTTCn'nnn_
Cuts at: 0 62 80 1856 2596 2954 3102
Size: 62 18 1776 740 358 148
Fragments arranged by size:
1776 740 358 148 62 18
EcoICRI GAG'CTC
Cuts at: 0 1160 3102
Size: 1160 1942
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 191 1632 1904 1972 2029 2139 2424
Size: 174 17 1441 272 68 57 110 285
Cuts at: 2424 2478 2924 3102
Size: 54 446 178
Fragments arranged by size:
1441 446 285 272 178 174 110 68
57 54 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1504 1954 2238 2439 3102
Size: 1504 450 284 201 663
Fragments arranged by size:
1504 663 450 284 201
EcoO109I rG'GnC_Cy
Cuts at: 0 1302 1601 1602 1945 2445 3102
Size: 1302 299 1 343 500 657
Fragments arranged by size:
1302 657 500 343 299 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2238 3102
Size: 2238 864
HaeII r_GCGC'y
Cuts at: 0 2090 2265 2481 3102
Size: 2090 175 216 621
Fragments arranged by size:
2090 621 216 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 259 261 291 502 534 1855 1887
Size: 229 30 2 30 211 32 1321 32
Cuts at: 1887 2002 2034 2779 2811 2956 2988 3102
Size: 115 32 745 32 145 32 114
Fragments arranged by size:
1321 745 229 211 145 115 114 32
32 32 32 32 30 30 2
MfeI C'AATT_G
Cuts at: 0 357 3102
Size: 357 2745
MluI A'CGCG_T
Cuts at: 0 966 3102
Size: 966 2136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 380 1091 2561 2980 3102
Size: 257 123 711 1470 419 122
Fragments arranged by size:
1470 711 419 257 123 122
MscI TGG'CCA
Cuts at: 0 2701 3102
Size: 2701 401
MslI CAynn'nnrTG
Cuts at: 0 701 1192 1740 3102
Size: 701 491 548 1362
Fragments arranged by size:
1362 701 548 491
MspA1I CmG'CkG
Cuts at: 0 60 107 379 1254 1610 1647 3102
Size: 60 47 272 875 356 37 1455
Fragments arranged by size:
1455 875 356 272 60 47 37
NaeI GCC'GGC
Cuts at: 0 2530 3102
Size: 2530 572
NgoMIV G'CCGG_C
Cuts at: 0 2528 3102
Size: 2528 574
NsiI A_TGCA'T
Cuts at: 0 2074 3102
Size: 2074 1028
NspI r_CATG'y
Cuts at: 0 3022 3102
Size: 3022 80
PflMI CCAn_nnn'nTGG
Cuts at: 0 795 1197 2007 3102
Size: 795 402 810 1095
Fragments arranged by size:
1095 810 795 402
PfoI T'CCnGG_A
Cuts at: 0 207 788 3102
Size: 207 581 2314
Fragments arranged by size:
2314 581 207
PpuMI rG'GwC_Cy
Cuts at: 0 1945 2445 3102
Size: 1945 500 657
Fragments arranged by size:
1945 657 500
PshAI GACnn'nnGTC
Cuts at: 0 379 3102
Size: 379 2723
PspOMI G'GGCC_C
Cuts at: 0 168 1601 2631 3102
Size: 168 1433 1030 471
Fragments arranged by size:
1433 1030 471 168
PspXI vC'TCGA_Gb
Cuts at: 0 2583 3102
Size: 2583 519
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2799 2831 3102
Size: 2799 32 271
Fragments arranged by size:
2799 271 32
PstI C_TGCA'G
Cuts at: 0 128 533 1697 2096 3102
Size: 128 405 1164 399 1006
Fragments arranged by size:
1164 1006 405 399 128
PvuII CAG'CTG
Cuts at: 0 60 1254 1610 1647 3102
Size: 60 1194 356 37 1455
Fragments arranged by size:
1455 1194 356 60 37
RsrII CG'GwC_CG
Cuts at: 0 551 3102
Size: 551 2551
SacI G_AGCT'C
Cuts at: 0 1162 3102
Size: 1162 1940
SanDI GG'GwC_CC
Cuts at: 0 1945 2445 3102
Size: 1945 500 657
Fragments arranged by size:
1945 657 500
SfcI C'TryA_G
Cuts at: 0 124 529 1693 2092 3102
Size: 124 405 1164 399 1010
Fragments arranged by size:
1164 1010 405 399 124
SmaI CCC'GGG
Cuts at: 0 2917 3102
Size: 2917 185
SmlI C'TyrA_G
Cuts at: 0 1216 1793 2223 2583 3102
Size: 1216 577 430 360 519
Fragments arranged by size:
1216 577 519 430 360
StuI AGG'CCT
Cuts at: 0 419 1120 1867 3102
Size: 419 701 747 1235
Fragments arranged by size:
1235 747 701 419
StyI C'CwwG_G
Cuts at: 0 426 3102
Size: 426 2676
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 39 539 1204 1951 2024 3102
Size: 39 500 665 747 73 1078
Fragments arranged by size:
1078 747 665 500 73 39
TatI w'GTAC_w
Cuts at: 0 1357 3102
Size: 1357 1745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 447 479 3102
Size: 447 32 2623
Fragments arranged by size:
2623 447 32
Tth111I GACn'n_nGTC
Cuts at: 0 2304 2392 3102
Size: 2304 88 710
Fragments arranged by size:
2304 710 88
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1194 3102
Size: 1194 1908
XhoI C'TCGA_G
Cuts at: 0 2583 3102
Size: 2583 519
XmaI C'CCGG_G
Cuts at: 0 2915 3102
Size: 2915 187
XmnI GAAnn'nnTTC
Cuts at: 0 2811 3102
Size: 2811 291
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BclI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I MfeI MluI MmeI
MscI MslI MspA1I NaeI NgoMIV NsiI NspI PflMI
PfoI PpuMI PshAI PspOMI PspXI PsrI PstI PvuII
RsrII SacI SanDI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AloI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BglI BlpI BmtI BsaAI BsaWI BsiWI BsmI BspEI
BspHI BsrBI BssHII BstBI BstZ17I ClaI CspCI DraI
DraIII EciI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI NarI NcoI NdeI NheI
NotI NruI PacI PasI PciI PmeI PmlI PpiI
PsiI PvuI SacII SalI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI ZraI