Restriction map for
pFN21AE3348
(Linear) (Six-base) MAPSORT of: KIEE3348.seq Check: 7563 from: 1 to: 3096
KIEE3348.orf
With 182 enzymes: SgfI *
December 5, 2014 12:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 263 1499 1560 1727 3096
Size: 263 1236 61 167 1369
Fragments arranged by size:
1369 1236 263 167 61
AatII G_ACGT'C
Cuts at: 0 2894 3096
Size: 2894 202
Acc65I G'GTAC_C
Cuts at: 0 79 3096
Size: 79 3017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 822 1117 1215 1380 1409 1686 1797
Size: 169 653 295 98 165 29 277 111
Cuts at: 1797 2086 3096
Size: 289 1010
Fragments arranged by size:
1010 653 295 289 277 169 165 111
98 29
AgeI A'CCGG_T
Cuts at: 0 1561 3096
Size: 1561 1535
AhdI GACnn_n'nnGTC
Cuts at: 0 1187 3096
Size: 1187 1909
AleI CACnn'nnGTG
Cuts at: 0 637 2513 3096
Size: 637 1876 583
Fragments arranged by size:
1876 637 583
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 3096
Size: 37 3059
AlwNI CAG_nnn'CTG
Cuts at: 0 561 1185 1500 1551 1971 2415 2871 3014
Size: 561 624 315 51 420 444 456 143
Cuts at: 3014 3096
Size: 82
Fragments arranged by size:
624 561 456 444 420 315 143 82
51
ApaI G_GGCC'C
Cuts at: 0 1806 2240 3021 3096
Size: 1806 434 781 75
Fragments arranged by size:
1806 781 434 75
AvaI C'yCGr_G
Cuts at: 0 573 3096
Size: 573 2523
BanI G'GyrC_C
Cuts at: 0 79 577 639 705 2098 3096
Size: 79 498 62 66 1393 998
Fragments arranged by size:
1393 998 498 79 66 62
BanII G_rGCy'C
Cuts at: 0 110 347 1806 2189 2240 2917 3021 3096
Size: 110 237 1459 383 51 677 104 75
Fragments arranged by size:
1459 677 383 237 110 104 75 51
BbeI G_GCGC'C
Cuts at: 0 709 3096
Size: 709 2387
BbsI GAAGACnn'nnnn_
Cuts at: 0 1397 2616 3096
Size: 1397 1219 480
Fragments arranged by size:
1397 1219 480
BbvCI CC'TCA_GC
Cuts at: 0 307 448 1654 1738 2395 3096
Size: 307 141 1206 84 657 701
Fragments arranged by size:
1206 701 657 307 141 84
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2124 2158 2235 2269 2874 2908 3096
Size: 2124 34 77 34 605 34 188
Fragments arranged by size:
2124 605 188 77 34 34 34
BfrBI ATG'CAT
Cuts at: 0 968 3096
Size: 968 2128
BglI GCCn_nnn'nGGC
Cuts at: 0 491 2299 2921 3096
Size: 491 1808 622 175
Fragments arranged by size:
1808 622 491 175
Bme1580I G_kGCm'C
Cuts at: 0 161 368 582 644 805 1806 2240 2253
Size: 161 207 214 62 161 1001 434 13
Cuts at: 2253 2345 2498 2709 3021 3096
Size: 92 153 211 312 75
Fragments arranged by size:
1001 434 312 214 211 207 161 161
153 92 75 62 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 1363 1539 1837 2664 2990 3096
Size: 1363 176 298 827 326 106
Fragments arranged by size:
1363 827 326 298 176 106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2457 3096
Size: 2457 639
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 583 685 1162 1660 2344 2371 2704
Size: 94 489 102 477 498 684 27 333
Cuts at: 2704 2743 2896 3096
Size: 39 153 200
Fragments arranged by size:
684 498 489 477 333 200 153 102
94 39 27
Bpu10I CC'TnA_GC
Cuts at: 0 307 388 448 847 1507 1654 1738 1879
Size: 307 81 60 399 660 147 84 141
Cuts at: 1879 2194 2395 2911 3096
Size: 315 201 516 185
Fragments arranged by size:
660 516 399 315 307 201 185 147
141 84 81 60
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 601 2194 3096
Size: 265 336 1593 902
Fragments arranged by size:
1593 902 336 265
BsaI GGTCTCn'nnnn_
Cuts at: 0 1135 1844 2839 3096
Size: 1135 709 995 257
Fragments arranged by size:
1135 995 709 257
BsaAI yAC'GTr
Cuts at: 0 1257 2940 3096
Size: 1257 1683 156
Fragments arranged by size:
1683 1257 156
BsaHI Gr'CG_yC
Cuts at: 0 706 2891 3096
Size: 706 2185 205
Fragments arranged by size:
2185 706 205
BsaWI w'CCGG_w
Cuts at: 0 1561 2108 3065 3096
Size: 1561 547 957 31
Fragments arranged by size:
1561 957 547 31
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2701 2968 3096
Size: 2701 267 128
Fragments arranged by size:
2701 267 128
BseYI C'CCAG_C
Cuts at: 0 259 346 818 918 1217 1381 1478 1775
Size: 259 87 472 100 299 164 97 297
Cuts at: 1775 2353 2519 2702 2861 3020 3024 3096
Size: 578 166 183 159 159 4 72
Fragments arranged by size:
578 472 299 297 259 183 166 164
159 159 100 97 87 72 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 64 1480 1846 1903 2269 2350 3096
Size: 7 57 1416 366 57 366 81 746
Fragments arranged by size:
1416 746 366 366 81 57 57 7
BsiEI CG_ry'CG
Cuts at: 0 2925 3092 3096
Size: 2925 167 4
Fragments arranged by size:
2925 167 4
BsiHKAI G_wGCw'C
Cuts at: 0 110 746 1616 2189 3096
Size: 110 636 870 573 907
Fragments arranged by size:
907 870 636 573 110
BsmI GAATG_Cn'
Cuts at: 0 903 3096
Size: 903 2193
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1981 3096
Size: 1981 1115
Bsp1286I G_dGCh'C
Cuts at: 0 110 161 347 368 582 644 746 805
Size: 110 51 186 21 214 62 102 59
Cuts at: 805 1616 1806 2189 2240 2253 2345 2498 2709
Size: 811 190 383 51 13 92 153 211
Cuts at: 2709 2917 3021 3096
Size: 208 104 75
Fragments arranged by size:
811 383 214 211 208 190 186 153
110 104 102 92 75 62 59 51
51 21 13
BspEI T'CCGG_A
Cuts at: 0 2108 3096
Size: 2108 988
BspHI T'CATG_A
Cuts at: 0 404 3096
Size: 404 2692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 659 1499 1560 1727 2423 3096
Size: 263 396 840 61 167 696 673
Fragments arranged by size:
840 696 673 396 263 167 61
BsrBI CCG'CTC
Cuts at: 0 1130 1883 2930 3096
Size: 1130 753 1047 166
Fragments arranged by size:
1130 1047 753 166
BsrDI GCAATG_nn'
Cuts at: 0 195 1827 3096
Size: 195 1632 1269
Fragments arranged by size:
1632 1269 195
BsrFI r'CCGG_y
Cuts at: 0 297 490 1561 3096
Size: 297 193 1071 1535
Fragments arranged by size:
1535 1071 297 193
BsrGI T'GTAC_A
Cuts at: 0 2660 3096
Size: 2660 436
BssHII G'CGCG_C
Cuts at: 0 2841 3096
Size: 2841 255
BssSI C'ACGA_G
Cuts at: 0 1609 3096
Size: 1609 1487
BstAPI GCAn_nnn'nTGC
Cuts at: 0 504 800 1500 2871 3096
Size: 504 296 700 1371 225
Fragments arranged by size:
1371 700 504 296 225
BstEII G'GTnAC_C
Cuts at: 0 1452 3096
Size: 1452 1644
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 69 1675 2998 3096
Size: 57 12 1606 1323 98
Fragments arranged by size:
1606 1323 98 57 12
BstYI r'GATC_y
Cuts at: 0 1431 3096
Size: 1431 1665
Bsu36I CC'TnA_GG
Cuts at: 0 710 3096
Size: 710 2386
BtgI C'CryG_G
Cuts at: 0 117 170 183 912 1307 2694 3086 3096
Size: 117 53 13 729 395 1387 392 10
Fragments arranged by size:
1387 729 395 392 117 53 13 10
BtsI GCAGTG_nn'
Cuts at: 0 1501 2286 2651 3096
Size: 1501 785 365 445
Fragments arranged by size:
1501 785 445 365
DraIII CAC_nnn'GTG
Cuts at: 0 184 3096
Size: 184 2912
EaeI y'GGCC_r
Cuts at: 0 59 719 1153 2922 3089 3096
Size: 59 660 434 1769 167 7
Fragments arranged by size:
1769 660 434 167 59 7
EagI C'GGCC_G
Cuts at: 0 2922 3089 3096
Size: 2922 167 7
Fragments arranged by size:
2922 167 7
EarI CTCTTCn'nnn_
Cuts at: 0 371 3096
Size: 371 2725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 770 2701 3096
Size: 770 1931 395
Fragments arranged by size:
1931 770 395
EcoICRI GAG'CTC
Cuts at: 0 108 2187 3096
Size: 108 2079 909
Fragments arranged by size:
2079 909 108
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 169 583 685 822 1117 1162 1215
Size: 94 75 414 102 137 295 45 53
Cuts at: 1215 1380 1409 1660 1686 1797 2086 2344 2371
Size: 165 29 251 26 111 289 258 27
Cuts at: 2371 2704 2743 2896 3096
Size: 333 39 153 200
Fragments arranged by size:
414 333 295 289 258 251 200 165
153 137 111 102 94 75 53 45
39 29 27 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 43 2323 2431 2875 3096
Size: 43 2280 108 444 221
Fragments arranged by size:
2280 444 221 108 43
EcoO109I rG'GnC_Cy
Cuts at: 0 714 993 1235 1353 1644 1803 2070 2237
Size: 714 279 242 118 291 159 267 167
Cuts at: 2237 2391 2412 2436 2523 3082 3096
Size: 154 21 24 87 559 14
Fragments arranged by size:
714 559 291 279 267 242 167 159
154 118 87 24 21 14
FspI TGC'GCA
Cuts at: 0 1009 1723 1945 3096
Size: 1009 714 222 1151
Fragments arranged by size:
1151 1009 714 222
HaeII r_GCGC'y
Cuts at: 0 20 709 2201 2276 2703 2871 2958 3096
Size: 20 689 1492 75 427 168 87 138
Fragments arranged by size:
1492 689 427 168 138 87 75 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1173 1205 3096
Size: 1173 32 1891
Fragments arranged by size:
1891 1173 32
HindIII A'AGCT_T
Cuts at: 0 1012 3096
Size: 1012 2084
KasI G'GCGC_C
Cuts at: 0 705 3096
Size: 705 2391
KpnI G_GTAC'C
Cuts at: 0 83 3096
Size: 83 3013
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 1247 3096
Size: 239 1008 1849
Fragments arranged by size:
1849 1008 239
MscI TGG'CCA
Cuts at: 0 61 721 3096
Size: 61 660 2375
Fragments arranged by size:
2375 660 61
MslI CAynn'nnrTG
Cuts at: 0 233 409 466 637 2513 3096
Size: 233 176 57 171 1876 583
Fragments arranged by size:
1876 583 233 176 171 57
MspA1I CmG'CkG
Cuts at: 0 30 770 1005 1283 1557 1817 2064 2126
Size: 30 740 235 278 274 260 247 62
Cuts at: 2126 2682 3024 3096
Size: 556 342 72
Fragments arranged by size:
740 556 342 278 274 260 247 235
72 62 30
NaeI GCC'GGC
Cuts at: 0 492 3096
Size: 492 2604
NarI GG'CG_CC
Cuts at: 0 706 3096
Size: 706 2390
NcoI C'CATG_G
Cuts at: 0 170 1307 3096
Size: 170 1137 1789
Fragments arranged by size:
1789 1137 170
NdeI CA'TA_TG
Cuts at: 0 1835 3096
Size: 1835 1261
NgoMIV G'CCGG_C
Cuts at: 0 490 3096
Size: 490 2606
NsiI A_TGCA'T
Cuts at: 0 970 3096
Size: 970 2126
NspI r_CATG'y
Cuts at: 0 502 1113 1683 3096
Size: 502 611 570 1413
Fragments arranged by size:
1413 611 570 502
PasI CC'CwG_GG
Cuts at: 0 75 3096
Size: 75 3021
PflMI CCAn_nnn'nTGG
Cuts at: 0 123 3096
Size: 123 2973
PpuMI rG'GwC_Cy
Cuts at: 0 714 1353 2412 2523 3082 3096
Size: 714 639 1059 111 559 14
Fragments arranged by size:
1059 714 639 559 111 14
PspOMI G'GGCC_C
Cuts at: 0 1802 2236 3017 3096
Size: 1802 434 781 79
Fragments arranged by size:
1802 781 434 79
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 943 975 3096
Size: 943 32 2121
Fragments arranged by size:
2121 943 32
PstI C_TGCA'G
Cuts at: 0 553 1196 1346 1411 1496 1571 1789 3096
Size: 553 643 150 65 85 75 218 1307
Fragments arranged by size:
1307 643 553 218 150 85 75 65
PvuII CAG'CTG
Cuts at: 0 1005 2064 2126 2682 3024 3096
Size: 1005 1059 62 556 342 72
Fragments arranged by size:
1059 1005 556 342 72 62
RsrII CG'GwC_CG
Cuts at: 0 2820 3096
Size: 2820 276
SacI G_AGCT'C
Cuts at: 0 110 2189 3096
Size: 110 2079 907
Fragments arranged by size:
2079 907 110
SanDI GG'GwC_CC
Cuts at: 0 3082 3096
Size: 3082 14
SapI GCTCTTCn'nnn_
Cuts at: 0 371 3096
Size: 371 2725
SbfI CC_TGCA'GG
Cuts at: 0 553 3096
Size: 553 2543
SexAI A'CCwGG_T
Cuts at: 0 1448 3096
Size: 1448 1648
SfcI C'TryA_G
Cuts at: 0 549 1192 1342 1407 1492 1567 1785 1963
Size: 549 643 150 65 85 75 218 178
Cuts at: 1963 3096
Size: 1133
Fragments arranged by size:
1133 643 549 218 178 150 85 75
65
SfoI GGC'GCC
Cuts at: 0 707 3096
Size: 707 2389
SmaI CCC'GGG
Cuts at: 0 575 3096
Size: 575 2521
SmlI C'TyrA_G
Cuts at: 0 280 616 2209 3096
Size: 280 336 1593 887
Fragments arranged by size:
1593 887 336 280
SphI G_CATG'C
Cuts at: 0 502 1683 3096
Size: 502 1181 1413
Fragments arranged by size:
1413 1181 502
StuI AGG'CCT
Cuts at: 0 863 985 1877 3096
Size: 863 122 892 1219
Fragments arranged by size:
1219 892 863 122
StyI C'CwwG_G
Cuts at: 0 170 585 980 1307 1427 1872 2165 2171
Size: 170 415 395 327 120 445 293 6
Cuts at: 2171 3096
Size: 925
Fragments arranged by size:
925 445 415 395 327 293 170 120
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 619 2479 2646 2766 3096
Size: 619 1860 167 120 330
Fragments arranged by size:
1860 619 330 167 120
TatI w'GTAC_w
Cuts at: 0 620 2660 3096
Size: 620 2040 436
Fragments arranged by size:
2040 620 436
Tth111I GACn'n_nGTC
Cuts at: 0 2824 3096
Size: 2824 272
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 354 792 2230 2853 2995 3096
Size: 354 438 1438 623 142 101
Fragments arranged by size:
1438 623 438 354 142 101
XmaI C'CCGG_G
Cuts at: 0 573 3096
Size: 573 2523
ZraI GAC'GTC
Cuts at: 0 2892 3096
Size: 2892 204
Enzymes that do cut:
AarI AatII Acc65I AcuI AgeI AhdI AleI AlfI
AlwNI ApaI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BfrBI BglI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PflMI PpuMI PspOMI PsrI PstI PvuII RsrII SacI
SanDI SapI SbfI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BglII BlpI BmgBI BmtI BsaBI BsaXI BsiWI BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV
FalI FseI FspAI HincII HpaI MfeI MluI NheI
NotI NruI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI SacII SalI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI
XbaI XhoI XmnI