Restriction map for
pFN21AE3345
(Linear) (Six-base) MAPSORT of: KIEE3345.seq Check: 5990 from: 1 to: 3057
KIEE3345.orf
With 182 enzymes: SgfI *
March 22, 2012 16:30 ..
AccI GT'mk_AC
Cuts at: 0 1139 3057
Size: 1139 1918
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 290 569 738 1087 2372 3057
Size: 218 72 279 169 349 1285 685
Fragments arranged by size:
1285 685 349 279 218 169 72
AflII C'TTAA_G
Cuts at: 0 610 774 3057
Size: 610 164 2283
Fragments arranged by size:
2283 610 164
AflIII A'CryG_T
Cuts at: 0 1346 3057
Size: 1346 1711
AleI CACnn'nnGTG
Cuts at: 0 776 3057
Size: 776 2281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 648 3057
Size: 648 2409
AlwNI CAG_nnn'CTG
Cuts at: 0 915 1011 1033 1341 2523 3057
Size: 915 96 22 308 1182 534
Fragments arranged by size:
1182 915 534 308 96 22
ApaI G_GGCC'C
Cuts at: 0 147 3057
Size: 147 2910
ApoI r'AATT_y
Cuts at: 0 359 386 1467 1489 2042 2478 3057
Size: 359 27 1081 22 553 436 579
Fragments arranged by size:
1081 579 553 436 359 27 22
AvrII C'CTAG_G
Cuts at: 0 2437 3057
Size: 2437 620
BamHI G'GATC_C
Cuts at: 0 678 3057
Size: 678 2379
BanII G_rGCy'C
Cuts at: 0 147 2204 3057
Size: 147 2057 853
Fragments arranged by size:
2057 853 147
BbsI GAAGACnn'nnnn_
Cuts at: 0 1075 1640 1675 1906 3031 3057
Size: 1075 565 35 231 1125 26
Fragments arranged by size:
1125 1075 565 231 35 26
BclI T'GATC_A
Cuts at: 0 456 477 1713 3057
Size: 456 21 1236 1344
Fragments arranged by size:
1344 1236 456 21
BglI GCCn_nnn'nGGC
Cuts at: 0 768 3057
Size: 768 2289
BglII A'GATC_T
Cuts at: 0 2184 3057
Size: 2184 873
BlpI GC'TnA_GC
Cuts at: 0 2198 3057
Size: 2198 859
Bme1580I G_kGCm'C
Cuts at: 0 147 487 773 3057
Size: 147 340 286 2284
Fragments arranged by size:
2284 340 286 147
BmrI ACTGGGnnnn_n'
Cuts at: 0 465 1865 3057
Size: 465 1400 1192
Fragments arranged by size:
1400 1192 465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1717 2702 3057
Size: 1196 521 985 355
Fragments arranged by size:
1196 985 521 355
Bpu10I CC'TnA_GC
Cuts at: 0 569 1262 2102 3057
Size: 569 693 840 955
Fragments arranged by size:
955 840 693 569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2694 3057
Size: 2694 363
BsaI GGTCTCn'nnnn_
Cuts at: 0 2164 3057
Size: 2164 893
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 388 418 3057
Size: 388 30 2639
Fragments arranged by size:
2639 388 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1534 2839 3057
Size: 1534 1305 218
Fragments arranged by size:
1534 1305 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1543 3057
Size: 1543 1514
BsmI GAATG_Cn'
Cuts at: 0 2457 3057
Size: 2457 600
Bsp1286I G_dGCh'C
Cuts at: 0 147 487 773 2204 3057
Size: 147 340 286 1431 853
Fragments arranged by size:
1431 853 340 286 147
BspHI T'CATG_A
Cuts at: 0 173 2049 2501 3057
Size: 173 1876 452 556
Fragments arranged by size:
1876 556 452 173
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2718 3057
Size: 2718 339
BsrDI GCAATG_nn'
Cuts at: 0 1408 1646 1793 2530 3057
Size: 1408 238 147 737 527
Fragments arranged by size:
1408 737 527 238 147
BsrGI T'GTAC_A
Cuts at: 0 1343 3057
Size: 1343 1714
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2532 3057
Size: 2532 525
BstYI r'GATC_y
Cuts at: 0 678 2184 3057
Size: 678 1506 873
Fragments arranged by size:
1506 873 678
BtsI GCAGTG_nn'
Cuts at: 0 591 1445 3057
Size: 591 854 1612
Fragments arranged by size:
1612 854 591
ClaI AT'CG_AT
Cuts at: 0 369 3057
Size: 369 2688
DraI TTT'AAA
Cuts at: 0 1836 1859 2477 2495 2588 2900 3057
Size: 1836 23 618 18 93 312 157
Fragments arranged by size:
1836 618 312 157 93 23 18
DrdI GACnn_nn'nnGTC
Cuts at: 0 1147 3057
Size: 1147 1910
EarI CTCTTCn'nnn_
Cuts at: 0 24 596 962 1955 3057
Size: 24 572 366 993 1102
Fragments arranged by size:
1102 993 572 366 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 290 569 738 1087 1196 1717 2372
Size: 218 72 279 169 349 109 521 655
Cuts at: 2372 2702 3057
Size: 330 355
Fragments arranged by size:
655 521 355 349 330 279 218 169
109 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 756 2126 3057
Size: 756 1370 931
Fragments arranged by size:
1370 931 756
EcoRV GAT'ATC
Cuts at: 0 171 3057
Size: 171 2886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 625 789 3057
Size: 625 164 2268
Fragments arranged by size:
2268 625 164
FspI TGC'GCA
Cuts at: 0 2023 3057
Size: 2023 1034
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 3057
Size: 1369 32 1656
Fragments arranged by size:
1656 1369 32
HincII GTy'rAC
Cuts at: 0 688 749 1140 2292 3057
Size: 688 61 391 1152 765
Fragments arranged by size:
1152 765 688 391 61
HindIII A'AGCT_T
Cuts at: 0 1911 3057
Size: 1911 1146
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 1169 2589 3057
Size: 90 1079 1420 468
Fragments arranged by size:
1420 1079 468 90
MslI CAynn'nnrTG
Cuts at: 0 776 2091 2828 3057
Size: 776 1315 737 229
Fragments arranged by size:
1315 776 737 229
MspA1I CmG'CkG
Cuts at: 0 1030 2019 3057
Size: 1030 989 1038
Fragments arranged by size:
1038 1030 989
NspI r_CATG'y
Cuts at: 0 1138 1350 2472 3057
Size: 1138 212 1122 585
Fragments arranged by size:
1138 1122 585 212
PasI CC'CwG_GG
Cuts at: 0 2517 3057
Size: 2517 540
PciI A'CATG_T
Cuts at: 0 1346 3057
Size: 1346 1711
PsiI TTA'TAA
Cuts at: 0 829 971 3057
Size: 829 142 2086
Fragments arranged by size:
2086 829 142
PspOMI G'GGCC_C
Cuts at: 0 143 3057
Size: 143 2914
PstI C_TGCA'G
Cuts at: 0 404 1016 3057
Size: 404 612 2041
Fragments arranged by size:
2041 612 404
PvuII CAG'CTG
Cuts at: 0 1030 2019 3057
Size: 1030 989 1038
Fragments arranged by size:
1038 1030 989
SalI G'TCGA_C
Cuts at: 0 1138 3057
Size: 1138 1919
SapI GCTCTTCn'nnn_
Cuts at: 0 962 3057
Size: 962 2095
ScaI AGT'ACT
Cuts at: 0 299 1763 3057
Size: 299 1464 1294
Fragments arranged by size:
1464 1294 299
SfcI C'TryA_G
Cuts at: 0 400 1012 2853 3057
Size: 400 612 1841 204
Fragments arranged by size:
1841 612 400 204
SmlI C'TyrA_G
Cuts at: 0 610 774 2673 3057
Size: 610 164 1899 384
Fragments arranged by size:
1899 610 384 164
SpeI A'CTAG_T
Cuts at: 0 2077 3057
Size: 2077 980
SspI AAT'ATT
Cuts at: 0 1272 1305 1650 1855 3057
Size: 1272 33 345 205 1202
Fragments arranged by size:
1272 1202 345 205 33
StyI C'CwwG_G
Cuts at: 0 764 2303 2437 3057
Size: 764 1539 134 620
Fragments arranged by size:
1539 764 620 134
SwaI ATTT'AAAT
Cuts at: 0 1859 3057
Size: 1859 1198
TatI w'GTAC_w
Cuts at: 0 297 1343 1761 1803 1814 3057
Size: 297 1046 418 42 11 1243
Fragments arranged by size:
1243 1046 418 297 42 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1183 1215 1873 1905 3057
Size: 1183 32 658 32 1152
Fragments arranged by size:
1183 1152 658 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1674 3057
Size: 1674 1383
XbaI T'CTAG_A
Cuts at: 0 22 3057
Size: 22 3035
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 222 3057
Size: 222 2835
XmnI GAAnn'nnTTC
Cuts at: 0 2095 3057
Size: 2095 962
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlfI AlwNI ApaI
ApoI AvrII BamHI BanII BbsI BclI BglI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI
BseRI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstYI BtsI ClaI DraI DrdI EarI Eco57MI
EcoNI EcoRV FalI FspI Hin4I HincII HindIII MmeI
MslI MspA1I NspI PasI PciI PsiI PspOMI PstI
PvuII SalI SapI ScaI SfcI SmlI SpeI SspI
StyI SwaI TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AloI
ApaLI AscI AseI AsiSI AvaI BaeI BanI BbeI
BbvCI BcgI BciVI BfrBI BmgBI BmtI BplI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI CspCI DraIII EaeI EagI
EciI EcoICRI EcoO109I EcoRI FseI FspAI HaeII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspXI PsrI
PvuI RsrII SacI SacII SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
TaqII XhoI XmaI ZraI