(Linear) (Six-base) MAPSORT of: KIEE3344.seq Check: 1857 from: 1 to: 3012 KIEE3344.orf With 182 enzymes: SgfI * August 14, 2013 11:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1152 2076 3012 Size: 1152 924 936 Fragments arranged by size: 1152 936 924 AatII G_ACGT'C Cuts at: 0 296 2714 3012 Size: 296 2418 298 Fragments arranged by size: 2418 298 296 AccI GT'mk_AC Cuts at: 0 296 3012 Size: 296 2716 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 295 318 601 706 820 3012 Size: 250 45 23 283 105 114 2192 Fragments arranged by size: 2192 283 250 114 105 45 23 AhdI GACnn_n'nnGTC Cuts at: 0 771 3012 Size: 771 2241 AleI CACnn'nnGTG Cuts at: 0 2437 3012 Size: 2437 575 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 640 3012 Size: 640 2372 AlwNI CAG_nnn'CTG Cuts at: 0 465 696 750 870 900 987 1134 1290 Size: 465 231 54 120 30 87 147 156 Cuts at: 1290 1331 1449 1502 2001 2307 2729 2847 3012 Size: 41 118 53 499 306 422 118 165 Fragments arranged by size: 499 465 422 306 231 165 156 147 120 118 118 87 54 53 41 30 ApaLI G'TGCA_C Cuts at: 0 2265 3012 Size: 2265 747 AvaI C'yCGr_G Cuts at: 0 236 1527 1797 1830 2022 3012 Size: 236 1291 270 33 192 990 Fragments arranged by size: 1291 990 270 236 192 33 BamHI G'GATC_C Cuts at: 0 1691 3012 Size: 1691 1321 BanI G'GyrC_C Cuts at: 0 455 1834 2197 2439 2827 2886 3012 Size: 455 1379 363 242 388 59 126 Fragments arranged by size: 1379 455 388 363 242 126 59 BanII G_rGCy'C Cuts at: 0 1268 1536 1580 1804 1874 2223 2401 3007 Size: 1268 268 44 224 70 349 178 606 Cuts at: 3007 3012 Size: 5 Fragments arranged by size: 1268 606 349 268 224 178 70 44 5 BbeI G_GCGC'C Cuts at: 0 2890 3012 Size: 2890 122 BbsI GAAGACnn'nnnn_ Cuts at: 0 238 3012 Size: 238 2774 BbvCI CC'TCA_GC Cuts at: 0 574 3012 Size: 574 2438 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 478 512 3012 Size: 478 34 2500 Fragments arranged by size: 2500 478 34 BciVI GTATCCnnnnn_n' Cuts at: 0 2175 3012 Size: 2175 837 BfrBI ATG'CAT Cuts at: 0 1302 1732 3012 Size: 1302 430 1280 Fragments arranged by size: 1302 1280 430 BglI GCCn_nnn'nGGC Cuts at: 0 205 2014 3012 Size: 205 1809 998 Fragments arranged by size: 1809 998 205 BlpI GC'TnA_GC Cuts at: 0 595 2222 2944 3012 Size: 595 1627 722 68 Fragments arranged by size: 1627 722 595 68 Bme1580I G_kGCm'C Cuts at: 0 215 1837 2269 2444 2501 2830 3012 Size: 215 1622 432 175 57 329 182 Fragments arranged by size: 1622 432 329 215 182 175 57 BmgBI CAC'GTC Cuts at: 0 1385 2112 2772 3012 Size: 1385 727 660 240 Fragments arranged by size: 1385 727 660 240 BmrI ACTGGGnnnn_n' Cuts at: 0 975 3012 Size: 975 2037 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1164 3012 Size: 1164 1848 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 286 538 880 1401 1528 1759 1912 1927 Size: 286 252 342 521 127 231 153 15 Cuts at: 1927 1978 2023 2199 2385 2619 2869 2943 3012 Size: 51 45 176 186 234 250 74 69 Fragments arranged by size: 521 342 286 252 250 234 231 186 176 153 127 74 69 51 45 15 Bpu10I CC'TnA_GC Cuts at: 0 574 1547 1696 2521 3012 Size: 574 973 149 825 491 Fragments arranged by size: 973 825 574 491 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1225 2595 2788 3012 Size: 1225 1370 193 224 Fragments arranged by size: 1370 1225 224 193 BsaI GGTCTCn'nnnn_ Cuts at: 0 355 484 1076 1517 2867 3012 Size: 355 129 592 441 1350 145 Fragments arranged by size: 1350 592 441 355 145 129 BsaAI yAC'GTr Cuts at: 0 2408 3012 Size: 2408 604 BsaBI GATnn'nnATC Cuts at: 0 2749 3012 Size: 2749 263 BsaHI Gr'CG_yC Cuts at: 0 293 1661 2711 2887 3012 Size: 293 1368 1050 176 125 Fragments arranged by size: 1368 1050 293 176 125 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 40 70 661 691 2522 2552 3012 Size: 40 30 591 30 1831 30 460 Fragments arranged by size: 1831 591 460 40 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 175 178 424 685 799 820 1924 Size: 61 114 3 246 261 114 21 1104 Cuts at: 1924 2482 2530 2599 2851 2881 3012 Size: 558 48 69 252 30 131 Fragments arranged by size: 1104 558 261 252 246 131 114 114 69 61 48 30 21 3 BseYI C'CCAG_C Cuts at: 0 106 385 1435 2383 2651 3012 Size: 106 279 1050 948 268 361 Fragments arranged by size: 1050 948 361 279 268 106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 157 493 592 2092 2715 2722 3012 Size: 157 336 99 1500 623 7 290 Fragments arranged by size: 1500 623 336 290 157 99 7 BsiHKAI G_wGCw'C Cuts at: 0 476 1113 1241 1580 2269 2492 3012 Size: 476 637 128 339 689 223 520 Fragments arranged by size: 689 637 520 476 339 223 128 BsiWI C'GTAC_G Cuts at: 0 90 3012 Size: 90 2922 BsmI GAATG_Cn' Cuts at: 0 1047 3012 Size: 1047 1965 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1313 2778 3012 Size: 1313 1465 234 Fragments arranged by size: 1465 1313 234 Bsp1286I G_dGCh'C Cuts at: 0 215 476 1113 1241 1268 1536 1580 1804 Size: 215 261 637 128 27 268 44 224 Cuts at: 1804 1837 1874 2223 2269 2401 2444 2492 2501 Size: 33 37 349 46 132 43 48 9 Cuts at: 2501 2830 3007 3012 Size: 329 177 5 Fragments arranged by size: 637 349 329 268 261 224 215 177 132 128 48 46 44 43 37 33 27 9 5 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 134 1152 2076 3012 Size: 134 1018 924 936 Fragments arranged by size: 1018 936 924 134 BsrBI CCG'CTC Cuts at: 0 930 1415 1658 3012 Size: 930 485 243 1354 Fragments arranged by size: 1354 930 485 243 BsrDI GCAATG_nn' Cuts at: 0 624 1732 2091 3012 Size: 624 1108 359 921 Fragments arranged by size: 1108 921 624 359 BsrGI T'GTAC_A Cuts at: 0 1451 3012 Size: 1451 1561 BssSI C'ACGA_G Cuts at: 0 2494 3012 Size: 2494 518 BstAPI GCAn_nnn'nTGC Cuts at: 0 696 3012 Size: 696 2316 BstEII G'GTnAC_C Cuts at: 0 306 1704 1791 2655 3012 Size: 306 1398 87 864 357 Fragments arranged by size: 1398 864 357 306 87 BstXI CCAn_nnnn'nTGG Cuts at: 0 2391 3012 Size: 2391 621 BstYI r'GATC_y Cuts at: 0 1511 1691 2516 3012 Size: 1511 180 825 496 Fragments arranged by size: 1511 825 496 180 Bsu36I CC'TnA_GG Cuts at: 0 751 3012 Size: 751 2261 BtgI C'CryG_G Cuts at: 0 935 1273 1916 2057 3012 Size: 935 338 643 141 955 Fragments arranged by size: 955 935 643 338 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 240 2949 3012 Size: 240 2709 63 Fragments arranged by size: 2709 240 63 BtsI GCAGTG_nn' Cuts at: 0 2269 3012 Size: 2269 743 DraIII CAC_nnn'GTG Cuts at: 0 2160 3012 Size: 2160 852 EaeI y'GGCC_r Cuts at: 0 485 641 1463 2054 3012 Size: 485 156 822 591 958 Fragments arranged by size: 958 822 591 485 156 EarI CTCTTCn'nnn_ Cuts at: 0 965 997 3012 Size: 965 32 2015 Fragments arranged by size: 2015 965 32 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 859 1689 1825 2944 3012 Size: 859 830 136 1119 68 Fragments arranged by size: 1119 859 830 136 68 EcoICRI GAG'CTC Cuts at: 0 1578 3012 Size: 1578 1434 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 250 286 295 318 538 601 706 820 Size: 250 36 9 23 220 63 105 114 Cuts at: 820 880 1401 1528 1759 1912 1927 1978 2023 Size: 60 521 127 231 153 15 51 45 Cuts at: 2023 2199 2385 2619 2869 2943 3012 Size: 176 186 234 250 74 69 Fragments arranged by size: 521 250 250 234 231 220 186 176 153 127 114 105 74 69 63 60 51 45 36 23 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 130 406 667 802 874 1699 1909 2245 Size: 130 276 261 135 72 825 210 336 Cuts at: 2245 2364 3012 Size: 119 648 Fragments arranged by size: 825 648 336 276 261 210 135 130 119 72 EcoO109I rG'GnC_Cy Cuts at: 0 504 1998 2103 2811 3012 Size: 504 1494 105 708 201 Fragments arranged by size: 1494 708 504 201 105 FspI TGC'GCA Cuts at: 0 1198 3012 Size: 1198 1814 HaeII r_GCGC'y Cuts at: 0 200 2890 3012 Size: 200 2690 122 Fragments arranged by size: 2690 200 122 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 757 789 2600 2632 3012 Size: 757 32 1811 32 380 Fragments arranged by size: 1811 757 380 32 32 HincII GTy'rAC Cuts at: 0 330 1968 2049 2781 3012 Size: 330 1638 81 732 231 Fragments arranged by size: 1638 732 330 231 81 KasI G'GCGC_C Cuts at: 0 2886 3012 Size: 2886 126 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1349 2357 3012 Size: 1349 1008 655 Fragments arranged by size: 1349 1008 655 MscI TGG'CCA Cuts at: 0 2056 3012 Size: 2056 956 MslI CAynn'nnrTG Cuts at: 0 563 2437 3012 Size: 563 1874 575 Fragments arranged by size: 1874 575 563 MspA1I CmG'CkG Cuts at: 0 153 465 516 1215 1287 1502 1605 2648 Size: 153 312 51 699 72 215 103 1043 Cuts at: 2648 2718 2820 2948 3012 Size: 70 102 128 64 Fragments arranged by size: 1043 699 312 215 153 128 103 102 72 70 64 51 NarI GG'CG_CC Cuts at: 0 2887 3012 Size: 2887 125 NcoI C'CATG_G Cuts at: 0 2057 3012 Size: 2057 955 NsiI A_TGCA'T Cuts at: 0 1304 1734 3012 Size: 1304 430 1278 Fragments arranged by size: 1304 1278 430 NspI r_CATG'y Cuts at: 0 1306 2152 3012 Size: 1306 846 860 Fragments arranged by size: 1306 860 846 PasI CC'CwG_GG Cuts at: 0 2501 3012 Size: 2501 511 PflMI CCAn_nnn'nTGG Cuts at: 0 1134 2001 2290 2670 3012 Size: 1134 867 289 380 342 Fragments arranged by size: 1134 867 380 342 289 PfoI T'CCnGG_A Cuts at: 0 263 1514 1889 1994 2246 2621 2705 2846 Size: 263 1251 375 105 252 375 84 141 Cuts at: 2846 3012 Size: 166 Fragments arranged by size: 1251 375 375 263 252 166 141 105 84 PmlI CAC'GTG Cuts at: 0 2408 3012 Size: 2408 604 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1861 1893 3012 Size: 1861 32 1119 Fragments arranged by size: 1861 1119 32 PpuMI rG'GwC_Cy Cuts at: 0 1998 2103 3012 Size: 1998 105 909 Fragments arranged by size: 1998 909 105 PsiI TTA'TAA Cuts at: 0 1982 3012 Size: 1982 1030 PspXI vC'TCGA_Gb Cuts at: 0 236 3012 Size: 236 2776 PstI C_TGCA'G Cuts at: 0 320 407 470 629 668 701 995 1052 Size: 320 87 63 159 39 33 294 57 Cuts at: 1052 1073 1220 1336 1622 2087 3012 Size: 21 147 116 286 465 925 Fragments arranged by size: 925 465 320 294 286 159 147 116 87 63 57 39 33 21 PvuII CAG'CTG Cuts at: 0 153 465 516 1215 1502 1605 2718 2948 Size: 153 312 51 699 287 103 1113 230 Cuts at: 2948 3012 Size: 64 Fragments arranged by size: 1113 699 312 287 230 153 103 64 51 SacI G_AGCT'C Cuts at: 0 1580 3012 Size: 1580 1432 SbfI CC_TGCA'GG Cuts at: 0 407 668 3012 Size: 407 261 2344 Fragments arranged by size: 2344 407 261 ScaI AGT'ACT Cuts at: 0 964 2572 3012 Size: 964 1608 440 Fragments arranged by size: 1608 964 440 SexAI A'CCwGG_T Cuts at: 0 1181 3012 Size: 1181 1831 SfcI C'TryA_G Cuts at: 0 316 403 466 612 625 664 697 991 Size: 316 87 63 146 13 39 33 294 Cuts at: 991 1026 1048 1069 1216 1332 1618 2083 2815 Size: 35 22 21 147 116 286 465 732 Cuts at: 2815 3012 Size: 197 Fragments arranged by size: 732 465 316 294 286 197 147 146 116 87 63 39 35 33 22 21 13 SfoI GGC'GCC Cuts at: 0 2888 3012 Size: 2888 124 SmaI CCC'GGG Cuts at: 0 1529 1799 1832 3012 Size: 1529 270 33 1180 Fragments arranged by size: 1529 1180 270 33 SmlI C'TyrA_G Cuts at: 0 236 1240 2574 2803 3012 Size: 236 1004 1334 229 209 Fragments arranged by size: 1334 1004 236 229 209 SphI G_CATG'C Cuts at: 0 1306 2152 3012 Size: 1306 846 860 Fragments arranged by size: 1306 860 846 SrfI GCCC'GGGC Cuts at: 0 1832 3012 Size: 1832 1180 StuI AGG'CCT Cuts at: 0 2596 3012 Size: 2596 416 StyI C'CwwG_G Cuts at: 0 137 2057 2336 2544 3012 Size: 137 1920 279 208 468 Fragments arranged by size: 1920 468 279 208 137 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 388 1852 2643 2710 3012 Size: 388 1464 791 67 302 Fragments arranged by size: 1464 791 388 302 67 TatI w'GTAC_w Cuts at: 0 962 1451 2570 3012 Size: 962 489 1119 442 Fragments arranged by size: 1119 962 489 442 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 40 72 3012 Size: 40 32 2940 Fragments arranged by size: 2940 40 32 Tth111I GACn'n_nGTC Cuts at: 0 1183 2725 3012 Size: 1183 1542 287 Fragments arranged by size: 1542 1183 287 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 261 1395 3012 Size: 261 1134 1617 Fragments arranged by size: 1617 1134 261 XhoI C'TCGA_G Cuts at: 0 236 3012 Size: 236 2776 XmaI C'CCGG_G Cuts at: 0 1527 1797 1830 3012 Size: 1527 270 33 1182 Fragments arranged by size: 1527 1182 270 33 ZraI GAC'GTC Cuts at: 0 294 2712 3012 Size: 294 2418 300 Fragments arranged by size: 2418 300 294 Enzymes that do cut: AarI AatII AccI AcuI AhdI AleI AlfI AlwNI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NarI NcoI NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI PsiI PspXI PstI PvuII SacI SbfI ScaI SexAI SfcI SfoI SmaI SmlI SphI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AflIII AgeI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BclI BglII BmtI BsaWI BsiEI BspEI BspHI BsrFI BssHII BstBI BstZ17I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PshAI PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI SfiI SgfI SgrAI SnaBI SpeI SspI SwaI XbaI XmnI