Restriction map for
pFN21AE3340
(Linear) (Six-base) MAPSORT of: KIEE3340.seq Check: 5275 from: 1 to: 2952
KIEE3340.orf
With 182 enzymes: SgfI *
March 22, 2012 16:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 769 2952
Size: 769 2183
AatII G_ACGT'C
Cuts at: 0 1009 2952
Size: 1009 1943
AccI GT'mk_AC
Cuts at: 0 410 2952
Size: 410 2542
AclI AA'CG_TT
Cuts at: 0 61 2952
Size: 61 2891
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1978 2390 2952
Size: 863 1115 412 562
Fragments arranged by size:
1115 863 562 412
AgeI A'CCGG_T
Cuts at: 0 913 2952
Size: 913 2039
AleI CACnn'nnGTG
Cuts at: 0 2686 2952
Size: 2686 266
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 623 2952
Size: 623 2329
AlwNI CAG_nnn'CTG
Cuts at: 0 46 1575 2703 2952
Size: 46 1529 1128 249
Fragments arranged by size:
1529 1128 249 46
ApaI G_GGCC'C
Cuts at: 0 246 1194 2952
Size: 246 948 1758
Fragments arranged by size:
1758 948 246
ApoI r'AATT_y
Cuts at: 0 603 2952
Size: 603 2349
AvaI C'yCGr_G
Cuts at: 0 662 2952
Size: 662 2290
BamHI G'GATC_C
Cuts at: 0 310 1803 2952
Size: 310 1493 1149
Fragments arranged by size:
1493 1149 310
BanI G'GyrC_C
Cuts at: 0 741 1025 2952
Size: 741 284 1927
Fragments arranged by size:
1927 741 284
BanII G_rGCy'C
Cuts at: 0 197 246 1194 2952
Size: 197 49 948 1758
Fragments arranged by size:
1758 948 197 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 180 564 2852 2952
Size: 180 384 2288 100
Fragments arranged by size:
2288 384 180 100
BbvCI CC'TCA_GC
Cuts at: 0 1603 2952
Size: 1603 1349
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 171 205 743 777 1952 1986 2952
Size: 171 34 538 34 1175 34 966
Fragments arranged by size:
1175 966 538 171 34 34 34
BclI T'GATC_A
Cuts at: 0 2081 2952
Size: 2081 871
BfrBI ATG'CAT
Cuts at: 0 674 2952
Size: 674 2278
BglI GCCn_nnn'nGGC
Cuts at: 0 750 2952
Size: 750 2202
BglII A'GATC_T
Cuts at: 0 1775 2952
Size: 1775 1177
BlpI GC'TnA_GC
Cuts at: 0 2758 2952
Size: 2758 194
Bme1580I G_kGCm'C
Cuts at: 0 246 670 704 1142 1194 1524 2952
Size: 246 424 34 438 52 330 1428
Fragments arranged by size:
1428 438 424 330 246 52 34
BmgBI CAC'GTC
Cuts at: 0 699 1266 2952
Size: 699 567 1686
Fragments arranged by size:
1686 699 567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 369 453 665 1455 2952
Size: 369 84 212 790 1497
Fragments arranged by size:
1497 790 369 212 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 926 1039 1221 1720 2068 2290 2360 2803
Size: 926 113 182 499 348 222 70 443
Cuts at: 2803 2952
Size: 149
Fragments arranged by size:
926 499 443 348 222 182 149 113
70
Bpu10I CC'TnA_GC
Cuts at: 0 1603 2173 2952
Size: 1603 570 779
Fragments arranged by size:
1603 779 570
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2647 2952
Size: 2647 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 316 2460 2952
Size: 316 2144 492
Fragments arranged by size:
2144 492 316
BsaAI yAC'GTr
Cuts at: 0 1941 2952
Size: 1941 1011
BsaBI GATnn'nnATC
Cuts at: 0 1774 2952
Size: 1774 1178
BsaHI Gr'CG_yC
Cuts at: 0 889 1006 2952
Size: 889 117 1946
Fragments arranged by size:
1946 889 117
BsaWI w'CCGG_w
Cuts at: 0 913 1406 2591 2645 2952
Size: 913 493 1185 54 307
Fragments arranged by size:
1185 913 493 307 54
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 8 11 38 41 847 859 877 889
Size: 8 3 27 3 806 12 18 12
Cuts at: 889 2323 2353 2952
Size: 1434 30 599
Fragments arranged by size:
1434 806 599 30 27 18 12 12
8 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 1356 1431 2952
Size: 16 1340 75 1521
Fragments arranged by size:
1521 1340 75 16
BseYI C'CCAG_C
Cuts at: 0 93 589 1149 2696 2952
Size: 93 496 560 1547 256
Fragments arranged by size:
1547 560 496 256 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 885 2952
Size: 885 2067
BsiEI CG_ry'CG
Cuts at: 0 1643 2712 2952
Size: 1643 1069 240
Fragments arranged by size:
1643 1069 240
BsiHKAI G_wGCw'C
Cuts at: 0 646 2952
Size: 646 2306
BsmI GAATG_Cn'
Cuts at: 0 2903 2952
Size: 2903 49
BsmBI CGTCTCn'nnnn_
Cuts at: 0 896 997 1176 1272 2952
Size: 896 101 179 96 1680
Fragments arranged by size:
1680 896 179 101 96
Bsp1286I G_dGCh'C
Cuts at: 0 197 246 646 670 704 1142 1194 1524
Size: 197 49 400 24 34 438 52 330
Cuts at: 1524 2952
Size: 1428
Fragments arranged by size:
1428 438 400 330 197 52 49 34
24
BspEI T'CCGG_A
Cuts at: 0 2645 2952
Size: 2645 307
BspHI T'CATG_A
Cuts at: 0 2078 2952
Size: 2078 874
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 769 2952
Size: 769 2183
BsrBI CCG'CTC
Cuts at: 0 875 928 2714 2952
Size: 875 53 1786 238
Fragments arranged by size:
1786 875 238 53
BsrDI GCAATG_nn'
Cuts at: 0 189 359 416 987 2951 2952
Size: 189 170 57 571 1964 1
Fragments arranged by size:
1964 571 189 170 57 1
BsrFI r'CCGG_y
Cuts at: 0 233 913 2522 2600 2952
Size: 233 680 1609 78 352
Fragments arranged by size:
1609 680 352 233 78
BsrGI T'GTAC_A
Cuts at: 0 1721 1898 2952
Size: 1721 177 1054
Fragments arranged by size:
1721 1054 177
BstAPI GCAn_nnn'nTGC
Cuts at: 0 46 806 1085 1562 2952
Size: 46 760 279 477 1390
Fragments arranged by size:
1390 760 477 279 46
BstEII G'GTnAC_C
Cuts at: 0 2058 2826 2952
Size: 2058 768 126
Fragments arranged by size:
2058 768 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1017 1149 2343 2772 2952
Size: 1017 132 1194 429 180
Fragments arranged by size:
1194 1017 429 180 132
BstYI r'GATC_y
Cuts at: 0 310 1403 1775 1803 2322 2354 2891 2952
Size: 310 1093 372 28 519 32 537 61
Fragments arranged by size:
1093 537 519 372 310 61 32 28
Bsu36I CC'TnA_GG
Cuts at: 0 2125 2952
Size: 2125 827
BtgI C'CryG_G
Cuts at: 0 1445 2537 2744 2952
Size: 1445 1092 207 208
Fragments arranged by size:
1445 1092 208 207
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 64 232 768 2166 2370 2952
Size: 64 168 536 1398 204 582
Fragments arranged by size:
1398 582 536 204 168 64
BtsI GCAGTG_nn'
Cuts at: 0 47 617 855 2798 2952
Size: 47 570 238 1943 154
Fragments arranged by size:
1943 570 238 154 47
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1533 1568 2952
Size: 1533 35 1384
Fragments arranged by size:
1533 1384 35
EaeI y'GGCC_r
Cuts at: 0 104 1360 1448 1640 1670 1943 2952
Size: 104 1256 88 192 30 273 1009
Fragments arranged by size:
1256 1009 273 192 104 88 30
EagI C'GGCC_G
Cuts at: 0 1640 2952
Size: 1640 1312
EarI CTCTTCn'nnn_
Cuts at: 0 560 1859 2952
Size: 560 1299 1093
Fragments arranged by size:
1299 1093 560
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 926 1039 1221 1720 1978 2068 2290
Size: 863 63 113 182 499 258 90 222
Cuts at: 2290 2360 2390 2803 2952
Size: 70 30 413 149
Fragments arranged by size:
863 499 413 258 222 182 149 113
90 70 63 30
EcoO109I rG'GnC_Cy
Cuts at: 0 242 393 973 2229 2952
Size: 242 151 580 1256 723
Fragments arranged by size:
1256 723 580 242 151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 342 396 1266 1581 2952
Size: 342 54 870 315 1371
Fragments arranged by size:
1371 870 342 315 54
HaeII r_GCGC'y
Cuts at: 0 2765 2952
Size: 2765 187
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 93 125 631 663 1579 1611 2302 2334
Size: 93 32 506 32 916 32 691 32
Cuts at: 2334 2339 2371 2952
Size: 5 32 581
Fragments arranged by size:
916 691 581 506 93 32 32 32
32 32 5
HincII GTy'rAC
Cuts at: 0 1275 2256 2625 2952
Size: 1275 981 369 327
Fragments arranged by size:
1275 981 369 327
MfeI C'AATT_G
Cuts at: 0 207 2952
Size: 207 2745
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 890 2306 2510 2952
Size: 889 1 1416 204 442
Fragments arranged by size:
1416 889 442 204 1
MscI TGG'CCA
Cuts at: 0 1362 1450 1672 1945 2952
Size: 1362 88 222 273 1007
Fragments arranged by size:
1362 1007 273 222 88
MslI CAynn'nnrTG
Cuts at: 0 262 2686 2770 2833 2952
Size: 262 2424 84 63 119
Fragments arranged by size:
2424 262 119 84 63
MspA1I CmG'CkG
Cuts at: 0 1115 1149 1614 1639 2551 2823 2952
Size: 1115 34 465 25 912 272 129
Fragments arranged by size:
1115 912 465 272 129 34 25
NaeI GCC'GGC
Cuts at: 0 2602 2952
Size: 2602 350
NcoI C'CATG_G
Cuts at: 0 1445 2537 2952
Size: 1445 1092 415
Fragments arranged by size:
1445 1092 415
NgoMIV G'CCGG_C
Cuts at: 0 2600 2952
Size: 2600 352
NsiI A_TGCA'T
Cuts at: 0 676 2952
Size: 676 2276
NspI r_CATG'y
Cuts at: 0 674 800 956 2952
Size: 674 126 156 1996
Fragments arranged by size:
1996 674 156 126
PasI CC'CwG_GG
Cuts at: 0 1765 2952
Size: 1765 1187
PflMI CCAn_nnn'nTGG
Cuts at: 0 2163 2952
Size: 2163 789
PfoI T'CCnGG_A
Cuts at: 0 1391 1820 2306 2952
Size: 1391 429 486 646
Fragments arranged by size:
1391 646 486 429
PpuMI rG'GwC_Cy
Cuts at: 0 973 2229 2952
Size: 973 1256 723
Fragments arranged by size:
1256 973 723
PspOMI G'GGCC_C
Cuts at: 0 242 1190 2952
Size: 242 948 1762
Fragments arranged by size:
1762 948 242
PstI C_TGCA'G
Cuts at: 0 90 289 426 816 885 2952
Size: 90 199 137 390 69 2067
Fragments arranged by size:
2067 390 199 137 90 69
PvuII CAG'CTG
Cuts at: 0 1149 1614 2551 2823 2952
Size: 1149 465 937 272 129
Fragments arranged by size:
1149 937 465 272 129
RsrII CG'GwC_CG
Cuts at: 0 911 2952
Size: 911 2041
SbfI CC_TGCA'GG
Cuts at: 0 816 2952
Size: 816 2136
SexAI A'CCwGG_T
Cuts at: 0 173 2264 2952
Size: 173 2091 688
Fragments arranged by size:
2091 688 173
SfcI C'TryA_G
Cuts at: 0 86 285 422 812 881 1686 2952
Size: 86 199 137 390 69 805 1266
Fragments arranged by size:
1266 805 390 199 137 86 69
SmlI C'TyrA_G
Cuts at: 0 2662 2952
Size: 2662 290
SphI G_CATG'C
Cuts at: 0 800 956 2952
Size: 800 156 1996
Fragments arranged by size:
1996 800 156
StyI C'CwwG_G
Cuts at: 0 1033 1445 1664 1832 2342 2537 2870 2952
Size: 1033 412 219 168 510 195 333 82
Fragments arranged by size:
1033 510 412 333 219 195 168 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2550 2845 2952
Size: 2550 295 107
Fragments arranged by size:
2550 295 107
TatI w'GTAC_w
Cuts at: 0 1721 1898 2780 2952
Size: 1721 177 882 172
Fragments arranged by size:
1721 882 177 172
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 847 879 2307 2339 2952
Size: 847 32 1428 32 613
Fragments arranged by size:
1428 847 613 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 355 2952
Size: 355 2597
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 448 1225 2952
Size: 448 777 1727
Fragments arranged by size:
1727 777 448
ZraI GAC'GTC
Cuts at: 0 1007 2952
Size: 1007 1945
Enzymes that do cut:
AarI AatII AccI AclI AcuI AgeI AleI AlfI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI EaeI EagI EarI Eco57MI EcoO109I
FalI HaeII Hin4I HincII MfeI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NsiI NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII RsrII SbfI SexAI
SfcI SmlI SphI StyI TaqII TatI TstI Tth111I
XcmI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AhdI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BciVI BmrI BmtI
BsiWI BssHII BssSI BstBI BstZ17I ClaI DraI DraIII
DrdI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MluI NarI NdeI
NheI NotI NruI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI SacI SacII SalI
SanDI SapI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI XbaI XhoI
XmaI XmnI