Restriction map for
pFN21AE3331
(Linear) (Six-base) MAPSORT of: KIEE3331.seq Check: 10 from: 1 to: 2841
KIEE3331.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
Acc65I G'GTAC_C
Cuts at: 0 362 2841
Size: 362 2479
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 837 921 1089 1509 1593 1677 2097 2517
Size: 837 84 168 420 84 84 420 420
Cuts at: 2517 2841
Size: 324
Fragments arranged by size:
837 420 420 420 324 168 84 84
84
AhdI GACnn_n'nnGTC
Cuts at: 0 119 2841
Size: 119 2722
AleI CACnn'nnGTG
Cuts at: 0 306 1064 2172 2841
Size: 306 758 1108 669
Fragments arranged by size:
1108 758 669 306
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 324 908 2841
Size: 324 584 1933
Fragments arranged by size:
1933 584 324
ApaLI G'TGCA_C
Cuts at: 0 1981 2723 2763 2841
Size: 1981 742 40 78
Fragments arranged by size:
1981 742 78 40
ApoI r'AATT_y
Cuts at: 0 971 1055 1223 2231 2625 2841
Size: 971 84 168 1008 394 216
Fragments arranged by size:
1008 971 394 216 168 84
AvrII C'CTAG_G
Cuts at: 0 357 2841
Size: 357 2484
BanI G'GyrC_C
Cuts at: 0 362 2841
Size: 362 2479
BbsI GAAGACnn'nnnn_
Cuts at: 0 556 1095 2841
Size: 556 539 1746
Fragments arranged by size:
1746 556 539
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 145 179 2841
Size: 145 34 2662
Fragments arranged by size:
2662 145 34
BfrBI ATG'CAT
Cuts at: 0 2811 2841
Size: 2811 30
Bme1580I G_kGCm'C
Cuts at: 0 1985 2727 2767 2841
Size: 1985 742 40 74
Fragments arranged by size:
1985 742 74 40
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1594 2841
Size: 1594 1247
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2009 2841
Size: 2009 832
BsaBI GATnn'nnATC
Cuts at: 0 491 2841
Size: 491 2350
BsaWI w'CCGG_w
Cuts at: 0 14 2841
Size: 14 2827
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 2841
Size: 292 2549
BseYI C'CCAG_C
Cuts at: 0 297 513 2042 2841
Size: 297 216 1529 799
Fragments arranged by size:
1529 799 297 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 2196 2364 2841
Size: 445 1751 168 477
Fragments arranged by size:
1751 477 445 168
BsiHKAI G_wGCw'C
Cuts at: 0 1985 2727 2767 2841
Size: 1985 742 40 74
Fragments arranged by size:
1985 742 74 40
BsmI GAATG_Cn'
Cuts at: 0 544 1003 1012 1255 1591 1675 1759 2188
Size: 544 459 9 243 336 84 84 429
Cuts at: 2188 2263 2573 2599 2841
Size: 75 310 26 242
Fragments arranged by size:
544 459 429 336 310 243 242 84
84 75 26 9
Bsp1286I G_dGCh'C
Cuts at: 0 1985 2727 2767 2841
Size: 1985 742 40 74
Fragments arranged by size:
1985 742 74 40
BspEI T'CCGG_A
Cuts at: 0 14 2841
Size: 14 2827
BsrDI GCAATG_nn'
Cuts at: 0 1260 1512 2688 2841
Size: 1260 252 1176 153
Fragments arranged by size:
1260 1176 252 153
BsrGI T'GTAC_A
Cuts at: 0 314 960 1380 1464 1884 1968 2052 2472
Size: 314 646 420 84 420 84 84 420
Cuts at: 2472 2556 2640 2841
Size: 84 84 201
Fragments arranged by size:
646 420 420 420 314 201 84 84
84 84 84
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1007 2183 2841
Size: 1007 1176 658
Fragments arranged by size:
1176 1007 658
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1681 2841
Size: 1681 1160
BtsI GCAGTG_nn'
Cuts at: 0 1008 2100 2268 2352 2604 2841
Size: 1008 1092 168 84 252 237
Fragments arranged by size:
1092 1008 252 237 168 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1494 1529 1578 1613 2670 2705 2841
Size: 1494 35 49 35 1057 35 136
Fragments arranged by size:
1494 1057 136 49 35 35 35
DraI TTT'AAA
Cuts at: 0 1196 1617 1784 2841
Size: 1196 421 167 1057
Fragments arranged by size:
1196 1057 421 167
EaeI y'GGCC_r
Cuts at: 0 2 2841
Size: 2 2839
EarI CTCTTCn'nnn_
Cuts at: 0 154 404 893 2841
Size: 154 250 489 1948
Fragments arranged by size:
1948 489 250 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 837 921 1089 1509 1593 1677 2009 2097
Size: 837 84 168 420 84 84 332 88
Cuts at: 2097 2517 2841
Size: 420 324
Fragments arranged by size:
837 420 420 332 324 168 88 84
84 84
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2283 2619 2841
Size: 2283 336 222
Fragments arranged by size:
2283 336 222
EcoO109I rG'GnC_Cy
Cuts at: 0 33 235 2841
Size: 33 202 2606
Fragments arranged by size:
2606 202 33
EcoRI G'AATT_C
Cuts at: 0 971 1055 1223 2231 2625 2841
Size: 971 84 168 1008 394 216
Fragments arranged by size:
1008 971 394 216 168 84
EcoRV GAT'ATC
Cuts at: 0 384 2841
Size: 384 2457
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1138 2841
Size: 1138 1703
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 1872 1904 2841
Size: 105 32 1735 32 937
Fragments arranged by size:
1735 937 105 32 32
HincII GTy'rAC
Cuts at: 0 2499 2841
Size: 2499 342
HindIII A'AGCT_T
Cuts at: 0 531 1184 2360 2841
Size: 531 653 1176 481
Fragments arranged by size:
1176 653 531 481
KpnI G_GTAC'C
Cuts at: 0 366 2841
Size: 366 2475
MfeI C'AATT_G
Cuts at: 0 1593 2841
Size: 1593 1248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 311 377 1678 2770 2841
Size: 311 66 1301 1092 71
Fragments arranged by size:
1301 1092 311 71 66
MscI TGG'CCA
Cuts at: 0 4 2841
Size: 4 2837
MslI CAynn'nnrTG
Cuts at: 0 253 306 605 1064 1164 1248 1486 1679
Size: 253 53 299 459 100 84 238 193
Cuts at: 1679 1836 2088 2172 2408 2424 2592 2828 2841
Size: 157 252 84 236 16 168 236 13
Fragments arranged by size:
459 299 253 252 238 236 236 193
168 157 100 84 84 53 16 13
MspA1I CmG'CkG
Cuts at: 0 288 836 2841
Size: 288 548 2005
Fragments arranged by size:
2005 548 288
NdeI CA'TA_TG
Cuts at: 0 1161 1245 2085 2141 2421 2589 2841
Size: 1161 84 840 56 280 168 252
Fragments arranged by size:
1161 840 280 252 168 84 56
NsiI A_TGCA'T
Cuts at: 0 2813 2841
Size: 2813 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 230 2841
Size: 230 2611
PpuMI rG'GwC_Cy
Cuts at: 0 33 235 2841
Size: 33 202 2606
Fragments arranged by size:
2606 202 33
PshAI GACnn'nnGTC
Cuts at: 0 240 2841
Size: 240 2601
PsiI TTA'TAA
Cuts at: 0 2507 2841
Size: 2507 334
PvuII CAG'CTG
Cuts at: 0 288 836 2841
Size: 288 548 2005
Fragments arranged by size:
2005 548 288
SanDI GG'GwC_CC
Cuts at: 0 33 2841
Size: 33 2808
SapI GCTCTTCn'nnn_
Cuts at: 0 404 2841
Size: 404 2437
SexAI A'CCwGG_T
Cuts at: 0 347 2841
Size: 347 2494
SfcI C'TryA_G
Cuts at: 0 339 1917 2841
Size: 339 1578 924
Fragments arranged by size:
1578 924 339
SpeI A'CTAG_T
Cuts at: 0 646 2841
Size: 646 2195
StyI C'CwwG_G
Cuts at: 0 84 108 357 2841
Size: 84 24 249 2484
Fragments arranged by size:
2484 249 84 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 159 1406 2330 2666 2841
Size: 159 1247 924 336 175
Fragments arranged by size:
1247 924 336 175 159
TatI w'GTAC_w
Cuts at: 0 314 571 960 1380 1464 1884 1968 2052
Size: 314 257 389 420 84 420 84 84
Cuts at: 2052 2472 2556 2640 2841
Size: 420 84 84 201
Fragments arranged by size:
420 420 420 389 314 257 201 84
84 84 84 84
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 115 461 2841
Size: 115 346 2380
Fragments arranged by size:
2380 346 115
XmnI GAAnn'nnTTC
Cuts at: 0 57 850 934 1018 1102 1223 1606 1690
Size: 57 793 84 84 84 121 383 84
Cuts at: 1690 2110 2403 2446 2530 2655 2698 2841
Size: 420 293 43 84 125 43 143
Fragments arranged by size:
793 420 383 293 143 125 121 84
84 84 84 84 57 43 43
Enzymes that do cut:
Acc65I AcuI AhdI AleI AlfI ApaLI ApoI AvrII
BanI BbsI BcgI BfrBI Bme1580I BplI BpmI BsaBI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BsrDI BsrGI BstAPI BstXI BtsI CspCI DraI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NdeI NsiI PflMI PpuMI PshAI PsiI PvuII SanDI
SapI SexAI SfcI SpeI StyI TaqII TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AloI AlwNI ApaI AscI AseI AsiSI AvaI BaeI
BamHI BanII BbeI BbvCI BciVI BclI BglI BglII
BlpI BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI DraIII DrdI EagI EciI EcoICRI
FseI FspI FspAI HaeII HpaI KasI MluI NaeI
NarI NcoI NgoMIV NheI NotI NruI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI ZraI