Restriction map for
pFN21AE3313
(Linear) (Six-base) MAPSORT of: KIEE3313.seq Check: 9808 from: 1 to: 2541
KIEE3313.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AccI GT'mk_AC
Cuts at: 0 821 2541
Size: 821 1720
AclI AA'CG_TT
Cuts at: 0 686 2541
Size: 686 1855
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 977 1073 2541
Size: 977 96 1468
Fragments arranged by size:
1468 977 96
AflII C'TTAA_G
Cuts at: 0 567 2541
Size: 567 1974
AflIII A'CryG_T
Cuts at: 0 1397 1529 2541
Size: 1397 132 1012
Fragments arranged by size:
1397 1012 132
AlwNI CAG_nnn'CTG
Cuts at: 0 955 1681 2320 2541
Size: 955 726 639 221
Fragments arranged by size:
955 726 639 221
ApoI r'AATT_y
Cuts at: 0 322 469 826 850 1774 1912 1943 2338
Size: 322 147 357 24 924 138 31 395
Cuts at: 2338 2417 2541
Size: 79 124
Fragments arranged by size:
924 395 357 322 147 138 124 79
31 24
AseI AT'TA_AT
Cuts at: 0 331 514 1592 2541
Size: 331 183 1078 949
Fragments arranged by size:
1078 949 331 183
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 335 368 2541
Size: 335 33 2173
Fragments arranged by size:
2173 335 33
BamHI G'GATC_C
Cuts at: 0 1824 2541
Size: 1824 717
BbsI GAAGACnn'nnnn_
Cuts at: 0 250 2541
Size: 250 2291
BclI T'GATC_A
Cuts at: 0 1392 2541
Size: 1392 1149
BglI GCCn_nnn'nGGC
Cuts at: 0 1678 2541
Size: 1678 863
BglII A'GATC_T
Cuts at: 0 1884 2541
Size: 1884 657
BmrI ACTGGGnnnn_n'
Cuts at: 0 2442 2541
Size: 2442 99
BmtI G_CTAG'C
Cuts at: 0 914 2541
Size: 914 1627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 2541
Size: 96 2445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1130 2541
Size: 1130 1411
BsaI GGTCTCn'nnnn_
Cuts at: 0 272 2541
Size: 272 2269
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 2541
Size: 139 30 2372
Fragments arranged by size:
2372 139 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 1274 2541
Size: 160 1114 1267
Fragments arranged by size:
1267 1114 160
BsmI GAATG_Cn'
Cuts at: 0 2020 2541
Size: 2020 521
BspHI T'CATG_A
Cuts at: 0 2043 2541
Size: 2043 498
BsrDI GCAATG_nn'
Cuts at: 0 944 1046 2541
Size: 944 102 1495
Fragments arranged by size:
1495 944 102
BsrGI T'GTAC_A
Cuts at: 0 136 2541
Size: 136 2405
BstBI TT'CG_AA
Cuts at: 0 714 2541
Size: 714 1827
BstXI CCAn_nnnn'nTGG
Cuts at: 0 74 2541
Size: 74 2467
BstYI r'GATC_y
Cuts at: 0 1491 1700 1824 1884 2541
Size: 1491 209 124 60 657
Fragments arranged by size:
1491 657 209 124 60
BstZ17I GTA'TAC
Cuts at: 0 822 2541
Size: 822 1719
BtgI C'CryG_G
Cuts at: 0 2035 2541
Size: 2035 506
BtsI GCAGTG_nn'
Cuts at: 0 1034 2541
Size: 1034 1507
DraI TTT'AAA
Cuts at: 0 2112 2541
Size: 2112 429
DraIII CAC_nnn'GTG
Cuts at: 0 1336 2541
Size: 1336 1205
EaeI y'GGCC_r
Cuts at: 0 1859 2541
Size: 1859 682
EarI CTCTTCn'nnn_
Cuts at: 0 305 2228 2541
Size: 305 1923 313
Fragments arranged by size:
1923 313 305
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 977 1073 2541
Size: 96 881 96 1468
Fragments arranged by size:
1468 881 96 96
EcoO109I rG'GnC_Cy
Cuts at: 0 940 952 1002 2541
Size: 940 12 50 1539
Fragments arranged by size:
1539 940 50 12
EcoRV GAT'ATC
Cuts at: 0 727 1307 2541
Size: 727 580 1234
Fragments arranged by size:
1234 727 580
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 202 1103 2348 2541
Size: 202 901 1245 193
Fragments arranged by size:
1245 901 202 193
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1603 1635 2541
Size: 1603 32 906
Fragments arranged by size:
1603 906 32
HincII GTy'rAC
Cuts at: 0 594 684 2059 2477 2541
Size: 594 90 1375 418 64
Fragments arranged by size:
1375 594 418 90 64
HindIII A'AGCT_T
Cuts at: 0 229 2541
Size: 229 2312
MfeI C'AATT_G
Cuts at: 0 2142 2541
Size: 2142 399
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2277 2541
Size: 2277 264
MscI TGG'CCA
Cuts at: 0 1861 2541
Size: 1861 680
MslI CAynn'nnrTG
Cuts at: 0 1109 2541
Size: 1109 1432
NcoI C'CATG_G
Cuts at: 0 2035 2541
Size: 2035 506
NheI G'CTAG_C
Cuts at: 0 910 2541
Size: 910 1631
NspI r_CATG'y
Cuts at: 0 1401 2541
Size: 1401 1140
PasI CC'CwG_GG
Cuts at: 0 2273 2541
Size: 2273 268
PciI A'CATG_T
Cuts at: 0 1397 2541
Size: 1397 1144
PfoI T'CCnGG_A
Cuts at: 0 73 2541
Size: 73 2468
PpuMI rG'GwC_Cy
Cuts at: 0 940 952 1002 2541
Size: 940 12 50 1539
Fragments arranged by size:
1539 940 50 12
PsiI TTA'TAA
Cuts at: 0 859 2541
Size: 859 1682
SanDI GG'GwC_CC
Cuts at: 0 940 2541
Size: 940 1601
ScaI AGT'ACT
Cuts at: 0 1635 1650 2541
Size: 1635 15 891
Fragments arranged by size:
1635 891 15
SmlI C'TyrA_G
Cuts at: 0 567 1145 2541
Size: 567 578 1396
Fragments arranged by size:
1396 578 567
SpeI A'CTAG_T
Cuts at: 0 414 2541
Size: 414 2127
SspI AAT'ATT
Cuts at: 0 1873 2541
Size: 1873 668
StyI C'CwwG_G
Cuts at: 0 2035 2541
Size: 2035 506
TatI w'GTAC_w
Cuts at: 0 136 1633 1648 2541
Size: 136 1497 15 893
Fragments arranged by size:
1497 893 136 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 1775 1807 2541
Size: 2 32 1741 32 734
Fragments arranged by size:
1741 734 32 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2270 2541
Size: 2270 271
XmnI GAAnn'nnTTC
Cuts at: 0 887 1874 2541
Size: 887 987 667
Fragments arranged by size:
987 887 667
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AlwNI ApoI AseI
BaeI BamHI BbsI BclI BglI BglII BmrI BmtI
BpmI BpuEI BsaI BsaXI BseRI BsmI BspHI BsrDI
BsrGI BstBI BstXI BstYI BstZ17I BtgI BtsI DraI
DraIII EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I
HincII HindIII MfeI MmeI MscI MslI NcoI NheI
NspI PasI PciI PfoI PpuMI PsiI SanDI ScaI
SmlI SpeI SspI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BanI
BanII BbeI BbvCI BcgI BciVI BfrBI BlpI Bme1580I
BmgBI BplI Bpu10I BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstEII Bsu36I BtgZI
ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MspA1I NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI