Restriction map for
pFN21AE3310
(Linear) (Six-base) MAPSORT of: KIEE3310.seq Check: 2807 from: 1 to: 2457
KIEE3310.orf
With 182 enzymes: SgfI *
July 3, 2012 17:37 ..
AatII G_ACGT'C
Cuts at: 0 1676 2457
Size: 1676 781
AccI GT'mk_AC
Cuts at: 0 2358 2457
Size: 2358 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1933 1975 2457
Size: 1933 42 482
Fragments arranged by size:
1933 482 42
AflII C'TTAA_G
Cuts at: 0 1792 2457
Size: 1792 665
AhdI GACnn_n'nnGTC
Cuts at: 0 1389 1575 2457
Size: 1389 186 882
Fragments arranged by size:
1389 882 186
AlwNI CAG_nnn'CTG
Cuts at: 0 124 205 949 1537 2457
Size: 124 81 744 588 920
Fragments arranged by size:
920 744 588 124 81
ApoI r'AATT_y
Cuts at: 0 1459 2457
Size: 1459 998
AseI AT'TA_AT
Cuts at: 0 2041 2457
Size: 2041 416
AvaI C'yCGr_G
Cuts at: 0 890 2457
Size: 890 1567
BamHI G'GATC_C
Cuts at: 0 837 2457
Size: 837 1620
BanI G'GyrC_C
Cuts at: 0 575 692 770 1648 2457
Size: 575 117 78 878 809
Fragments arranged by size:
878 809 575 117 78
BanII G_rGCy'C
Cuts at: 0 221 491 2457
Size: 221 270 1966
Fragments arranged by size:
1966 270 221
BbsI GAAGACnn'nnnn_
Cuts at: 0 1306 1724 2457
Size: 1306 418 733
Fragments arranged by size:
1306 733 418
BbvCI CC'TCA_GC
Cuts at: 0 1712 2457
Size: 1712 745
BciVI GTATCCnnnnn_n'
Cuts at: 0 1014 2457
Size: 1014 1443
BglII A'GATC_T
Cuts at: 0 1482 2121 2407 2457
Size: 1482 639 286 50
Fragments arranged by size:
1482 639 286 50
Bme1580I G_kGCm'C
Cuts at: 0 2250 2457
Size: 2250 207
BmrI ACTGGGnnnn_n'
Cuts at: 0 46 2457
Size: 46 2411
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 2457
Size: 126 2331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 2249 2457
Size: 475 1774 208
Fragments arranged by size:
1774 475 208
Bpu10I CC'TnA_GC
Cuts at: 0 1712 2457
Size: 1712 745
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1876 2457
Size: 1876 581
BsaI GGTCTCn'nnnn_
Cuts at: 0 364 1942 2177 2457
Size: 364 1578 235 280
Fragments arranged by size:
1578 364 280 235
BsaBI GATnn'nnATC
Cuts at: 0 338 2457
Size: 338 2119
BsaHI Gr'CG_yC
Cuts at: 0 1673 2457
Size: 1673 784
BsaWI w'CCGG_w
Cuts at: 0 112 2457
Size: 112 2345
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1371 1401 1999 2029 2457
Size: 1371 30 598 30 428
Fragments arranged by size:
1371 598 428 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 499 616 655 733 889 1111 1168
Size: 268 231 117 39 78 156 222 57
Cuts at: 1168 1171 1918 2224 2457
Size: 3 747 306 233
Fragments arranged by size:
747 306 268 233 231 222 156 117
78 57 39 3
BseYI C'CCAG_C
Cuts at: 0 1330 2457
Size: 1330 1127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 1871 2396 2457
Size: 320 1551 525 61
Fragments arranged by size:
1551 525 320 61
BsiHKAI G_wGCw'C
Cuts at: 0 491 2457
Size: 491 1966
Bsp1286I G_dGCh'C
Cuts at: 0 221 491 2250 2457
Size: 221 270 1759 207
Fragments arranged by size:
1759 270 221 207
BspEI T'CCGG_A
Cuts at: 0 112 2457
Size: 112 2345
BsrDI GCAATG_nn'
Cuts at: 0 1404 2457
Size: 1404 1053
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1929 2457
Size: 1929 528
BstYI r'GATC_y
Cuts at: 0 837 1368 1482 1895 1916 2121 2232 2407
Size: 837 531 114 413 21 205 111 175
Cuts at: 2407 2457
Size: 50
Fragments arranged by size:
837 531 413 205 175 114 111 50
21
Bsu36I CC'TnA_GG
Cuts at: 0 656 2457
Size: 656 1801
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1655 2457
Size: 1655 802
BtsI GCAGTG_nn'
Cuts at: 0 1985 2426 2457
Size: 1985 441 31
Fragments arranged by size:
1985 441 31
DraIII CAC_nnn'GTG
Cuts at: 0 2421 2457
Size: 2421 36
DrdI GACnn_nn'nnGTC
Cuts at: 0 2287 2457
Size: 2287 170
EarI CTCTTCn'nnn_
Cuts at: 0 362 490 791 1165 1202 1222 1355 1442
Size: 362 128 301 374 37 20 133 87
Cuts at: 1442 2175 2457
Size: 733 282
Fragments arranged by size:
733 374 362 301 282 133 128 87
37 20
EcoICRI GAG'CTC
Cuts at: 0 489 2457
Size: 489 1968
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1933 1975 2249 2457
Size: 475 1458 42 274 208
Fragments arranged by size:
1458 475 274 208 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2191 2457
Size: 2191 266
EcoO109I rG'GnC_Cy
Cuts at: 0 759 916 928 1803 2259 2457
Size: 759 157 12 875 456 198
Fragments arranged by size:
875 759 456 198 157 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1429 2457
Size: 1429 1028
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 265 271 297 303 943 975
Size: 61 32 172 6 26 6 640 32
Cuts at: 975 1201 1233 1258 1290 1375 1407 1561 1593
Size: 226 32 25 32 85 32 154 32
Cuts at: 1593 1999 2031 2457
Size: 406 32 426
Fragments arranged by size:
640 426 406 226 172 154 85 61
32 32 32 32 32 32 32 26
25 6 6
HincII GTy'rAC
Cuts at: 0 2359 2457
Size: 2359 98
MslI CAynn'nnrTG
Cuts at: 0 428 2457
Size: 428 2029
MspA1I CmG'CkG
Cuts at: 0 20 2457
Size: 20 2437
NdeI CA'TA_TG
Cuts at: 0 1061 1565 2457
Size: 1061 504 892
Fragments arranged by size:
1061 892 504
NspI r_CATG'y
Cuts at: 0 427 2457
Size: 427 2030
PfoI T'CCnGG_A
Cuts at: 0 1898 2235 2457
Size: 1898 337 222
Fragments arranged by size:
1898 337 222
PpuMI rG'GwC_Cy
Cuts at: 0 759 1803 2259 2457
Size: 759 1044 456 198
Fragments arranged by size:
1044 759 456 198
SacI G_AGCT'C
Cuts at: 0 491 2457
Size: 491 1966
SalI G'TCGA_C
Cuts at: 0 2357 2457
Size: 2357 100
SanDI GG'GwC_CC
Cuts at: 0 2259 2457
Size: 2259 198
SapI GCTCTTCn'nnn_
Cuts at: 0 791 2457
Size: 791 1666
SexAI A'CCwGG_T
Cuts at: 0 384 2457
Size: 384 2073
SfcI C'TryA_G
Cuts at: 0 1778 2457
Size: 1778 679
SmlI C'TyrA_G
Cuts at: 0 1792 1891 2457
Size: 1792 99 566
Fragments arranged by size:
1792 566 99
SpeI A'CTAG_T
Cuts at: 0 157 2457
Size: 157 2300
SphI G_CATG'C
Cuts at: 0 427 2457
Size: 427 2030
SspI AAT'ATT
Cuts at: 0 13 2457
Size: 13 2444
StuI AGG'CCT
Cuts at: 0 2061 2457
Size: 2061 396
StyI C'CwwG_G
Cuts at: 0 571 688 844 2003 2457
Size: 571 117 156 1159 454
Fragments arranged by size:
1159 571 454 156 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2190 2457
Size: 2190 267
TatI w'GTAC_w
Cuts at: 0 54 2457
Size: 54 2403
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 179 211 1371 1403 1880 1912 1964 1996
Size: 179 32 1160 32 477 32 52 32
Cuts at: 1996 2457
Size: 461
Fragments arranged by size:
1160 477 461 179 52 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1672 2457
Size: 1672 785
XbaI T'CTAG_A
Cuts at: 0 2124 2457
Size: 2124 333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 34 2184 2304 2457
Size: 34 2150 120 153
Fragments arranged by size:
2150 153 120 34
XmnI GAAnn'nnTTC
Cuts at: 0 800 2457
Size: 800 1657
ZraI GAC'GTC
Cuts at: 0 1674 2457
Size: 1674 783
Enzymes that do cut:
AatII AccI AcuI AflII AhdI AlwNI ApoI AseI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BsrDI BstAPI BstYI Bsu36I BtgZI BtsI DraIII
DrdI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I
HincII MslI MspA1I NdeI NspI PfoI PpuMI SacI
SalI SanDI SapI SexAI SfcI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvrII BaeI BbeI
BcgI BclI BfrBI BglI BlpI BmgBI BmtI BsaAI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtgI
ClaI CspCI DraI EaeI EagI EciI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacII SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI XhoI
XmaI