(Linear) (Six-base) MAPSORT of: KIEE3307.seq Check: 1616 from: 1 to: 2394 KIEE3307.orf With 182 enzymes: SgfI * July 3, 2012 17:37 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1146 2334 2394 Size: 1146 1188 60 Fragments arranged by size: 1188 1146 60 AfeI AGC'GCT Cuts at: 0 298 2394 Size: 298 2096 AgeI A'CCGG_T Cuts at: 0 2134 2394 Size: 2134 260 AlwNI CAG_nnn'CTG Cuts at: 0 1113 2394 Size: 1113 1281 ApaLI G'TGCA_C Cuts at: 0 1684 2394 Size: 1684 710 ApoI r'AATT_y Cuts at: 0 1810 2394 Size: 1810 584 AvaI C'yCGr_G Cuts at: 0 15 529 603 2394 Size: 15 514 74 1791 Fragments arranged by size: 1791 514 74 15 BamHI G'GATC_C Cuts at: 0 1240 1567 2394 Size: 1240 327 827 Fragments arranged by size: 1240 827 327 BanI G'GyrC_C Cuts at: 0 568 2394 Size: 568 1826 BanII G_rGCy'C Cuts at: 0 315 360 2394 Size: 315 45 2034 Fragments arranged by size: 2034 315 45 BbsI GAAGACnn'nnnn_ Cuts at: 0 1850 2394 Size: 1850 544 BbvCI CC'TCA_GC Cuts at: 0 436 448 594 2394 Size: 436 12 146 1800 Fragments arranged by size: 1800 436 146 12 BclI T'GATC_A Cuts at: 0 1509 2394 Size: 1509 885 BlpI GC'TnA_GC Cuts at: 0 1330 2394 Size: 1330 1064 Bme1580I G_kGCm'C Cuts at: 0 263 1688 2394 Size: 263 1425 706 Fragments arranged by size: 1425 706 263 BmrI ACTGGGnnnn_n' Cuts at: 0 1962 2394 Size: 1962 432 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 664 2394 Size: 664 1730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1012 2394 Size: 1012 1382 Bpu10I CC'TnA_GC Cuts at: 0 436 448 594 2394 Size: 436 12 146 1800 Fragments arranged by size: 1800 436 146 12 BsaBI GATnn'nnATC Cuts at: 0 1609 2394 Size: 1609 785 BsaHI Gr'CG_yC Cuts at: 0 152 342 2394 Size: 152 190 2052 Fragments arranged by size: 2052 190 152 BsaWI w'CCGG_w Cuts at: 0 116 926 1636 2134 2394 Size: 116 810 710 498 260 Fragments arranged by size: 810 710 498 260 116 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 692 888 1039 1302 1305 1708 2394 Size: 692 196 151 263 3 403 686 Fragments arranged by size: 692 686 403 263 196 151 3 BseYI C'CCAG_C Cuts at: 0 274 415 483 1083 2029 2195 2394 Size: 274 141 68 600 946 166 199 Fragments arranged by size: 946 600 274 199 166 141 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 396 2394 Size: 396 1998 BsiEI CG_ry'CG Cuts at: 0 79 255 432 566 2394 Size: 79 176 177 134 1828 Fragments arranged by size: 1828 177 176 134 79 BsiHKAI G_wGCw'C Cuts at: 0 315 360 1296 1688 1940 1967 2228 2394 Size: 315 45 936 392 252 27 261 166 Fragments arranged by size: 936 392 315 261 252 166 45 27 BsmI GAATG_Cn' Cuts at: 0 1134 1402 1880 2394 Size: 1134 268 478 514 Fragments arranged by size: 1134 514 478 268 Bsp1286I G_dGCh'C Cuts at: 0 263 315 360 1296 1688 1940 1967 2228 Size: 263 52 45 936 392 252 27 261 Cuts at: 2228 2394 Size: 166 Fragments arranged by size: 936 392 263 261 252 166 52 45 27 BspEI T'CCGG_A Cuts at: 0 926 2394 Size: 926 1468 BspHI T'CATG_A Cuts at: 0 1274 2171 2394 Size: 1274 897 223 Fragments arranged by size: 1274 897 223 BsrBI CCG'CTC Cuts at: 0 396 608 2394 Size: 396 212 1786 Fragments arranged by size: 1786 396 212 BsrDI GCAATG_nn' Cuts at: 0 1428 1535 2394 Size: 1428 107 859 Fragments arranged by size: 1428 859 107 BsrFI r'CCGG_y Cuts at: 0 29 269 1265 1766 2134 2394 Size: 29 240 996 501 368 260 Fragments arranged by size: 996 501 368 260 240 29 BstAPI GCAn_nnn'nTGC Cuts at: 0 1375 2394 Size: 1375 1019 BstXI CCAn_nnnn'nTGG Cuts at: 0 299 2394 Size: 299 2095 BstYI r'GATC_y Cuts at: 0 1240 1567 1699 2394 Size: 1240 327 132 695 Fragments arranged by size: 1240 695 327 132 Bsu36I CC'TnA_GG Cuts at: 0 721 761 2394 Size: 721 40 1633 Fragments arranged by size: 1633 721 40 BtgI C'CryG_G Cuts at: 0 2114 2394 Size: 2114 280 BtsI GCAGTG_nn' Cuts at: 0 103 1220 1916 1924 2394 Size: 103 1117 696 8 470 Fragments arranged by size: 1117 696 470 103 8 DraI TTT'AAA Cuts at: 0 1068 1782 2394 Size: 1068 714 612 Fragments arranged by size: 1068 714 612 DraIII CAC_nnn'GTG Cuts at: 0 388 2394 Size: 388 2006 DrdI GACnn_nn'nnGTC Cuts at: 0 845 1194 2394 Size: 845 349 1200 Fragments arranged by size: 1200 845 349 EaeI y'GGCC_r Cuts at: 0 76 252 429 459 2394 Size: 76 176 177 30 1935 Fragments arranged by size: 1935 177 176 76 30 EagI C'GGCC_G Cuts at: 0 76 252 429 2394 Size: 76 176 177 1965 Fragments arranged by size: 1965 177 176 76 EarI CTCTTCn'nnn_ Cuts at: 0 1009 1801 2008 2394 Size: 1009 792 207 386 Fragments arranged by size: 1009 792 386 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 252 395 564 2394 Size: 252 143 169 1830 Fragments arranged by size: 1830 252 169 143 EcoICRI GAG'CTC Cuts at: 0 313 358 2394 Size: 313 45 2036 Fragments arranged by size: 2036 313 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1012 1146 2334 2394 Size: 1012 134 1188 60 Fragments arranged by size: 1188 1012 134 60 EcoO109I rG'GnC_Cy Cuts at: 0 19 533 1110 1411 2124 2394 Size: 19 514 577 301 713 270 Fragments arranged by size: 713 577 514 301 270 19 HaeII r_GCGC'y Cuts at: 0 300 2394 Size: 300 2094 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 628 660 921 953 1008 1040 2394 Size: 628 32 261 32 55 32 1354 Fragments arranged by size: 1354 628 261 55 32 32 32 MfeI C'AATT_G Cuts at: 0 1502 2394 Size: 1502 892 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 203 423 2394 Size: 203 220 1971 Fragments arranged by size: 1971 220 203 MslI CAynn'nnrTG Cuts at: 0 694 2394 Size: 694 1700 MspA1I CmG'CkG Cuts at: 0 72 140 2033 2195 2394 Size: 72 68 1893 162 199 Fragments arranged by size: 1893 199 162 72 68 NaeI GCC'GGC Cuts at: 0 31 2394 Size: 31 2363 NcoI C'CATG_G Cuts at: 0 2114 2394 Size: 2114 280 NdeI CA'TA_TG Cuts at: 0 45 2394 Size: 45 2349 NgoMIV G'CCGG_C Cuts at: 0 29 2394 Size: 29 2365 PasI CC'CwG_GG Cuts at: 0 467 2394 Size: 467 1927 PflMI CCAn_nnn'nTGG Cuts at: 0 1083 2394 Size: 1083 1311 PfoI T'CCnGG_A Cuts at: 0 2321 2394 Size: 2321 73 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2338 2370 2394 Size: 2338 32 24 Fragments arranged by size: 2338 32 24 PpuMI rG'GwC_Cy Cuts at: 0 19 533 1110 1411 2124 2394 Size: 19 514 577 301 713 270 Fragments arranged by size: 713 577 514 301 270 19 PshAI GACnn'nnGTC Cuts at: 0 896 2394 Size: 896 1498 PstI C_TGCA'G Cuts at: 0 1210 2394 Size: 1210 1184 PvuI CG_AT'CG Cuts at: 0 566 2394 Size: 566 1828 PvuII CAG'CTG Cuts at: 0 2033 2394 Size: 2033 361 SacI G_AGCT'C Cuts at: 0 315 360 2394 Size: 315 45 2034 Fragments arranged by size: 2034 315 45 SanDI GG'GwC_CC Cuts at: 0 533 2394 Size: 533 1861 SapI GCTCTTCn'nnn_ Cuts at: 0 1009 1801 2008 2394 Size: 1009 792 207 386 Fragments arranged by size: 1009 792 386 207 SfcI C'TryA_G Cuts at: 0 1206 2163 2394 Size: 1206 957 231 Fragments arranged by size: 1206 957 231 SmaI CCC'GGG Cuts at: 0 17 531 2394 Size: 17 514 1863 Fragments arranged by size: 1863 514 17 StyI C'CwwG_G Cuts at: 0 263 544 683 956 973 1862 2114 2394 Size: 263 281 139 273 17 889 252 280 Fragments arranged by size: 889 281 280 273 263 252 139 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 653 1120 2394 Size: 653 467 1274 Fragments arranged by size: 1274 653 467 Tth111I GACn'n_nGTC Cuts at: 0 553 2394 Size: 553 1841 XmaI C'CCGG_G Cuts at: 0 15 529 2394 Size: 15 514 1865 Fragments arranged by size: 1865 514 15 XmnI GAAnn'nnTTC Cuts at: 0 1092 1493 2394 Size: 1092 401 901 Fragments arranged by size: 1092 901 401 Enzymes that do cut: AcuI AfeI AgeI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I MfeI MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV PasI PflMI PfoI PpiI PpuMI PshAI PstI PvuI PvuII SacI SanDI SapI SfcI SmaI StyI TaqII Tth111I XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AhdI AleI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI BglI BglII BmgBI BmtI BpuEI BsaI BsaAI BsaXI BsiWI BsmBI BspMI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI CspCI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MluI MscI NarI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PsiI PspOMI PspXI PsrI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XcmI XhoI ZraI