Restriction map for
pFN21AE3306
(Linear) (Six-base) MAPSORT of: KIEE3306.seq Check: 5276 from: 1 to: 2310
KIEE3306.orf
With 182 enzymes: SgfI *
July 3, 2012 17:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1589 2310
Size: 1589 721
AatII G_ACGT'C
Cuts at: 0 1621 2310
Size: 1621 689
AccI GT'mk_AC
Cuts at: 0 1280 2310
Size: 1280 1030
Acc65I G'GTAC_C
Cuts at: 0 1730 2310
Size: 1730 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 1351 1717 1935 2254 2280 2310
Size: 34 1317 366 218 319 26 30
Fragments arranged by size:
1317 366 319 218 34 30 26
AfeI AGC'GCT
Cuts at: 0 1984 2064 2310
Size: 1984 80 246
Fragments arranged by size:
1984 246 80
AflIII A'CryG_T
Cuts at: 0 1784 2310
Size: 1784 526
AhdI GACnn_n'nnGTC
Cuts at: 0 750 1788 2310
Size: 750 1038 522
Fragments arranged by size:
1038 750 522
AleI CACnn'nnGTG
Cuts at: 0 1777 2310
Size: 1777 533
AlwNI CAG_nnn'CTG
Cuts at: 0 653 832 1188 1245 1563 2310
Size: 653 179 356 57 318 747
Fragments arranged by size:
747 653 356 318 179 57
ApaI G_GGCC'C
Cuts at: 0 2290 2310
Size: 2290 20
ApoI r'AATT_y
Cuts at: 0 44 108 2310
Size: 44 64 2202
Fragments arranged by size:
2202 64 44
AvaI C'yCGr_G
Cuts at: 0 1840 2047 2181 2310
Size: 1840 207 134 129
Fragments arranged by size:
1840 207 134 129
BamHI G'GATC_C
Cuts at: 0 1595 1687 2310
Size: 1595 92 623
Fragments arranged by size:
1595 623 92
BanI G'GyrC_C
Cuts at: 0 1264 1730 1756 2173 2310
Size: 1264 466 26 417 137
Fragments arranged by size:
1264 466 417 137 26
BanII G_rGCy'C
Cuts at: 0 1178 1928 1995 2290 2310
Size: 1178 750 67 295 20
Fragments arranged by size:
1178 750 295 67 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 227 793 1549 2310
Size: 215 12 566 756 761
Fragments arranged by size:
761 756 566 215 12
BbvCI CC'TCA_GC
Cuts at: 0 1372 2066 2310
Size: 1372 694 244
Fragments arranged by size:
1372 694 244
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 256 290 343 377 1138 1172 1968 2002
Size: 256 34 53 34 761 34 796 34
Cuts at: 2002 2310
Size: 308
Fragments arranged by size:
796 761 308 256 53 34 34 34
34
BglI GCCn_nnn'nGGC
Cuts at: 0 1643 2310
Size: 1643 667
BglII A'GATC_T
Cuts at: 0 1457 2310
Size: 1457 853
BlpI GC'TnA_GC
Cuts at: 0 1633 2310
Size: 1633 677
Bme1580I G_kGCm'C
Cuts at: 0 1148 1267 2176 2290 2310
Size: 1148 119 909 114 20
Fragments arranged by size:
1148 909 119 114 20
BmgBI CAC'GTC
Cuts at: 0 1367 2310
Size: 1367 943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 2310
Size: 675 1635
Bpu10I CC'TnA_GC
Cuts at: 0 825 1372 2066 2310
Size: 825 547 694 244
Fragments arranged by size:
825 694 547 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 1162 1378 1477 1597 1636 2062 2287
Size: 163 999 216 99 120 39 426 225
Cuts at: 2287 2310
Size: 23
Fragments arranged by size:
999 426 225 216 163 120 99 39
23
BsaI GGTCTCn'nnnn_
Cuts at: 0 925 1501 2092 2310
Size: 925 576 591 218
Fragments arranged by size:
925 591 576 218
BsaAI yAC'GTr
Cuts at: 0 2135 2310
Size: 2135 175
BsaBI GATnn'nnATC
Cuts at: 0 1462 2310
Size: 1462 848
BsaHI Gr'CG_yC
Cuts at: 0 1618 2310
Size: 1618 692
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 867 897 1225 1255 2310
Size: 867 30 328 30 1055
Fragments arranged by size:
1055 867 328 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 160 242 574 859 862 865 874
Size: 94 66 82 332 285 3 3 9
Cuts at: 874 877 880 910 913 916 919 934 937
Size: 3 3 30 3 3 3 15 3
Cuts at: 937 940 943 964 1246 1273 2067 2310
Size: 3 3 21 282 27 794 243
Fragments arranged by size:
794 332 285 282 243 94 82 66
30 27 21 15 9 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 263 1400 1929 2217 2310
Size: 263 1137 529 288 93
Fragments arranged by size:
1137 529 288 263 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 1567 1585 2310
Size: 584 983 18 725
Fragments arranged by size:
983 725 584 18
BsiEI CG_ry'CG
Cuts at: 0 676 708 800 1059 1715 1778 2310
Size: 676 32 92 259 656 63 532
Fragments arranged by size:
676 656 532 259 92 63 32
BsiHKAI G_wGCw'C
Cuts at: 0 356 880 1178 1487 1928 1961 2310
Size: 356 524 298 309 441 33 349
Fragments arranged by size:
524 441 356 349 309 298 33
BsmI GAATG_Cn'
Cuts at: 0 441 2310
Size: 441 1869
BsmBI CGTCTCn'nnnn_
Cuts at: 0 695 2233 2310
Size: 695 1538 77
Fragments arranged by size:
1538 695 77
Bsp1286I G_dGCh'C
Cuts at: 0 356 880 1148 1178 1267 1487 1928 1961
Size: 356 524 268 30 89 220 441 33
Cuts at: 1961 1995 2176 2290 2310
Size: 34 181 114 20
Fragments arranged by size:
524 441 356 268 220 181 114 89
34 33 30 20
BspHI T'CATG_A
Cuts at: 0 2021 2310
Size: 2021 289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 755 1208 1589 1634 2310
Size: 755 453 381 45 676
Fragments arranged by size:
755 676 453 381 45
BsrBI CCG'CTC
Cuts at: 0 492 549 564 576 687 2310
Size: 492 57 15 12 111 1623
Fragments arranged by size:
1623 492 111 57 15 12
BsrFI r'CCGG_y
Cuts at: 0 934 1203 1642 2310
Size: 934 269 439 668
Fragments arranged by size:
934 668 439 269
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1188 2310
Size: 1188 1122
BstEII G'GTnAC_C
Cuts at: 0 1719 2310
Size: 1719 591
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1779 2310
Size: 1779 531
BstYI r'GATC_y
Cuts at: 0 1457 1595 1687 1881 2310
Size: 1457 138 92 194 429
Fragments arranged by size:
1457 429 194 138 92
Bsu36I CC'TnA_GG
Cuts at: 0 2192 2310
Size: 2192 118
BtgI C'CryG_G
Cuts at: 0 679 1772 1778 2282 2310
Size: 679 1093 6 504 28
Fragments arranged by size:
1093 679 504 28 6
ClaI AT'CG_AT
Cuts at: 0 1856 2310
Size: 1856 454
EaeI y'GGCC_r
Cuts at: 0 248 673 705 797 1775 2310
Size: 248 425 32 92 978 535
Fragments arranged by size:
978 535 425 248 92 32
EagI C'GGCC_G
Cuts at: 0 673 705 797 1775 2310
Size: 673 32 92 978 535
Fragments arranged by size:
978 673 535 92 32
EarI CTCTTCn'nnn_
Cuts at: 0 198 412 719 836 851 905 911 2310
Size: 198 214 307 117 15 54 6 1399
Fragments arranged by size:
1399 307 214 198 117 54 15 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 571 2310
Size: 571 1739
EcoICRI GAG'CTC
Cuts at: 0 1176 1926 2310
Size: 1176 750 384
Fragments arranged by size:
1176 750 384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 675 1351 1717 1935 2254 2280 2310
Size: 34 641 676 366 218 319 26 30
Fragments arranged by size:
676 641 366 319 218 34 30 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 145 1129 2195 2310
Size: 145 984 1066 115
Fragments arranged by size:
1066 984 145 115
EcoO109I rG'GnC_Cy
Cuts at: 0 744 1889 2186 2273 2287 2310
Size: 744 1145 297 87 14 23
Fragments arranged by size:
1145 744 297 87 23 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1371 1965 2310
Size: 1371 594 345
Fragments arranged by size:
1371 594 345
HaeII r_GCGC'y
Cuts at: 0 1124 1986 2066 2310
Size: 1124 862 80 244
Fragments arranged by size:
1124 862 244 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 736 768 793 825 940 972 1672 1704
Size: 736 32 25 32 115 32 700 32
Cuts at: 1704 1774 1806 2310
Size: 70 32 504
Fragments arranged by size:
736 700 504 115 70 32 32 32
32 32 25
KpnI G_GTAC'C
Cuts at: 0 1734 2310
Size: 1734 576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 779 1646 2310
Size: 343 436 867 664
Fragments arranged by size:
867 664 436 343
MscI TGG'CCA
Cuts at: 0 250 2310
Size: 250 2060
MslI CAynn'nnrTG
Cuts at: 0 1468 1777 2310
Size: 1468 309 533
Fragments arranged by size:
1468 533 309
MspA1I CmG'CkG
Cuts at: 0 528 681 814 1560 2217 2284 2310
Size: 528 153 133 746 657 67 26
Fragments arranged by size:
746 657 528 153 133 67 26
NaeI GCC'GGC
Cuts at: 0 1205 1644 2310
Size: 1205 439 666
Fragments arranged by size:
1205 666 439
NgoMIV G'CCGG_C
Cuts at: 0 1203 1642 2310
Size: 1203 439 668
Fragments arranged by size:
1203 668 439
NspI r_CATG'y
Cuts at: 0 334 1788 2310
Size: 334 1454 522
Fragments arranged by size:
1454 522 334
PciI A'CATG_T
Cuts at: 0 1784 2310
Size: 1784 526
PflMI CCAn_nnn'nTGG
Cuts at: 0 653 1032 2310
Size: 653 379 1278
Fragments arranged by size:
1278 653 379
PfoI T'CCnGG_A
Cuts at: 0 445 2310
Size: 445 1865
PmlI CAC'GTG
Cuts at: 0 2135 2310
Size: 2135 175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 2310
Size: 1036 32 1242
Fragments arranged by size:
1242 1036 32
PpuMI rG'GwC_Cy
Cuts at: 0 744 1889 2310
Size: 744 1145 421
Fragments arranged by size:
1145 744 421
PspOMI G'GGCC_C
Cuts at: 0 2286 2310
Size: 2286 24
PstI C_TGCA'G
Cuts at: 0 1214 2310
Size: 1214 1096
PvuII CAG'CTG
Cuts at: 0 1560 2217 2310
Size: 1560 657 93
Fragments arranged by size:
1560 657 93
SacI G_AGCT'C
Cuts at: 0 1178 1928 2310
Size: 1178 750 382
Fragments arranged by size:
1178 750 382
SacII CC_GC'GG
Cuts at: 0 682 2285 2310
Size: 682 1603 25
Fragments arranged by size:
1603 682 25
SanDI GG'GwC_CC
Cuts at: 0 1889 2310
Size: 1889 421
SbfI CC_TGCA'GG
Cuts at: 0 1214 2310
Size: 1214 1096
SfcI C'TryA_G
Cuts at: 0 627 1210 1281 1917 2208 2310
Size: 627 583 71 636 291 102
Fragments arranged by size:
636 627 583 291 102 71
SmaI CCC'GGG
Cuts at: 0 1842 2049 2310
Size: 1842 207 261
Fragments arranged by size:
1842 261 207
SmlI C'TyrA_G
Cuts at: 0 142 1177 1393 1492 1612 1651 2077 2302
Size: 142 1035 216 99 120 39 426 225
Cuts at: 2302 2310
Size: 8
Fragments arranged by size:
1035 426 225 216 142 120 99 39
8
SphI G_CATG'C
Cuts at: 0 334 2310
Size: 334 1976
StuI AGG'CCT
Cuts at: 0 1702 2201 2310
Size: 1702 499 109
Fragments arranged by size:
1702 499 109
StyI C'CwwG_G
Cuts at: 0 1760 2310
Size: 1760 550
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1073 1087 1648 2209 2310
Size: 1073 14 561 561 101
Fragments arranged by size:
1073 561 561 101 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 867 899 1225 1257 1881 1913 2310
Size: 867 32 326 32 624 32 397
Fragments arranged by size:
867 624 397 326 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1564 2310
Size: 1564 746
XmaI C'CCGG_G
Cuts at: 0 1840 2047 2310
Size: 1840 207 263
Fragments arranged by size:
1840 263 207
XmnI GAAnn'nnTTC
Cuts at: 0 47 287 2029 2310
Size: 47 240 1742 281
Fragments arranged by size:
1742 281 240 47
ZraI GAC'GTC
Cuts at: 0 1619 2310
Size: 1619 691
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrFI BstAPI BstEII BstXI BstYI
Bsu36I BtgI ClaI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SbfI SfcI SmaI SmlI SphI StuI
StyI TaqII TstI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AgeI AlfI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BmrI
BmtI BplI BsaWI BsiWI BspEI BsrDI BsrGI BssHII
BssSI BstBI BstZ17I BtgZI BtsI CspCI DraI DraIII
DrdI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SapI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TatI Tth111I XbaI XhoI