Restriction map for
pFN21AE3305
(Linear) (Six-base) MAPSORT of: KIEE3305.seq Check: 4352 from: 1 to: 2388
KIEE3305.orf
With 182 enzymes: SgfI *
December 27, 2011 16:35 ..
AccI GT'mk_AC
Cuts at: 0 107 570 1254 2388
Size: 107 463 684 1134
Fragments arranged by size:
1134 684 463 107
Acc65I G'GTAC_C
Cuts at: 0 1980 2388
Size: 1980 408
AclI AA'CG_TT
Cuts at: 0 1088 2388
Size: 1088 1300
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 2272 2388
Size: 184 2088 116
Fragments arranged by size:
2088 184 116
AflIII A'CryG_T
Cuts at: 0 1727 2388
Size: 1727 661
AhdI GACnn_n'nnGTC
Cuts at: 0 566 2388
Size: 566 1822
ApoI r'AATT_y
Cuts at: 0 97 421 444 575 718 756 1236 1299
Size: 97 324 23 131 143 38 480 63
Cuts at: 1299 1615 2388
Size: 316 773
Fragments arranged by size:
773 480 324 316 143 131 97 63
38 23
BanI G'GyrC_C
Cuts at: 0 1774 1980 2132 2388
Size: 1774 206 152 256
Fragments arranged by size:
1774 256 206 152
BanII G_rGCy'C
Cuts at: 0 2315 2388
Size: 2315 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 1298 2008 2388
Size: 1298 710 380
Fragments arranged by size:
1298 710 380
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1185 1219 2388
Size: 1185 34 1169
Fragments arranged by size:
1185 1169 34
BclI T'GATC_A
Cuts at: 0 53 1499 1506 2388
Size: 53 1446 7 882
Fragments arranged by size:
1446 882 53 7
BglII A'GATC_T
Cuts at: 0 378 2388
Size: 378 2010
BlpI GC'TnA_GC
Cuts at: 0 194 2388
Size: 194 2194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1903 2388
Size: 1903 485
BsaI GGTCTCn'nnnn_
Cuts at: 0 210 1192 2388
Size: 210 982 1196
Fragments arranged by size:
1196 982 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1254 2388
Size: 1254 1134
BsmI GAATG_Cn'
Cuts at: 0 588 872 2228 2388
Size: 588 284 1356 160
Fragments arranged by size:
1356 588 284 160
Bsp1286I G_dGCh'C
Cuts at: 0 2315 2388
Size: 2315 73
BspHI T'CATG_A
Cuts at: 0 1190 2019 2172 2261 2388
Size: 1190 829 153 89 127
Fragments arranged by size:
1190 829 153 127 89
BsrGI T'GTAC_A
Cuts at: 0 971 2388
Size: 971 1417
BstXI CCAn_nnnn'nTGG
Cuts at: 0 831 1785 2388
Size: 831 954 603
Fragments arranged by size:
954 831 603
BstYI r'GATC_y
Cuts at: 0 378 1827 2179 2388
Size: 378 1449 352 209
Fragments arranged by size:
1449 378 352 209
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 174 2388
Size: 174 2214
BtsI GCAGTG_nn'
Cuts at: 0 2290 2388
Size: 2290 98
EarI CTCTTCn'nnn_
Cuts at: 0 2074 2388
Size: 2074 314
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 1903 2272 2388
Size: 184 1719 369 116
Fragments arranged by size:
1719 369 184 116
EcoO109I rG'GnC_Cy
Cuts at: 0 402 2388
Size: 402 1986
EcoRI G'AATT_C
Cuts at: 0 1236 2388
Size: 1236 1152
EcoRV GAT'ATC
Cuts at: 0 344 2388
Size: 344 2044
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2112 2388
Size: 2112 276
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 552 584 1491 1523 1604 1624 1636 1656
Size: 552 32 907 32 81 20 12 20
Cuts at: 1656 1854 1886 2368 2388
Size: 198 32 482 20
Fragments arranged by size:
907 552 482 198 81 32 32 32
20 20 20 12
HincII GTy'rAC
Cuts at: 0 233 1007 2388
Size: 233 774 1381
Fragments arranged by size:
1381 774 233
HindIII A'AGCT_T
Cuts at: 0 30 1418 2388
Size: 30 1388 970
Fragments arranged by size:
1388 970 30
HpaI GTT'AAC
Cuts at: 0 1007 2388
Size: 1007 1381
KpnI G_GTAC'C
Cuts at: 0 1984 2388
Size: 1984 404
MfeI C'AATT_G
Cuts at: 0 494 2388
Size: 494 1894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 45 110 769 2388
Size: 45 65 659 1619
Fragments arranged by size:
1619 659 65 45
MslI CAynn'nnrTG
Cuts at: 0 1000 1593 1786 2388
Size: 1000 593 193 602
Fragments arranged by size:
1000 602 593 193
MspA1I CmG'CkG
Cuts at: 0 153 2271 2388
Size: 153 2118 117
Fragments arranged by size:
2118 153 117
NspI r_CATG'y
Cuts at: 0 1038 1731 2388
Size: 1038 693 657
Fragments arranged by size:
1038 693 657
PciI A'CATG_T
Cuts at: 0 1727 2388
Size: 1727 661
PfoI T'CCnGG_A
Cuts at: 0 1464 2388
Size: 1464 924
PshAI GACnn'nnGTC
Cuts at: 0 701 2388
Size: 701 1687
PsiI TTA'TAA
Cuts at: 0 1676 2388
Size: 1676 712
SpeI A'CTAG_T
Cuts at: 0 616 2358 2388
Size: 616 1742 30
Fragments arranged by size:
1742 616 30
SspI AAT'ATT
Cuts at: 0 310 681 846 2388
Size: 310 371 165 1542
Fragments arranged by size:
1542 371 310 165
StyI C'CwwG_G
Cuts at: 0 2291 2388
Size: 2291 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 626 1541 1953 2388
Size: 626 915 412 435
Fragments arranged by size:
915 626 435 412
TatI w'GTAC_w
Cuts at: 0 971 2388
Size: 971 1417
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 726 758 2388
Size: 726 32 1630
Fragments arranged by size:
1630 726 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1788 2388
Size: 1788 600
XmnI GAAnn'nnTTC
Cuts at: 0 872 1090 2213 2388
Size: 872 218 1123 175
Fragments arranged by size:
1123 872 218 175
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI ApoI BanI
BanII BbsI BcgI BclI BglII BlpI BpmI BsaI
BsgI BsmI Bsp1286I BspHI BsrGI BstXI BstYI BtgZI
BtsI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HincII HindIII HpaI KpnI MfeI MmeI MslI MspA1I
NspI PciI PfoI PshAI PsiI SpeI SspI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AleI AlfI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BfrBI BglI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI
FseI FspI FspAI HaeII KasI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI