Restriction map for
pFN21AE3304
(Linear) (Six-base) MAPSORT of: KIEE3304.seq Check: 609 from: 1 to: 2385
KIEE3304.orf
With 182 enzymes: SgfI *
March 22, 2012 16:29 ..
AccI GT'mk_AC
Cuts at: 0 1458 2141 2385
Size: 1458 683 244
Fragments arranged by size:
1458 683 244
Acc65I G'GTAC_C
Cuts at: 0 1048 2385
Size: 1048 1337
AclI AA'CG_TT
Cuts at: 0 1272 2039 2385
Size: 1272 767 346
Fragments arranged by size:
1272 767 346
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 86 220 1950 2231 2385
Size: 63 23 134 1730 281 154
Fragments arranged by size:
1730 281 154 134 63 23
AflIII A'CryG_T
Cuts at: 0 114 1356 1754 2385
Size: 114 1242 398 631
Fragments arranged by size:
1242 631 398 114
AleI CACnn'nnGTG
Cuts at: 0 191 2385
Size: 191 2194
AlwNI CAG_nnn'CTG
Cuts at: 0 545 2150 2385
Size: 545 1605 235
Fragments arranged by size:
1605 545 235
ApoI r'AATT_y
Cuts at: 0 1928 1945 2385
Size: 1928 17 440
Fragments arranged by size:
1928 440 17
AvaI C'yCGr_G
Cuts at: 0 248 426 2243 2385
Size: 248 178 1817 142
Fragments arranged by size:
1817 248 178 142
BanI G'GyrC_C
Cuts at: 0 1048 2385
Size: 1048 1337
BanII G_rGCy'C
Cuts at: 0 692 2385
Size: 692 1693
BbsI GAAGACnn'nnnn_
Cuts at: 0 69 2385
Size: 69 2316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 196 1359 1393 2385
Size: 162 34 1163 34 992
Fragments arranged by size:
1163 992 162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 103 2385
Size: 103 2282
BclI T'GATC_A
Cuts at: 0 1661 2385
Size: 1661 724
BfrBI ATG'CAT
Cuts at: 0 2021 2385
Size: 2021 364
BglI GCCn_nnn'nGGC
Cuts at: 0 757 949 2385
Size: 757 192 1436
Fragments arranged by size:
1436 757 192
BglII A'GATC_T
Cuts at: 0 82 1629 1656 2385
Size: 82 1547 27 729
Fragments arranged by size:
1547 729 82 27
BlpI GC'TnA_GC
Cuts at: 0 686 2385
Size: 686 1699
Bme1580I G_kGCm'C
Cuts at: 0 1013 1736 2385
Size: 1013 723 649
Fragments arranged by size:
1013 723 649
BmgBI CAC'GTC
Cuts at: 0 1014 1357 2385
Size: 1014 343 1028
Fragments arranged by size:
1028 1014 343
BmrI ACTGGGnnnn_n'
Cuts at: 0 528 2212 2385
Size: 528 1684 173
Fragments arranged by size:
1684 528 173
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1587 2385
Size: 1587 798
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 2385
Size: 1136 1249
Bpu10I CC'TnA_GC
Cuts at: 0 1145 1712 1873 2385
Size: 1145 567 161 512
Fragments arranged by size:
1145 567 512 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2281 2385
Size: 2281 104
BsaI GGTCTCn'nnnn_
Cuts at: 0 2316 2385
Size: 2316 69
BsaAI yAC'GTr
Cuts at: 0 1672 2139 2307 2385
Size: 1672 467 168 78
Fragments arranged by size:
1672 467 168 78
BsaWI w'CCGG_w
Cuts at: 0 1538 2385
Size: 1538 847
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1837 1867 2198 2228 2385
Size: 1837 30 331 30 157
Fragments arranged by size:
1837 331 157 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1249 1250 1326 2385
Size: 1249 1 76 1059
Fragments arranged by size:
1249 1059 76 1
BseYI C'CCAG_C
Cuts at: 0 681 1682 2385
Size: 681 1001 703
Fragments arranged by size:
1001 703 681
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1511 2385
Size: 503 1008 874
Fragments arranged by size:
1008 874 503
BsmI GAATG_Cn'
Cuts at: 0 2169 2385
Size: 2169 216
Bsp1286I G_dGCh'C
Cuts at: 0 692 1013 1736 2385
Size: 692 321 723 649
Fragments arranged by size:
723 692 649 321
BspHI T'CATG_A
Cuts at: 0 621 2349 2385
Size: 621 1728 36
Fragments arranged by size:
1728 621 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 291 710 2385
Size: 291 419 1675
Fragments arranged by size:
1675 419 291
BsrDI GCAATG_nn'
Cuts at: 0 1500 2385
Size: 1500 885
BsrGI T'GTAC_A
Cuts at: 0 67 2385
Size: 67 2318
BssSI C'ACGA_G
Cuts at: 0 473 2385
Size: 473 1912
BstEII G'GTnAC_C
Cuts at: 0 243 342 564 1788 2188 2385
Size: 243 99 222 1224 400 197
Fragments arranged by size:
1224 400 243 222 197 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1389 2071 2385
Size: 1389 682 314
Fragments arranged by size:
1389 682 314
BstYI r'GATC_y
Cuts at: 0 82 389 1205 1629 1656 1745 1866 2385
Size: 82 307 816 424 27 89 121 519
Fragments arranged by size:
816 519 424 307 121 89 82 27
BtgI C'CryG_G
Cuts at: 0 239 1578 2385
Size: 239 1339 807
Fragments arranged by size:
1339 807 239
BtsI GCAGTG_nn'
Cuts at: 0 198 240 2385
Size: 198 42 2145
Fragments arranged by size:
2145 198 42
DraI TTT'AAA
Cuts at: 0 2339 2385
Size: 2339 46
DrdI GACnn_nn'nnGTC
Cuts at: 0 2308 2385
Size: 2308 77
EaeI y'GGCC_r
Cuts at: 0 1130 1307 2385
Size: 1130 177 1078
Fragments arranged by size:
1130 1078 177
EarI CTCTTCn'nnn_
Cuts at: 0 1683 2385
Size: 1683 702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 63 86 220 1136 1950 2231 2385
Size: 63 23 134 916 814 281 154
Fragments arranged by size:
916 814 281 154 134 63 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1558 2385
Size: 1558 827
EcoO109I rG'GnC_Cy
Cuts at: 0 417 1078 1939 2007 2385
Size: 417 661 861 68 378
Fragments arranged by size:
861 661 417 378 68
HaeII r_GCGC'y
Cuts at: 0 282 600 2385
Size: 282 318 1785
Fragments arranged by size:
1785 318 282
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 289 321 862 894 2385
Size: 289 32 541 32 1491
Fragments arranged by size:
1491 541 289 32 32
HincII GTy'rAC
Cuts at: 0 657 1187 2385
Size: 657 530 1198
Fragments arranged by size:
1198 657 530
HindIII A'AGCT_T
Cuts at: 0 844 2385
Size: 844 1541
KpnI G_GTAC'C
Cuts at: 0 1052 2385
Size: 1052 1333
MfeI C'AATT_G
Cuts at: 0 1415 2385
Size: 1415 970
MscI TGG'CCA
Cuts at: 0 1132 1309 2385
Size: 1132 177 1076
Fragments arranged by size:
1132 1076 177
MslI CAynn'nnrTG
Cuts at: 0 191 1757 1759 1903 2069 2087 2385
Size: 191 1566 2 144 166 18 298
Fragments arranged by size:
1566 298 191 166 144 18 2
MspA1I CmG'CkG
Cuts at: 0 20 441 487 685 1686 1828 2119 2385
Size: 20 421 46 198 1001 142 291 266
Fragments arranged by size:
1001 421 291 266 198 142 46 20
NcoI C'CATG_G
Cuts at: 0 1578 2385
Size: 1578 807
NsiI A_TGCA'T
Cuts at: 0 2023 2385
Size: 2023 362
NspI r_CATG'y
Cuts at: 0 118 208 1758 2385
Size: 118 90 1550 627
Fragments arranged by size:
1550 627 118 90
PasI CC'CwG_GG
Cuts at: 0 56 273 2385
Size: 56 217 2112
Fragments arranged by size:
2112 217 56
PciI A'CATG_T
Cuts at: 0 114 1754 2385
Size: 114 1640 631
Fragments arranged by size:
1640 631 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 561 1173 2385
Size: 561 612 1212
Fragments arranged by size:
1212 612 561
PfoI T'CCnGG_A
Cuts at: 0 864 2385
Size: 864 1521
PmlI CAC'GTG
Cuts at: 0 1672 2307 2385
Size: 1672 635 78
Fragments arranged by size:
1672 635 78
PpuMI rG'GwC_Cy
Cuts at: 0 2007 2385
Size: 2007 378
PstI C_TGCA'G
Cuts at: 0 317 707 1077 2135 2385
Size: 317 390 370 1058 250
Fragments arranged by size:
1058 390 370 317 250
PvuII CAG'CTG
Cuts at: 0 441 487 685 1686 1828 2385
Size: 441 46 198 1001 142 557
Fragments arranged by size:
1001 557 441 198 142 46
SapI GCTCTTCn'nnn_
Cuts at: 0 1683 2385
Size: 1683 702
SbfI CC_TGCA'GG
Cuts at: 0 707 1077 2385
Size: 707 370 1308
Fragments arranged by size:
1308 707 370
SfcI C'TryA_G
Cuts at: 0 313 703 1073 2131 2385
Size: 313 390 370 1058 254
Fragments arranged by size:
1058 390 370 313 254
SmaI CCC'GGG
Cuts at: 0 2245 2385
Size: 2245 140
SmlI C'TyrA_G
Cuts at: 0 2260 2385
Size: 2260 125
SphI G_CATG'C
Cuts at: 0 208 2385
Size: 208 2177
StuI AGG'CCT
Cuts at: 0 809 2385
Size: 809 1576
StyI C'CwwG_G
Cuts at: 0 929 1136 1578 2385
Size: 929 207 442 807
Fragments arranged by size:
929 807 442 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 904 1010 1303 2385
Size: 904 106 293 1082
Fragments arranged by size:
1082 904 293 106
TatI w'GTAC_w
Cuts at: 0 67 536 2224 2385
Size: 67 469 1688 161
Fragments arranged by size:
1688 469 161 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2198 2230 2385
Size: 2198 32 155
Fragments arranged by size:
2198 155 32
XbaI T'CTAG_A
Cuts at: 0 901 2385
Size: 901 1484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 903 2385
Size: 216 687 1482
Fragments arranged by size:
1482 687 216
XmaI C'CCGG_G
Cuts at: 0 2243 2385
Size: 2243 142
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlwNI ApoI
AvaI BanI BanII BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI
BstEII BstXI BstYI BtgI BtsI DraI DrdI EaeI
EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
KpnI MfeI MscI MslI MspA1I NcoI NsiI NspI
PasI PciI PflMI PfoI PmlI PpuMI PstI PvuII
SapI SbfI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BmtI BsaBI BsaHI BsiEI BsiHKAI BsiWI
BsmBI BspEI BsrBI BsrFI BssHII BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraIII EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HpaI KasI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PmeI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XhoI XmnI ZraI