Restriction map for
pFN21AE3290
(Linear) (Six-base) MAPSORT of: KIEE3290.seq Check: 6093 from: 1 to: 2241
KIEE3290.orf
With 182 enzymes: SgfI *
July 3, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 609 2241
Size: 609 1632
AccI GT'mk_AC
Cuts at: 0 576 2241
Size: 576 1665
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 1828 2241
Size: 181 1647 413
Fragments arranged by size:
1647 413 181
AgeI A'CCGG_T
Cuts at: 0 1521 2241
Size: 1521 720
AlwNI CAG_nnn'CTG
Cuts at: 0 1942 2019 2169 2196 2241
Size: 1942 77 150 27 45
Fragments arranged by size:
1942 150 77 45 27
ApaI G_GGCC'C
Cuts at: 0 1247 2241
Size: 1247 994
ApaLI G'TGCA_C
Cuts at: 0 1130 2241
Size: 1130 1111
ApoI r'AATT_y
Cuts at: 0 458 700 1201 1366 1481 2218 2241
Size: 458 242 501 165 115 737 23
Fragments arranged by size:
737 501 458 242 165 115 23
AvaI C'yCGr_G
Cuts at: 0 884 1180 1625 2241
Size: 884 296 445 616
Fragments arranged by size:
884 616 445 296
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2143 2176 2241
Size: 2143 33 65
Fragments arranged by size:
2143 65 33
BanI G'GyrC_C
Cuts at: 0 1560 1686 2241
Size: 1560 126 555
Fragments arranged by size:
1560 555 126
BanII G_rGCy'C
Cuts at: 0 1181 1247 1910 2241
Size: 1181 66 663 331
Fragments arranged by size:
1181 663 331 66
BbsI GAAGACnn'nnnn_
Cuts at: 0 1001 2241
Size: 1001 1240
BbvCI CC'TCA_GC
Cuts at: 0 1172 1943 2036 2241
Size: 1172 771 93 205
Fragments arranged by size:
1172 771 205 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2219 2241
Size: 2219 22
BfrBI ATG'CAT
Cuts at: 0 550 2241
Size: 550 1691
BglII A'GATC_T
Cuts at: 0 358 2241
Size: 358 1883
Bme1580I G_kGCm'C
Cuts at: 0 977 1134 1247 1691 2241
Size: 977 157 113 444 550
Fragments arranged by size:
977 550 444 157 113
BmrI ACTGGGnnnn_n'
Cuts at: 0 145 2241
Size: 145 2096
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 377 2241
Size: 377 1864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 323 664 1186 2041 2191 2241
Size: 173 150 341 522 855 150 50
Fragments arranged by size:
855 522 341 173 150 150 50
Bpu10I CC'TnA_GC
Cuts at: 0 1172 1943 2036 2241
Size: 1172 771 93 205
Fragments arranged by size:
1172 771 205 93
BsaI GGTCTCn'nnnn_
Cuts at: 0 1088 2241
Size: 1088 1153
BsaAI yAC'GTr
Cuts at: 0 1213 2241
Size: 1213 1028
BsaWI w'CCGG_w
Cuts at: 0 1521 2241
Size: 1521 720
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 115 145 2164 2194 2241
Size: 115 30 2019 30 47
Fragments arranged by size:
2019 115 47 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 646 1047 1189 2083 2119 2241
Size: 121 525 401 142 894 36 122
Fragments arranged by size:
894 525 401 142 122 121 36
BseYI C'CCAG_C
Cuts at: 0 344 2057 2241
Size: 344 1713 184
Fragments arranged by size:
1713 344 184
BsiHKAI G_wGCw'C
Cuts at: 0 525 1134 1181 1806 2241
Size: 525 609 47 625 435
Fragments arranged by size:
625 609 525 435 47
BsmI GAATG_Cn'
Cuts at: 0 552 1493 2241
Size: 552 941 748
Fragments arranged by size:
941 748 552
Bsp1286I G_dGCh'C
Cuts at: 0 525 977 1134 1181 1247 1691 1806 1910
Size: 525 452 157 47 66 444 115 104
Cuts at: 1910 2241
Size: 331
Fragments arranged by size:
525 452 444 331 157 115 104 66
47
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 609 1153 1742 2241
Size: 609 544 589 499
Fragments arranged by size:
609 589 544 499
BsrBI CCG'CTC
Cuts at: 0 2010 2241
Size: 2010 231
BsrFI r'CCGG_y
Cuts at: 0 1521 2241
Size: 1521 720
BstBI TT'CG_AA
Cuts at: 0 1485 2241
Size: 1485 756
BstEII G'GTnAC_C
Cuts at: 0 759 2241
Size: 759 1482
BstYI r'GATC_y
Cuts at: 0 358 2241
Size: 358 1883
BtsI GCAGTG_nn'
Cuts at: 0 525 2235 2241
Size: 525 1710 6
Fragments arranged by size:
1710 525 6
ClaI AT'CG_AT
Cuts at: 0 452 2241
Size: 452 1789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1577 1612 2241
Size: 1577 35 629
Fragments arranged by size:
1577 629 35
EaeI y'GGCC_r
Cuts at: 0 2111 2241
Size: 2111 130
EarI CTCTTCn'nnn_
Cuts at: 0 643 1505 1791 1990 2020 2084 2241
Size: 643 862 286 199 30 64 157
Fragments arranged by size:
862 643 286 199 157 64 30
EcoICRI GAG'CTC
Cuts at: 0 1179 2241
Size: 1179 1062
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 181 323 664 1186 1828 2041 2191
Size: 173 8 142 341 522 642 213 150
Cuts at: 2191 2241
Size: 50
Fragments arranged by size:
642 522 341 213 173 150 142 50
8
EcoO109I rG'GnC_Cy
Cuts at: 0 1947 2241
Size: 1947 294
EcoRI G'AATT_C
Cuts at: 0 700 1481 2241
Size: 700 781 760
Fragments arranged by size:
781 760 700
EcoRV GAT'ATC
Cuts at: 0 1024 2241
Size: 1024 1217
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 283 2241
Size: 283 1958
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 22 54 1318 1350 1521 1553 1918 1950
Size: 22 32 1264 32 171 32 365 32
Cuts at: 1950 2164 2196 2241
Size: 214 32 45
Fragments arranged by size:
1264 365 214 171 45 32 32 32
32 32 22
HincII GTy'rAC
Cuts at: 0 231 774 2241
Size: 231 543 1467
Fragments arranged by size:
1467 543 231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2045 2241
Size: 2045 196
MscI TGG'CCA
Cuts at: 0 2113 2241
Size: 2113 128
MspA1I CmG'CkG
Cuts at: 0 1833 1983 2241
Size: 1833 150 258
Fragments arranged by size:
1833 258 150
NdeI CA'TA_TG
Cuts at: 0 969 2241
Size: 969 1272
NsiI A_TGCA'T
Cuts at: 0 552 2241
Size: 552 1689
PflMI CCAn_nnn'nTGG
Cuts at: 0 1555 2064 2169 2241
Size: 1555 509 105 72
Fragments arranged by size:
1555 509 105 72
PfoI T'CCnGG_A
Cuts at: 0 1322 2241
Size: 1322 919
PmlI CAC'GTG
Cuts at: 0 1213 2241
Size: 1213 1028
PspOMI G'GGCC_C
Cuts at: 0 1243 2241
Size: 1243 998
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1628 1660 2241
Size: 1628 32 581
Fragments arranged by size:
1628 581 32
PstI C_TGCA'G
Cuts at: 0 1676 2241
Size: 1676 565
PvuII CAG'CTG
Cuts at: 0 1833 1983 2241
Size: 1833 150 258
Fragments arranged by size:
1833 258 150
SacI G_AGCT'C
Cuts at: 0 1181 2241
Size: 1181 1060
SapI GCTCTTCn'nnn_
Cuts at: 0 2084 2241
Size: 2084 157
ScaI AGT'ACT
Cuts at: 0 627 2241
Size: 627 1614
SfcI C'TryA_G
Cuts at: 0 1672 2080 2241
Size: 1672 408 161
Fragments arranged by size:
1672 408 161
SmlI C'TyrA_G
Cuts at: 0 1180 1625 2241
Size: 1180 445 616
Fragments arranged by size:
1180 616 445
StyI C'CwwG_G
Cuts at: 0 62 569 930 2241
Size: 62 507 361 1311
Fragments arranged by size:
1311 507 361 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1293 1402 2241
Size: 1293 109 839
Fragments arranged by size:
1293 839 109
TatI w'GTAC_w
Cuts at: 0 625 1110 1153 2241
Size: 625 485 43 1088
Fragments arranged by size:
1088 625 485 43
XhoI C'TCGA_G
Cuts at: 0 1180 1625 2241
Size: 1180 445 616
Fragments arranged by size:
1180 616 445
XmnI GAAnn'nnTTC
Cuts at: 0 1976 2241
Size: 1976 265
Enzymes that do cut:
AarI AccI AcuI AgeI AlwNI ApaI ApaLI ApoI
AvaI BaeI BanI BanII BbsI BbvCI BcgI BfrBI
BglII Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BstBI BstEII BstYI BtsI ClaI CspCI
EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
Hin4I HincII MmeI MscI MspA1I NdeI NsiI PflMI
PfoI PmlI PspOMI PsrI PstI PvuII SacI SapI
ScaI SfcI SmlI StyI TaqII TatI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AhdI AleI
AlfI AloI AscI AseI AsiSI AvrII BamHI BbeI
BciVI BclI BglI BlpI BmgBI BmtI BpuEI BsaBI
BsaHI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BsrGI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgI
BtgZI DraI DraIII DrdI EagI EciI EcoNI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MslI NaeI NarI NcoI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PmeI PpiI PpuMI
PshAI PsiI PspXI PvuI RsrII SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XmaI ZraI