(Linear) (Six-base) MAPSORT of: KIEE3289.seq Check: 8610 from: 1 to: 2235 KIEE3289.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AccI GT'mk_AC Cuts at: 0 45 1109 2235 Size: 45 1064 1126 Fragments arranged by size: 1126 1064 45 Acc65I G'GTAC_C Cuts at: 0 1529 2235 Size: 1529 706 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1418 1424 1875 1936 2235 Size: 1418 6 451 61 299 Fragments arranged by size: 1418 451 299 61 6 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1886 2235 Size: 1886 349 ApaLI G'TGCA_C Cuts at: 0 539 2235 Size: 539 1696 ApoI r'AATT_y Cuts at: 0 903 1591 2007 2077 2235 Size: 903 688 416 70 158 Fragments arranged by size: 903 688 416 158 70 BanI G'GyrC_C Cuts at: 0 1529 1661 2235 Size: 1529 132 574 Fragments arranged by size: 1529 574 132 BbsI GAAGACnn'nnnn_ Cuts at: 0 1837 2235 Size: 1837 398 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 158 192 321 355 2235 Size: 158 34 129 34 1880 Fragments arranged by size: 1880 158 129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 456 1926 2006 2235 Size: 456 1470 80 229 Fragments arranged by size: 1470 456 229 80 BclI T'GATC_A Cuts at: 0 1274 1449 2235 Size: 1274 175 786 Fragments arranged by size: 1274 786 175 BglII A'GATC_T Cuts at: 0 1269 2235 Size: 1269 966 Bme1580I G_kGCm'C Cuts at: 0 543 1666 2235 Size: 543 1123 569 Fragments arranged by size: 1123 569 543 BmgBI CAC'GTC Cuts at: 0 1122 2235 Size: 1122 1113 BmrI ACTGGGnnnn_n' Cuts at: 0 771 2235 Size: 771 1464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1295 2235 Size: 1295 940 Bpu10I CC'TnA_GC Cuts at: 0 2092 2235 Size: 2092 143 BsaBI GATnn'nnATC Cuts at: 0 143 1352 2235 Size: 143 1209 883 Fragments arranged by size: 1209 883 143 BsaWI w'CCGG_w Cuts at: 0 308 2235 Size: 308 1927 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 935 965 2235 Size: 935 30 1270 Fragments arranged by size: 1270 935 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 19 22 736 2235 Size: 19 3 714 1499 Fragments arranged by size: 1499 714 19 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 134 2235 Size: 134 2101 BsiHKAI G_wGCw'C Cuts at: 0 543 2235 Size: 543 1692 BsmI GAATG_Cn' Cuts at: 0 18 777 2235 Size: 18 759 1458 Fragments arranged by size: 1458 759 18 Bsp1286I G_dGCh'C Cuts at: 0 543 1666 2235 Size: 543 1123 569 Fragments arranged by size: 1123 569 543 BsrDI GCAATG_nn' Cuts at: 0 1285 2235 Size: 1285 950 BssSI C'ACGA_G Cuts at: 0 1958 2180 2235 Size: 1958 222 55 Fragments arranged by size: 1958 222 55 BstXI CCAn_nnnn'nTGG Cuts at: 0 576 2235 Size: 576 1659 BstYI r'GATC_y Cuts at: 0 1245 1269 2235 Size: 1245 24 966 Fragments arranged by size: 1245 966 24 BtgI C'CryG_G Cuts at: 0 209 2235 Size: 209 2026 BtsI GCAGTG_nn' Cuts at: 0 1984 2235 Size: 1984 251 ClaI AT'CG_AT Cuts at: 0 285 2235 Size: 285 1950 DraI TTT'AAA Cuts at: 0 2222 2235 Size: 2222 13 DrdI GACnn_nn'nnGTC Cuts at: 0 155 2235 Size: 155 2080 EaeI y'GGCC_r Cuts at: 0 289 2235 Size: 289 1946 EarI CTCTTCn'nnn_ Cuts at: 0 380 2235 Size: 380 1855 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1295 1418 1424 1875 1936 2235 Size: 1295 123 6 451 61 299 Fragments arranged by size: 1295 451 299 123 61 6 EcoO109I rG'GnC_Cy Cuts at: 0 1857 2235 Size: 1857 378 EcoRV GAT'ATC Cuts at: 0 594 2235 Size: 594 1641 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 730 2235 Size: 730 1505 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 81 113 298 330 430 449 462 481 Size: 81 32 185 32 100 19 13 19 Cuts at: 481 514 546 1126 1158 2235 Size: 33 32 580 32 1077 Fragments arranged by size: 1077 580 185 100 81 33 32 32 32 32 19 19 13 HindIII A'AGCT_T Cuts at: 0 678 2235 Size: 678 1557 KpnI G_GTAC'C Cuts at: 0 1533 2235 Size: 1533 702 MfeI C'AATT_G Cuts at: 0 355 2235 Size: 355 1880 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1078 2235 Size: 1078 1157 MscI TGG'CCA Cuts at: 0 291 2235 Size: 291 1944 MslI CAynn'nnrTG Cuts at: 0 421 503 622 716 2169 2235 Size: 421 82 119 94 1453 66 Fragments arranged by size: 1453 421 119 94 82 66 NcoI C'CATG_G Cuts at: 0 209 2235 Size: 209 2026 NdeI CA'TA_TG Cuts at: 0 318 2235 Size: 318 1917 NspI r_CATG'y Cuts at: 0 502 627 2235 Size: 502 125 1608 Fragments arranged by size: 1608 502 125 PfoI T'CCnGG_A Cuts at: 0 1311 2235 Size: 1311 924 PpuMI rG'GwC_Cy Cuts at: 0 1857 2235 Size: 1857 378 PstI C_TGCA'G Cuts at: 0 17 1865 1979 2235 Size: 17 1848 114 256 Fragments arranged by size: 1848 256 114 17 SapI GCTCTTCn'nnn_ Cuts at: 0 380 2235 Size: 380 1855 ScaI AGT'ACT Cuts at: 0 2049 2235 Size: 2049 186 SfcI C'TryA_G Cuts at: 0 13 557 1781 1861 1975 2235 Size: 13 544 1224 80 114 260 Fragments arranged by size: 1224 544 260 114 80 13 SspI AAT'ATT Cuts at: 0 1400 2235 Size: 1400 835 StyI C'CwwG_G Cuts at: 0 209 368 1260 2235 Size: 209 159 892 975 Fragments arranged by size: 975 892 209 159 TatI w'GTAC_w Cuts at: 0 268 1411 2047 2229 2235 Size: 268 1143 636 182 6 Fragments arranged by size: 1143 636 268 182 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 66 98 935 967 1645 1677 2235 Size: 66 32 837 32 678 32 558 Fragments arranged by size: 837 678 558 66 32 32 32 XbaI T'CTAG_A Cuts at: 0 237 2235 Size: 237 1998 Enzymes that do cut: AccI Acc65I AcuI AlfI ApaLI ApoI BanI BbsI BcgI BciVI BclI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrDI BssSI BstXI BstYI BtgI BtsI ClaI DraI DrdI EaeI EarI Eco57MI EcoO109I EcoRV FalI Hin4I HindIII KpnI MfeI MmeI MscI MslI NcoI NdeI NspI PfoI PpuMI PstI SapI ScaI SfcI SspI StyI TatI TstI XbaI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BfrBI BglI BlpI BmtI BplI BpuEI BsaI BsaAI BsaHI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraIII EagI EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HaeII HincII HpaI KasI MluI MspA1I NaeI NarI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I XcmI XhoI XmaI XmnI ZraI