Restriction map for
pFN21AE3289
(Linear) (Six-base) MAPSORT of: KIEE3289.seq Check: 8610 from: 1 to: 2235
KIEE3289.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AccI GT'mk_AC
Cuts at: 0 45 1109 2235
Size: 45 1064 1126
Fragments arranged by size:
1126 1064 45
Acc65I G'GTAC_C
Cuts at: 0 1529 2235
Size: 1529 706
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1418 1424 1875 1936 2235
Size: 1418 6 451 61 299
Fragments arranged by size:
1418 451 299 61 6
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1886 2235
Size: 1886 349
ApaLI G'TGCA_C
Cuts at: 0 539 2235
Size: 539 1696
ApoI r'AATT_y
Cuts at: 0 903 1591 2007 2077 2235
Size: 903 688 416 70 158
Fragments arranged by size:
903 688 416 158 70
BanI G'GyrC_C
Cuts at: 0 1529 1661 2235
Size: 1529 132 574
Fragments arranged by size:
1529 574 132
BbsI GAAGACnn'nnnn_
Cuts at: 0 1837 2235
Size: 1837 398
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 158 192 321 355 2235
Size: 158 34 129 34 1880
Fragments arranged by size:
1880 158 129 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 456 1926 2006 2235
Size: 456 1470 80 229
Fragments arranged by size:
1470 456 229 80
BclI T'GATC_A
Cuts at: 0 1274 1449 2235
Size: 1274 175 786
Fragments arranged by size:
1274 786 175
BglII A'GATC_T
Cuts at: 0 1269 2235
Size: 1269 966
Bme1580I G_kGCm'C
Cuts at: 0 543 1666 2235
Size: 543 1123 569
Fragments arranged by size:
1123 569 543
BmgBI CAC'GTC
Cuts at: 0 1122 2235
Size: 1122 1113
BmrI ACTGGGnnnn_n'
Cuts at: 0 771 2235
Size: 771 1464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 2235
Size: 1295 940
Bpu10I CC'TnA_GC
Cuts at: 0 2092 2235
Size: 2092 143
BsaBI GATnn'nnATC
Cuts at: 0 143 1352 2235
Size: 143 1209 883
Fragments arranged by size:
1209 883 143
BsaWI w'CCGG_w
Cuts at: 0 308 2235
Size: 308 1927
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 935 965 2235
Size: 935 30 1270
Fragments arranged by size:
1270 935 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 22 736 2235
Size: 19 3 714 1499
Fragments arranged by size:
1499 714 19 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 2235
Size: 134 2101
BsiHKAI G_wGCw'C
Cuts at: 0 543 2235
Size: 543 1692
BsmI GAATG_Cn'
Cuts at: 0 18 777 2235
Size: 18 759 1458
Fragments arranged by size:
1458 759 18
Bsp1286I G_dGCh'C
Cuts at: 0 543 1666 2235
Size: 543 1123 569
Fragments arranged by size:
1123 569 543
BsrDI GCAATG_nn'
Cuts at: 0 1285 2235
Size: 1285 950
BssSI C'ACGA_G
Cuts at: 0 1958 2180 2235
Size: 1958 222 55
Fragments arranged by size:
1958 222 55
BstXI CCAn_nnnn'nTGG
Cuts at: 0 576 2235
Size: 576 1659
BstYI r'GATC_y
Cuts at: 0 1245 1269 2235
Size: 1245 24 966
Fragments arranged by size:
1245 966 24
BtgI C'CryG_G
Cuts at: 0 209 2235
Size: 209 2026
BtsI GCAGTG_nn'
Cuts at: 0 1984 2235
Size: 1984 251
ClaI AT'CG_AT
Cuts at: 0 285 2235
Size: 285 1950
DraI TTT'AAA
Cuts at: 0 2222 2235
Size: 2222 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 155 2235
Size: 155 2080
EaeI y'GGCC_r
Cuts at: 0 289 2235
Size: 289 1946
EarI CTCTTCn'nnn_
Cuts at: 0 380 2235
Size: 380 1855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 1418 1424 1875 1936 2235
Size: 1295 123 6 451 61 299
Fragments arranged by size:
1295 451 299 123 61 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1857 2235
Size: 1857 378
EcoRV GAT'ATC
Cuts at: 0 594 2235
Size: 594 1641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 730 2235
Size: 730 1505
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 81 113 298 330 430 449 462 481
Size: 81 32 185 32 100 19 13 19
Cuts at: 481 514 546 1126 1158 2235
Size: 33 32 580 32 1077
Fragments arranged by size:
1077 580 185 100 81 33 32 32
32 32 19 19 13
HindIII A'AGCT_T
Cuts at: 0 678 2235
Size: 678 1557
KpnI G_GTAC'C
Cuts at: 0 1533 2235
Size: 1533 702
MfeI C'AATT_G
Cuts at: 0 355 2235
Size: 355 1880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1078 2235
Size: 1078 1157
MscI TGG'CCA
Cuts at: 0 291 2235
Size: 291 1944
MslI CAynn'nnrTG
Cuts at: 0 421 503 622 716 2169 2235
Size: 421 82 119 94 1453 66
Fragments arranged by size:
1453 421 119 94 82 66
NcoI C'CATG_G
Cuts at: 0 209 2235
Size: 209 2026
NdeI CA'TA_TG
Cuts at: 0 318 2235
Size: 318 1917
NspI r_CATG'y
Cuts at: 0 502 627 2235
Size: 502 125 1608
Fragments arranged by size:
1608 502 125
PfoI T'CCnGG_A
Cuts at: 0 1311 2235
Size: 1311 924
PpuMI rG'GwC_Cy
Cuts at: 0 1857 2235
Size: 1857 378
PstI C_TGCA'G
Cuts at: 0 17 1865 1979 2235
Size: 17 1848 114 256
Fragments arranged by size:
1848 256 114 17
SapI GCTCTTCn'nnn_
Cuts at: 0 380 2235
Size: 380 1855
ScaI AGT'ACT
Cuts at: 0 2049 2235
Size: 2049 186
SfcI C'TryA_G
Cuts at: 0 13 557 1781 1861 1975 2235
Size: 13 544 1224 80 114 260
Fragments arranged by size:
1224 544 260 114 80 13
SspI AAT'ATT
Cuts at: 0 1400 2235
Size: 1400 835
StyI C'CwwG_G
Cuts at: 0 209 368 1260 2235
Size: 209 159 892 975
Fragments arranged by size:
975 892 209 159
TatI w'GTAC_w
Cuts at: 0 268 1411 2047 2229 2235
Size: 268 1143 636 182 6
Fragments arranged by size:
1143 636 268 182 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 66 98 935 967 1645 1677 2235
Size: 66 32 837 32 678 32 558
Fragments arranged by size:
837 678 558 66 32 32 32
XbaI T'CTAG_A
Cuts at: 0 237 2235
Size: 237 1998
Enzymes that do cut:
AccI Acc65I AcuI AlfI ApaLI ApoI BanI BbsI
BcgI BciVI BclI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI
Bsp1286I BsrDI BssSI BstXI BstYI BtgI BtsI ClaI
DraI DrdI EaeI EarI Eco57MI EcoO109I EcoRV FalI
Hin4I HindIII KpnI MfeI MmeI MscI MslI NcoI
NdeI NspI PfoI PpuMI PstI SapI ScaI SfcI
SspI StyI TatI TstI XbaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanII BbeI BbvCI BfrBI BglI
BlpI BmtI BplI BpuEI BsaI BsaAI BsaHI BseYI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
CspCI DraIII EagI EciI EcoICRI EcoNI EcoRI FseI
FspI FspAI HaeII HincII HpaI KasI MluI MspA1I
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI StuI SwaI
TaqII Tth111I XcmI XhoI XmaI XmnI ZraI